Improved measurement method of antagonistic bacteria

Abstract

The invention provides an improved bioassay method for an antagonistic bacterium, namely a double-layer culture medium filter paper sandwich method, which belongs to the technical field of a bioassay method for a strain to be tested which is required to be adopted for biological pesticide development. The antibacterial activity of the antagonistic bacterium on plant pathogenic fungi is determined through the simple and easy procedures of manufacture of a sterile filter paper sheet die, manufacture of an upper culture medium and a lower culture medium, shift-in and shift-out of the sterile filter paper die, culture of the antagonistic bacterium to be tested and the target pathogenic fungi, measurement of experimental results and so on, and the antagonistic bacterium with application value can be reasonable screened out. The simple, convenient and quick novel method for implementing bioassay of the antagonistic bacterium has technological innovation. The method can truly reflect the antibacterial ability of metabolites of the strain, has the advantages of good test stability, good repeatability, cheap materials, simple and convenient operation, short experimental period and so on, can realize large-scale determination of a plurality of antagonistic bacteria on a plurality of the pathogenic fungi, and has good function on research of the biological and ecological stability of the antagonistic bacteria.

Description

technical field [0001] The technical field of the invention belongs to the category of agronomy, the second-level discipline of plant pathology under the first-level discipline of plant protection, and more specifically relates to a method for measuring the biological activity of antagonistic bacteria necessary for the research and development of biological pesticides. Background technique [0002] 1. Biological control is playing an increasingly important role in the development of sustainable agriculture. An important part of biological control is the use of microorganisms to prevent and control diseases. Among the beneficial microorganisms, bacteria are more used to study and prevent plant fungal diseases, such as some species of Bacillus sp and Pseudomonas sp. People usually call them antagonistic bacteria or biocontrol bacteria. Many studies have proved that some bacteria have a strong inhibitory effect on the growth of plant pathogenic fungi. Silk digestion and intrac...

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to provide an improved antagonistic bacteria bioassay method to solve and overcome the deficiencies in other methods of antagonistic bacteria bioassays, such as complicated operation, time-consuming and money-consuming, poor test authenticity, stability, repeatability, and accuracy According to the characteristics of antagonistic bacteria and their metabolic secretion products, a new bioassay method for antagonistic bacteria is provided, which is simple, fast, cheap in material, convenient in source, short in experiment period, good in test authenticity, stability and repeatability. ——Double-layer culture medium filter paper sandwich method can achieve the purpose of truly and reasonably evaluating the biocontrol effect of antagonistic bacteria. Bacteria, corn sheath blight, peanut solanacear, citrus canker, etc.) for biological activity determination, also has a good effect on the biocontrol stability of antagonistic bacterial metabolic secretion products (storage at room temperature, ultraviolet irradiation)

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