Judgment method for quickly distinguishing two similar medicinal materials
A kind of medicinal material and rapid technology, applied in the direction of material separation, material analysis, measuring device, etc., can solve the problems of incompatibilities, indistinguishable forms, unstable efficacy of medicines, etc., and achieve the effect of ensuring correctness and simple method
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[0018] A method for quickly distinguishing two similar medicinal materials, comprising the following steps:
[0019] (1) Precisely draw 4.0 μl, 8.0 μl, 12.0 μl, 16.0 μl, and 20.0 μl of 0.232 mg / ml swertiamarin standard solution, and then inject samples respectively, using methanol-0.02% phosphoric acid solution as the The mobile phase is a chromatographic column with octadecylsilane bonded silica gel as a filler, and the analysis is carried out under the conditions that the detection wavelength is 240~260nm, and the number of theoretical plates is not less than 3000 based on the peak of swertiamarin, and the determination Integrate the peak area, and finally take the integral value of the peak area as the ordinate, and the swertiamarin content as the abscissa, and draw a standard curve (see figure 1 ), and calculated the regression equation of swertiamarin Y=96470.4x+226293.15, r=0.9998, indicating that swertiamarin has a good linear relationship in the range of 0.93μg~4.64μg...
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