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Thin layer identification method of alpine gentian herb and various varieties

A thin-layer identification, white flower gentian technology, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of lack of internal quality evaluation and complex varieties

Inactive Publication Date: 2015-11-25
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As far as the current situation of use is concerned, the original varieties of "Bangjian" are complex, and "Bangjian Gabao" is used in more than one kind, and there are cases where "Bangjian Enbao" is used ins

Method used

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  • Thin layer identification method of alpine gentian herb and various varieties
  • Thin layer identification method of alpine gentian herb and various varieties
  • Thin layer identification method of alpine gentian herb and various varieties

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Embodiment 1 TLC identification method (254nm) of the present invention

[0063] (1) Preparation of the test solution: take 0.5 g of the test powder, add 25 mL of methanol, sonicate for 30 min, filter, and take the subsequent filtrate to obtain the product. As the test solution.

[0064] (2) Chromatography need testing solution: take need testing solution, after pointing sample on TLC plate, carry out TLC with ethyl formate-methanol-water-formic acid=10:1:1:0.5 as developing solvent After the chromatography is completed, put the thin-layer plate under an ultraviolet lamp or / and color development for inspection.

[0065] When using the above method for identification, any one or more of gentiopicroside, swertiamarin, isoorientin, and isovitexin can be used as a control, and according to whether there is any The corresponding spots of the reference substance are used to identify the various varieties of Gentiana gentiana and their counterfeit products.

[0066] The spe...

Embodiment 2

[0087] Embodiment 2 TLC identification method (365nm) of the present invention

[0088] (1) Preparation of the test solution: take 0.5 g of the test powder, add 25 mL of methanol, sonicate for 30 min, filter, and take the subsequent filtrate to obtain the product. As the test solution.

[0089] (2) Chromatography need testing solution: take need testing solution, after pointing sample on TLC plate, carry out TLC with ethyl formate-methanol-water-formic acid=10:1:1:0.5 as developing solvent After the chromatography is completed, put the thin-layer plate under an ultraviolet lamp or / and color development for inspection.

[0090] When using the above method for identification, any one or more of gentiopicroside, swertiamarin, isoorientin, and isovitexin can be used as a control, and according to whether there is any The corresponding spots of the reference substance are used to identify the various varieties of Gentiana gentiana and their counterfeit products.

[0091] The spe...

Embodiment 3

[0112] Embodiment 3 The screening of need testing solution preparation method of the present invention

[0113] Taking samples DH-14 and MX-6 as test samples, the two extraction methods of reflux and ultrasonic were compared, and the results showed that different extraction methods could obtain clearer spots.

[0114] Compare 75% methanol, ethanol, methyl alcohol, ethyl acetate different solvents simultaneously as extraction solvent, method is as follows:

[0115] Take a total of 4 parts of 0.5 g sample powder and weigh them. Add 25 mL of 75% methanol, methanol, 95% ethanol, and ethyl acetate respectively, and weigh. Ultrasonic extraction for 30 minutes, let cool, filter, and take the filtrate to obtain the final product.

[0116] Fixing conditions: polyamide plate; sample volume: 4 μL; develop at 35°C; color development inspection method: color reagent: AlCl 3 , dried, and observed under 365nm ultraviolet light.

[0117] The screening results of the extraction solvent wer...

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Abstract

The invention provides a thin layer identification method of alpine gentian herb and various varieties; the method includes the following operation steps: (1) taking a to-be-detected medicinal material, extracting with ethanol or methanol, and preparing a test sample solution; (2) taking the test sample solution, dotting the sample on a thin layer plate, carrying out thin-layer chromatography with ethyl formate-methanol-water-formic acid with the ratio of 10:1:1:0.5 as a developing solvent, after completion of chromatography, placing the thin layer plate under an ultraviolet lamp, and/or after color developing, carrying out visual inspection. The identification method can distinguish a plurality of different-original varieties of alpine gentian herb from confusion or counterfeit species, and provides a guarantee for the quality of the alpine gentian herb medicinal material.

Description

technical field [0001] The invention relates to a thin-layer identification method of Gentiana candidum. Background technique [0002] (bāngjiān, "Bangjian"), "Bang" means "alpine meadow" in Tibetan, and "jian" means "decoration", that is, "the embellishment of alpine meadow". The Chinese name is gentian flower. "Jingzhu Materia Medica" is classified as flower medicine. According to different flower colors, it is often divided into three types: white, blue, and black. They are respectively "Bangjian Gabao" (Chinese name, white flower gentian), which has white flowers. It grows in cold areas of high mountains in late autumn, and its leaves are like Gentiana chinensis leaves. ; Stemless, four or five white flowers bloom from the ground, with red luster, and the bases of the flowers are conjoined. "Bangjian Enbao" (Blue Flower Gentian), with blue flowers, grows in very humid swamp grassland in early autumn, with small leaves and light blue flowers; "Bangjian Nabao" (Black F...

Claims

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Application Information

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IPC IPC(8): G01N30/90
Inventor 马逾英马羚古锐张彩虹
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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