10 results about "Amyrin" patented technology

This invention discloses a composition and method comprising bis(demethoxycurcumin) enriched in at least 20% w / w for inhibiting receptor for advanced glycation end products (RAGE) expression in subjects with chronic inflammatory conditions. The composition further comprises β-amyrin palmitate (BAP). This invention also discloses the use of the above composition in controlling chronic inflammatory conditions in subjects.

The invention relates to the field of bioengineering and technology, in particular to an engineering bacterium for synthesizing isoglycyrrhetinic acid, a construction method of the engineering bacterium and a method for synthesizing the isoglycyrrhetinic acid by using the engineering bacterium. The engineering bacterium comprises introduced exogenous genes, the exogenous genes comprise an HMG-CoA reductase gene, a beta-amyrin synthase gene, a beta-amyrin C-11 oxidase coding gene, a P450 reductase gene, a 11-O-beta-amyrin C-29 oxidase coding gene and an expression-enhanced endogenous gene, and the expression-enhanced endogenous gene is a beta-amyrin synthase gene, a beta-amyrin C-11 oxidase coding gene and a P450 reductase gene. Wherein the endogenous genes comprise squalene oxidase coding genes, the endogenous genes GAL80, HO and BTS1 in the engineering bacteria are knocked out, and the starting strain of the engineering bacteria is saccharomyces cerevisiae. According to the engineering bacterium provided by the embodiment of the invention, isoglycyrrhetinic acid can be obtained in an efficient, green and sustainable production mode.

This invention discloses a method for improving the yield of D-amyglucosinolates in peach kernels, belonging to the field of natural product extraction technology. Existing stepwise enzyme inactivation and defatting processes suffer from drawbacks such as cumbersome procedures and the easy isomerization of D-amyglucosinolates to L-amyglucosinolates. This invention involves pressing peach kernels at 100–150°C, completing enzyme inactivation and defatting in a single step. The resulting residue is then extracted with a 60–70 vt% ethanol aqueous solution at 70–90°C for 0.2–1 hour, repeated 1–3 times. This method overcomes the technical bias of high-temperature-induced D-amyglucosinolate isomerization, achieving a synergistic effect through the instantaneous coupling of high temperature and pressing. The extraction transfer rate of D-amyglucosinolate is no less than 81%, and the extraction transfer rate of L-amyglucosinolate is no more than 10%, with a D / L ratio greater than 15:1. This simplifies the process while achieving highly selective extraction of D-amyglucosinolates.

The invention discloses an active composition for treating atopic dermatitis and application thereof, and belongs to the technical field of biological medicine. The (+)-catechin hydrate, the epicatechin, the chlorogenic acid and the amyrin in a specific proportion are compounded to serve as active ingredients, the multi-target and multi-channel synergistic interaction effect is achieved, and the effect of remarkably treating atopic dermatitis is achieved; besides, the components of the carrier are optimized, the stability, skin permeability and slow release performance of the active components are remarkably improved, percutaneous penetration and target site accumulation of the active components are enhanced, and therefore the local drug concentration and the curative effect durability are improved; the composition has the advantages that the composition can be rapidly formed at low temperature, the biological activity of the active ingredients is better, the composition can be processed and prepared into various local external dosage forms such as gel, cream, dressing and spray according to clinical requirements, the application is flexible, the adverse reaction possibly caused by long-term use of traditional hormone or chemical synthetic drugs is avoided, and the composition has a good industrial conversion prospect.

This invention provides a CYP716 gene, protein, and applications for catalyzing the oxidation of β-amyrin at the C22α and C28 positions, belonging to the field of biotechnology. The CYP716 gene includes either CYP716BX1 (nucleotide sequence as shown in SEQ ID No. 1) or CYP716BX2 (nucleotide sequence as shown in SEQ ID No. 2). Studies have shown that CYP716BX1 and CYP716BX2 are isoenzymes, both capable of catalyzing the oxidation of β-amyrin at the C22α and C28 positions to 22α-hydroxy-kapogonadiol or 22α-hydroxy-oleanolic acid, and also capable of catalyzing the oxidation of 21β-hydroxy-β-amyrin at the C22α and C28 positions to 21β,22α-dihydroxy-kapogonadiol. This invention can provide key gene elements for the synthetic biology research of cyrimyl alcohol-type triterpenoids.

The invention discloses a pharmaceutical composition for treating and / or preventing depression, and belongs to the technical field of biological medicines. The technical problem to be solved is to significantly improve depression-like behaviors and protect nerve cells, and has an obvious effect on treatment and / or prevention of depression. According to the key point of the technical scheme, the pharmaceutical composition for treating and / or preventing depression comprises a secoiridoid glycoside compound, a xanthone glycoside compound and a beta-amyrin type pentacyclic triterpenoid compound in a mass ratio of 1: (0.25-6): (0.1-5), and one or more medically acceptable auxiliary materials.

The invention belongs to the technical field of edible oil processing, and particularly relates to camellia oleifera seed oil with high content of beta-amyrin and a preparation method thereof, and the preparation method comprises the following steps: 1) freshly squeezing camellia oleifera seed crude oil from fresh fruits; (2) preparing the camellia oleifera shell biochar; (3) co-doping calcium and phosphorus with the camellia oleifera shell biochar; 4) adsorption treatment; 5) washing and drying; 6) decoloring; (7) deodorizing; and 8) filtering to obtain the camellia oleosa seed oil with high content of beta-amyrin. The method is simple in process, the acid value and the phospholipid content of refined oil can be effectively reduced, the refining time is shortened, the refining cost is reduced, the quality stability of a camellia oleosa seed oil product is ensured, meanwhile, the content of beta-amyrin in the camellia oleosa seed oil can be effectively increased, and therefore the oxidation resistance of the finished camellia oleosa seed oil is improved.

The invention provides a conyza blinii extract (tablet) fingerprint spectrum detection method, which comprises: A) taking a test sample raw material, and pre-treating to obtain a liquid to be detected; the pretreatment mode comprises solvent dissolution and extraction; b) measuring the liquid to be measured by adopting a high performance liquid chromatography-evaporative light scattering method to obtain an HPLC-ELSD (High Performance Liquid Chromatography-Evaporative Light Scattering Detector) fingerprint spectrum of the Conyza blinii extract (tablet); chromatographic conditions of the high performance liquid chromatography are as follows: a chromatographic column is a C18 column; a mobile phase A is an acetonitrile solution, a mobile phase B is a 0.1% formic acid aqueous solution, and gradient elution is carried out; according to the method, a high performance liquid chromatography-evaporative light scattering method is adopted, an acetonitrile-0. 1% formic acid aqueous solution is selected as a mobile phase for gradient elution, ursolic acid, oleanolic acid, beta-amyrin, alpha-amyrin and friedelin are taken as reference substances, and the conyza blinii extract (tablet) fingerprint detection method is established. The scientificity and the internal quality of the conyza blinii extract (tablet) are integrally evaluated.