43 results about "Amyrin" patented technology

The invention belongs to the field of biochemical engineering and particularly relates to a building method of saccharomyces cerevisiae engineered strains capable of synthesizing glycyrrhetinic acid. The building method includes: beta-amyrin synthase gene GgbAS from Glycyrrhiza glabra, cytochrome P450 oxidases CYP88D6 and CYP72A154, cytochrome P450 oxidoreductases CPR1 and CPR2 from Arabidopsis thaliana are used to build gene expression cassettes, the gene expression cassettes are co-transformed in saccharomyces cerevisiae CEN.PK2-1C, and the saccharomyces cerevisiae engineered strains with complete glycyrrhetinic acid biosynthetic pathways are assembled and formed by the homologous recombination ability of the saccharomyces cerevisiae. By the method, the complete glycyrrhetinic acid biosynthetic pathways are introduced in the saccharomyces cerevisiae engineered strains, the glycyrrhetinic acid can be produced through fermentation, and artificial synthesizing of the glycyrrhetinic acid in the saccharomyces cerevisiae is achieved.

The invention belongs to natural products separating and extracting technical field, particularly relating to a process for simultaneously extracting essential oil, flavone and triterpene out of wrinkled giant hyssop. The invention provides a novel separating and extracting process, which can simultaneously separate and extract essential oil, flavone and triterpene by taking wrinkled giant hyssop as raw materials; the used raw materials can be wrinkled giant hyssop as well as cablin pacholi, fresh or dried herbs as well as fresh or dried leaves, stems or other parts. When fresh wrinkled giant hyssop leaves are used as the raw materials, the essential oil thereof comprises more than 40 volatile components such as cis form - isomenthone, anti form - isomenthone piperitone, hexenoic aldehyde, delta-cadinene, cadinol, alpha-muurolene, limonene and pulegone, wherein main volatile components are the cis form - isomenthone and the anti form- isomenthone, contents of which are 34.11 percent and 15.42 percent respectively; the total flavone comprises flavonoids such as acacia substance, tilianin, linarin, agastachoside, isoagastachoside, agastachin, apigenin, apigenin-7- glucoside, 6-methoxyl group apigenin, luteolin and luteolin-7- glucoside, etc., wherein main flavone components are the agastachoside and the isoagastachoside , contents of which are 25.8 percent and 8.21 percent respectively; the triterpene materials comprises triterpene compounds such as cralaegolic acid, oleanolic acid, 3-acetyl oleanolic aldehvde, 3- acetyl oleanolic acid, alpha-amyrin, beta- amyrin, campesterol, campestanol, ursolic acid, erythrodiol, erythrodiol-3-acetic ester, wherein the main triterpene component is the cralaegolic acid , the content of which is 34.6 percent. The components have wide biological activities and a plurality of effects such as liver protection, anti-tumor, anti-HIV-1, anti-proteinase activity of HIV-1, anti-herpes zoster virus and immunity improvement, etc.

The invention, which belongs to the field of preparation methods of ginseng extracts, provides a preparation method of unsaponifiable matters in ginseng seed oil. The method comprises the following steps: processing dried ginseng seeds into powder, adding petroleum ether, performing extracting by using a reflux method, and pouring out a petroleum ether solution after extraction finishing; adding sodium hydroxide and an ethanol solution into the petroleum ether solution, and performing stirring and heating; performing standing after heating finishing, taking a petroleum ether layer, and performing washing with sodium hydroxide and an ethanol solution; taking a petroleum ether layer, and performing washing with an ethanol solution; taking the petroleum ether layer, and performing concentrating under a reduced pressure or normal pressure to obtain an unsaponifiable matter in ginseng seed oil. The preparation efficiency of unsaponifiable matters in grease is improved by 20-50%, and the consumption of petroleum ether is reduced by about 50%. Gas chromatography and mass spectrometry technologies are used for detection; and the unsaponifiable matters mainly contain squalene, beta-sitosterol and beta-amyrin, and a normalization method is used for calculating that the three matters account for over 60% of the total unsaponifiable matter.

The invention discloses an amyrin cinnamate extract and a preparation method thereof. The amyrin cinnamate extract is prepared from gymnema sylvestre fruit. The preparation method of the amyrin cinnamate extract comprises the following specific steps of (1) preparation of extract; (2) preparation of extract in all positions; (3) obtaining of the amyrin cinnamate extract: taking the extract in the position of petroleum ether, separating by using silica gel column chromatography, carrying out gradient elution by using petroleum ether-ethyl acetate as an eluting agent, obtaining a yellow oily matter in a 40 to 1 eluting part, and at least repeatedly carrying out four times of recrystallization by using acetone after washing off the yellow oily matter from a solution by using the petroleum ether, thus obtaining white feather-like crystals, i.e., a compound-the amyrin cinnamate extract. According to the amyrin cinnamate extract and the preparation method of the amyrin cinnamate extract, disclosed by the invention, the ingredient of amyrin cinnamate is extracted from the gymnema sylvestre fruit for the first time, the preparation method is simple and easy to operate, and the obtained total extract yield is up to 12.18 percent to 12.93 percent.

This invention relates to isolated polynucleotides encoding enzymes consisting of a carboxypeptidase-like protein, a methyltransferase and a glucosyltransferase, involved in the biosynthesis of β-amyrin-derived triterpenes in plants and seeds. The invention also relates to the construction of recombinant DNA constructs comprising all or a portion of the isolated polynucleotides of the invention, in sense or antisense orientation, operably linked to at least one regulatory sequence.

The invention discloses a yarrowia lipolytica engineering strain for biologically synthesizing glycyrrhetinic acid by taking glucose as a substrate as well as construction and application thereof, and belongs to the technical field of biology. According to the engineering strain, a beta-amyrin synthase expression module, a 11 / 30 site-oxidized beta-amyrin synthase expression module, a cell P450 oxidase expression module and a cytochrome P450 reductase expression module are integrated on a genome; the construction method comprises the following steps: constructing to obtain glycyrrhetinic acid biosynthesis module plasmids: beta-amyrin synthase bAS, cytochrome P450 oxidase 88D6CYP88D6, 11 / 30-site-oxidized beta-amyrin synthase CYP725A4 and cytochrome P450 reductase CPR, integrating the glycyrrhetinic acid biosynthesis module onto a yarrowia lipolytica tool plasmid, and linearizing the plasmids integrated with the glycyrrhetinic acid biosynthesis module, and introducing the linearized plasmids into yarrowia lipolytica to obtain the yarrowia lipolytica engineering bacteria of which the genome is integrated with the glycyrrhetinic acid biosynthesis module. The yield of glycyrrhetinic acid produced by the engineering strain is remarkably improved and reaches 7g / L to the maximum.

The invention discloses compositions and methods comprising enriched Bisdemethoxycurcumin (BDMC) present not less than 20% w / w for use in inhibiting Receptor for Advanced Glycation End-Products (RAGE) expression in a subject with chronic-inflammatory condition. The composition further comprises β-amyrin palmitate (BAP). The invention also includes disclose the use of the above composition in the management of chronic inflammatory condition in a subject

β-Amyrin, a precursor in biosynthesis of soyasapogenol B, is biosynthesized by cyclization of 2,3-oxidosqualene which is generated by the mevalonate pathway, and soyasapogenol B is biosynthesized by two hydroxylations of β-amyrin. However, a gene of 22-hydroxylase involved in the sequence of reactions has not been identified. The present inventors identified a gene encoding the hydroxylase for oleanene triterpenes at C-22, and found that oleanene triterpenes could be hydroxylated at C-22 by co-expressing this gene together with one or more specific genes. Further, the present inventors found that soyasapogenol B could be efficiently produced in large quantities by co-expressing this gene for 22-hydroxylase with a gene for 24-hydroxylase.