Tripterygium wilfordii triterpene synthase twosc3 and its coding gene and application

A coding and amino acid technology, applied in application, genetic engineering, plant genetic improvement, etc., can solve the problems of restricted development, slow growth of plants, and low content.
CN108866029BActive Publication Date: 2019-06-04CAPITAL UNIVERSITY OF MEDICAL SCIENCES
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CAPITAL UNIVERSITY OF MEDICAL SCIENCES
Publication Date
2019-06-04

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Abstract

The invention relates to a triterpene synthase of tripterygium wilfordii TwOSC3 protein and an encoding gene thereof. A recombinant expression vector with a Twosc3 gene is constructed by cloning cDNAof the Twosc3 gene onto a eukaryotic expression vector pYES2, and then the recombinant expression vector is shifted to yeast expression host bacteria, so as to acquire friedelin. A mutation research result shows that amino acid 482 encoded by the Twosc3 gene is a key site, and the yield of friedelin or amyrin can be increased or reduced; a gene gun mediated interference experiment shows that the interference of the Twosc3 gene has an obvious inhibiting effect on the synthesis of celastrol in tripterygium wilfordii; the TwOSC3 protein and the encoding gene thereof provided by the invention canbe used for biosynthesizing plant triterpenoids and cultivating high-quality tripterygium wilfordii.
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Description

technical field

[0001] The invention relates to tripterygium wilfordii triterpene synthase and its encoding gene, and the application of the triterpene synthase and its encoding gene in the biosynthesis of triterpenoids, belonging to the field of medicinal plant genetic engineering. Background technique

[0002] The traditional Chinese medicine Tripterygium wilfordii, derived from the xylem of the dried root of the medicinal plant Tripterygium wilfordii. Hook.f., is widely used in the treatment of rheumatoid arthritis and inflammation (Raphaela G M, Mildred W, Roy F, et al .Comparison of Tripterygium wilfordii Hook F Versus Sulfasalazine in the Treatment of Rheumatoid Arthritis:A Randomized Trial[J].Annals of Internal Medicine,2009,151(4):229-240.Tao X L,Lipsky P E.The Chinese anti-inflammatory and immunosuppressive herbal remedy Tripterygium wilfordii Hook F. [J]. Rheumatic Disease Clinics of North America, 2000, 26(1):29–50.). Terpenes are the main active ingredients of T...

Claims

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