16 results about "Multifunctional Enzymes" patented technology

The invention discloses an enzyme-inspired hydrogen bond organic framework material as well as a preparation method and application thereof. The enzyme-inspired hydrogen bond organic framework material is formed by self-assembling Py-4DAT monomer molecules through hydrogen bonds. The multifunctional enzyme inspired hydrogen bond organic framework material is synthesized through bionic design, efficient capture, rapid detection and self-driven degradation of a nerve poison simulant DCP are achieved, and the bottleneck of a traditional porous material in the aspects of selective recognition and multifunctional synergy is broken through; the FDU-HOF-5 material has high stability, solution processability and real-time response capability, a functional textile prepared by loading the FDU-HOF-5 material can realize instant visual detection of DCP, a new material and a new strategy are provided for intelligence of chemical protection equipment, and the FDU-HOF-5 material has important application value in the field of chemical threat prevention and control.

1. The name of the design product: multifunctional enzyme label instrument. 2. The use of the design product: for nucleic acid, protein concentration, enzyme activity analysis and other conventional molecular detection; signal transduction research, cell signal event active oxygen, modification detection; cell viability, apoptosis, killing and other overall level analysis. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective drawing.

The invention discloses a high-flux toxicity pre-evaluation substitution method based on an acetylcholin esterase target. By integrating an SH-SY5Y nerve cell endogenous AChE and recombinant exogenous AChE dual detection system and relying on an automatic screening system constructed by integrating core equipment such as an automatic pipetting workstation, a constant-temperature incubator and a multifunctional microplate reader, a high-flux toxicity pre-evaluation substitution method based on an AChE target is successfully established and is used for evaluating the neurotoxicity of a compound, and the method has the advantages of high sensitivity, high sensitivity, high sensitivity and the like. The method solves the limitation that a single screening method in the prior art cannot evaluate factors such as target inhibition, cell permeability and metabolic transformation at the same time, and the method is simple, convenient, rapid, high in accuracy and suitable for large-scale primary screening of the AChE inhibitor and early warning of neurotoxicity risks.

This invention relates to the field of enzyme network analysis technology, specifically a robust system for analyzing the functional balance of a multifunctional enzyme regulatory network. The system includes a network topology analysis module, a graph generation module, a flux balance analysis module, a functional simulation module, a robustness curve fitting module, and a report generation module. It identifies key nodes and vulnerable paths in the target regulatory network; constructs a structural feature association graph; performs flux balance analysis on the target regulatory network to obtain functional flux distribution data; simulates the functional operation of the target regulatory network and quantifies the output efficiency of the simulation results to obtain the core function maintenance degree; fits the perturbation trend of the core function maintenance degree to obtain a functional balance robustness curve; and analyzes the decay mode of the functional balance robustness curve to generate a robustness analysis report of the target regulatory network. This invention can improve the efficiency of functional balance robustness analysis of a multifunctional enzyme regulatory network.

This application relates to the technical fields of synthetic biology and enzyme engineering, and in particular to a computational-experimental closed-loop rational design method for polyketide synthases. The method includes: constructing a full-length FCHS model using AlphaFold3; identifying binding hotspots and verifying dynamic stability through molecular docking and kinetic simulations; and constructing a low-redundancy mutant library containing A, L, and F substitutions. This application solves the problems of difficult protein structure analysis and limited experimental throughput through a closed-loop process of computational pre-screening, dynamic verification, and evolutionary filtering, providing an efficient paradigm for the improvement of complex multifunctional enzymes.

The application discloses a pseudomonas aeruginosa bacteriophage PAZ1 and application thereof, the bacteriophage PAZ1 has a preservation number of CCTCC NO:M 2024545; the application utilizes the bacteriophage to prepare a detection kit, the kit can effectively detect the pseudomonas aeruginosa in food samples, is coupled with nano magnetic beads to form a conjugate PhagePAZ1-MBs.The pseudomonas aeruginosa after enrichment and separation is lysed by CTAB, is reacted with a luminescent reagent and is read by a multifunctional enzyme label instrument, the number of the pseudomonas aeruginosa in the sample is determined, and the detection specificity of the method is good.The application utilizes the pseudomonas aeruginosa bacteriophage fluorescent detection kit to qualitatively and quantitatively detect the sample to be measured, the detection time is about 30 min, and the detection limit reaches 607 CFU / mL.The application expands the application range of the bacteriophage in water source food safety detection, and has a good application prospect.

The multifunctional enzymolysis equipment comprises an enzymolysis tank, a heat exchange jacket, a stirring device, a speed reducer and a control system, the top of the enzymolysis tank is provided with a feed port and a PH meter port, a flow meter can be mounted at the feed port, and a PH meter can be mounted at the PH meter port; a first discharge hole and a pressure manhole are formed in the bottom of the enzymolysis tank, and a pressure gauge can be mounted in the pressure manhole; a thermometer port and a sampling port are formed in the side of the enzymolysis tank, and a thermometer can be mounted at the thermometer port; the heat exchange jacket comprises a heat exchange pipeline surrounding the outer wall of the enzymolysis tank body, and a steam inlet and a condensed water outlet which are respectively communicated with the heat exchange pipeline; and the speed reducer is connected with a suspended stirring shaft of the stirring device. And the control system is connected with the flow meter, the PH meter, the pressure gauge, the thermometer and the speed reducer. According to the utility model, differences caused by manual operation can be eliminated, and the enzymolysis process can be monitored and controlled, so that the production efficiency and the product quality are improved, the cost is reduced, and the utilization rate of raw materials and energy is improved.

The utility model relates to the technical field of anti-aging raw material extraction, in particular to multifunctional enzymolysis extraction equipment which comprises a box body and a connecting unit, the connecting unit comprises an inner shell, a connecting bin, a discharging pipe, an electromagnetic valve, a temperature sensor, an electric heating wire, a mounting seat, a side door, a controller, a stirring assembly and a separating assembly, and the temperature sensor is fixedly connected with the box body; during use, a backing material and enzyme liquid are placed in the inner shell, then the side door is closed, the controller is clicked to start the electric heating wire, the temperature is increased by utilizing heat conductivity, the temperature sensor transmits the temperature in the box body to the controller in real time, the stirring assembly is connected with the stirring assembly, and the separation assembly is arranged below the discharging pipe. After the processing is completed, the separation assembly is used for filtering, and the stirring assembly is used for stirring and mixing the base material and the enzyme liquid, so that the efficiency of extracting the anti-aging raw material is improved, the complex technological process is shortened, and the workload of workers is effectively reduced.

The invention discloses a flavin-dependent oxidase SavA and an application of the flavin-dependent oxidase SavA in asymmetric synthesis of 4-alkyl butenolactone. The SavA enzyme provided by the invention is a novel multifunctional enzyme with desaturation, hydroxylation and lactonization effects at the same time, and can catalyze conversion of fatty acyl thioester into single-configuration (4S)-4-alkyl butene lactone. The SavA enzyme provided by the invention is a flavin-dependent enzyme and naturally exists in a form of being combined with FAD, no exogenous FAD needs to be added in an in-vitro enzymatic reaction, no other coenzyme or cofactor is involved, only O2 is used as an oxidizing agent, the reaction process is green and efficient, and the SavA enzyme has the potential of being developed into an efficient tool enzyme for asymmetric biological catalytic synthesis of 4-substituted butenolactone.

The invention relates to the technical field of biology, in particular to a cellulase technology in the field of enzymology. The invention relates to the technical field of cellulose degradation, in particular to application of a GH5GH6 double-catalytic structural domain compound enzyme (containing a glycoside hydrolase family 5 (GH5) and a glycoside hydrolase family 6 (GH6)) in cellulose degradation. The invention relates to an application of a GH5GH6 double-catalytic structural domain compound enzyme in cellulose degradation. The GH5GH6 double-catalytic structural domain compound enzyme is derived from ship maggot symbiotic bacteria. The ship maggot symbiotic bacterium is a known strain Teredinibacter species, in particular to a Teredinibacter Turnerae strain, and the ship maggot symbiotic bacterium can be used for preparing the ship maggot symbiotic bacterium. According to the invention, a multifunctional enzyme compound catalyzed by double structural domains of GH5 and GH6 is obtained from a ship maggot symbiotic bacterium Teredinibacter, the compound is formed by arranging and connecting the GH5, the GH6 and 2-4 CBMs, three different functional structural domains of the GH5, the CBMs and the GH6 are integrated, and the efficiency and the stability of cellulose degradation are remarkably improved.

The invention discloses a molecular marker of a wheat grain storage protein regulatory gene TaMYB3R1-3D and application of the molecular marker, and belongs to the technical field of biology. The molecular marker is a group of KASP primers and comprises two reverse primers as shown in SEQ ID NO: 1 and SEQ ID NO: 2 (5'ends are respectively marked with recognition sequences of FAM and HEX fluorescent probes) and a forward primer as shown in SEQ ID NO: 3, a PHERAstar Plus multifunctional microplate reader is used for detecting a PCR (Polymerase Chain Reaction) product, and software KlusterCaller is used for carrying out genetic typing. The molecular marker has the advantages that the content of the storage protein of the wheat can be predicted by applying the KASP molecular marker developed by the invention, the cost is saved, the selection efficiency is greatly improved, the breeding process can be accelerated, and a useful molecular tool is provided for efficiently screening high-quality wheat strains.

The invention discloses a multifunctional enzyme microorganism plant boosting agent and a preparation method thereof, and belongs to the field of agricultural water-soluble fertilizers. The boosting agent is prepared from amino acid powder, brown sugar, potassium humate / potassium fulvate, monopotassium phosphate, an enzyme microbial agent, table vinegar and medical stone mineralized water in proportion. The preparation method comprises the following steps: dissolving the brown sugar with boiled medical stone mineralized water, cooling, adding the enzyme microbial inoculum for activation, and standing; adding table vinegar and the residual medical stone mineralized water, and controlling the temperature to ferment for 90-96 hours until the main fermentation is finished; and finally, standing the zymophyte liquid, adding amino acid powder, potassium humate / potassium fulvate and monopotassium phosphate, and uniformly stirring and chelating at 40-45 DEG C to obtain the plant boosting agent. The plant boosting agent is high in activity, green and pollution-free, has the effects of supplementing nutrients, improving soil, resisting diseases and the like, is suitable for various crops, is good in greenhouse planting adaptability, is proved to be remarkable in yield increasing and quality improving effects by field experiments, and is simple and convenient in preparation process, low in cost and suitable for industrial production.

This invention discloses a method for screening drugs against Japanese encephalitis virus, which uses the main protease NS3 of Japanese encephalitis virus. pro The activity reporter plasmid and the main protease NS3 of Japanese encephalitis virus pro Expression plasmids were used to screen for drugs against Japanese encephalitis virus; NS3 pro Active reporter plasmids and NS3 pro The expression plasmid was co-transfected into HEK-293T cells. The fluorescence intensity of green fluorescent protein (EGFP) was detected using an inverted fluorescence microscope and a multi-functional microplate reader to predict NS3. pro The activity of NS3, the main protease of JEV, can be used to screen for its activity. pro The purpose of this invention is to screen anti-JEV compounds using active reporter plasmids. The inhibitory effect of the compounds can be observed more intuitively and easily by utilizing fluorescence intensity, providing a powerful tool for screening broad-spectrum anti-JEV compounds.

This invention belongs to the field of biomedical engineering technology, specifically relating to the construction and application of a screening model for influenza virus receptor binding inhibitors. Addressing the problem of influenza virus hemagglutinin (HA) being prone to mutation and having multiple subtypes, making it difficult to screen for broad-spectrum inhibitors, this invention replaces HA with elderberry lectin (SNA), which specifically recognizes α-2,6-sialic acid. Using MDCK cells overexpressing α-2,6-sialic acid, a fluorescently labeled high-throughput screening model is constructed by labeling SNA with fluorescein isothiocyanate (FITC). The relative fluorescence units (RFU) of the system are detected using a multifunctional enzyme-linked immunosorbent assay (ELISA) reader. Active compounds inhibit the binding of SNA to cell surface receptors, resulting in a lower RFU value, while inactive compounds show a higher RFU value. This invention provides key technical support for the development of novel inhibitors targeting influenza virus receptor binding.

A systematic pipeline is used to extract catalytically active pockets of the most diverse enzyme class—hydrolases, from the PDB database. A process extracts the 38029 hydrolase reactive centers (RC) and collates them into a publicly accessible active site collection (actiome; RC-Hydrolase). The process includes 128M pairwise shape comparisons across RC-Hydrolase using CADSEEK 3D Shape Search Engine to end up with 155,329 instances presented in a available, visually interactive dataset. Allowing comparisons of enzyme reactive centers across functional spaces (EC classification numbers) enables identification of enzyme backbones which can be minimally mutated to accommodate more than one type of catalytic activity to aid rational design of multifunctional enzymes. Such versatile enzyme backbones is leveraged by latest diffusion-based protein design models to design a library of structurally stable multifunctional enzyme pockets. Design of a bifunctional protease-nuclease shown as an example opens up a novel computational recipe for enzyme engineering.