Retinoic acid X receptor alpha activation effect molecule detection method based on fluorescence polarization technology

By combining fluorescence polarization technology with a multifunctional microplate reader to detect the RXRα activation effect, the problems of low sensitivity and high cost in the existing technology were solved, and high-throughput, low-cost screening of RXRα activators was achieved.

CN120629583APending Publication Date: 2025-09-12KUNMING UNIV OF SCI & TECH
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Patent Information

Application Number
CN202510616488.9
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-05-14
Publication Date
2025-09-12

AI Technical Summary

Technical Problem

Existing RXRα activation effect detection technology has problems such as low sensitivity, high cost and strong equipment dependence, which limits its application in scientific research and clinical treatment.

Method used

Fluorescence polarization technology combined with a multifunctional microplate reader was used to detect the fluorescence polarization signal of a mixed solution of retinoid X receptor α and fluorescent nuclear receptor coregulatory peptide, and the activation effect of the test substance on RXRα was analyzed using T-test.

Benefits of technology

The system realizes low-cost, high-throughput, simple-operation and highly sensitive detection of RXRα activation effects, is suitable for multifunctional microplate readers, and is suitable for high-throughput screening of RXRα agonists.

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Abstract

The invention discloses a retinoic acid X receptor alpha activation effect molecule detection method based on a fluorescence polarization technology, and belongs to the technical field of biological analysis. According to the method, the change of a probe fluorescence polarization signal caused by the change of the binding state of the RXR alpha and the fluorescent nuclear receptor co-regulatory peptide is directly monitored, so that the rapid evaluation of the to-be-detected substance on the receptor activation effect is realized. According to the method, three core processes of background signal determination, to-be-detected substance signal detection and statistical analysis are completed on the molecular level by virtue of a conventional multifunctional microplate reader, so that the dependence on high-cost equipment or a complex cell model in the prior art is effectively avoided, and high efficiency, low cost and high flux of RXR alpha activator screening are realized. The method is suitable for early warning of toxicity of environmental compounds and efficient screening of RXRalpha targeted drugs, and a practical tool is provided for receptor function research and drug development.
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