593 results about "Biosynthetic product" patented technology

The invention discloses a biosynthetic bacterial cellulose-based solid electrolyte as well as a preparation method and application thereof, and belongs to the technical field of solid electrolytes. The solid electrolyte is prepared by taking modified bacterial cellulose prepared by fermenting acetobacter xylinum, rhizobium, pseudomonas, azospirillum, alcaligenes and other bacteria as a substrate, introducing a low-molecular-weight polymer to regulate and control the arrangement of cellulose molecular chains to serve as a conductive ion framework, and then compounding a conductive ion high-molecular-weight polymer and a lithium salt. According to the invention, cellulose molecular chains are modified by modified glucose, and a network structure is regulated and controlled by a low-molecular-weight polymer, so that the problems of small spacing, lack of ion coordination sites and weak ion transmission capability of pure cellulose molecular chains are effectively solved, the ionic conductivity of the prepared solid electrolyte can reach 10 <-4 > S cm <-1 >, the electrochemical window width reaches 5V, and the solid electrolyte has a good application prospect. The material can tolerate high critical current density, is suitable for various positive electrode materials, and is suitable for high-voltage and long-cycle-life energy storage devices.

The application discloses a galactosyltransferase mutant and application thereof in preparation of flavonoid compounds, and belongs to the technical field of enzymology. The application provides a UDP-galactosyltransferase mutant, which is obtained by site-directed mutation of one amino acid in the amino acid sequence of wild-type galactosyltransferase VcUFGT into alanine (A). The effects of a series of mutants H82A, V139A, G141A, P186A, N245A, V282A and S307A are verified in the examples, the mutants can perform enzyme catalysis reaction with quercetin and UDP-galactoside as substrates, and generate flavonoid compound hyperoside, and the specific enzyme activity of the mutants is more than 1.5 times that of the wild type, thereby providing an effective application basis for biosynthesis of flavonoid compounds.

The invention discloses a phenanthridine-6-formaldehyde derivative and a preparation method and application thereof.The preparation method comprises the steps that 2, 4, 5, 6-tetra (9-carbazolyl)-isophthalonitrile serves as a photocatalyst, 1-azabicyclo [2.2. 2] octane serves as a hydrogen atom transfer catalyst, a biphenyl isocyanide compound, the photocatalyst, the hydrogen atom transfer catalyst and an alkali additive are jointly dissolved in 1, 3-dioxolane, the mixture is stirred to be uniform, and the phenanthridine-6-formaldehyde derivative is obtained. And carrying out free radical tandem cyclization and acid-catalyzed hydrolysis reaction to obtain the phenanthridine-6-formaldehyde derivative. The invention also provides a specific reaction equation. According to the developed synthesis method of the phenanthridine-6-formaldehyde derivative, the compound can be efficiently and conveniently prepared, the technical bottlenecks of harsh reaction conditions and tedious steps in a traditional process are successfully overcome, raw materials which are low in price and easy to obtain are adopted, operation is carried out under mild conditions, the synthesis method has the outstanding advantages of being high in yield, safe and simple to operate, environmentally friendly and the like, and the method is suitable for industrial production. Good industrial application potential is shown.

The invention relates to the technical field of enzyme engineering, in particular to an acylase mutant and a biosynthesis method of (R)-2-aminobutanol.The acylase mutant comprises at least one of mutation of the following sites relative to wild type acylase with the amino acid sequence being SEQ ID NO: 1: T106, A625 and S413. The acylase mutant disclosed by the invention is used for synthesizing (R)-2-aminobutanol through biological catalysis, and the reaction route is as follows: the catalytic efficiency of a product is remarkably improved by the mutant, and the ee value of a chiral product is improved; according to the synthesis method, the problem of chiral resolution in the prior art is solved, the production cost is reduced, and pollution is reduced.

The invention belongs to the technical field of biosynthesis, and particularly relates to an engineering bacterium for preparing stevioside through de novo fermentation as well as a preparation method and application of the engineering bacterium. According to the invention, mevalonate kinase in the MVA pathway is mutated and optimized. The method comprises the following steps: carrying out point mutation on mevalonate kinase MvK of a wild type source, constructing a stevioside synthesis route in an escherichia coli host, and comparing the influence of MvK mutation on the yield of stevioside, so as to determine a Q160L mutant, and applying the Q160L mutant to the stevioside production route to realize efficient production of stevioside.

The present invention provides a biomass gasification control system and a biomass gasification control method, and belongs to the field of biosynthesis, the biomass gasification control system comprises: a first-stage fluidized bed pyrolysis reactor for performing a preliminary pyrolysis reaction on a biomass raw material to obtain a pyrolysis reactant; the second-stage fluidized bed gasification reactor is used for carrying out gasification reaction on the pyrolysis reactant by utilizing various gasification agents to obtain target synthesis gas, including H2 and CO; wherein the multiple gasifying agents comprise oxygen, water vapor and carbon dioxide; the sensor monitoring module is used for monitoring real-time state data in the first-stage fluidized bed pyrolysis reactor and the second-stage fluidized bed gasification reactor; the central control module is used for sending out an adjusting instruction according to the real-time state data, and the adjusting instruction is used for adjusting and controlling the flow of the multiple gasifying agents, the rotating speed of the fan and the reaction temperature, so that the molar ratio of H2 to CO in the generated target synthesis gas is within a first threshold value range.

The invention discloses a 7alpha-hydroxysteroid dehydrogenase mutant with improved catalytic activity as well as a coding gene and application thereof, and the amino acid sequence of the 7alpha-hydroxysteroid dehydrogenase mutant is obtained by mutation at one or more sites of the 120th site, the 195th site and the 207th site on the basis of the amino acid sequence shown in SEQ ID NO: 1. According to the 7alpha-HSDH mutant disclosed by the invention, the key site of the 7alpha-HSDH enzyme is modified, so that the mutant with higher activity than that of a wild enzyme is obtained, the efficiency bottleneck in UDCA biosynthesis is effectively broken through, and an efficient and green enzyme catalyst is provided for industrial application.

The invention discloses a method for improving the solubility and thermal stability of sweet protein, and belongs to the technical field of biosynthesis, the method comprises the following steps: constructing a gene tandem recombinant plasmid containing a monellin x-3C-sfGFP-3C-monellin y expression cassette, x is greater than or equal to 1, y is greater than or equal to 1, 5 is greater than or equal to x + y is greater than or equal to 3, a 3C protease recognition sequence is also inserted between adjacent copies of monellin, and each monellin is connected with a purification tag; the gene tandem recombinant plasmid is transformed into escherichia coli and inducible expression is carried out, thalli are split and purified to obtain fusion protein, 3C protease is used for enzyme digestion, and the target protein is obtained after re-purification. The total protein yield and the solubility proportion are remarkably improved by adopting a series construction mode of plasmids, meanwhile, the stability of monellin is effectively improved, the purification step is simpler and more convenient, and the method is suitable for industrial production.

The invention belongs to the technical field of biosynthesis of plant secondary metabolites, and particularly relates to a key gene for biosynthesis of large-fruit hawthorn flavonoid compounds and a screening method and application of the key gene. Through combined analysis of metabolome and transcriptome, a key gene combination containing seven structural genes and three transcription factor genes is screened out; the expression of the genes is remarkably positively correlated with the accumulation of a target flavone metabolite [6]-gingerol, and the genes are core factors for regulating and controlling the synthesis of the flavonoid compounds of the big-fruit hawthorns through experimental verification. According to the invention, the key gene for regulating and controlling the synthesis of flavone substances such as [6]-gingerol in the big hawthorn fruit is systematically identified for the first time, and a target spot is provided for analyzing a quality formation mechanism from a molecular level; the gene can be used for molecular marker-assisted breeding so as to cultivate a new variety of large-fruit hawthorn with high flavone content, and also can provide gene resources and technical support for the development of functional food and health care products.

Described herein are biocatalytic methods of producing terpene degradation products useful as starting material for the production of perfumery ingredients, such as, for example, ambrox. In particular novel terpene degrading polypeptides (enal-cleaving polypeptides) and novel peptides converting terpenes compounds to oxygenated derivatives (oxygenases) and mutants and variants derived therefrom are described which may be applied in novel types of fully enzymatic multistep degradation pathways allowing the controlled, stepwise conversion and degradation of linear or cyclic terpene substrates. Said novel biosynthetic strategies allow the fully biochemical synthesis of valuable terpene-derived compounds, like for example manooloxy or gamma ambrol. Also described herein are recombinant host organisms carrying the required set of genetic information for the functional expression of the set of enzymes necessary for catalyzing the combination of enzymatic conversion and degradation steps.

The invention relates to the technical field of enzymology, in particular to a gene for biosynthesis of (Z)-9-hexadecenoic acid and application of the gene. The invention discloses a gene for biosynthesis of (Z)-9-hexadecenoic acid. A nucleotide sequence coded by the gene is shown as SEQ ID NO: 1. The gene and hexadecanoic acid specific thioesterase are jointly expressed in plants, so that the biosynthesis yield of (Z)-9-hexadecenoic acid can be increased. The process of synthesizing the (Z)-9-hexadecenoic acid by using plants is green and pollution-free, and the purification is simple and convenient, so that the environmental pollution degree is low, and the extraction purity is high, thereby changing the path depending on chemical synthesis, and solving the defects of complex chemical synthesis, large by-product amount, environmental pollution, high cost, low final product purity and the like.

The invention belongs to the technical field of microbial fermentation, and relates to bacillus cereus capable of biologically converting limonene, the bacillus cereus is preserved in the China Center for Culture Collection, the classification name is Bacillus cereus GJ-02, the preservation number is CCTCC M 20251438, and the preservation date is June 23, 2025; according to the invention, the bacillus cereus strain capable of biologically converting limonene and having efficient conversion capability is separated and screened, a new strain resource is provided for microbial conversion of D-limonene, and a theoretical basis is provided for biosynthesis and metabolic engineering modification of terpenoids; therefore, the method has a wide application prospect in the field of microbial fermentation.

The invention relates to the technical field of polypeptide biosynthesis, in particular to a method for biosynthesizing acetyl tetrapeptide-5, a composition containing the acetyl tetrapeptide-5 and application of the composition. According to the method, low-cost natural amino acid is used as a substrate, specific aminopeptidase or a mutant thereof is used as a biocatalyst, amino acid ligase and polyphosphatase are combined, and acetyl tetrapeptide-5 can be efficiently synthesized through a one-step enzymatic reaction. Compared with a traditional chemical synthesis method, the method has the advantages of low raw material cost, no use of an organic solvent, mild reaction conditions, high regioselectivity, environmental friendliness, low production cost and the like. The invention also provides a composition containing acetyl tetrapeptide-5 and dipeptide-15, the inhibition effect of the composition on grease secretion is obviously better than that of a single component, and the composition has a synergistic oil control effect under a specific ratio.

The invention belongs to the technical field of microbial fermentation, and relates to a nitrate reducing pseudomonas capable of biologically converting limonene, which is preserved in the China Center for Culture Collection, the classification name of which is Pseudomonas nitritiducens GJ-01, the preservation number of which is CCTCC M 20251439, and the preservation date of which is June 23, 2025, and the preservation number of which is CCTCC M 20251439. According to the invention, the nitrate reducing pseudomonas strain with efficient conversion capability and capable of biologically converting limonene is separated and screened, so that a new strain resource is provided for microbial conversion of D-limonene, and a theoretical basis is provided for biosynthesis and metabolic engineering modification of terpenoids; therefore, the method has a wide application prospect in the field of microbial fermentation.

The invention provides a high-temperature-resistant lipopeptide reinforced foam flooding system and a screening method and application, and relates to the technical field of microbial oil recovery, the high-temperature-resistant lipopeptide reinforced foam flooding system comprises the following steps: S1, screening applied oil reservoirs; s2, based on the oil reservoir temperature and the crude oil viscosity selected in S1, screening a high-temperature-resistant lipopeptide reinforced foam flooding system; the high-temperature-resistant lipopeptide reinforced foam oil displacement system comprises a foaming agent, a foam stabilizer, gas and water, the foaming agent comprises one or more of surfactin (C12-18), scabrin (C14-18), iturin (C14-17), deocytin, serratia marcescens and mucin fermentation liquor, and the foaming agent comprises one or more of lipopeptide, a foaming agent and a foaming agent. The foam stabilizer comprises one or more of biosynthetic nano-silver, nano-iron, nano-copper, nano-zinc, nano-zirconium and nano-nickel. The method has the beneficial effects that the oil reservoir application range is wide, the pertinence is high, the field test effect is good, and a high-temperature-resistant lipopeptide enhanced foam oil displacement system is specifically constructed according to the temperature-resistant stability characteristics of lipopeptide surfactants and biosynthetic nano materials of different structures and types.

The invention discloses a method for efficiently producing pyridoxal phosphate based on a three-enzyme cascade reaction and a recombinant strain, and belongs to the technical field of biological engineering. The recombinant escherichia coli for co-expressing the pyridoxine phosphate phosphatase mutant, the pyridoxine phosphate oxidase and the hydrogen peroxide decomposing enzyme is constructed, three-enzyme synergy is realized by optimizing the copy number of a carrier, and the catalytic efficiency of a recombinant strain as a cell catalyst is improved. The invention further provides a one-pot method for preparing pyridoxal phosphate, the production cost is reduced, an efficient, green and economical biosynthesis path is provided for industrial production of PLP, and the method can be widely applied to the fields of food, medicine and the like and has important industrial value and application prospects.

The disclosure provides compositions and methods related to engineered microbial cells, enzymes, and methods for producing dammarenediol, as well as compounds derived from dammarenediol. Microbial host cells are engineered to express a heterologous biosynthetic pathway that produces dammarenediol, or a derivative thereof. The host cell can optionally express a heterologous uridine diphosphate-dependent glycosyltransferase (UGT) enzyme producing natural or non-natural glycosylated forms of dammarenediol, protopanaxadiol or protopanaxatriol.

In various embodiments, provided herein are host cells, methods, and pharmaceutical compositions comprising gastrodin, wherein the gastrodin is produced by a genetically modified plant or plant cell, fungal cell, yeast cell, insect cell, or bacterial cell. In certain embodiments, the present disclosure provides methods and compositions for the production of gastrodin. In yet other embodiments, the present disclosure provides enhanced cells and methods for producing gastrodin.

The invention provides a benzoylurea substituted isoxazoline derivative as well as a preparation method and application thereof. The compound has the following chemical structural general formula, wherein in the chemical structural general formula CY, R is substituted aryl or aliphatic chain. In particular to a series of benzene ring, pyridine ring, benzyl or aliphatic chain substituted derivatives, a novel insecticide with high efficiency, low toxicity and broad-spectrum insecticidal performance is synthesized, the novel insecticide can be alternately used with existing insecticides, generation of resistance is avoided or delayed, preparation conditions are conventional, follow-up treatment is simple and convenient, and industrialization is easy to achieve.

The application discloses a biosynthesis method of blue copper peptide, comprising the following steps: synthesizing a recombinant protein according to an amino acid sequence shown in SEQ ID NO. 1, synthesizing a gene coding the recombinant protein, constructing a recombinant plasmid by using the gene, transferring the recombinant plasmid into a host bacterium, carrying out fermentation culture to induce protein expression, separating the protein, carrying out enzyme cutting and purification, and finally carrying out complexing with copper ions to obtain the blue copper peptide. Compared with the prior art, the biosynthesis method of the blue copper peptide has the advantages of low production cost, green environmental protection and high yield by optimizing the design of the fusion protein, and the yield of the final product can be significantly improved, and the fusion protein has better structure and isoelectric point characteristics.

The present invention relates to a novel strain having an excellent polyhydroxyalkanoate-producing ability and a use thereof. A species of Halomonas ixanhensis, which previously has not been reported to have polyhydroxyalkanoate biosynthesis characteristics, was found to produce polyhydroxyalkanoate. A novel HN-1-3-2 strain that produces polyhydroxyalkanoate in a significantly higher content and concentration than existing polyhydroxyalkanoate-producing strains has been selected, and thus can be used for the purpose of producing polyhydroxyalkanoate.

The invention discloses klebsiella pneumoniae and application thereof in production of indole-3-carboxylic acid (I3CA), and belongs to the fields of microbial technology, biological catalysis and fermentation engineering. The preservation number of the klebsiella pneumoniae is CCTCC NO: M 20252762, the classification name of the klebsiella pneumoniae is Klebsiella pneumoniae I3CA-02, the strain can efficiently convert tryptophan into I3CA in a time-dependent mode through a one-step method, high sensitivity to various clinically common antibiotics is kept, and the klebsiella pneumoniae has good intestinal colonization ability and safety, and can be used for preparing I3CA. The method has the potential of being directly applied to oral micro-ecological preparations and industrial production. The technical bottlenecks that the existing I3CA biosynthesis efficiency is low and the safety of recombinant bacteria is limited are broken through, the microorganism sources of indole derivatives are widened, and a foundation is laid for green manufacturing of high-purity I3CA and development of intestinal-brain-immune axis related functional products.

The invention relates to a pyridoxal phosphate (PLP) synthesis method based on cascade biological catalysis and capable of realizing ATP autonomous regeneration. The method comprises the following steps: constructing engineering bacteria for co-expressing pyridoxal phosphate kinase (PdxK) and polyphosphate kinase (PPK) to prepare a whole-cell catalyst or a crude enzyme solution; under the condition that ATP does not need to be exogenously added, pyridoxal, ADP and polyphosphate serve as substrates, cascade reaction is carried out under the action of a catalyst, ATP is regenerated in situ, and PLP synthesis is driven. A wild enzyme system is adopted, and the yield of PLP exceeds 95% within 4 hours of reaction; after PdxK mutation optimization, the reaction time is shortened to about 2 hours, and conversion can be completed. According to the invention, a polyphosphate-driven ATP regeneration system is coupled with PLP biosynthesis, so that the cofactor cost is remarkably reduced, a feasible technical path is provided for industrial biological manufacturing of PLP, and the application of polyphosphate in cyclic regeneration of cofactors is expanded.

The application provides an arginine mutant and application thereof in 1,4-butanediamine biosynthesis, and belongs to the field of bioengineering. Escherichia coli The mutant is obtained by site-directed mutagenesis of key site amino acid residues based on wild-type EcAdiA from strain The application improves the enzyme activity stability of arginine decarboxylase in neutral environment, improves the conversion rate and conversion speed of 1,4-butanediamine in neutral environment, and lays a foundation for high-efficiency biosynthesis of 1,4-butanediamine.

The invention discloses an application of polygonatum cyrtonema cyclase PcAS1 in promoting synthesis of hematoxylin A. The cyclase PcAS1 is derived from polygonatum cyrtonema, a high isoflavone compound hematoxylin A is synthesized in a plant body, and according to the application, a recombinant plant expression vector for expressing a PcAS1 gene is constructed and transformed into the plant to realize synthesis of the hematoxylin A. Based on multiple omics data such as whole genome, re-sequencing and transcriptome of polygonatum cyrtonema, key cyclase PcAS1 closely related to homoisoflavone biosynthesis is systematically mined. Through a tobacco transient expression system and a polygonatum cyrtonema hairy root transformation system, it is proved for the first time that cyclase PcAS1 can significantly promote synthesis of high isoflavone hematoxylon A, and biosynthesis of hematoxylon A is achieved in polygonatum cyrtonema for the first time.

The application relates to a biosynthesis method of gold nanoparticles, which comprises taking gold ions as gold nanoparticle precursors, taking microorganisms as biocatalysts to carry out a reduction reaction of the gold ions to obtain the gold nanoparticles; the reaction condition is mild, the method is environment-friendly, simple and easy to operate, and the synthesized gold nanoparticles have high biocompatibility and catalytic activity. The gold nanoparticles prepared by the method can be applied to the fermentation hydrogen production of hydrogen production bacteria.

The present disclosure relates to a novel technique capable of highly efficiently producing a high-concentration preparation of a target substance in a production process for the target substance utilizing a microorganism under anaerobic conditions or microaerobic conditions, wherein a necessary substrate is efficiently supplied to a microbial metabolic pathway involved in the biosynthesis of the target substance and the dilution of the target substance in a reaction solution is suppressed. Specifically, the present disclosure relates to a method for producing a target substance, the method including (q) culturing or reacting cells of a microorganism having a urease activity or a treatment product of the cells in a culture medium or reaction medium (X) under anaerobic conditions or microaerobic conditions to produce the target substance, wherein, during the step (q), a urea-containing solution (Y) is added to the culture medium or reaction medium (X).

The invention belongs to the technical field of biosynthesis, and particularly relates to a method for preparing chiral alcohol through asymmetric reduction of large steric hindrance rigid ketone based on aldehyde ketoreductase and a mutant thereof. Comprising the following steps: adding a recombinant Escherichia coli wet cell for expressing aldehyde ketoreductase, a recombinant Escherichia coli wet cell for expressing formate dehydrogenase, a buffer solution, a substrate, a cosolvent, ammonium formate and coenzyme into a reaction container, reacting at a certain temperature, and after the reaction is completed, extracting, separating and carrying out rotary evaporation to obtain a product alcohol. The biological catalysis method is mild in reaction condition, environmentally friendly and high in regioselectivity and stereoselectivity, chiral resolution and heavy metal residues in products are avoided, and the defects of a chemical method are exactly overcome.

The present application relates to a kind of cytochrome P450 oxidase CYP716C52 protein, and the CYP716C52 protein coding gene, the protein can catalyze hydroxylation of maytenic acid C2 position to generate triptolide acid C, in turn participate in triptolide biosynthesis.