1496 results about "Biosynthetic product" patented technology

The invention provides a genetically engineered bacterium for synthesizing fucosyllactose as well as a preparation method and application of the genetically engineered bacterium. The genetically engineered bacterium is prepared by knocking out a glucose-1-phosphate undecanoate transferase gene wcaJ and a beta-galactosidase gene lacZ on the basis of an original strain, the invention relates to an overexpression gene of a phosphomannose gene manB, a phosphoguanine transferase gene manC, a GDP-mannose-4, 6-dehydratase gene gmd, a GDP-fucose synthetase gene WcaG and a beta-galactoside permease gene lacY, and further, the overexpression gene of an alpha-1, 2-fucosyltransferase or an alpha-1, 3-fucosyltransferase is also used for the overexpression of a phosphomannose gene manB, a phosphoguanine transferase gene manC, a GDP-mannose-4, 6-dehydratase gene gmd, a GDP-fucosyltransferase gene. According to the invention, the modified Escherichia coli probiotic Nissle1917 is preferably used as a host for biosynthesis of fucosyllactose, no endotoxin hidden danger exists, and a foundation is laid for industrial production of fucosyllactose.

The invention provides a preparation method and application of salidroside. The preparation method comprises the following steps: culturing an engineering strain for synthesizing the salidroside by using a culture medium containing a mixed carbon source to obtain the salidroside, the mixed carbon source comprises glucose and glycerol. The problem that a salidroside preparation method in the prior art is not suitable for industrial large-scale production can be solved, and the method is suitable for the technical field of biosynthesis.

The invention relates to the technical field of biosynthesis, and particularly discloses a pyridone compound as well as a biosynthesis method and application thereof. On the basis of related technologies of synthetic biology, non-ribosome peptide synthetase is modified, non-ribosome peptide synthetase mutants are heterologously expressed in various hosts, a pyridone compound is found and obtained for the first time, the chemical molecular formula of the pyridone compound is determined to be C5H4N2O3, and the pyridone compound is named as 5-aminopyridine-2, 3, 6-triketone or 5-hydroxy-3-iminopyridine-2, 3, 6-triketone or 5-hydroxy-3-iminopyridine-2, 3, 6-triketone or 5-hydroxy-3-iminopyridine-2, 3, 6-triketone. The novel pyridone compound can be used as a raw material or an intermediate to be applied to development and preparation of novel antibiotics, drugs and dyes.

The invention discloses a tryptophanase mutant and application thereof in production of indole, and relates to the technical field of microbial engineering. Through random mutation and site screening, amino acid replacement is carried out at the 389th site of SEQ ID NO: 1 to obtain a new tryptophan enzyme, and compared with the existing biosynthesis method, the recombinant bacteria constructed by using the gene segment of the modified tryptophan enzyme mutant can efficiently produce indole; meanwhile, the indole production method provided by the invention is low in cost, raw materials are easy to obtain, and the production cost is greatly reduced; the production method provided by the invention is short in fermentation period, simple in process, high in efficiency and not limited by regions. Therefore, the tryptophan mutant and the indole production method provided by the invention have good industrial practicability.

The invention discloses a deoxyartemisinin B synthase and an application of an isoenzyme A0A2U1PPI9 of the deoxyartemisinin B synthase in catalysis of dihydroartemisinin acid for biosynthesis of dihydrodeoxyartemisinin B. The invention further discloses a preparation method of the deoxyartemisinin B synthase. The invention discloses a process for biosynthesizing dihydrodeoxidized artemisinin B from dihydroartemisinin for the first time in a biosynthesis pathway of artemisinin, and bioinformatics analysis and in-vitro activity verification of candidate enzymes show that both the deoxidized artemisinin B synthase and the isoenzyme A0A2U1PPI9 have a new function of catalyzing the biosynthesis of dihydrodeoxidized artemisinin B from dihydroartemisinin, so that the application of the deoxidized artemisinin B synthase and the isoenzyme A0A2U1PPI9 to the biosynthesis of dihydrodeoxidized artemisinin B is developed. Lines of artemisinin biosynthetic pathways are enriched, and a theoretical basis is provided for analysis of terminal biosynthetic pathways of artemisinin and heterologous synthesis of artemisinin compounds.

Composites of metal-organic framework particles and bacterial cellulose, methods of making the composites, and methods of using the composites in the hydrolysis of organic compounds are provided. The composites, which are aerogels comprising metal-organic framework particles embedded in a bacterial cellulose nanofiber network, are fabricated using a microbial synthesis strategy in which bacterial cellulose nanofiber is biosynthesized using a cellulose-producing bacteria in a fermentation medium in which metal-organic framework particles are dispersed.

The invention relates to the technical field of biology, in particular to application of N-terminal truncated 8-isopentenyl transferase in production of 8-isopentene kaempferol. The invention provides the N-terminal truncated 8-isopentenyl transferase, and the N-terminal truncated 8-isopentenyl transferase can be used for carrying out isopentenylation on kaempferol to generate 8-isopentene kaempferol. Specifically, the saccharomyces cerevisiae is used as a chassis, under the condition that kaempferol is exogenously added, through overexpression of 8-isopentenyl transferase, biosynthesis of 8-isopentenyl kaempferol in the saccharomyces cerevisiae is realized, and meanwhile, a foundation is laid for subsequent biosynthesis of icaritin. The invention provides a novel reference scheme for biosynthesis of flavonoid compounds.

The invention provides an application of citrus flavonoid glycosyl transferase CitUGT73A78 in biosynthesis of flavonoid glycoside, and belongs to the technical field of gene engineering and enzyme engineering. According to the present invention, the function of the CitUGT73A78 gene is cloned and verified, and the in vitro enzyme activity test proves that the CitUGT73A78 recombinant protein has broad-spectrum catalytic activity, and can efficiently catalyze the glucosylation of a variety of flavonoids so as to generate the corresponding flavonoids 7-O-glucoside and / or flavonoids 4 '-O-glucoside. An in-vivo transient overexpression test also proves that the CitUGT73A78 gene has a promoting effect on the accumulation of the content of the citrus flavonoid 7-O-glucoside. The invention not only provides a preparation method for the production of flavonoid 4 '-O-glucoside, has the advantages of simplicity, convenience, high catalytic efficiency, low cost, greenness and safety, but also can be applied to nutritional quality improvement and germplasm innovation of citrus fruits.

The invention discloses a tryptophan enzyme mutant and application thereof. The tryptophan enzyme mutant is obtained by mutation of at least one site of the 50th amino acid L, the 394th amino acid V and the 395th amino acid E of a tryptophan enzyme parent. The tryptophan enzyme mutant disclosed by the invention has good substrate bias, particularly has better selectivity and catalytic efficiency on 6 '-halogenated tryptophan, and not only simplifies the production process, but also improves the production efficiency when being used for biosynthesis production of the tylosin.

The invention relates to a method for biosynthesizing zero-valent nano-selenium by utilizing pantoea, the method comprises the following steps of: adding selenite into a strain culture medium, culturing pantoea, and separating and purifying from a synthetic product to obtain the zero-valent nano-selenium, the strain name of the pantoea is XZ-1, the classification name is pantoea Pantoeasp., the preservation number is CGMCC No.32330, the collection number is CGMCC No.32330, the collection number is CGMCC No.32330, the collection number is CGMCC No.32330, the collection number is CGMCC No.32330, the collection number is CGMCC No.32330, and the collection number is CGMCC No.32330. The preservation date is October 24, 2024, the strain XZ-1 is applied to synthesize zero-valent nano-selenium, biological nano-selenium is obtained from the synthesized product after multiple times of separation and purification, and the purified biological nano-selenium is mixed with a protective agent and a dispersing agent according to a certain proportion to prepare a nano-selenium fertilizer raw material, so that the problems that the nano-selenium is agglomerated and is easy to oxidize and lose efficacy are solved; the bioavailability of selenium is obviously improved, and the selenium element loss is reduced. The prepared nano-selenium can be used for original ingredients of fertilizers and feeds.

Provided is a method for biosynthesis of a human structural material type IV collagen. Provided herein is a recombinant collagen, comprising sequences as shown in SEQ ID NO: 1 and the like. The recombinant collagen herein has good cell adhesion activity. The method herein relates to using the genetic engineering technology to produce the recombinant collagen, and overcomes defects in the prior art.

This application relates to the field of biosynthesis technology, and specifically discloses a pyridone compound, its biosynthesis method and application. Based on synthetic biology-related technologies, this application modifies non-ribosomal peptide synthetase, heterologously expresses non-ribosomal peptide synthetase mutants in a variety of hosts, and for the first time discovers and obtains a pyridone compound, and determines its chemical formula as C5H4N2O3, named 5-amino-pyridine-2,3,6-trione or 5-hydroxy-3-imino-pyridine-2,6-dione. This novel pyridone compound can be used as a raw material or intermediate in the development and preparation of novel antibiotics, drugs and dyes.

The invention relates to recombinant streptomyces avermitilis, a construction method of the recombinant streptomyces avermitilis and application of the recombinant streptomyces avermitilis in preparation of combined vermectin B. The recombinant streptomyces avermitilis is streptomyces avermitilis HU510 preserved in China Center for Type Culture Collection (CCTCC), the preservation date is October 9, 2024, and the preservation number of the strain is CCTCC NO: M 20242151. The invention relates to the technical field of synthetic biology, in particular to a method for preparing and producing a novel biological insecticide, and particularly relates to a method for preparing recombinant insect-killing streptomyces of combined vermectin B. The streptomyces sp. Has an aveA3 gene which is inactivated or reduced in activity, and has a functional milA3 gene. According to the invention, efficient production of the combined vermectin B can be realized. According to the invention, a biological synthetic method and technology are utilized to recombine and transform a biosynthetic pathway of abamectin, and the novel insecticidal antibiotic is obtained through screening.

The invention discloses a biosynthetic bacterial cellulose-based solid electrolyte as well as a preparation method and application thereof, and belongs to the technical field of solid electrolytes. The solid electrolyte is prepared by taking modified bacterial cellulose prepared by fermenting acetobacter xylinum, rhizobium, pseudomonas, azospirillum, alcaligenes and other bacteria as a substrate, introducing a low-molecular-weight polymer to regulate and control the arrangement of cellulose molecular chains to serve as a conductive ion framework, and then compounding a conductive ion high-molecular-weight polymer and a lithium salt. According to the invention, cellulose molecular chains are modified by modified glucose, and a network structure is regulated and controlled by a low-molecular-weight polymer, so that the problems of small spacing, lack of ion coordination sites and weak ion transmission capability of pure cellulose molecular chains are effectively solved, the ionic conductivity of the prepared solid electrolyte can reach 10 <-4 > S cm <-1 >, the electrochemical window width reaches 5V, and the solid electrolyte has a good application prospect. The material can tolerate high critical current density, is suitable for various positive electrode materials, and is suitable for high-voltage and long-cycle-life energy storage devices.

The invention discloses a methanophilic genetically engineered bacterium for producing trehalose as well as a construction method and application of the methanophilic genetically engineered bacterium, and belongs to the technical field of industrial biology. The methanophilic genetically engineered bacterium capable of producing the trehalose is obtained by taking methanophilic bacteria methanophilic 5GB1, Alkalicicus glycogenes WONF2802 or methanophilic bacteria NG09 as host bacteria, introducing maltooligosyl trehalose synthase (MTS) into the host bacteria, and carrying out over-expression on maltooligosyl trehalose hydrolase (MTH), so as to obtain the methanophilic genetically engineered bacterium capable of producing the trehalose. Under the condition that methane or methanol serves as a carbon source, the yield of the produced trehalose can reach the gram level or above, a new low-carbon biosynthesis way can be provided for commercial production of the trehalose, and the methanophilic genetically engineered bacterium for producing the trehalose has a good industrial application prospect.

The present invention relates to a recombinant Escherichia coli strain for producing 1, 3-butanediol from glucose and a method for producing 1, 3-butanediol by using the same, in which a recombinant Escherichia coli strain is developed in which a gene of a pathway required for biosynthesis of 1, 3-BDO from acetyl-CoA is cloned, and removing genes interfering with the pathway or a pathway competing with the pathway. Furthermore, a strain of Escherichia coli has been developed in which the pathways and genes involved in the conversion of glucose to acetyl-CoA, the rate of regeneration of NADPH used as a cofactor, and the effective utilization of the TCA cycle pathway are modified and optimized, which are necessary to improve the biosynthesis efficiency of 1, 3-BDO. Therefore, the recombinant Escherichia coli strain of the present invention can produce 1, 3-butanediol from glucose at high concentration, high yield, and high rate.

The invention relates to the field of biological engineering and technology, in particular to an enzyme for synthesizing glabridin, a biological synthesis method of glabridin and a bacterial strain. The invention discloses a series of glabridin synthesizing enzymes (phenylalanine ammonialyase, cinnamic acid-4-hydrogenase, tyrosine ammonialyase, p-coumaric acid coenzyme A ligase, chalcone synthase, chalcone reductase, chalcone isomerase, isoflavone synthase, 2-hydroxyisoflavone dehydratase, 4 '-oxymethyltransferase, 4'-hydroxymethyltransferase and 4 '-hydroxymethyltransferase) derived from glycyrrhiza glabra for the first time. ) can be used in the preparation of a compound (e.g., isoflavone 2 '-hydrogenase, isoflavone reductase, Vissisoketoreductase, isoprenyltransferase, pterocarpin reductase, oxidative cyclase, demethylase, cytochrome P450 reductase, and the like). The de novo synthesis of the glabridin in a microbial cell factory is realized for the first time, and the application prospect of the glabridin biological manufacturing industry is greatly promoted.

The invention discloses a phoebe zhennan PzGGPS12 gene and application of the phoebe zhennan PzGGPS12 gene to improvement of yield and drought tolerance of plant terpenoids, and belongs to the field of genetic engineering, a key enzyme gene PzGGPS12 for catalyzing biosynthesis of the terpenoids in phoebe zhennan wood is screened and analyzed, and a full-length CDS nucleic acid sequence of the PzGGPS12 is obtained. Transgenic experiments prove that overexpression of the gene improves the relative content of tobacco monoterpenes and triterpenes. Short-term drought experiments prove that overexpression of the gene can promote biosynthesis of sesquiterpenes, diterpenes and triterpenes so as to enhance drought tolerance of transgenic tobacco. Therefore, the gene can be introduced into a plant as a target gene, the yield of terpenoids in the plant is increased, and the drought tolerance of the plant is enhanced so as to improve the variety of the plant.

The invention discloses a ferredoxin mutant and application of the ferredoxin mutant in synthesis of murine deoxycholic acid, and belongs to the technical field of biological catalytic enzymes. According to the invention, Escherichia coli is used as a chassis strain to express P450 enzyme OleP, a three-component redox partner library is established, and a redox partner PetH / PetF which is most adaptive to OleP is screened. A high-throughput method based on a characteristic light absorption value is developed, and the lithocholic acid and the murine deoxycholic acid can be rapidly and efficiently detected. And the ferredoxin mutant PetFF64D is screened through semi-rational design of the ferredoxin, so that the conversion rate of catalytically preparing the murine deoxycholic acid can be greatly improved. The method provides a new strategy for improving the biosynthesis of hydroxylated steroids.

The invention relates to a bactericidal composition for asparagus stem blight, which belongs to the technical field of asparagus stem blight and has good prevention, protection and treatment effects. According to the action mechanism, normal growth of pathogenic bacteria is interfered by inhibiting biosynthesis of ergosterol, a good inhibiting effect on pathogenic bacteria of various plants is achieved, and meanwhile, difenoconazole also has a strong inhibiting effect on spore formation of the pathogenic bacteria of the plants; the chitosan oligosaccharide, the carbendazim, the Chinese herbal medicine active extract, the methanol extract, the plant extract, the difenoconazole, the lentinan and the amino-oligosaccharin lentinan mixed preparation have a good prevention and treatment effect on the asparagus stem blight and can block combination of pathogens and sugar molecules on the surfaces of normal cells through an adhesion effect, so that the infection path of the asparagus stem blight is blocked. The lentinan has potential value in the aspect of preventing and treating the asparagus stem blight, and the lentinan enhances the immune function and blocks the pathogen infection pathway.

The invention discloses a production method for biosynthesis of rebaudioside M. Reb A / Reb D has poor solubility at a low temperature, and the reaction efficiency can be obviously accelerated when the Reb A / Reb D is catalytically produced in a high-temperature environment, so that the whole reaction system is carried out in the high-temperature environment, and the production efficiency can be improved. Under the reaction temperature environment of 70 DEG C, the wild type first glycosyltransferase or / and the wild type second glycosyltransferase are / is basically inactivated, and the catalytic enzyme combination cannot meet the requirement of industrial biosynthesis of rebaudioside M. The wild-type catalytic enzyme is mutated, so that the wild-type catalytic enzyme can keep high catalytic activity at 70 DEG C. Therefore, the production cost of the rebaudioside M produced by the production method disclosed by the invention is obviously lower than that of the prior art, and the method is suitable for industrial popularization.

The invention discloses an application of oxygen glycosyl transferase coded by a peucedanum praeruptorum PpUGT5 gene in biosynthesis of scopolin, and belongs to the technical field of biology. The nucleotide sequence of the PpUGT5 gene encodes a nucleotide sequence of an amino acid sequence as shown in SEQ ID NO. 2. The scopolamine is obtained by a biosynthesis method for carrying out enzyme catalysis by utilizing the PpUGT5 protein in vitro, and a new method is provided for synthesizing the scopolamine.

A biosynthesis method for preparing royal jelly acid and the use thereof in skin care, which belong to the technical field of genetic engineering and biosynthesis. The royal jelly acid is produced by means of fermentation on the basis of yeast engineering bacteria using a culture medium containing trans-2-decenoic acid. An assessment at both skin cell level and skin model level shows that royal jelly acid can promote the synthesis of stratum corneum lipids and ceramide on aging skin and repair the skin barrier; and also can promote the synthesis of mitochondria ATP and NADPH in skin fibroblasts, reduce the active oxygen content of mitochondria, improve mitochondrial dysfunctions, and inhibit apoptosis. Further experiments show that royal jelly acid can repair DNA damage of fibroblasts caused by ultraviolet irradiation, reduce telomere shortening caused by cell division and replication, and strengthen the protection of chromosomal terminal regions. The prepared royal jelly acid has wide application prospects in the development of functional skin care products for skin repairing, aging resisting, etc.

The invention provides a high-yield GPP / FPP saccharomyces cerevisiae engineering strain as well as a construction method and application thereof, and relates to the technical field of synthetic biology. Through a gene editing technology, heterologous mvaE and mvaS genes are introduced into saccharomyces cerevisiae, endogenous ERG12, ERG8, ERG19 and ERG20 genes are over-expressed, and the metabolic flux of an MVA pathway in saccharomyces cerevisiae cells is enhanced; meanwhile, a positioning signal tag of peroxisome is added to the gene, and an MVA pathway and a terpenoid precursor synthesis pathway are reconstructed in the peroxisome, so that the compartment synthesis of the terpenoid precursor is realized. Besides, a heterologous MthIPKs gene is further introduced, a branch pathway for IPP synthesis is constructed, higher energy efficiency and less enzymatic reactions are achieved, an MVA pathway and metabolic flux for terpenoid synthesis are concentrated, and the biosynthesis efficiency and conversion rate are further improved.

The application discloses a galactosyltransferase mutant and application thereof in preparation of flavonoid compounds, and belongs to the technical field of enzymology. The application provides a UDP-galactosyltransferase mutant, which is obtained by site-directed mutation of one amino acid in the amino acid sequence of wild-type galactosyltransferase VcUFGT into alanine (A). The effects of a series of mutants H82A, V139A, G141A, P186A, N245A, V282A and S307A are verified in the examples, the mutants can perform enzyme catalysis reaction with quercetin and UDP-galactoside as substrates, and generate flavonoid compound hyperoside, and the specific enzyme activity of the mutants is more than 1.5 times that of the wild type, thereby providing an effective application basis for biosynthesis of flavonoid compounds.

The present invention discloses a broad-spectrum antibacterial peptide, and the amino acid sequence of the antibacterial peptide is GECEGNNRPVKKPQPRKKLPRFKKC-NH2. Based on melittin, the present invention designs antibacterial peptides by predicting based on a deep learning model, and obtains antibacterial peptides with broad-spectrum bacteriostasis, thermal stability and low hemolytic property, which have good inhibitory effects on skin pathogenic bacteria such as Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and Propionibacterium acnes. Peptides predicted as AMP amino acid sequences by the deep learning model antibacterial peptide prediction tool amPEPpy are selected for screening, and by using a biosynthesis technique, they are expressed, extracted and purified in Escherichia coli, reducing the workload and screening period of screening, and increasing the probability of screening out broad-spectrum and thermally stable biosynthetic antibacterial peptides.

The invention discloses application of harringtonine in preparation of anti-rotavirus drugs, and belongs to the technical field of biology. According to the application of the harringtonine in preparation of the anti-rotavirus medicine, the harringtonine has a certain direct inhibition effect and an anti-biosynthesis effect on RV, and the anti-RV effect can be achieved by inhibiting expression of a structural protein VP6 gene.

Provided are biosynthetic human structural material type VI collagen and a preparation method therefor. The collagen comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20 and 21, or a variant thereof, and can be used as a biological dressing, a human bionic material, a plastic surgery material or the like.

The invention discloses a staphylococcus aureus lipase mutant and application thereof, and relates to the technical field of diglyceride biosynthesis. The invention provides six kinds of different staphylococcus aureus lipase mutants SALV358E, SALV358Y, SALV358N, SALP33A, SALP33L and SALY35L, and also provides a method for preparing the staphylococcus aureus lipase mutants. Through detection, compared with the ratio of 0.68 of wild type staphylococcus aureus lipase, the ratios of the six staphylococcus aureus lipase mutants are increased to 1.10, 1.18, 1.22, 1.07, 1.09 and 0.96 by calculating according to the ratio (1, 3-DAG / 1, 2-DAG) of the content of 1, 3-DAG to the content of 1, 2-DAG. Therefore, compared with the wild type, the six staphylococcus aureus lipase mutants provided by the invention have stronger product preference and are more beneficial to the synthesis of 1, 3-DAG.

The invention belongs to the field of application of microbial genetic engineering and metabolic engineering, and particularly relates to a method for improving the biosynthesis efficiency of sclareol. Specifically, in a host strain, gene knockout is realized through a CRISPR / Cas9 technology, or / and a DNA fragment is introduced, and a strain metabolic pathway is adjusted to obtain a corresponding engineering strain; carrying out biosynthesis by utilizing the obtained engineering strain to obtain sclareol; the gene knockout is realized by introducing donor DNA (Deoxyribose Nucleic Acid) containing upstream and downstream homologous arms of a coding frame; the DNA fragment sequentially comprises an upstream homologous arm, a promoter, a gene, a terminator and a downstream homologous arm from upstream to downstream, the host strain is selected from any one of saccharomyces cerevisiae. According to the method, the cell life is associated with product synthesis, and meanwhile, metabolic balance is coupled, so that the synthesis efficiency of a target product is improved, and a foundation is laid for industrial fed-batch fermentation.