137results about How to "Reduce biomass" patented technology

This invention provides isolated or recombinant polypeptides that are useful to vaccinate individuals suffering from chronic / recurrent biofilm disease or as a therapeutic for those with an existing infection. The individual's immune system will then naturally generate antibodies which prevent or clear these bacteria from the host by interfering with the construction and or maintenance of a functional protective biofilm. Alternatively, antibodies to the polypeptides can be administered to treat or prevent infection. Bacteria that cannot form functional biofilms are more readily cleared by the remainder of the host's immune system.

The invention relates to a closed-recirculation multistage biological purification system. The system comprises an aquaculture pond. The aquaculture pond is connected with a multistage biological purification canal through a pond discharge canal, the multistage biological purification canal is connected with a purification tank through a water pump water delivery pipeline, the purification tank is connected with a sedimentation tank through a purification canal, and the sedimentation tank is connected with the aquaculture pond through a self-flow canal. By means of the closed-recirculation multistage biological purification system, point purification and face purification of aquaculture water are achieved; after aquaculture pond discharge water passes through the multistage biological purification system, the content of main nutritive salt and the biomass of plankton become low from high, and a significant point purification effect is achieved; meanwhile, in the whole aquaculture period, various indexes of the overall quality of the aquaculture water are kept at a low level, and a significant face purification effect is achieved; a good scientific example is provided for pond aquaculture in brackish water areas of the coast of China.

The invention discloses an alga control method for a freshwater environment based on cultivation of barracudas and mullets, relating to a water treatment method. Aiming at solving the problem that algae can not be stably and thoroughly controlled in a eutrophic fresh water body, the method comprises the following steps: at least introducing one species of estuarine / marine fishes, such as barracudas and mullets which can only be propagated in sea water, and carrying out desalination processing before cultivation to enable the barracudas and the mullets to be adapted to a freshwater living environment, wherein the mesh size in the large water body ensures whether the cultivated fishes can escape; dividing or mixing according to different ages, carrying out culture in enclosure in girds by meshes, and simultaneously cultivating and propagating silver carps, bighead carps, other local fishes and local shellfish; and propagating local common aquatic plants. Thus, the fishes can be fed on the algae in a filter-feeding mode and simultaneously swallow detritus substances, thereby achieving the purpose of thoroughly purifying the water environment. By harvesting the cultivated and propagated aquatic organisms to remove nitrogen / phosphorus nutrients in water and humus in bed material from the water body, the method disclosed by the invention greatly improves the water quality of the cultivation water body.

The invention relates to an anaerobic-sequencing batch biofilm reactor-artificial wetland method for treating piggery waste water, which is characterized by comprising the following steps: (1) a sewage collecting pipe network collects waste water; (2) the anaerobic environment in a hydrolytic acidification tank enables the organic waste to be subject to biodegradation; (3) an SBBR adjusting tank can achieve the effects of adjusting water quality and water quantity; (4) the waste water is enabled to reach the requirements of GB18596-2001, Discharge Standard of Pollutants for Livestock and Poultry Breeding; and (5) the operation effect of an SBBR reactor and the energy consumption quantity are enabled to reach the optimal combination. The invention has the advantages that the occupied area is decreased; the anaerobic reactor can omit the operating unit required for stabilizing sludge, thereby further decreasing the occupied area and the treatment cost; the waste water treatment effect is better; high denitrification efficiency and stable treatment effect are achieved; and finally, the treatment performance of the combined artificial wetland is fully used to carry out the advanced treatment of denitrification and dephosphorization and meanwhile achieve the effect of reducing the energy consumption of the SBBR system.

The invention belongs to the technical field of environmental protection, and particularly relates to a method and a device for stream guidance and enrichment of blue-green algae in a large water area. With the adoption of the method and the device for the stream guidance and the enrichment of the blue-green algae in the large water area, a certain area which is in shore, and in which the guiding wind is in the downwind direction in the large water area is selected as a blue-green algae stream guidance and enrichment area; a blue-green algae stream guidance and enrichment device is established by utilizing an arched soft algae blocking enclosure and a stream guiding door of which the two sides are in shore; large-scale blue-green algae from outside is guided and gathered to the two sides of the shore by utilizing the arched soft algae blocking enclosure; and the blue-green algae is guided into the enrichment area by utilizing timely opening and closing of the stream guidance door, or the enriched blue-green algae is prevented from blowing and escaping with wind; wind waves in the enrichment area can be reduced by the blue-green algae stream guidance enrichment device, so that the blue-green algae in the enrichment area can always float onto the water surface, and is conveniently to be absorbed and cleared continuously; and in addition, a blue-green algae stream guiding enrichment trap and an efficient algae absorbing area are constructed at one side close to the bottom shore in the blue-green algae stream guidance and enrichment area by utilizing vantage ground, so that the blue-green algae in the enrichment area is further enriched for second time to the area, and is conveniently efficiently absorbed and cleared.

The invention discloses technology for treating piggery wastewater by a methane tank, a biofilm reactor and an artificial wetland. The technology comprises the following steps of: establishing a hydrolytic acidification tank, the methane tank, and a sedimentation tank in front of a sequencing batch biofilm reactor (SBBR) in turn; and establishing an aquatic emergent plant pool and an aquatic cash crop pool. The piggery wastewater flows from the hydrolytic acidification tank and flows out of the aquatic cash crop pool. The time parameters of the SBBR operating condition and technological condition are that: the time for water inlet is 1 hour, the time for anaerobic treatment is 3 hours, the time for aerobic treatment is 7 hours, the time for sedimentation is 0.5 hour, and the time for drainage is 0.5 hour. The wastewater subjected to comprehensive treatment reaches the emission standard.

The invention relates to a composite alga retaining enclosure for floating plants, comprising inner enclosures, outer enclosures and a floating plant community within the enclosed space by the inner enclosures and the outer enclosures. The inner enclosures and the outer enclosures are fixed on inner enclosure fixing piles and outer enclosure fixing piles embedded in bottom mud respectively; the inner enclosures and the outer enclosures are arranged in parallel according to the distance of twice the wave length, each of the outer enclosures comprises a flexible large-aperture nylon meshy hanging slice with the aperture of 50mm, each of the inner enclosures comprises a flexible small-aperture engineering non-woven geotextile hanging slice with the aperture of 50 meshes, the top ends are 1.5m above higher than the high water level. The floating plant community is preferably activated broken branches of alternanthera philoxeroides, and the input density is 20Kg per square meter. The composite alga retaining enclosure for floating plants has the functions of floating island wave decaying, floating plant alga trapping and water purification through the inner enclosures, the outer enclosures and the floating plant community therein, and can reduce the alga biomass at a water intake when being used for retaining alga in water head site; and the water quality of the water head site is improved.

Disclosed is a water mass in-situ recovering ecological reactor, which comprises a rotary plant base system, a rotary biological filler assembly, a wind power propulsion system, and a water-driven biological membrane rotating disc and frame. By utilizing wind power, hydraulic power, solar energy and biomass energy, in-situ recovery can be performed to damaged water masses with high purification efficiency and low operation cost.

The present invention relates to a powdery built biological carrier fluidized bed A / O sewage treatment process. It includes anoxybiotic bio-chemical reaction, aerobic bio-chemical reaction, coagulation reaction and precipitation, in which the anoxybiotic bio-chemical reaction, aerobic bio-chemical reaction, coagulation reaction and precipitation are respectively implemented in anoxybiotic tank, aerobic tank, coagulation tank and precipitation tank which are formed into an integrated reaction tank.

The invention pertains to novel proteins corresponding to Chrysosporium glycosyl hydrolases of families 7 and 10, exhibiting a minimum aminoacid identity of 70 and 75%, respectively, with the amino acid sequence of SEQ ID No's 2 and 4, and to a protein corresponding to a Chrysosporium glyceraldehyde phosphate dehydrogenase, exhibiting at least 86% amino acid identity with the partial amino acid sequence of SEQ ID No. 6. The invention further relates to nucleic acid sequences encoding these proteins, and especially to promoter sequences regulating the expression of the corresponding genes. The preferred host for expressing these genes is a fungus, especially a Chrysosporium strain.

The invention discloses cloning and applications of a key regulation gene GmPHR25 in a plant phosphorus signal network. The nucleotide sequence of the gene is as shown in SEQ ID NO.1, and the amino acid sequence of coded protein is as shown in SEQ ID NO.2. The research shows that overexpression of GmPHR25 can increase the concentrations of soluble phosphorus and total phosphorus of soybean isolated hairy roots and compound plants under treatment of high and low phosphorus, the biomass is improved under the condition of low phosphorus, the biomass of the isolated hairy roots and the compound plants is reduced under the condition of high phosphorus, therefore, GmPHR25 can regulate the growth of transgenic soybeans and the dynamic balance of phosphorus in the body of gene soybeans, and plays an important role in adapting to low-phosphorus stress of plants; GmPHR25 can regulate the adaptation of plants to low-phosphorus stress in soil through a transgenic technology, and also can be used for genetic improvement of leguminous crops for adapting to acid soil, thus having a very important market prospect.

The invention relates to a device for catching planktons, comprising nets and a collector case. The collector case is made of screen net and takes the shape of a case with four walls, a closed bottom and an open top; one side of the peripheral wall of the collector case is cut with an opening, the outer side of which is connected with two nets made of screen net and forming certain angle. The top parts of the nets and the collector case are both provided with a supporting object which ensures the net and the collector case to float and be fixed in place. The invention makes use of water flow generator to push planktons in water into the two nets and sends the planktons into the collector case by the joint function of the water flow and the nets. The planktons are further concentrated in the collector case and workers can fish out the planktons which can be used in feeding aquatic fishes after being packed and transported in the collector case with a small fishing net, realizing the induction, pushing, collecting and fishing of planktons in natural waters or large artificial culture pond.

T-DNA tagging with a promoterless beta-glucuronidase (GUS) gene generated transgenic Nicotiana tabacum plant that expressed GUS activity either only in developing seed coats, or constitutively. Cloning and deletion analysis of the GUS fusion revealed that the promoter responsible for seed coat specificity was located in the plant DNA proximal to the GUS gene. Analysis of the region demonstrated that the seed coat-specificity of GUS expression in this transgenic plant resulted from T-DNA insertion next to a cryptic promoter. This promoter is useful in controlling the expression of genes to the developing seed coat in plant seeds. Similarly, cloning and characterization of the cryptic constitutive promoter revealed the occurrence of several cryptic regulatory regions. These regions include promoter, negative regulatory elements, transcriptional enhancers, core promoter regions, and translational enhancers and other regulatory elements.

The invention provides a high-quality preservation method for a cordyceps militaris strain. The method is characterized in that the cordyceps militaris strain is preserved in a form of bacterial suspension or sclertium under the condition that the temperature is 4 to 25 DEG C, and the preservation period can reach 12 months or above. According to the preservation method disclosed by the invention,quality and stability of the cordyceps militaris strain are improved by optimizing preservation conditions of a preservation form, protecting agent types, temperature range, time period and the likeof the cordyceps militaris strain, and technical guarantee is provided for strain preservation, artificial cultivation and the like.

The invention pertains to novel proteins corresponding to Chrysosporium glycosyl hydrolases of families 7 and 10, exhibiting a minimum aminoacid identity of 70 and 75%, respectively, with the amino acid sequence of SEQ ID No's 2 and 4, and to a protein corresponding to a Chrysosporium glyceraldehyde phosphate dehydrogenase, exhibiting at least 86% amino acid identity with the partial amino acid sequence of SEQ ID No. 6. The invention further relates to nucleic acid sequences encoding these proteins, and especially to promoter sequences regulating the expression of the corresponding genes. The preferred host for expressing these genes is a fungus, especially a Chrysosporium strain.

The invention provides a method for substituting for flaveria bidentis by utilizing grain amaranth; in the method, the grain amaranth is adopted to carry out biological substitution to the flaveria bidentis; the method can lead the germination rate of the flaveria bidentis to be reduced by 80.43 percent, the average plant height is reduced by 77.30 percent, the average single plant branches are reduced by 98.96 percent, the average single plant leaves are reduced by 99.14 percent, the average single plant biomass liveweight is reduced by 92.54 percent, the average soluble protein content is reduced by 52.99 percent, and the method has remarkable control function to the foreign invasion of the flaveria bidentis; the grain amaranth is a common plant, the seed is easy to obtain, the planting method is simple, and the operation is easy; the grain amaranth is pasture with good quality, while controlling the population quantity of the flaveria bidentis, the grain amaranth has a certain economical value.

The invention belongs to the technical field of fuel preparation, and particularly relates to straw fuel containing biomass sludge and a preparation method of the straw fuel. The straw fuel containing the biomass sludge is prepared from the biomass sludge containing straw, a mixture of membrane treatment supernate and straw, lignin and cellulose mud. According to the straw fuel, organic waste obtained through biomass resource treatment is fully utilized, and great economic benefits and environmental friendliness benefits are achieved. In addition, straw particles and other plant materials are added into sludge which is not dewatered, and the thermal value of the dewatered sludge can be increased; due to the fact that the straw particles and other plant fibers are mixed in the sludge, the friction force of the pre-dewatered sludge in a spiral squeezing dehydrator is increased, then the operation frequency of the spiral squeezing dehydrator can be improved, and the operation efficiency and output sludge dryness of the machine are effectively improved.

The invention discloses an immobilized biochar-microalgae material. The immobilized biochar-microalgae material is immobilized microspheres obtained by embedding the microalgae and the biochar with gel microspheres. The microalgae and the biochar are embedded simultaneously, so that the immobilized microspheres are higher in mechanical strength and better in stability and mass transfer performance. The pore structure of the biochar can accommodate the microalgae, improve the immobilization strength and provide nutrient components required by the microalgae. The microalgae and the biochar are coupled in a synergistic manner, so that the removal efficiency of immobilized spheres is improved on nitrogen, phosphorus, organic carbon, heavy metals, etc., the immobilized spheres have good reusability, and the service life of the immobilized spheres in the wastewater treatment is prolonged. The invention discloses application of the immobilized biochar-microalgae material in the wastewater treatment and treatments on nitrogen, phosphorus, organic carbon and heavy metals, with the advantages of high efficiency, good reusability, etc. The invention also discloses a preparation method of theimmobilized biochar-microalgae material, so that the immobilized microspheres with high removal rates of nitrogen, phosphorus, organic carbon and heavy metals in wastewater, good reusability and longservice life can be prepared.

The invention discloses a method for controlling a whole process of cyanobacterial bloom development in a large shallow lake. According to the method, the cyanobacterial bloom development is divided into five stages, namely cyanobacterial wintering period, a recovery period, a rapid growth period, an outbreak period and a cyanobacterial decline period, the spatial distribution characteristics of cyanobacteria in different stages of the cyanobacterial bloom development are determined, and different treatment methods are adopted for different stages of the cyanobacteria bloom development. By themethod, a method for systematically preventing and controlling the whole process of the cyanobacteria bloom development is provided for the first time, prevention and control of the cyanobacteria areextended all the year around, the current situation that the effect is very weak because the prevention and control of the cyanobacteria only aims at cyanobacterial bloom in summer is overcome, and the occurrence intensity and the probability of the cyanobacterial bloom can be effectively reduced throughout the whole year and in the whole process.

The invention relates to a device capable of controlling redox reduction potential to promote sludge dry method anaerobic fermentation, the device can control the redox reduction potential to promote sludge dry method anaerobic fermentation, mainly comprises a fermentation tank, a stirrer, a motor, an anode, a cathode, a low-voltage direct current power supply, a wires and a carbon fiber conductive material, and is characterized in that: the stirrer is located horizontally in the axis of the fermentation tank, an axial stirring rod is sleeved and connected with the anode, the cathode is located on the wall of the fermentation tank, the carbon fiber conductive material is paved on the inner wall of the fermentation tank body, the low-voltage direct current power supply is arranged outside the fermentation tank body, and the anode and the cathode are connected through two wires. In order to improve the methane yield of sludge dry anaerobic fermentation, the redox reduction potential of the inner wall of the fermentation tank body can be controlled at -400mV by passing the additional direct current power supply through the carbon fiber conductive material so as to provide optimum growth redox environment for methane producing bacteria to achieve the object of methane producing bacteria enrichment and methane yield improvement. The solid content of the dry method fermented sludge is 10 ~ 20%.

The invention relates to a treatment method of treating cadmium contaminated water, and relates to a treatment method of contaminated water. The treatment method comprises the following steps of: planting one or two of a cadmium hyperaccumulator plant, namely nightshade, and leaf beta vulgarisvar.cicla into water to be treated; activating and absorbing cadmium contained in the contaminated water; continuously extracting the cadmium contained in the contaminated water by continuously and regularly collecting and replacing the plants till the cadmium content of the water achieves a safety standard; and culturing nightshade and leaf beta vulgarisvar.cicla plants: yielding the nightshade and leaf beta vulgarisvar.cicla plants through seed germination and greenhouse culture. One or two of the nightshade and the leaf beta vulgarisvar.cicla is or are planted in the water to be treated, and a planting method comprises the following steps of: planting the plants into a floating bed, wherein roots directly contact with the contaminated water through small holes drilled on the floating bed; and continuously and regularly collecting and replacing the plants, and collecting and replacing the plants for one time every 15-20 days till the cadmium content of a water body achieves the safety standard. According to the invention, the two plants have the advantages of hyperaccumulation capacity on the cadmium, root system upgrowth, vigorous growth, easiness for propagation and higher biomass, so that the cadmium contaminated water body is efficiently restored.

This invention relates to the treatment of arsenic-containing wastewater. In this invention, the procedures are: collecting water hyacinth, and then washing, drying and cutting the roots, crushing roots into powder. Said powder and arsenic-containing water are fed into a vessel, agitating them intermittently. Finally, the powder and water are separated, to fulfil the treatment. The advantages of this invention are: simple operation, low cost, short treatment period, wide field of application, safety and suitable for commercialized treatment of wastewater.

The invention discloses an ecological net cage-based water purification method; a double-layer net cage is used as a carrier; filter-feeder fish is fed in the upper-layer cage; submerged plants are fixed in the lower-layer cage; the net cage is put into a water area for water purification. The method of the invention is based on the net cage and the comprehensive utilization of water environment ecological systems; no additional energy is necessary; only solar energy and biological energy in the water environment are used to maintain the operation of the net cage module; and no secondary pollution problem is generated. The method can relieve the water bloom problem caused by water eutrophication, can effectively decrease nitrogen and phosphorus concentrations of water body, needs little manual intervention, has the advantages of simple operation and low cost, and is suitable for ecological management and improvement of water of lakes, rivers, reservoirs.

The invention discloses a composite biological filter for treating high algae-laden and high ammonia-nitrogen raw water and an application method thereof. An overflow pipe, a water inlet unit, a sampling opening, a carrier filling area, a water outlet unit and an aerating device are arranged on a composite biological filter body unit from top to bottom in sequence, and suspended spherical biological filter materials, zeolite filter materials, granular active carbon filter materials and a supporting layer are arranged in the carrier filling area from top to bottom in a graded mode. The composite biological filter has the advantages that the spherical biological filter materials can effectively enrich a biological film to remove nitrogen elements and other pollutants in polluted raw materials, algae can be synchronously removed through adsorption, mechanical entrapment and other functions of zeolite and activated carbon, the head loss of an aerating filter can be effectively reduced through a special composite filter material structure, and the back flush frequency is lowered. Meanwhile, the aerating mode, hydraulic retention time and other technological parameters are optimized, and fast biofilm culturing decontamination of the spherical biological filter materials and efficient and low-consumption stable running of a system are achieved.

The invention discloses an algae-controlling and water-purifying biocenosis, relates to a composition, types and an action method of an algae-controlling and water-purifying biotechnological product and belongs to the field of blue-algae governance of environmental-friendly water treatment. The algae-controlling and water-purifying biocenosis comprises one or more marine fishes and one or more freshwater aquatic animals and is constructed in an inland freshwater area environment by virtue of an artificial method. The algae-controlling and water-purifying biocenosis comprises algae-controlling and water-purifying key biological specie adopting marine fishes as a characteristic and a key biological chain adopting marine fishes as a characteristic is constructed, the algae-controlling and water-purifying effects are compounded and the problem of blue-algae inundation is solved in an in-situ manner, so that no risk of biological invasion is caused, the diversities of aquatic organisms in a freshwater area can be increased, detritus substances in the sediment can be recycled, the water can be purified, the high-class aquatic protein can be increased and the economic and social values are also increased; the algae-controlling and water-purifying biocenosis has the advantages of high purification treatment efficiency and good, lasting and stable algae-controlling effect; the method has the advantages of low cost, simplicity in operation and no secondary pollution and is suitable for being widely applied in eutrophicated freshwater and brackish water areas.

The invention discloses a method for improving the salt resistance of tall fescue by virtue of an air-dried sludge microbial agent. The microbial agents comprise Pseudomonas stutzeri + Trichoderma reesei + 100-fold dilution of Streptomyces flavus or Pseudomonas stutzeri + Trichoderma reesei + 200-fold dilution of Streptomyces flavus. The experimental results show that the plant height, biomass and chlorophyll content of tall fescue can be significantly improved, the contents of malonaldehyde and proline are lowered and the protective enzyme activity of tall fescue is enhanced by applying sludge complex microbial agents. On the whole, the microbial agents exhibit better effects in low fertility and the salinized soil, which fully shows the potential applications of microbial agents in the saline-alkali soil improvement.

The present invention relates to an optical path variable plate-type microalgae culture reactor, which comprises a ventilation device and an optical path adjustable photo biological reaction system during a culture process, wherein the ventilation device comprises a flow meter, a ventilation pipe and an aeration device, and the introduced gas is air containing a certain concentration of carbon dioxide (0.04-10% by mass). According to the present invention, the culture device is the simple flat plate-type photobioreactor with the lined transparent plastic bag, and during the culture process, along with the increase of the microalgae cell concentration, the distance between the baffles of the plate-type reactor is changed so as to continuously change the optical path, such that the requirement of the algae cell growth on the optical energy can be met, and the reactor is especially for the culture using sunlight as the optical source.

The invention relates to a method for realizing autotrophic deep denitrification of nitrogen-containing wastewater by utilizing an anaerobic membrane bioreactor (MBR). The method comprises the following steps: S1, under an anoxic condition with a temperature of 20-35 DEG C and a pH value of 7.6-7.8, taking an anaerobic MBR as a reactor to repeat the following operations: introducing artificially synthesized wastewater with sulfides as a sulfur source into a sludge-water mixed solution of sulfur autotrophic denitrification activated sludge, carrying out stirring and domesticating treatment, anddischarging water, wherein the period is 18-24 hours, and domestication is completed when the removal rate of NO3<-> in effluent water is greater than or equal to 94%; and S2, introducing mixed wastewater of artificially synthesized wastewater and nitrogen-containing wastewater, carrying out stirring treatment under anoxic condition with a temperature of 20-35 DEG C and a pH value of 7.6-7.8, andthen carrying out discharging. Through the method provide by the invention, sewage treatment efficiency is high, the total nitrogen (TN) content in the effluent is less than or equal to 15 mg / L, andthe emission standard that the TN content is less than or equal to 40 mg / L in the (Standard for Pollution Control on the Landfill Site of Municipal Solid Waste) is met.

The invention discloses a strain of bacillus amyloliquefaciens with an inhibiting effect on fusarium oxysporum and application of the bacillus amyloliquefaciens. The fusarium oxysporum (FON) is pathogenic bacteria of watermelon fusarium wilt, and biological control is a crucial method for controlling the watermelon fusarium wilt. A bacterial strain for inhibiting the FON is screened from wheat rhizosphere, and the bacterial strain is Bacillus amyloliquefaciens LZN01 through authentication. Experimental results show that the antibacterial rate of the Bacillus amyloliquefaciens LZN01 to the FONis 57.07%, and the spore germination inhibition ratio of the Bacillus amyloliquefaciens LZN01 to the FON is 86.21%. Under the conditions that the temperature is 20-60 DEG C and the pH value is 4-9, the antibacterial effect of a fermented supernatant fluid of the Bacillus amyloliquefaciens LZN01 is stable, and antibacterial substances of the supernatant fluid contain Myriocin, Sphingofungin E, Sphingofungin F, Sphingofungin C and Gabapentin. Therefore, the Bacillus amyloliquefaciens LZN01 has a better inhibiting ability to the FON, the antibacterial effect is stable, and the development and application value is larger in biological control of the watermelon fusarium wilt.