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High-quality preservation method for cordyceps militaris strain

A technology for Cordyceps militaris mycelium and Cordyceps militaris, which is applied in the field of high-quality preservation of Cordyceps militaris strains, can solve the problems that fruit bodies are prone to bending, the ability to form fruit bodies is reduced, and strains are easily contaminated, and the quality of strains can be improved. and stability, shortening the quality evaluation time, and the effect of plump and rich ascus shells

Active Publication Date: 2018-04-24
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The main problems existing in the Cordyceps militaris strain preservation are at present: the preservation cycle is shorter (3-6 months), easy to pollute (liquid or paraffin oil preservation appears easily polluted and strain death phenomenon), strain quality evaluation time is long ( Generally, it needs to be evaluated by the induction of fruiting bodies, which takes up to 3-4 months), low strain activity (as the storage time prolongs, the ability to form fruiting bodies decreases or even cannot form fruiting bodies, and degenerate strains are prone to appear), induction The fruiting body is poor in stability and quality is reduced (with the storage time, the fruiting body tends to be bent and slender, and cannot form ascus shell, etc.)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Cordyceps militaris strain preservation method optimization—mycelium block method

[0041] 1. Materials and methods

[0042] 1.1 Mycelium block preparation

[0043] The Cordyceps militaris mycelium was inoculated onto the modified potato dextrose agar medium, cultured in the dark for 3 days at 23° C., and then cultured in light for 20 days (light intensity 100 lx). Cut into 3mm×3mm hyphae pieces with a sterile scalpel and place them in a sterile 1.5mL centrifuge tube for later use.

[0044] 1.2 Types of protective agents

[0045] Peptone solution: prepared with water, concentration 0.06g / mL, sterilized by high-pressure steam at 121°C for 20 minutes;

[0046] Pasteurized skimmed milk: imported milk from Germany (purchased from Aishi Chenxi brand);

[0047] Autoclaved skimmed milk: the above-mentioned skimmed milk was sterilized by high-pressure steam at 121° C. for 5 minutes.

[0048] 1.3 Preservation conditions

[0049] Put the cut mycelium block into a ...

Embodiment 2

[0056] Example 2 Cordyceps militaris preservation method optimization—bacterial suspension method

[0057] 1. Materials and methods

[0058] 1.1 Bacterial suspension preparation

[0059] The Cordyceps militaris mycelium was inoculated onto the modified potato dextrose agar medium, cultured in the dark for 3 days at 23° C., and then cultured in light for 20 days (light intensity 100 lx). Use a sterile inoculation needle to inoculate the mycelium and spores of Cordyceps militaris into the improved potato glucose liquid medium, place at 23°C, and shake the bacteria at 150rpm for 3 days, until the content of mycelium balls in the bacterial suspension reaches more than 2000 / 100mL. After shaking the bacteria, divide the bacterial suspension into sterile 1.5mL centrifuge tubes for later use.

[0060] 1.2 Types of protective agents

[0061] Peptone solution: prepared with water, concentration 0.06g / mL, sterilized by high-pressure steam at 121°C for 20 minutes;

[0062] Pasteuriz...

Embodiment 3

[0070] Example 3 Cordyceps militaris preservation method optimization—optimization of preservation period

[0071] According to the optimized preservation scheme screened in Examples 1 and 2, the preferred strain preservation method and preservation period are further studied. Cordyceps militaris preservation method optimization - preservation period is as follows:

[0072] 1. Materials and methods

[0073] 1.1 Mycelium block preparation

[0074] The Cordyceps militaris mycelium was inoculated onto the improved PDA medium, cultured in the dark for 3 days at 23° C., and then cultured in light for 20 days (light intensity 100 lx). Cut into 3mm×3mm hyphae pieces with a sterile scalpel and place them in a sterile 1.5mL centrifuge tube for later use.

[0075] 1.2 Preparation of protection solution

[0076] Peptone solution: prepared with water, concentration 0.06%, sterilized by high-pressure steam at 121°C for 20 minutes;

[0077] 1.3 Optimization method of storage period

...

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Abstract

The invention provides a high-quality preservation method for a cordyceps militaris strain. The method is characterized in that the cordyceps militaris strain is preserved in a form of bacterial suspension or sclertium under the condition that the temperature is 4 to 25 DEG C, and the preservation period can reach 12 months or above. According to the preservation method disclosed by the invention,quality and stability of the cordyceps militaris strain are improved by optimizing preservation conditions of a preservation form, protecting agent types, temperature range, time period and the likeof the cordyceps militaris strain, and technical guarantee is provided for strain preservation, artificial cultivation and the like.

Description

technical field [0001] The invention relates to a method for preserving microbial strains, in particular to a high-quality preservation method for the strains of Cordyceps militaris. Background technique [0002] The application of Cordyceps first originated in China, and it is widely used as precious Chinese medicinal materials and edible fungal resources. Cordyceps militaris (Cordyceps militaris), also known as Cordyceps militaris, Cordyceps militaris, etc., belongs to Ascomycota, Hypocreales, Cordyceps family, Cordyceps genus fungus, is a kind of edible and medicinal Cordyceps. The anamorph of Cordyceps militaris is Paecilomyces militaris, and the anamorph differs greatly in terms of colony characteristics, ability to form sexual fruiting bodies, and sporulation structure at the level of monosperm strains. Cordyceps militaris parasitizes the pupae or larvae of insects such as Lepidoptera and develops into fruiting bodies. Cordyceps militaris is mainly distributed in Lia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/04C12R1/645
CPCC12N1/04C12N1/14
Inventor 梁月孙赫男
Owner SHENYANG AGRI UNIV
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