Method for improving salt resistance of tall fescue by virtue of air-dried sludge microbial agent

A technology of air-drying sludge and tall fescue, applied in the direction of microbial-based methods, horticultural methods, chemical instruments and methods, etc., can solve the problems of land barrenness, damage to cultivated land quality, destruction, etc.

Inactive Publication Date: 2014-07-23
TIANJIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to ensure the quality of turf, at least 2 cm of mellow soil must be shoveled off every time the traditional turf production process is completed. After 3-4 stubbles of turf are continuously produced in the same field, the fertile farmland will be destroyed, and the most fertile organic matter will be destroyed. Soil layers are stripped clean, resulting in barren land
Soil turf rolls not only seriously damage the quality of cultivated land, but also cause a series of ecological and environmental protection problems

Method used

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  • Method for improving salt resistance of tall fescue by virtue of air-dried sludge microbial agent
  • Method for improving salt resistance of tall fescue by virtue of air-dried sludge microbial agent
  • Method for improving salt resistance of tall fescue by virtue of air-dried sludge microbial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0122] (1) Cultivation of strains

[0123] Weigh fresh sludge and prepare 10 by dilution separation method -3 、10 -4 、10 -5 、10 -6 sludge suspension. According to the isolated microorganisms, take different concentrations of sludge suspension (fungi take 10 -3 、10 -4 , Actinomycetes take 10 -4 、10 -5 , bacteria take 10 -5 、10 -6 ), shake well and pipette 0.2 mL into the prepared plate. Fungi use Martin's agar plate; actinomycetes use Gao's No. 1 agar plate; bacteria use beef extract peptone plate. Spread evenly with a sterilized applicator, repeat 3 times for each treatment, and incubate at 28°C. According to the shape, size, color and growth speed of the colonies in the plate, pick as many single colonies as possible from each sample and streak for purification. The strains were classified and counted according to their colony culture traits and morphological characteristics. The dominant strains were determined and stored under serial numbers pending further id...

Embodiment 2

[0133] An air-dried sludge bacterial agent is characterized in that it is composed of the following components:

[0134] 100-fold dilution of Pseudomonas stutzeri + Trichoderma reesei + Streptomyces flavum

[0135] Wherein Pseudomonas stutzeri: Trichoderma reesei: the volume ratio of Streptomyces flavinus is 1:1:1;

[0136] The number of colonies of the 3 strains is (2.15±0.03)×10 9 / ml~ (2.07±0.07)×10 9 / ml, the OD600 nm value of Pseudomonas was 0.592;

[0137] Follow the steps below:

[0138] (1) Development materials

[0139] Select tall fescue with plump and uniform grains ( Festuca arundinacea L.) Seeds are the test material. The matrix saline-alkali soil for the test was taken from the seaside of Huanghua City, Hebei Province.

[0140] (2) Preparation of compound microbial agent

[0141]Preparation of compound microbial bacterial agent: Select Pseudomonas stutzeri, Trichoderma reesei and Streptomyces griseus, purify and culture the isolated strains for 2 generat...

Embodiment 3

[0147] An air-dried sludge bacterial agent is characterized in that it is composed of the following components:

[0148] 200-fold dilution of Pseudomonas stutzeri + Trichoderma reesei + Streptomyces flavum;

[0149] Wherein Pseudomonas stutzeri: Trichoderma reesei: the volume ratio of Streptomyces flavinus is 1:1:1;

[0150] The number of colonies of the 3 strains is (2.15±0.03)×10 9 / ml~ (2.07±0.07)×10 9 / ml, the OD600 nm value of Pseudomonas was 0.592;

[0151] Follow the steps below:

[0152] (1) Development materials

[0153] Select tall fescue with plump and uniform grains ( Festuca arundinacea L.) Seeds are the test material. The saline-alkali soil of the test matrix was taken from the seaside of Huanghua City, Hebei Province.

[0154] (2) Preparation of compound microbial agent

[0155] Preparation of compound microbial bacterial agent: Select Pseudomonas stutzeri, Trichoderma reesei and Streptomyces griseus, purify and culture the isolated strains for 2 g...

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Abstract

The invention discloses a method for improving the salt resistance of tall fescue by virtue of an air-dried sludge microbial agent. The microbial agents comprise Pseudomonas stutzeri + Trichoderma reesei + 100-fold dilution of Streptomyces flavus or Pseudomonas stutzeri + Trichoderma reesei + 200-fold dilution of Streptomyces flavus. The experimental results show that the plant height, biomass and chlorophyll content of tall fescue can be significantly improved, the contents of malonaldehyde and proline are lowered and the protective enzyme activity of tall fescue is enhanced by applying sludge complex microbial agents. On the whole, the microbial agents exhibit better effects in low fertility and the salinized soil, which fully shows the potential applications of microbial agents in the saline-alkali soil improvement.

Description

[0001] technical field [0002] The invention belongs to the technical field of environmental protection, and relates to a method for improving the salt resistance of tall fescue with an air-dried sludge bacterial agent. Background technique [0003] The quantity and quality of lawn is an important symbol of urban greening level. At present, there are many ways to build lawns in China. Although the cost of laying turf is high, it has quick results and is easy to operate. It is suitable for all turfgrasses and is generally accepted by the majority of greening workers. The Beijing Landscaping Bureau stated at the first Beijing-Tianjin Region Turf Roll Industry Forum in 2007 that by 2020, the urban green space rate in Beijing will reach 44% to 48%. Tianjin TEDA Ecological Garden Co., Ltd. also introduced here that the Binhai New Area will be built into a livable ecological city. It is planned that the greening rate will reach 40% and the green coverage rate will reach 35% by 2...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C09K17/00A01G1/00A01G7/06C12R1/885C12R1/38C12R1/465
Inventor 多立安赵树兰王志旭
Owner TIANJIN NORMAL UNIVERSITY
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