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Optical path variable plate-type microalgae culture reactor

A culture reactor and microalgae culture technology, applied in the field of variable optical path plate type microalgae cultivation reactors, can solve the problem of not having to change the optical path, and achieve the effects of satisfying cell growth, shortening the optical path, and high biomass

Inactive Publication Date: 2015-06-17
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] At present, the existing methods adjust the I 0 , but in fact the adjustment of the optical path L will produce a similar effect, but for conventional bag reactors that use rigid light-transmitting materials (organic polymers, glass, etc.) or fixed supports, they do not have the ability to change the optical path

Method used

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Embodiment 1

[0024] Isochrysis Zhanjiang was cultured at an initial inoculation density of 10 million cells per milliliter. The medium was a general-purpose F / 2 medium, the carbon dioxide concentration was 1.4%, and the incident light intensity was 95 μM / m·s -2 . By adjusting the ball screw ④, the transmitted light intensity of the system under the same biomass and different optical paths was measured, see Table 1. The results showed that by adjusting the light path, the utilization of light energy in the culture system was changed.

[0025] Table 1. Transmitted light intensity at different optical paths

[0026]

Embodiment 2

[0028] Isochrysis Zhanjiang was cultured at an initial inoculation density of 10 million cells per milliliter. The culture medium was general F / 2 medium with a carbon dioxide concentration of 1.4% and an initial optical path of 5 cm. After growing to 15 million cells per milliliter, the The optical path of the system is adjusted to 7 cm, the concentration of carbon dioxide is adjusted to 2.1%, and water is added to increase the volume by 40%. The expansion to the production scale is completed in the same device.

Embodiment 3

[0030] Isochrysis zhanjiang was cultured at an initial inoculation density of 10 million cells per milliliter. Under the same conditions as other growth conditions, the cells in a 10 cm light path reactor grew to 30 million cells per milliliter, which was affected by the self-shielding effect, that is The stable phase is reached; and using the reactor of the present invention, under the condition of a light path of 5 cm, the final growth reaches 78 million cells per milliliter.

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Abstract

The present invention relates to an optical path variable plate-type microalgae culture reactor, which comprises a ventilation device and an optical path adjustable photo biological reaction system during a culture process, wherein the ventilation device comprises a flow meter, a ventilation pipe and an aeration device, and the introduced gas is air containing a certain concentration of carbon dioxide (0.04-10% by mass). According to the present invention, the culture device is the simple flat plate-type photobioreactor with the lined transparent plastic bag, and during the culture process, along with the increase of the microalgae cell concentration, the distance between the baffles of the plate-type reactor is changed so as to continuously change the optical path, such that the requirement of the algae cell growth on the optical energy can be met, and the reactor is especially for the culture using sunlight as the optical source.

Description

technical field [0001] This patent belongs to the technical field of microalgae cultivation, and specifically relates to a variable optical path plate type microalgae cultivation reactor. Background technique [0002] Microalgae are autotrophic microorganisms, also known as microalgae plants because of the photosynthesis system in their cells. Microalgae have the ability to efficiently absorb solar energy and fix carbon dioxide to produce biomass (that is, photosynthetic ability); at the same time, as a kind of microorganism, microalgae proliferates faster. As single-celled organisms, microalgae are mainly composed of protein, fat, carbohydrates and nucleic acids. At present, microalgae has become an important breeding bait and widely used by farming enterprises. At the same time, microalgae has also become a source of health food such as carotenoids, proteins, and polysaccharides. As the prospect of fossil energy depletion intensifies, the potential application of microal...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12M1/04C12R1/89C12R1/01
Inventor 薛松陆洪斌褚亚东周建男曹旭鹏
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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