Sand-fixing water-keeping sprout-protecting bacteria preparation of low nutrient tolerance

A nutritional and bacterial technology, applied in the direction of bacteria, biochemical equipment and methods, applications, etc., can solve the problems of pure chemical and physical crust instability, no ecological significance, easy to be destroyed, etc.

Inactive Publication Date: 2006-05-10
XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although purely physical or chemical processes can form a mechanical crust on the sandy soil surface, a crust without biological factors is not ecologically significant, and purely chemical and physical crusts are obviously fragile and unstable. Very vulnerable to destruction (Fuller, 1974; Scott, 1982)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Preparation of liquid culture medium for bacteria resistant to low nutrients:

[0015] Potassium dihydrogen phosphate 0.01%, magnesium sulfate 0.01%, sodium chloride 0.02%, calcium sulfate 0.005%, calcium carbonate 0.2%, carbon 1mg / L, the balance is water, make culture medium according to conventional methods, pH7.0 , wherein the carbon source is glucose;

[0016] The isolated and purified two strains known to be resistant to low nutrition and produce extracellular mucopolysaccharides, Bacillus lentus and Beijienchia derxii, were equally inoculated in the prepared liquid medium, Cultivate at 15°C for 48 hours to prepare the culture stock solution of low-nutrition-resistant bacteria, and then dilute the original culture solution to 25%-75% with water; the components of extracellular mucopolysaccharide produced by low-nutrition-resistant bacteria: constituent cells Among the monosaccharides of exopolysaccharides, glucose, mannose and a glucose isomer are the main ones, f...

Embodiment 2

[0020] Preparation of liquid culture medium for bacteria resistant to low nutrients:

[0021] Potassium dihydrogen phosphate 0.01%, magnesium sulfate 0.01%, sodium chloride 0.02%, calcium sulfate 0.005%, calcium carbonate 0.2%, carbon 10mg / L, the balance is water, make culture medium according to conventional methods, pH7.1 , wherein the carbon source is mannitol;

[0022] The isolated and purified 2 strains known to be resistant to low nutrition and produce extracellular mucopolysaccharides, Bacillus lentus and Beijienchia derxii, were equally inoculated into the prepared liquid medium, Cultivate at 30°C for 60 hours to form the culture stock solution of low-nutrition-resistant bacteria, and then dilute the original culture solution to 25%-75% with water; among them, the components of extracellular mucopolysaccharide produced by low-nutrition-resistant bacteria: constituent cells Among the monosaccharides of exopolysaccharides, glucose, mannose and a glucose isomer are the m...

Embodiment 3

[0026] Preparation of liquid culture medium for bacteria resistant to low nutrients:

[0027] Potassium dihydrogen phosphate 0.01%, magnesium sulfate 0.01%, sodium chloride 0.02%, calcium sulfate 0.005%, calcium carbonate 0.2%, carbon 15mg / L, the balance is water, make culture medium according to conventional methods, pH7.2 , wherein the carbon source is glucose;

[0028] The isolated and purified 2 strains known to be resistant to low nutrition and produce extracellular mucopolysaccharides, Bacillus lentus and Beijienchia derxii, were equally inoculated into the prepared liquid medium, Cultivate at 50°C for 72 hours to prepare the culture stock solution of low-nutrition-resistant bacteria, and then dilute the original culture solution to 25%-75% with water; the components of extracellular mucopolysaccharide produced by low-nutrition-resistant bacteria: constituent cells Among the monosaccharides of exopolysaccharides, glucose, mannose and a glucose isomer are the main ones, ...

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Abstract

The invention relates to a bacteriostatic on under-nourished condition for dune-fixing, water retention, sprout retention, which is about preparing bacterial fluid medium under-nourish resistant firstly, secondly seperating and purifying 2 strain bacteria with mucopolysaccharide outside the productive cells under-nourish resistant from the Guerbantonggute desert in Sinkiang, the Flame Mountain arid desert area of the Tulufan basin in the Kelamayi desert, seeding to the fluid medium prepared equally to get culture stock solution of bacteria under-nourish resistant, diluting said culture stock solution to dilution of 25úÑ-75úÑ with water and spraying directly to the microbial skinning formed on the surface of the mobile sand to fix dune, or seeding perennial plants, for example, Wula'er licorice, Chinese ephedra following the conventional planting method to the soil in desert area, spraying said dilution of 25-75úÑ under-nourish resistant to the surface of the soil to hold water and protect sprouts.

Description

technical field [0001] The invention relates to a low-nutrition-resistant bacterial preparation for sand fixation, water retention and seedling preservation. Background technique [0002] Deserts are threatening the survival and development of human beings in some countries and regions. The desert process has become one of the environments that people are most concerned about today. The desert area in my country accounts for about 8% of the land area; dust has become a new global environmental killer. In my country, sand and dust are not limited to the northwest region, and inland cities such as Nanjing, Shandong, and Tianjin have also become new places for dust and dust to patronize. Therefore, it is urgent to prevent and control desertification. [0003] Soil surface crust is a natural phenomenon that exists widely on the surface of the world, including physical crust and biological crust. Although purely physical or chemical processes can form a mec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C09K17/00C12N1/20
Inventor 潘惠霞程争鸣张元明牟书勇张雪梅齐晓玲王方徐新文
Owner XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
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