Two polyploid centella asiatica varieties and cultivation method thereof

A cultivation method and volume-doubling technology, which is applied to tetraploid, triple-volume Centella species and their cultivation fields, can solve problems such as tight supply of Centella asiatica raw materials, large input and large output, and achieve the effect of reducing nutrient consumption

Active Publication Date: 2013-09-04
桂林亦元生现代生物技术有限公司
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  • Application Information

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Problems solved by technology

Even if artificial planting is carried out, the input is greater than the output, ...

Method used

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Examples

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Comparison scheme
Effect test

Embodiment 1

[0026] 1) Cut off 2cm of terminal buds of adult Centella asiatica plants with strong growth potential, remove the outer leaves, rinse with water for 2-4 times, and then use 0.3% potassium permanganate solution under sterile conditions Soak for 20 minutes, wash with sterile water, then treat with 0.2% mercuric chloride for 5 minutes, and wash with sterile water for 6 times. Induced adventitious buds and callus respectively in conventional differentiation medium, and then transferred to conventional proliferation medium for subculture;

[0027] 2) Select the tissue-cultured subcultured seedlings with strong growth potential, cover the terminal buds or callus of the subcultured seedlings softly with sterilized cotton under aseptic conditions, and then use a sterile dropper to absorb 10 μmol.L of filter-sterilized -1 Amisulamide (chemical name: 3,5-dinitro-N4, N4-propylsulfonamide) solution soaked the cotton completely and cultured it in the dark at a low temperature of 5-10°C for...

Embodiment 2

[0034] 1) Cut off 2cm of terminal buds of adult Centella asiatica plants with strong growth potential, remove the outer leaves, rinse with water for 2-4 times, and then use 0.3% potassium permanganate solution under sterile conditions Soak for 20 minutes, wash with sterile water, then treat with 0.2% mercuric chloride for 5 minutes, and wash with sterile water for 6 times. Induced adventitious buds and callus were respectively connected to conventional differentiation medium, and then transferred to conventional proliferation medium for subculture;

[0035] 2) Select the tissue-cultured subcultured seedlings with strong growth potential and culture them in the dark for 5 days, gently peel off the outer leaves with tweezers under sterile conditions, and then transfer to MS solid culture with 12 μmol.L-1 amisulfurin solution Culture in dark medium at 5-10°C for 2-5 days, then transfer to conventional differentiation medium without amisulidine solution for light culture, light fo...

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Abstract

The invention discloses two polyploid centella asiatica varieties and a cultivation method thereof. The cultivation method comprises the following steps of 1, selecting terminal buds of a diploid centella asiatica adult plant, and carrying out breeding of a tissue cultured seedling or callus tissue, 2, carrying out chromosome doubling induction culture and differentiation culture of tissue subcultured seedling or callus tissue by oryzalin, 3, cutting down novel buds obtained by the differentiation culture and carrying out cluster bud culture, 4, carrying out chromosome doubling identification of the cultured complete plants having roots, stems and leaves, screening the tetraploid plant, and planting the tetraploid plant to obtain the tetraploid centella asiatica variety, 5, carrying out artificial emasculation and pollination hybridization of the tetraploid plant and the diploid plant to obtain hybrid seeds (female parent 4N*male parent 2N or male parent 4N* female parent 2N, wherein F1 is 3x), and 6, breeding the hybrid seeds into complete plants, planting the plants, carrying out chromosome number detection and screening the triploid centella asiatica variety.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a tetraploid, triple-volume Centella species and a breeding method thereof. Background technique [0002] Centela asiatica (L.) Urban, a Umbelliferae plant, is a diploid plant and is mainly produced in Guangxi, Guizhou, Yunnan, Guangdong and other places. It is listed in the "Chinese Pharmacopoeia" over the years. The commonly used traditional Chinese medicines recorded, the whole herb is used as medicine. Its taste is bitter, pungent, cool in nature, and has the effects of clearing away heat and dampness, detoxifying and reducing swelling. It is the core raw material for the production of Sanjin Tablets, Yuye Jiedu Syrup and other Chinese patent medicines. It is clinically used in the treatment of damp-heat jaundice, heatstroke diarrhea, stone drench with blood, carbuncle sores, and injuries from falls. It is often eaten as herbal tea and vegetables among the people. In recent ye...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01H1/02
Inventor 苏钰琴蒋臻韬黄忠艺
Owner 桂林亦元生现代生物技术有限公司
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