165 results about "Callisia fragrans" patented technology

The invention discloses a method for tissue-culture breeding and wild-simulated cultivation of dendrobium candidum, which comprises: inoculating dendrobium candidum tender straw slices onto a culturemedium for culture under conditions of a temperature of 22 to 24 DEG C and an illuminance of 1,000 to 1,500 Lx to first induce the growth of calles to form protocorms and then promote the multiplicative growth of the protocorms into 5 to 8 centimeter strong seedlings; and moving the seedlings out of a culture room, hardening the seedlings at a normal temperature for 3 to 5 days and transplanting the seedlings into the ground in a green house. The plane basic layer of a cultivation check field in the green house are provided with spaced breather holes; the upper layer of the cultivation check field is a cultivation medium; the seedlings are planted at a row spacing of 12*8cm and a spacing in the row of 12*8cm; in the green house, the temperature is 0 to 28 DEG C, the humidity is 60 to 70 percent and the shade density is 70 to 80 percent; and disease and pest prevention and control are carried out periodically and the dendrobium candidum can be harvested in 3 to 4 years. The method improves the civil year breeding coefficient of the dendrobium candidum by about 10 times, provides enough seedlings for large-scale cultivation, solves the problem of wild-simulated cultivation and realizes scale cultivation.

The invention provides an artificial propagation method of Spathiphylium Floribundum, which not only can maintian high propagation efficiency but also can keep high consistence and feature stability of plants. It includes five steps such as female parent pretreatment, explants selection, original variety production, original variety domestication, multiplication, rooting and transplantation. Rhizome and lateral bud are used as explants, and after disinfection they are inoculated onto a certain medium for inducing callus and adventitious buds, then a complete plant is formed, after induction and multiplication, the breeding speed is accelerated, the evenness is high, a large amount of obtained ramets characteristics of the clonal progeny of original good single plant or block mass are identical.

The invention discloses a method for building a high-efficiency regeneration system of superior corn self-bred line agriculture line 531, belonging to the field of plant genetic engineering and transgenosis breeding. The invention takes an agriculture line 531 rataria as an explant, induces in a callus induction medium and produces an II-type embryonic callus; the II-type embryonic callus is subjected to embryoid induction under light in an embryoid induction medium to produce a green embryoid; then, the green embryoid is transported to a regeneration medium and is cultured into a regeneration plant under light; root induction is carried out in a rooting medium, and acclimatization is carried out in Hogland nutrient solution to ensure that a new thick root grows on the root of the regeneration plant; the root is transplanted to nutritional soil for rejuvenation culture; and finally, the root is transplanted to a land for growing field crops to normally grow and seed. The regeneration technology is suitable for the superior corn self-bred line agriculture line 531 with high application value, can ensure that the superior quality of agriculture line 531 corn can be inherited in the corn transgenosis breeding process, and has an important meaning for functional genome group research.

The invention belongs to the technical field of pharmaceuticals, and relates to an artificial schisandra chinensis seed preparation and seedling culture method. The method can be used for normally germinating artificial schisandra chinensis seeds in a bacterial culture medium. The artificial schisandra chinensis seeds can be prepared according to the following process: inoculating excellent schisandra chinensis explants into a callus induction medium so as to obtain callus, carrying out embryonic type callus induction on the callus, carrying out somatic embryo induction on the embryonic type callus, synchronously culturing and subculturing somatic embryos obtained through induction, carrying out ion exchanging on cotyledon phase somatic embryos so as to form sodium alginate-CaCl2 hydrogel spheres, and wrapping the obtained hydrogel spheres by utilizing deacetylation chitosan and carbendazol micro powder so as to obtain the artificial seeds wrapped by artificial seed coats; and germinating the artificial seeds so as to obtain the schisandra chinensis. The method can be used for greatly culturing seedlings in a short time and is labor-saving and material-saving.

The present invention provides an efficient method for rapid asexual propagation of notoginseng regenerated plants-by using a bioreactor to cultivate notoginseng somatic embryos in large scale. After proliferation of notoginseng embryogenic callus obtained by inducing adventitious roots, under the condition of MS containing 0.5mg/L 2,4-D, 100% of the embryogenic callus produce spherical somatic embryos with a largest number of embryos. 10.0g (about 1.65x10<4>) spherical somatic embryos are cultured in a 3L-bioreactor which contains 1.5L 1/2MS liquid medium without any plant growth regulators, with an aeration rate of 0 .15 vvm. 4 weeks later, the spherical somatic embryos all develop into cotyledonary somatic embryos, and biological mass growth increases to approximately 8 times of the original mass. The cotyledonary somatic embryos obtained by the liquid or solid medium culture, germinate to be normal somatic embryo seedlings in 1/2MS medium containing 2.0mg/LGA3, then subculture in the 1/2MS medium without any plant growth regulators. Well-grown seedlings are transplanted into nutritious cups filled with artificial soil (peat moss and argillaceous rocks (3:1, v: v)), with relatively high acclimatization survival rate in greenhouse.

The invention relates to a tissue culture and rapid propagation method of epimedium wushanense and propagated epimedium wushanense. The method comprises the following steps of: A) performing stratification on epimedium wushanense seeds at low temperature of 0-10 DEG C after disinfection; B) performing disinfection treatment on the seeds after stratification, and stripping out seed embryos with sterile tweezers for later use; C) inoculating the seed embryos after disinfection into an induction culture medium for culture, and enabling the seed embryos to induce calli and further differentiate adventitious buds or enabling the seed embryos to directly induce buds; D) transferring the buds obtained in the step C) into a propagation culture medium, and generating tissue culture cluster buds; and E) transferring small plants grown from the cluster buds obtained by induction and differentiation in the step D) into a rooting culture medium, culturing to form white fibrous roots, and further forming complete plants. According to the method disclosed by the invention, the propagation coefficient of the epimedium wushanense can be improved, the propagation period can be shortened, and the method further has important values for protecting wild epimedium wushanense resources and promoting sustainable utilization and industrialization of the epimedium wushanense.

The invention discloses a method for inducing hybrid larch plant regeneration through in vitro culture of adventitious buds, relates to a method for inducing pine plant regeneration through in vitro culture of adventitious buds, and aims at solving the problems that in the existing tissue culture method of hybrid larch, organogenesis is difficult, plant regeneration rate is low, and adventitious bud induction rate is low. The method comprises the following steps: 1 inoculating pretreated zygotic embryos of the hybrid larch into a culture medium, and carrying out dark cultivation or light cultivation until callus tissue is generated; 2 inoculating the callus tissue into the culture medium, carrying out light cultivation until an adventitious buds grow; 3 cutting the callus tissue with the adventitious buds into blocks, transferring into the culture medium, carrying out subculture for once after 3 weeks, carrying out adventitious bud elongation, transferring into the culture medium, and carrying out stooling of the adventitious buds; and 4 transferring the stooled adventitious buds into the culture medium, and carrying out light cultivation to realize rooting of the adventitious buds. According to the method disclosed by the invention, the induction rate of the adventitious buds is 87.73%; the stooling rate is 75.96%; the rooting rate is 45%; and the method is applied for inducing hybrid larch plant regeneration through in vitro culture of adventitious buds.

The present invention is the method of raising taxol yield of south taxad. Taxad bud as explant after some squamae are removed is inoculated into the improved MS culture medium with 2,4-D 0.5-2.0 mg/L and/or NAA 0.5-3.0 mg/L and cultured in dark at 23-25 deg.c for 35-40 days; and then, loose, greenish or white callus tissue is selected and transferred into improved B5 culture medium with two or three of 2,4-D 0.5-2.0 mg/L, NAA 0.5-3.0 mg/L and KT 0.2-1.0 mg/L and cultured in dark at 23-25 deg.c for another 35-40 days. The callus tissue thus cultured has taxol content up to 0.013%, 62.5% higher than that in callus tissue cultured with stem and leaf of south taxad as explant.

The invention discloses a method for producing calluses of stems and leaves of a tree peony. In the method, the calluses are induced by using an MS (Murashige and Skoog) culture medium; and a 6-BA (Benzyl Aminopurine) cytokinin, an NAA (Naphthyl Acetic Acid) plant growth regulator, a 2,4-D herbicide and other materials are mixed in the MS culture medium. Specific culture medium schemes and hormone constitutions are provided for explants, such as stem sections and leaves of the tree peony; and the optimal material obtaining period of the calluses is elaborated. Because the inducing rates of the calluses of the tree peony are both more than 80%, a better inducing effect is realized.

The invention discloses two polyploid centella asiatica varieties and a cultivation method thereof. The cultivation method comprises the following steps of 1, selecting terminal buds of a diploid centella asiatica adult plant, and carrying out breeding of a tissue cultured seedling or callus tissue, 2, carrying out chromosome doubling induction culture and differentiation culture of tissue subcultured seedling or callus tissue by oryzalin, 3, cutting down novel buds obtained by the differentiation culture and carrying out cluster bud culture, 4, carrying out chromosome doubling identification of the cultured complete plants having roots, stems and leaves, screening the tetraploid plant, and planting the tetraploid plant to obtain the tetraploid centella asiatica variety, 5, carrying out artificial emasculation and pollination hybridization of the tetraploid plant and the diploid plant to obtain hybrid seeds (female parent 4N*male parent 2N or male parent 4N* female parent 2N, wherein F1 is 3x), and 6, breeding the hybrid seeds into complete plants, planting the plants, carrying out chromosome number detection and screening the triploid centella asiatica variety.

The invention relates to a dwarf lilyturf embryoid inducing and culturing method which comprises the following steps: the basal buds of the dwarf lilyturf are adopted as explants for culturing aseptic seedlings on an aseptic seedling medium; the aseptic seedlings are transplanted on the medium of a primary embryoid to induce embryogenic calluses; the embryogenic calluses are sub-cultured and differentiated to form the primary embryoid; the primary embryoid is transplanted on a secondary embryoid medium to induce a secondary embryoid; and then the secondary embryoid is transplanted to a seedling medium to generate a complete plant. In the invention, regulation is carried out on the two directions of forming a complete plant and the secondary embryoid after the dwarf lilyturf primary embryoid is formed, which not only can obtain the complete plant in a short time, but also can improve the planting percent to 95 percent through the regulation on the inducing and differentiation on the secondary embryoid; moreover, the steps are simple, the culturing cost is low and the propagation coefficient is high.

The invention discloses a monkshood-tuber tissue culture and rapid propagation method, which comprises the main steps of selection, disinfection and sterilization of an explant, inoculation and induction of callus, induction of cluster buds, root induction, hardening-seedling and transplanting and the like. By carrying out tissue culturing on stem tips, blades, roots, stem sections, leafstalks, seeds and the like of monkshood-tuber as the explants, and inducing a plant to regenerate and rapidly propagate monkshood-tuber seedlings, the inductivity, the differentiation rate and the rooting rate are higher, and the survival rate of the seedlings after being transplanted in culture soil is high. The method can effectively overcome the problems of poor disease resistance, low and unstable output, varietal complexity and the like of a traditional monkshood variety in the production of the monkshood-tuber, realizes the variety purification and rejuvenation through stem tip detoxification, tissue culture and other means, and provides excellent monkshood varieties, and lays a favorable foundation for the factory and scale production of large quantities of high-quality seedlings required by monkshood GAP (Good Agricultural Practices) bases and the like.

The invention provides an in-vitro regeneration culture method of tree peonies. The in-vitro regeneration culture method comprises the following steps: after sterilizing tree peony seeds, taking mature embryos and inoculating the mature embryos on a 1/2MS culture medium for culturing; after the mature embryos sprout, taking tender hypocotyls; inoculating the tender hypocotyls into an improved MS culture medium and culturing for 15 to 25 days until notches of the hypocotyls are expanded; inoculating hypocotyl subcultures with the expanded notches into the improved MS culture medium for 14 to 30 days until tree peony adventitious buds are directly differentiated on the expanded notches of the hypocotyls; after the tree peony adventitious buds grow to 3cm to 4cm, cutting off the tree peony adventitious buds from roots and inoculating the tree peony adventitious buds on the improved MS culture medium for rooting, so as to finish in-vitro regeneration of the tree peonies. According to the in-vitro regeneration culture method of the tree peonies, provided by the invention, the adventitious buds can be directly produced by the hypocotyls without calluses under the condition of suddenly reliving PEG6000 (Polyethylene Glycol 6000) osmotic stress and increasing the concentrations of Ca<2+> and microelements Mn<2+>, Zn<2+>, Mo<6+> and Cu<2+>, and a callus process is omitted, so that the problems that the adventitious buds are relatively difficult to differentiate by the calluses and the adventitious bud differentiation rate is commonly relatively low are solved.

The invention discloses a huperzia serrata hairy root system preparation and cultivation method, and belongs to the biological cell engineering technology. The method comprises the following steps: tender stems of huperzia serrata is adopted for dedifferentiation treatment of an explant to acquire huperzia serrata calluses; the callused and agrobacterium rhizogenes (DL 1968) containing Ri plasmid are co-cultivated and transferred to an induced medium for induced cultivation after residual fungus liquid is extracted by aseptic paper, and then hairy roots grow at the huperzia serrata calluses; and the explant with the hairy roots is placed in an expansion medium for expanded cultivation of the hairy roots after bacteriostatic cultivation. According to the invention, the biological cell engineering technology is utilized to build a huperzia serrata hairy root system cultivation system to prepare a huperzia serrata hairy root system, so that standardized production of huperzia serrata is realized, wild resources are replaced, the ever-increasing market demand for huperzia serrata is relieved, and the problem of dependency on huperzia serrata import is solved. Therefore, the huperzia serrata hairy root system preparation and cultivation method has great significance on industrialized and commercialized development of medicinal plant hairy roots.

The invention provides a radix pseudostell adventitious root induction method and a radix pseudostell adventitious root tissue culture method of the medicinal technical field, which comprises that (1) a poisoned radix pseudostell explant is cancelled, and is inoculated on a callus tissue induction culture base after being cut under the bacteria-free condition, and the callus tissue is induced under the bacteria-free light-free condition; (2) the callus tissue that is grown fast with light-yellow color is fetched to be arranged on a 1/2MS solid culture base comprising one or more plant hormones of the seradix, the rhodofix and the kinetin to be induced to form a adventitious root under the bacteria-free light-free condition; (3) liquid culture of the radix pseudostell adventitious root-the obtained adventitious root is arranged in a 1/2MS liquid culture base comprising one or more plant hormones of the seradix, the rhodofix and the kinetin to be cultivated. The method of the invention is simple and the efficiency is high; the adventitious root induction rate of the explant of the stem and the leaves of the radix pseudostell reaches more than 90 percent. The adventitious root grows fast with less inoculation and the growth rate can reach more than 46 times after being cultured in a bottle for 18 days.

The invention relates to the technical field of walnut planting, and discloses a seedling growing method for thin-shell walnuts. The method comprises the following steps that 1, cuttage branches are selected and processed, a cuttage notch is rapidly differentiated to form callus, further differentiation is performed under a seedling growing medium to form transfusion tissue, cambium and growing points, root primordia are formed under the preference temperature, and finally germination is performed to form adventive roots; 2, preparation of the proper seedling growing medium is the key of cutting seedlings, and the relations among the ingredients in the seedling growing medium affect nutrient decomposition and release, wherein the matching ratio of peat to other ingredients is quite subtle, the best matching ratio is mastered, the seedling growth speed can be increased, the seedling growth state can be improved, photosynthesis and organic matter formation are accelerated; 3, the cuttage opportunity and post-cuttage management are quite important, excessive nutrients are not adopted before cuttage survival, and the phenomenon that due to the fact that the environment inadaptability of cutting slip roots, the death rate is high is avoided.

The present invention provides an in vitro resveratrol-rich callus tissue of Vitis thunbergii Sieb. et Zucc. which is a callus tissue developed from a tissue culture system containing one or more plant growth regulators (PGRs) and cultured from a stem or a petiole tissue explant of a wild type of V. thunbergii or a cultivated plantlet of V. thunbergii. The cultivated plantlet is in turn derived from a shoot of the wild type of V. thunbergii cultivated in a plantlet culture system containing no PGRs. The in vitro resveratrol-rich callus tissue of V. thunbergii is characterized by its containing at least about 1,000 to 10,000 mg/kg of dried weight of resveratrol, predominantly in the form of trans-resveratrol and/or resveratrol-O-glucoside, and being ready for harvest or subculture in about 30 days. The present invention further provides a method for cultivating the in vitro resveratrol-rich callus tissue, a method for extracting the resveratrol from the in vitro resveratrol-rich callus tissue, and a method for determining the resveratrol amount in the in vitro resveratrol-rich callus tissue by HPLC.

The present invention belongs to the technical field of plant tissue culture during the agricultural production and is an Aralia eleta leaf culture and a plant of Aralia eleta regeneration induction culture medium. The present invention comprises culture medium nutrient element and culture medium hormone, wherein culture medium inorganic salt comprises NH4NO3, NH4H2PO4, KNO3, CaCl2.2H2O, MgSO4.7H2O, Ca(NO3)2.4H2O, EDTA-Fe, KI, MnSO4.H2O, H3BO3, ZnSO4.7H2O, NaMoO4.2H2O, CuSO4.5H2O and CoCl2.6H2O; the hormone comprises BA, IAA, NAA and ZT. The concentration of the inorganic salt contained by the present invention is quite low, which can save the cost nearly two times; the suitable hormone matching and species can effectively promote callus differentiation and the regeneration and the proliferation of leaf bud. The regeneration plant induction rate of the culture medium laminae of the present invention is more than 75 percent.

The invention provides an induction method of callus of a sterile seedling prepared from the root bud of Amomum villosum Lour. The induction method comprises the following steps: taking the sterile seedling of the root bud of the Amomum villosum Lour. as an explant, sterilizing, inoculating and performing induction culture to obtain the callus, wherein an induction medium for induction culture isMS+1.0 to 2.5 mg/L 6-BA+0.5 to 1.5 mg/L 2,4-D+0.5 to 1.5 mg/L NAA; the illumination time of a culture environment is 10 h, the light intensity is 2000 Lx, and the culture temperature is 25 DEG C. Thestem and the root tip of the sterile seedling of the rhizome of the Amomum villosum Lour. Are used as the explant, and the asexual reproduction can retain the complete medicinal properties of a parent, and variation is not easy to generate, thereby making the medicine property stable and ensuring the effect of the drug treatment, and the explant material is easy to obtain and can be taken at any time for breeding culture.

The invention provides a method for cultivating actinidia arguta anther into haplobiont. The method comprises the following steps: (A) collecting flower buds developed by actinidia arguta microspores, carrying out low-temperature refrigeration treatment for 1-10 days, and peeling anther; and (B) putting the anther in a callus tissue induction medium for carrying out dark culture for 20 days or more, culturing the anther for 40 days or more in a plant differentiation medium, and finally transferring the anther into a rooting medium for culturing and rooting to obtain the haplobiont. Through the method for cultivating actinidia arguta anther into haplobiont of the embodiment, multiple homozygous breeding materials are provided for breeding of actinidia arguta; selection and breeding materials are provided; meanwhile, an ideal receptor material is provided for the basic theoretical research; compared with the manners such as an in-vitro microspore culturing manner and a somatic chromosome eliminating manner, the anther culturing method is simple and convenient to operate; a method of artificially culturing in-vitro anther is an effective method for obtaining haplobiont of actinidia arguta; the method is extremely worthy of extensive popularization and application.

The invention relates to a method for culturing rubia yunnanensis tissue; the method comprises the following steps: the terminal bud or lateral bud of a rubia yunnanensis plant is used as explant, and inoculated to the culture medium of MS or 1/2 MS after washed and disinfected; 0.05 to 0.5 mg/L of BA or 0.05 to 0.5 mg/L of KT plus 0.5 to 3.0 mg/L of NAA or 0.5 to 3.0 mg/L of 2, 4-D is used for carrying out dark culture to form callus; the callus is inoculated in culture medium of MS + 3.0 to 5.0mg/L of BA plus 1.5 to 2.0 mg/L of NAA or 1.0 to 3.0 mg/L of KT plus 0.1 to 1.0 mg/L of IAA, the dark culture is carried out for one week and then illumination is given so as to lead the callus to carry out differentiation to form adventitious bud; the culture medium of 1/2 MS is used, 0.5 to 3.0 mg/L of NAA is used, 1g/L of active carbon is added, and illumination is carried out to form the adventitious root. The invention can improve the inductivity of the callus of the rubia yunnanensis tissue, and improves the regeneration of green plant, thus realizing the fast reproduction of seedling factory of the rubia yunnanensis.

The invention relates to a method for regenerating plants with Taraxacum kok-saghyz Rodin leaves. The method comprises steps as follows: the pretreated Taraxacum kok-saghyz Rodin leaves are sequentially subjected to callus induction, callus subculture, adventitious bud induction and rooting culture. With the adoption of the induction method, the propagation coefficient is high, browned and albino seedlings are avoided, the regenerated plants are robust, root systems are well developed, the transplantation survival rate is high, and a large number of regenerated plants can be obtained within a short time.

The invention provides a rapid cultivation method for hydrangea macrophylla container seedlings. The method includes the steps of: 1) selecting healthy and robustly growing and disease-free hydrangeamacrophylla seedlings, scissoring newly growing branches, removing leaves, retaining parts of petioles, and performing cleaning for standby use; 2) conducting immersion disinfection on the branches with an alcohol solution and a mercuric chloride solution in order on a super clean workbench, and rinsing the branches clean; 3) cutting the branches into small pieces, inoculating the small pieces ofbranches into a medium, and conducting culture in a culture room; 4) after culturing for a period of time, sprouting axillary buds, cutting off the sprout tender branches, and inoculating the branchesinto a proliferation medium for culture; 5) after culture for a period of time, forming a small amount of calluses and adventitious buds at the root, dividing the plants into several clusters, inoculating the plants into a clustered seedling induction medium for culture, further inducing adventitious buds and rooting to form multi-branch rooting seedlings, and putting the seedlings in a greenhouse for acclimatization; 6) after acclimatization for a period of time, cleaning the seedlings, and conducting seedling hardening in a full-illumination spray greenhouse; and 7) at the end of seedling hardening, planting the seedlings in a 1 gallon container for next-step cultivation, thus forming a full-plant container seedling product. The rapid cultivation method for hydrangea macrophylla container seedlings provided by the invention eliminates the pinching process, has short cultivation period, does not affect the flowering of the next year, has high consistency, and can achieve full plants,and is suitable for large-scale and factory anniversary production of hydrangea macrophylla container seedlings.

The invention discloses a culture medium for inducing Chenopodium quinoa willd callus and a culture method thereof. The culture medium is prepared from the following components according to a ratio: an MS culture medium, 1.5 to 2mg/L of 2,4-dichlorphenoxyacetic acid, 1.5 to 2mg/L of 6-benzylaminoadenine, 0 to 300mg/L of yeast extract, 600 to 650mg/L of hydrolyzed casein, 25 to 32g/L of sucrose and3.8 to 4.5g/L of plant gel; the method for inducing the Chenopodium quinoa willd callus by adopting the culture medium comprises the following steps: (1) preparing the culture medium and sterilizingfor later use; (2) adding quinoa seeds into a rooting culture medium with added 30g/L of the sucrose and containing 1/2 time of the MS culture medium, and culturing for 5 to 7 days for later use; (3)carrying out primary callus induction; (4) carrying out subculture. The invention establishes an induction system for rapidly inducing differentiation of the Chenopodium quinoa willd callus.