Culture medium for inducing Chenopodium quinoa willd callus and culture method thereof

A technology of callus and medium, applied in the field of medium for inducing quinoa callus, to achieve the effect of firm texture

Inactive Publication Date: 2018-04-03
CHENGDU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no mature quinoa callus rapid induction system, so the establishment of quinoa callus induction culture system is conducive to accelerating the development of quinoa molecular genetics and laying a solid foundation for later molecular genetic breeding

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A medium for inducing quinoa callus, the pH value of which is 6.0, comprising:

[0023] MS medium, 2,4-dichlorophenoxyacetic acid 2mg / L, 6-benzylaminoadenine 1.5mg / L, yeast extract 300mg / L, hydrolyzed casein 600mg / L, sucrose 30g / L and plant gel 4.4g / L.

[0024] Among them, MS medium includes macroelements, trace elements, iron salts and organic elements;

[0025] Macroelements include the following components: NH 4 NO 3 1650.0mg / L, CaCl 2 332.2mg / L, MgSO 4 180.7mg / L, KNO 3 1900.0mg / L and KH 2 PO 4 170.0mg / L.

[0026] Trace elements include the following components: CoCl 2 ·6H 2 O 0.025mg / L, CuSO 4 ·5H 2 O 0.025mg / L, ZnSO 4 ·7H 2 O 8.6mg / L, H 3 BO 3 6.2mg / L, MnSO 4 ·H 2 O 16.9mg / L, Na 2 MoO 4 2H 2 O 0.25mg / L and KI 0.83mg / L.

[0027] Iron salts consist of the following components: FeSO 4 ·7H 2 O 27.8mg / L and Na 2 - EDTA 37.26mg / L.

[0028] Organic elements include the following components: glycine 2.0mg / L, inositol 100.0mg / L, niacin 0.5mg / ...

Embodiment 2

[0035] A medium for inducing quinoa callus, the pH value of which is 6.0, comprising:

[0036] MS medium, 2,4-dichlorophenoxyacetic acid 1.5mg / L, 6-benzylaminoadenine 2mg / L, yeast extract 200mg / L, hydrolyzed casein 650mg / L, sucrose 32g / L and plant gel 4.5g / L.

[0037] Among them, MS medium includes macroelements, trace elements, iron salts and organic elements;

[0038] Macroelements include the following components: NH 4 NO 3 1650.0mg / L, CaCl 2 332.2mg / L, MgSO 4 180.7mg / L, KNO 3 1900.0mg / L and KH 2 PO 4 170.0mg / L.

[0039] Trace elements include the following components: CoCl 2 ·6H 2 O 0.025mg / L, CuSO 4 ·5H 2 O 0.025mg / L, ZnSO 4 ·7H 2 O 8.6mg / L, H 3 BO 3 6.2mg / L, MnSO 4 ·H 2 O 16.9mg / L, Na 2 MoO 4 2H2 O 0.25mg / L and KI 0.83mg / L.

[0040] Iron salts include the following components: FeSO 4 ·7H 2 O 27.8mg / L and Na 2 - EDTA 37.26mg / L.

[0041] Organic elements include the following components: glycine 2.0mg / L, inositol 100.0mg / L, niacin 0.5mg / L, vi...

Embodiment 3

[0048] A medium for inducing quinoa callus, the pH value of which is 5.8, comprising:

[0049] MS medium, 2,4-dichlorophenoxyacetic acid 1.8mg / L, 6-benzylaminoadenine 1.6mg / L, yeast extract 100mg / L, hydrolyzed casein 600mg / L, sucrose 25g / L and plant gelatin Glue 3.8g / L.

[0050] Among them, MS medium includes macroelements, trace elements, iron salts and organic elements;

[0051] Macroelements include the following components: NH 4 NO 3 1650.0mg / L, CaCl 2 332.2mg / L, MgSO 4 180.7mg / L, KNO 3 1900.0mg / L and KH 2 PO 4 170.0mg / L.

[0052] Trace elements include the following components: CoCl 2 ·6H 2 O 0.025mg / L, CuSO 4 ·5H 2 O 0.025mg / L, ZnSO 4 ·7H 2 O 8.6mg / L, H 3 BO 3 6.2mg / L, MnSO 4 ·H 2 O 16.9mg / L, Na 2 MoO 4 2H 2 O 0.25mg / L and KI 0.83mg / L.

[0053] Iron salts include the following components: FeSO 4 ·7H 2 O 27.8mg / L and Na 2 - EDTA 37.26mg / L.

[0054] Organic elements include the following components: glycine 2.0mg / L, inositol 100.0mg / L, niaci...

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Abstract

The invention discloses a culture medium for inducing Chenopodium quinoa willd callus and a culture method thereof. The culture medium is prepared from the following components according to a ratio: an MS culture medium, 1.5 to 2mg / L of 2,4-dichlorphenoxyacetic acid, 1.5 to 2mg / L of 6-benzylaminoadenine, 0 to 300mg / L of yeast extract, 600 to 650mg / L of hydrolyzed casein, 25 to 32g / L of sucrose and3.8 to 4.5g / L of plant gel; the method for inducing the Chenopodium quinoa willd callus by adopting the culture medium comprises the following steps: (1) preparing the culture medium and sterilizingfor later use; (2) adding quinoa seeds into a rooting culture medium with added 30g / L of the sucrose and containing 1 / 2 time of the MS culture medium, and culturing for 5 to 7 days for later use; (3)carrying out primary callus induction; (4) carrying out subculture. The invention establishes an induction system for rapidly inducing differentiation of the Chenopodium quinoa willd callus.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a medium for inducing quinoa callus and a culture method thereof. Background technique [0002] Quinoa (Chenopodium quinoa willd) is an annual dicotyledonous pseudocereal of the family Amaranthaceae (Chenopodium), which originated in Bolivia and Peru, and has been cultivated in the Andes region for more than 5,000 years. Quinoa is recognized by the Food and Agriculture Organization of the United Nations (FAO) as the only single plant that can meet the basic nutritional needs of the human body as a "total nutritional food". Due to its high nutritional value and economic value, quinoa has attracted widespread attention from scientists all over the world in recent years, and a large number of scientists have devoted themselves to research on molecular genetic breeding and variety improvement of quinoa. However, the immature genetic transformation system of ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 吴琪赵钢白雪赵威周保青
Owner CHENGDU UNIV
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