Method of raising taxol yield of south taxad

A technology of southern yew and paclitaxel, which is applied in the field of increasing the yield of paclitaxel in southern yew plants, can solve the problems of medium browning, aggravating the degree of browning, and not increasing the ratio too much, so as to be beneficial to accumulation, browning, etc. The effect of low variability and low contamination rate

Inactive Publication Date: 2003-01-01
SUN YAT SEN UNIV
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AI Technical Summary

Problems solved by technology

At present, taxus plants increase the content of paclitaxel through tissue culture, usually using 1-2 year old branches, leaves, and young stems as explants to induce and culture callus, but the effect is not particularly ideal. On the one hand, the ratio of the paclitaxel content in the callus to that of the raw material is not too large, for example, the growth of the callus induced by the explants of Taxus chinensis stems and leaves, and the paclitaxel content in the callus 0.

Method used

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Embodiment Construction

[0008] The present invention will be further described below through embodiment.

[0009] Table 1 shows the culture medium used in Examples 1 to 4 of the present invention and the induction rate of callus and the yield of paclitaxel. 2,4-D shown in the table 2.0 , NAA 0.5 , KT 0.2 The subscript numbers of etc. represent the concentration (mg / L) of the component in the culture medium respectively.

[0010] The method of each embodiment is as follows:

[0011] 1. Materials

[0012] Buds of southern yew (Taxus chinensis var. mairei).

[0013] 2. Culture method

[0014] Soak the buds of Taxus chinensis in 70% ethanol for about 3 seconds, rinse with sterile water 5 times; remove a small part of the outer scales, insert into the improved MS medium, and cultivate in the dark at 25°C for 40 days; choose loose, light green or white calli in the modified B 5 The medium was subcultured in the dark at 23-25°C for 40 days. The callus was collected, and the paclitaxel content in th...

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Abstract

The present invention is the method of raising taxol yield of south taxad. Taxad bud as explant after some squamae are removed is inoculated into the improved MS culture medium with 2,4-D 0.5-2.0 mg/L and/or NAA 0.5-3.0 mg/L and cultured in dark at 23-25 deg.c for 35-40 days; and then, loose, greenish or white callus tissue is selected and transferred into improved B5 culture medium with two or three of 2,4-D 0.5-2.0 mg/L, NAA 0.5-3.0 mg/L and KT 0.2-1.0 mg/L and cultured in dark at 23-25 deg.c for another 35-40 days. The callus tissue thus cultured has taxol content up to 0.013%, 62.5% higher than that in callus tissue cultured with stem and leaf of south taxad as explant.

Description

technical field [0001] The invention relates to a method for improving the yield of paclitaxel in Taxus chinensis plants by using a specific culture medium. Background technique [0002] Paclitaxel (taxol) is a terpenoid compound extracted from the yew plant, and is one of the most effective anticancer agents recognized today. Since it was first isolated in 1967, it has been shown to be effective in the treatment of leukemia, metastatic breast cancer, ovarian cancer, malignant melanoma, brain cancer, neck cancer and lung cancer, and also in the treatment of testicular cancer, bladder cancer and esophagus cancer. help. It has been hailed by the National Cancer Institute of the United States as the "best anti-cancer agent" developed in the past fifteen years. It is estimated that the global demand for paclitaxel is 200-300Kg per year. However, paclitaxel is mainly prepared from the bark of yew plants, and its content in the original plant is very low, gen...

Claims

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Application Information

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IPC IPC(8): A01H3/00C07D305/14C12N5/04
Inventor 苏应娟王艇吴惠勤葛发欢
Owner SUN YAT SEN UNIV
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