Method of raising taxol yield of south taxad

Abstract

The present invention is the method of raising taxol yield of south taxad. Taxad bud as explant after some squamae are removed is inoculated into the improved MS culture medium with 2,4-D 0.5-2.0 mg / L and / or NAA 0.5-3.0 mg / L and cultured in dark at 23-25 deg.c for 35-40 days; and then, loose, greenish or white callus tissue is selected and transferred into improved B5 culture medium with two or three of 2,4-D 0.5-2.0 mg / L, NAA 0.5-3.0 mg / L and KT 0.2-1.0 mg / L and cultured in dark at 23-25 deg.c for another 35-40 days. The callus tissue thus cultured has taxol content up to 0.013%, 62.5% higher than that in callus tissue cultured with stem and leaf of south taxad as explant.

Description

technical field [0001] The invention relates to a method for improving the yield of paclitaxel in Taxus chinensis plants by using a specific culture medium. Background technique [0002] Paclitaxel (taxol) is a terpenoid compound extracted from the yew plant, and is one of the most effective anticancer agents recognized today. Since it was first isolated in 1967, it has been shown to be effective in the treatment of leukemia, metastatic breast cancer, ovarian cancer, malignant melanoma, brain cancer, neck cancer and lung cancer, and also in the treatment of testicular cancer, bladder cancer and esophagus cancer. help. It has been hailed by the National Cancer Institute of the United States as the "best anti-cancer agent" developed in the past fifteen years. It is estimated that the global demand for paclitaxel is 200-300Kg per year. However, paclitaxel is mainly prepared from the bark of yew plants, and its content in the original plant is very low, gen...

AI Technical Summary

Problems solved by technology

At present, taxus plants increase the content of paclitaxel through tissue culture, usually using 1-2 year old branches, leaves, and young stems as explants to induce and culture callus, but the effect is not particularly ideal. On the one hand, the ratio of the paclitaxel content in the callus to that of the raw material is not too large, for example, the growth of the callus induced by the explants of Taxus chinensis stems and leaves, and the paclitaxel content in the callus 0.008% of dry weight after extraction

On the other hand, when the Taxus plant tissue cells are cultured, the cells often secrete some reddish-brown pigments or phenolic substances into the medium during the dedifferentiation or differentiation process, causing the entire medium to brown. The secondary metabolic pathway overlaps with a part of the plant browning reaction, which further aggravates the degree of browning, thus seriously affecting the stability of paclitaxel biosynthesis