Induction medium for regeneration of aralia elata leaf plants
A technology for inducing culture medium and aralia, applied in the field of plant tissue culture, can solve the problems of different plant species, poor cultivating effect of aralia leaves, different cultivation parts, etc., so as to reduce costs and improve plant regeneration. Induction rate effect
Inactive Publication Date: 2008-06-18
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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Problems solved by technology
At present, MS substrates are generally used for tissue culture in China, but due to different types of cultured plants and different cultured parts, the effect is also different, and the effect on the culture of Aralia japonica leaves is not good.
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[0045] In mid-June, after the buds unfold, take the leaves with a leaf age of 15-20 days, soak them in 70% alcohol for 15 seconds, rinse them twice with sterile water, and then disinfect them with 0.1% mercury chloride solution for 5-8 minutes or 10% NaClO for 8-10 minutes. Rinse 3 to 5 times with sterile water; cut off the tip of the blade during inoculation, keep the petiole and inoculate it on the surface of the culture medium of the present invention; the culture temperature is 24-26°C, the light time is 14-16 hours, and the light intensity is 1800-2000Lx, 20 Subculture once every 30 days.
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The present invention belongs to the technical field of plant tissue culture during the agricultural production and is an Aralia eleta leaf culture and a plant of Aralia eleta regeneration induction culture medium. The present invention comprises culture medium nutrient element and culture medium hormone, wherein culture medium inorganic salt comprises NH4NO3, NH4H2PO4, KNO3, CaCl2.2H2O, MgSO4.7H2O, Ca(NO3)2.4H2O, EDTA-Fe, KI, MnSO4.H2O, H3BO3, ZnSO4.7H2O, NaMoO4.2H2O, CuSO4.5H2O and CoCl2.6H2O; the hormone comprises BA, IAA, NAA and ZT. The concentration of the inorganic salt contained by the present invention is quite low, which can save the cost nearly two times; the suitable hormone matching and species can effectively promote callus differentiation and the regeneration and the proliferation of leaf bud. The regeneration plant induction rate of the culture medium laminae of the present invention is more than 75 percent.
Description
technical field [0001] The invention belongs to the technical field of plant tissue culture in agricultural production. It relates to the core technology of tissue culture and rapid propagation of Aralia longya, and is a medium for inducing plant regeneration of Aralia longya leaves. technical background [0002] Aralia elata (Miq.) Seem., also known as prickly buds and Northeast wild Chinese toon, is a perennial deciduous small tree of Araliaceae, known as the "king of mountain vegetables". Aralia longya is mainly distributed in Northeast my country, and it is also distributed in North Korea, Russia, and Japan. Its germinated shoots are edible, delicious and fragrant, rich in protein, amino acids, minerals, and vitamins. It is the main wild vegetable variety for export in my country. In addition, Longya has a variety of medicinal and health care values. In Chinese medicine, the root bark and trunk bark of Aralia longya are used as medicine. It is flat in nature, bitter i...
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Inventor 赵恒田宋晓宏沈云霞王新华兰志华梁桂荣
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S