Induction medium for regeneration of aralia elata leaf plants
A technology for inducing culture medium and aralia, applied in the field of plant tissue culture, can solve the problems of different plant species, poor cultivating effect of aralia leaves, different cultivation parts, etc., so as to reduce costs and improve plant regeneration. Induction rate effect
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[0045] In mid-June, after the buds unfold, take the leaves with a leaf age of 15-20 days, soak them in 70% alcohol for 15 seconds, rinse them twice with sterile water, and then disinfect them with 0.1% mercury chloride solution for 5-8 minutes or 10% NaClO for 8-10 minutes. Rinse 3 to 5 times with sterile water; cut off the tip of the blade during inoculation, keep the petiole and inoculate it on the surface of the culture medium of the present invention; the culture temperature is 24-26°C, the light time is 14-16 hours, and the light intensity is 1800-2000Lx, 20 Subculture once every 30 days.
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