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Method for inducing calluses of stems and leaves of tree peony

A technology of callus induction and peony, which is applied in the field of callus culture, can solve the problems of low rooting rate and rooting quality, vitrification, difficulty in callus induction, etc., and achieve the effect of high induction rate

Inactive Publication Date: 2011-09-07
INST OF FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
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AI Technical Summary

Problems solved by technology

[0008] There are problems such as difficulty in callus induction, low rooting rate and quality, difficulty in acclimatization and transplanting, browning, vitrification, etc.

Method used

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Experimental program
Comparison scheme
Effect test

specific Embodiment

[0021] Taking different explants as an example, the best induction effect was achieved by using different media and different culture conditions. In the following, different explants are used as objects to elaborate respectively:

[0022] Induction of embryonic callus:

[0023] Cultivation period: every September, after the seeds are formed;

[0024] Pre-cultivation treatment: use a combination of low temperature (4°C) treatment and gibberellin treatment, and use conventional disinfection methods (2% NaClO for 30 minutes, 70% alcohol for 30 seconds, rinse with sterile water for 3-5 times) after disinfection vaccination;

[0025] The best medium: MS+NAA0.1mg / L+6-BA1.0mg / L;

[0026] The callus induction rate reached 100%.

[0027] Stem segment callus induction:

[0028] Material collection period: take young stems, in late March and early April in spring, or young stems formed by germination of seeds;

[0029] Pre-cultivation treatment: use conventional sterilization metho...

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PUM

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Abstract

The invention discloses a method for producing calluses of stems and leaves of a tree peony. In the method, the calluses are induced by using an MS (Murashige and Skoog) culture medium; and a 6-BA (Benzyl Aminopurine) cytokinin, an NAA (Naphthyl Acetic Acid) plant growth regulator, a 2,4-D herbicide and other materials are mixed in the MS culture medium. Specific culture medium schemes and hormone constitutions are provided for explants, such as stem sections and leaves of the tree peony; and the optimal material obtaining period of the calluses is elaborated. Because the inducing rates of the calluses of the tree peony are both more than 80%, a better inducing effect is realized.

Description

[0001] This application is a divisional application of: [0002] Application number: 200910083744.3; Invention name: method for inducing peony callus; application date: 2009.5.11. technical field [0003] The invention relates to a method for culturing callus, in particular to a method for inducing callus from stems and leaves of peony. Background technique [0004] Peony is one of the top ten famous flowers in China, and it is also the national flower of China. It has the reputation of "the king of flowers". Tissue culture of tree peony mainly uses buds to induce plants and explants to induce plants through callus, and the callus induction of tree peony is an important stage for plant regeneration, and a higher callus induction rate is more important for later callus. How to obtain a higher callus induction rate is closely related to the type of medium, the type and concentration of hormones, and the culture conditions. [0005] Callus culture is the most common form of c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 王雁朱向涛
Owner INST OF FORESTRY CHINESE ACAD OF FORESTRY
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