Method for breeding transgenic tetraena mongolica callus and special culture medium thereof

A technology of callus and callus induction, applied in the field of quadrangle wood biology, can solve the problem that no one has studied the transgenic system of quadrangle wood

Inactive Publication Date: 2011-02-16
INST OF BOTANY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0007] At present, the transgenic system of quadrangle wood has not yet been studied

Method used

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  • Method for breeding transgenic tetraena mongolica callus and special culture medium thereof
  • Method for breeding transgenic tetraena mongolica callus and special culture medium thereof
  • Method for breeding transgenic tetraena mongolica callus and special culture medium thereof

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Experimental program
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Effect test

Embodiment 1

[0053] 1. Preparation of explants

[0054] 1. Sterile pre-culture

[0055] Select the Tetraena mongolica seeds that are plump and have embryos (documents that record this material: Wang, G., Q.Q.Lin, et al. (2007). "Tetraena mongolica Maxim can accumulate large amounts of triacylglycerol in phloem cells and xylem parenchyma of stems. " Phytochemistry 68(15): 2112-2117.; the public can obtain from the Institute of Botany, Chinese Academy of Sciences), after soaking in sterile water with 1% Tween for 10min, disinfecting with 70% ethanol for 1min, soaking in 10% sodium hypochlorite for 6-7min, using Rinse with bacteria water 4-5 times. The seeds are inoculated on 1 / 2 MS medium without hormones, and sterile seedlings can be formed in about 10 days.

[0056] 2. Formation of callus and subculture

[0057] Select the young stems of Tetragalus tissue culture seedlings with vigorous growth and seedling age of about 30 days, cut the stems longitudinally with a sterile scalpel in a...

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Abstract

The invention discloses a method for breeding transgenic tetraena mongolica callus and a special culture medium thereof. The method comprises the following steps: (1) transplanting a tetraena mongolica stem into the tetraena mongolica callus induction culture medium, and culturing to obtain the callus; (2) and transforming the callus obtained in step (1) by using Agrobacterium rhizogenes containing target gene to obtain the transgenic tetraena mongolica callus. In the method, tetraena mongolica seeds from Wuhai in Inner Mongolia are selected as the initial experimental material; after sterileculture to obtain sterile seedlings, the young stem section is cut off to induce the callus; and by using the callus as the explant, and extraneous gene is integrated into the genome of tetraena mongolica by using the Agrobacterium rhizogenes mediated method. By optimizing the processes of transforming and screening callus, the invention establishes a tetraena mongolica callus transformation system which has the advantages of simple operation, high efficiency and high speed.

Description

technical field [0001] The invention relates to a method for cultivating transgenic quadrangle tree callus and a special culture medium thereof, belonging to the field of quadrangle wood biotechnology. Background technique [0002] Tetragalus is a small deciduous shrub of the genus Tetragalus in the family Tribulus, and it is a unique single-species plant in my country. It is a strong xerophyte, distributed in the northern part of the Ordos Plateau in the Inner Mongolia Autonomous Region of my country, the south of the Kubuqi Desert, and the rocky low mountains, sandy gravel plateaus, and piedmont alluvial fans in the foothills of the Alabasu Mountains. The ecological conditions are extremely harsh. It can prevent wind and fix sand, maintain water and soil, and play an important role in maintaining the functions of desert ecosystems. It is the most representative ancient and endangered rare plant, but due to low seed setting rate and high embryo abortion rate, its seed forma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04C12N5/10C12N15/82
Inventor 许亦农平文丽吴韩英李敏春
Owner INST OF BOTANY CHINESE ACAD OF SCI
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