Method for breeding transgenic tetraena mongolica callus and special culture medium thereof
A technology of callus and callus induction, applied in the field of quadrangle wood biology, can solve the problem that no one has studied the transgenic system of quadrangle wood
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Embodiment 1
[0053] 1. Preparation of explants
[0054] 1. Sterile pre-culture
[0055] Select the Tetraena mongolica seeds that are plump and have embryos (documents that record this material: Wang, G., Q.Q.Lin, et al. (2007). "Tetraena mongolica Maxim can accumulate large amounts of triacylglycerol in phloem cells and xylem parenchyma of stems. " Phytochemistry 68(15): 2112-2117.; the public can obtain from the Institute of Botany, Chinese Academy of Sciences), after soaking in sterile water with 1% Tween for 10min, disinfecting with 70% ethanol for 1min, soaking in 10% sodium hypochlorite for 6-7min, using Rinse with bacteria water 4-5 times. The seeds are inoculated on 1 / 2 MS medium without hormones, and sterile seedlings can be formed in about 10 days.
[0056] 2. Formation of callus and subculture
[0057] Select the young stems of Tetragalus tissue culture seedlings with vigorous growth and seedling age of about 30 days, cut the stems longitudinally with a sterile scalpel in a...
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