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Rubia yunnanensis tissue culture method

A technology of tissue culture and red ginseng, which is applied in the direction of plant cells, etc., can solve the problems of shortage of medicinal materials, resource depletion, uneven quality, etc., achieve reliable medicinal resource protection, solve the shortage of wild resources, and reduce the effect of cultivation temperature

Inactive Publication Date: 2009-01-28
CHUXIONG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Small red ginseng has not yet been planted on a large scale, and it mainly relies on wild resources. Due to disorderly mining, a large number of resources are on the verge of exhaustion, and the supply of medicinal materials is in short supply. The price is high and the market demand is large.
Wild red ginseng has problems such as different growth years, unstable content of active ingredients, uneven quality, and unstable output.
And the seeds are difficult to germinate (emergence takes 50-60 days), the growth period is too long, and the seed germination rate is low every other year
However, artificial planting mainly adopts the methods of seed propagation, branch cuttings, and division propagation, which have the problems of low reproduction coefficient, large seed consumption, and low survival rate.

Method used

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  • Rubia yunnanensis tissue culture method
  • Rubia yunnanensis tissue culture method
  • Rubia yunnanensis tissue culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The small red ginseng seeds were collected from Chuxiong Xingsheng Pharmaceutical Company. They were dug up and transplanted with soil to the laboratory for pre-cultivation. They were sprayed with carbendazim solution every 2 to 3 days. The culture of the tissue, the method steps are as follows:

[0037] ①Take the terminal buds or lateral buds of the small red ginseng plant as explants, clean and disinfect them, soak in detergent solution for 5 minutes, rinse with tap water for 5 minutes, soak in 70% wine for 10 seconds, and then rinse with 0.2% mercuric chloride Soak for 8 minutes, rinse 8 times with sterile water;

[0038] ②Inoculate the terminal buds or side buds of the cleaned and disinfected small red ginseng into MS or 1 / 2MS medium, use BA 0.05mg / L+NAA 0.5mg / L, culture in dark for 30 days, and form callus;

[0039]③Inoculate the callus formed by terminal buds or lateral buds in MS+BA 3.0mg / L+NAA 1.5mg / L+IAA 0.1mg / L medium, culture in dark for one week, then give ...

Embodiment 2

[0044] Get the material and carry out the cultivation of small red ginseng tissue according to the following method steps:

[0045] ①Take the terminal buds or lateral buds of the small red ginseng plant as explants, clean and disinfect them, soak in detergent solution for 5 minutes, rinse with tap water for 5 minutes, soak in 70% wine for 10 seconds, and then rinse with 0.2% mercuric chloride Soak for 8 minutes, rinse 12 times with sterile water;

[0046] ② Inoculate the terminal buds or side buds of the cleaned and disinfected small red ginseng into MS or 1 / 2MS medium, use BA0.5mg / L+NAA3.0mg / L, culture in dark for 10 days, and form callus;

[0047] ③ Inoculate the callus formed by terminal buds or lateral buds in MS+BA 5.0mg / L+NAA 2.0mg / L+IAA 1.0mg / L medium, culture in dark for one week, then give 2000LX light for 12 hours / day, the culture temperature is 25±2℃, and the callus differentiates to form adventitious buds;

[0048] ④Use 1 / 2MS medium, use NAA2.0mg / L hormone, add ...

Embodiment 3

[0052] Get the material and carry out the cultivation of small red ginseng tissue according to the following method steps:

[0053] ①Take the terminal buds or lateral buds of the small red ginseng plant as explants, clean and disinfect them, soak in detergent solution for 5 minutes, rinse with tap water for 5 minutes, soak in 70% wine for 10 seconds, and then rinse with 0.2% mercuric chloride Soak for 8 minutes, rinse 10 times with sterile water;

[0054] ②Inoculate the terminal buds or side buds of the cleaned and disinfected small red ginseng into MS or 1 / 2MS medium, use BA 0.25mg / L+NAA 1.5mg / L, and culture in dark for 20 days to form callus;

[0055] ③ Inoculate the callus formed by terminal buds or lateral buds in MS+BA 4.0mg / L+NAA 1.8mg / L+IAA 0.5mg / L medium, culture in dark for one week, then give 2000LX light for 13 hours / day, the culture temperature is 25±2℃, and the callus differentiates to form adventitious buds;

[0056] ④ Use 1 / 2MS medium, use NAA 1.0mg / L hormone...

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Abstract

The invention relates to a method for culturing rubia yunnanensis tissue; the method comprises the following steps: the terminal bud or lateral bud of a rubia yunnanensis plant is used as explant, and inoculated to the culture medium of MS or 1 / 2 MS after washed and disinfected; 0.05 to 0.5 mg / L of BA or 0.05 to 0.5 mg / L of KT plus 0.5 to 3.0 mg / L of NAA or 0.5 to 3.0 mg / L of 2, 4-D is used for carrying out dark culture to form callus; the callus is inoculated in culture medium of MS + 3.0 to 5.0mg / L of BA plus 1.5 to 2.0 mg / L of NAA or 1.0 to 3.0 mg / L of KT plus 0.1 to 1.0 mg / L of IAA, the dark culture is carried out for one week and then illumination is given so as to lead the callus to carry out differentiation to form adventitious bud; the culture medium of 1 / 2 MS is used, 0.5 to 3.0 mg / L of NAA is used, 1g / L of active carbon is added, and illumination is carried out to form the adventitious root. The invention can improve the inductivity of the callus of the rubia yunnanensis tissue, and improves the regeneration of green plant, thus realizing the fast reproduction of seedling factory of the rubia yunnanensis.

Description

technical field [0001] The invention belongs to the technical field of plant regeneration through tissue culture technology, and mainly relates to the technical field of tissue culture and seedling differentiation of small red ginseng produced in the Chuxiong area of ​​Yunnan. Background technique [0002] Little red ginseng (Rubia yunnanensis (Franch.) Diels) is the whole herb of Rubiaceae (Rubiaceae) plant Rubia yunnanensis. The wild Chinese medicinal materials commonly used by Li and other ethnic groups are typical ethnic medicines commonly used in clinical practice. The name of the Yi medicine is Naizose. It has the functions of nourishing blood and activating blood, expelling wind and dampness, softening and breaking accumulation, sedative and analgesic, and is mainly used to treat irregular menstruation, anemia, insomnia, bruises, wind-cold-damp arthralgia, muscle and bone pain, hemiplegia, corneal clouding, boils, swelling and pain , psoriasis, tuberculosis, chronic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04
Inventor 邱璐罗春梅
Owner CHUXIONG NORMAL UNIV
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