In-vitro regeneration culture method of tree peonies
An in vitro regeneration and peony technology, which is applied in plant regeneration, horticultural methods, botanical equipment and methods, etc., can solve the problems of general effect and slow in vitro regeneration of peony, and achieve the effect of promoting growth.
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Embodiment 1
[0032] Step 1, after sterilizing the peony seeds, take the mature embryos and inoculate them on 1 / 2MS medium for cultivation;
[0033] Step 2. After the mature embryo germinates, take the young hypocotyl, cut it into 0.4cm long, inoculate it in PEG6000 containing 4% (W / V) and add 1.5 mg / L 6-BA, 0.2mg / L Cultured on the modified MS medium of LIBA and 3% (W / V) sucrose for 15 days until the hypocotyl incision was enlarged;
[0034] Step 3: Inoculate the enlarged hypocotyls at the incision on the modified MS medium supplemented with 1.5 mg / L 6-BA and 0.2 mg / L IBA for 14 days until the hypocotyls are directly differentiated at the incision Adventitious buds of peony;
[0035] Step 4. When the adventitious buds of peony grow to 3cm, cut off the adventitious buds of peony from the root, and inoculate them with 0.2 mg / L NAA, 0.2 mg / L IBA, 50 mg / L proline and 0.1 mg / L nuclear yellow rooting on the improved MS medium of Su, that is, complete the in vitro regeneration of peony.
[0036...
Embodiment 2
[0040] Step 1, after sterilizing the peony seeds, take the mature embryos and inoculate them on 1 / 2MS medium for cultivation;
[0041] Step 2. After the mature embryo germinates, take the young hypocotyl, cut it into 0.6cm long, inoculate it in PEG6000 containing 4% (W / V) and add 2.5 mg / L 6-BA, 0.5 mg / L Cultured on the modified MS medium of LIBA and 3% (W / V) sucrose for 25 days until the hypocotyl incision was enlarged;
[0042] Step 3: Inoculate the enlarged hypocotyls at the incision on the modified MS medium supplemented with 2.5 mg / L 6-BA and 0.5 mg / L IBA for 30 days until the enlarged hypocotyls are directly differentiated into Adventitious buds of peony;
[0043] Step 4. When the adventitious buds of peony grow to 4cm, cut off the adventitious buds from the root and inoculate them in the seedlings supplemented with 0.2 mg / L NAA, 0.2 mg / L IBA, 100 mg / L proline and 3 mg / L riboflavin Rooting on 1 / 2 of the modified MS medium, that is to complete the in vitro regeneration o...
Embodiment 3
[0048] Step 1, after sterilizing the peony seeds, take the mature embryos and inoculate them on 1 / 2MS medium for cultivation;
[0049] Step 2. After the mature embryo germinates, take the young hypocotyl, cut it into 0.5cm long, inoculate it in PEG6000 containing 4% (W / V) and add 2 mg / L 6-BA, 0.35mg / L Cultured on the modified MS medium of LIBA and 3% (W / V) sucrose for 20 days until the hypocotyl incision was enlarged;
[0050] Step 3: Inoculate the enlarged hypocotyls at the incision on the modified MS medium supplemented with 2 mg / L 6-BA and 0.35 mg / L IBA for 22 days until the enlarged hypocotyls are directly differentiated from the incision. Adventitious buds of peony;
[0051] Step 4. When the adventitious buds of peony grow to 3.5cm, cut off the adventitious buds from the root and inoculate them in the seedlings supplemented with 0.2 mg / L NAA, 0.2 mg / L IBA, 75 mg / L proline and 1.4 mg / L nucleus. Rooting on the modified MS medium of flavin, that is to complete the in vitro...
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