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Tissue culture and rapid propagation method of epimedium wushanense and propagated epimedium wushanense

A technology of tissue culture rapid propagation and epimedium, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of slow growth, low germination rate of reproduction, dormancy, etc.

Active Publication Date: 2012-07-18
GUOYAOJITUAN TONGJITANG (GUIZHOU) PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The raw materials of Wushan Epimedium mainly rely on wild resources. With the increasing demand for it at home and abroad, the wild resources of Wushan Epimedium have been severely damaged and cannot meet the needs of domestic and foreign markets.
At present, the propagation methods of Epimedium mainly include: through asexual reproduction of underground rhizomes and seed reproduction (sexual reproduction), Epimedium seeds have obvious dormancy phenomenon, low germination rate of reproduction, and slow growth, most of the production adopts branching reproduce

Method used

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  • Tissue culture and rapid propagation method of epimedium wushanense and propagated epimedium wushanense
  • Tissue culture and rapid propagation method of epimedium wushanense and propagated epimedium wushanense
  • Tissue culture and rapid propagation method of epimedium wushanense and propagated epimedium wushanense

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Embodiment 1

[0058] Embodiment 1: Wushan Epimedium seed treatment and induction test of seed embryo

[0059] 1. Selection and treatment of explants: Remove impurities and unfulfilled particles from the collected Epimedium wushanense seeds, wash them with running water for 30 minutes, soak them in 75% alcohol for 30 seconds, and then soak them in 0.1% HgCl 2 Soak in medium for 6 minutes, rinse with sterile water for 5 times, dry the water with sterile filter paper, and mix with river sand that has been sterilized at 121°C and 0.1Mpa for 2 hours and cooled to room temperature in a ratio of 1:3 to keep the water content at 60%. , after 90 days of low-temperature stratification treatment at 5°C to break seed dormancy.

[0060] 2. Disinfection of explants: Wash the seeds after stratification treatment to remove river sand, soak them in 75% alcohol for 30s, rinse them with sterile water for 5 times, and then use 0.1% HgCl 2 Disinfect for 6 minutes, rinse with sterile water for 5-6 times, dry th...

Embodiment 2

[0071] Embodiment 2: Tissue culture rapid propagation method cultivates Epimedium wushanense

[0072] A. Selection and treatment of explants: Remove impurities and unfulfilled particles from the collected Epimedium wushanense seeds, wash them with running water for 30 minutes, soak them in 75% alcohol for 30 seconds, and then soak them in 0.1% HgCl 2 Soak in medium for 6 minutes, rinse with sterile water for 5 times, dry the water with sterile filter paper and mix with river sand that has been sterilized at 121°C, 0.1Mpa for 2 hours and cooled to room temperature in a ratio of 1:3 to keep the water content at 60%. Seed dormancy was broken after 90 days of low-temperature stratification treatment at 5°C.

[0073] B. Disinfection of explants: Wash the seeds after stratification treatment to remove river sand, soak them in 75% alcohol for 30s, rinse them with sterile water for 5 times, and then use 0.1% HgCl 2 Disinfect for 6 minutes, rinse with sterile water for 5-6 times, dry ...

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Abstract

The invention relates to a tissue culture and rapid propagation method of epimedium wushanense and propagated epimedium wushanense. The method comprises the following steps of: A) performing stratification on epimedium wushanense seeds at low temperature of 0-10 DEG C after disinfection; B) performing disinfection treatment on the seeds after stratification, and stripping out seed embryos with sterile tweezers for later use; C) inoculating the seed embryos after disinfection into an induction culture medium for culture, and enabling the seed embryos to induce calli and further differentiate adventitious buds or enabling the seed embryos to directly induce buds; D) transferring the buds obtained in the step C) into a propagation culture medium, and generating tissue culture cluster buds; and E) transferring small plants grown from the cluster buds obtained by induction and differentiation in the step D) into a rooting culture medium, culturing to form white fibrous roots, and further forming complete plants. According to the method disclosed by the invention, the propagation coefficient of the epimedium wushanense can be improved, the propagation period can be shortened, and the method further has important values for protecting wild epimedium wushanense resources and promoting sustainable utilization and industrialization of the epimedium wushanense.

Description

technical field [0001] The invention belongs to the technical field of cultivation of Chinese medicinal materials, and relates to a method for breeding Epimedium Wushanense, in particular to a method for tissue culture and rapid propagation of Epimedium Wushanense. Background technique [0002] Epimedium wushanense T.S. Ying is a perennial herbaceous medicinal plant of the genus Epimedium in the family Berberidaceae, and it is a species listed in the 2010 edition of the Pharmacopoeia of the People's Republic of China. Its dried leaves are used as medicine, which has the effects of nourishing kidney yang, strengthening muscles and bones, dispelling rheumatism, and has physiological activities such as improving cardiovascular system, regulating endocrine and anti-osteoporosis. The raw materials of Wushan Epimedium mainly rely on wild resources. With the increasing demand for it at home and abroad, the wild resources of Wushan Epimedium have been severely damaged and cannot mee...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 郭巧生周海琴朱国胜周宁刘作易贺勇杨相波
Owner GUOYAOJITUAN TONGJITANG (GUIZHOU) PHARMA CO LTD
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