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Method for breeding polyploid royal paulownia by combination of in vitro culture and colchicine treatment

A technology of colchicine and in vitro culture, applied in the directions of botanical equipment and methods, biochemical equipment and methods, horticultural methods, etc., can solve the problems of low frequency of polyploid trees being difficult to survive, and achieve good viewing and saving. The effect of nutrition

Active Publication Date: 2008-12-17
WUHAN POLYPLOID BIOTECH CO LTD
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

Solve the problem that the survival frequency of polyploid trees is very low, so as to efficiently cultivate polyploid trees

Method used

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  • Method for breeding polyploid royal paulownia by combination of in vitro culture and colchicine treatment
  • Method for breeding polyploid royal paulownia by combination of in vitro culture and colchicine treatment
  • Method for breeding polyploid royal paulownia by combination of in vitro culture and colchicine treatment

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Embodiment 1

[0041] Example 1. Breeding of a new tetraploid Paulownia tomentosa strain "Paulownia tomentosa 108"

[0042] a. Select parent

[0043] The excellent Paulownia tomentosa plant Hongshan 008, which has no arbuscular disease and has flowered, was used as the parent for tissue culture.

[0044] b. Placenta is the best explant

[0045] The stem segments, leaves and placenta in the fruit 30-35 days after flowering are taken from the Paulownia tomentosa plant of Hongshan 008 as explants for tissue culture. The cultured contamination rate (number of polluted explants / total number of inoculated explants×100%), rate of callus induction (number of callus pieces / number of inoculated explants×100%) and rate of differentiation and germination of callus ( The number of differentiated and sprouted callus / callus total number × 100%) was compared and found that the placenta was explant contamination rate was 0, the callus induction rate was 100%, and the callus differentiated and sprouted rate...

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Abstract

The invention provides a method of breeding fourfold paulownia tomentosa in the combination of isolated culture by using placentas as explants and colchicine treatment, comprising the following twelve steps: a. choice of parent; b. using placentas as best explants; c. placenta callus inducement; d. double treatment of placenta callus; e. callus recovery cultivation after treatment; f. callus division cultivation; g. sprout rooting cultivation; h. transplanting of seedling; i. morphological authentication of paulownia tomentosa plant polyploidy; j. chromosome number authentication of paulownia tomentosa plant polyploidy; k. flow cytometry analysis authentication of paulownia tomentosa plant polyploidy; l. authentication of paulownia tomentosa plant polyploidy in flowering period, etc. Compared with adult plants of other multiploid paulownia tomentosa, the fourfold paulownia tomentosa is characterized by big flower, thick leaf, no burliness, and the like, obviously different from twofold paulownia tomentosa, and has perfect ornamental value and growing vantage. The technology not only can be directly applied to breeding new types of paulownia tomentosa but also builds a fine foundation for polyploid breeding of other trees.

Description

technical field [0001] The invention relates to the combined selection and breeding of polyploid paulownia tomentosa by in vitro culture and colchicine treatment, and belongs to the technical field of polyploid tree breeding. Background technique [0002] From the trend of plant evolution, more than 70% of angiosperms are polyploid (Masterson J. Stomatal size in fossil plants: evidence for polyploidy in majority of angiosperms. Science, 1994, 264: 421-424). The giganticity, strong dominance, stress resistance and low fruiting properties of polyploid plants are especially beneficial to the breeding of new tree varieties (Kang Xiangyang. Research progress in polyploid breeding of forest trees. Journal of Beijing Forestry University, 2003, 25 (4 ): 70-74; Li Yun, Feng Daling. Research Progress on Polyploid Breeding of Woody Plants. Bulletin of Botany, 2005, 22(3): 375-382). Paulownia is not only a famous fast-growing timber tree species, but also has the characteristics of blo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/08A01H4/00A01H5/12A01H5/02A01G31/00C12Q1/68C12N5/04
Inventor 蔡得田唐志强陈冬玲宋兆建何玉池
Owner WUHAN POLYPLOID BIOTECH CO LTD
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