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New lentinula edodes strain K48 and breeding method thereof

A new strain and strain technology, applied in botany equipment and methods, horticulture, application, etc., can solve the problems of over-density, unstable transformants, low expression rate, etc., and achieve the effect of improved production traits and obvious mutagenic effect

Inactive Publication Date: 2012-07-04
YUANAN KELISON MUSHROOM
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the main problems currently existing in genetic engineering breeding are: 1. The interference of false positive colonies; 2. The integration rate of exogenous DNA is not high; 3. The expression rate of exogenous genes is not high after integration; 4. The transformant is unstable
Lentinus edodes "135" strain is a low-temperature late-maturing variety used in the 1970s. It has good mushroom shape and quality, but it has been used for a long time. Rotten cylinder
Lentinus edodes "939" strain has a high yield and is easy to manage, but the mushrooms are too much, too dense, and there are many clusters of mushrooms, which seriously affect the quality of lentinus edodes

Method used

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  • New lentinula edodes strain K48 and breeding method thereof
  • New lentinula edodes strain K48 and breeding method thereof
  • New lentinula edodes strain K48 and breeding method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0033] 1. Materials and methods

[0034] 1.1 Materials

[0035] 1.1.1 Parental strains

[0036] Shiitake 135 (introduced by Sanming Fungi), Shiitake 939 (introduced by Sanming Fungi), 303 (crossbreeding by our company), JL-2 (introduced from Japan).

[0037] 1.1.2 Medium

[0038] Test medium: PDA+20% fungus chaff boiled juice.

[0039] Mutation medium: PDA+0.1% colchicine.

[0040] Cultivation medium: miscellaneous sawdust 83%, bran 15%, sugar 1%, gypsum 1%.

[0041] 1.1.3 Main reagents

[0042] Colchicine (C22H25NO 5 ): 98% content, analytically pure, imported by Shanghai Huishi Biochemical Reagent Company.

[0043] Fluorescent dye: Hoechst33258 (trade name), bisbenzimidazole (C 25 h 24 N 5 o 13HCL) chemical name, imported from Switzerland.

[0044] 1.2 Method

[0045] 1.2.1 Single spore isolation

[0046] On December 15, 2004, the fruiting bodies (eight mature) of Lentinus edodes 135 and 303 were collected respectively, placed in a bell-type spore collector, cu...

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Abstract

The invention relates to a new lentinula edodes strain K48 and a breeding method thereof. The new lentinula edodes strain K48 is a new strain obtained by hybridizing a dikaryon with a mutagenized monocaryon of a lentinula edodes strain 303, and is a new strain obtained by carrying out monocaryon induction cultivation through a PDA culture medium containing colchicine and carrying out hybridization; and through a double effect of mutagenesis and hybridization, the production traits, such as yield, quality and adaptability and the like, of the new strain are greatly improved in comparison with an original strain and parent strains, so that the breeding method is a simple, practical and effective edible mushroom breeding new technology.

Description

technical field [0001] The invention relates to a new shiitake mushroom strain K48 and a breeding method thereof, belonging to the field of edible fungus breeding. Background technique [0002] At present, the breeding methods of edible fungi mainly include training of wild species, cross breeding, mutation breeding, protoplast fusion breeding and genetic engineering breeding. The training of wild species is to obtain mycelia from wild fruiting bodies, and obtain excellent strains through cultivation tests. This method needs to separate and obtain a large number of bacterial strains, from which select high-yield and high-quality varieties, and cannot create new species. Hybrid breeding is a widely used and effective breeding technology. It can combine the excellent traits of both parents, show strong hybrid vigor, and breed excellent hybrids that surpass the parents. However, hybrid breeding is a labor-intensive and time-consuming work. The process of determining the paren...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04A01H1/08
Inventor 徐年声
Owner YUANAN KELISON MUSHROOM
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