Method for building high-efficiency regeneration system of superior corn self-bred line agriculture line 531

Abstract

The invention discloses a method for building a high-efficiency regeneration system of superior corn self-bred line agriculture line 531, belonging to the field of plant genetic engineering and transgenosis breeding. The invention takes an agriculture line 531 rataria as an explant, induces in a callus induction medium and produces an II-type embryonic callus; the II-type embryonic callus is subjected to embryoid induction under light in an embryoid induction medium to produce a green embryoid; then, the green embryoid is transported to a regeneration medium and is cultured into a regeneration plant under light; root induction is carried out in a rooting medium, and acclimatization is carried out in Hogland nutrient solution to ensure that a new thick root grows on the root of the regeneration plant; the root is transplanted to nutritional soil for rejuvenation culture; and finally, the root is transplanted to a land for growing field crops to normally grow and seed. The regeneration technology is suitable for the superior corn self-bred line agriculture line 531 with high application value, can ensure that the superior quality of agriculture line 531 corn can be inherited in the corn transgenosis breeding process, and has an important meaning for functional genome group research.

Description

technical field [0001] The invention relates to a corn tissue culture regeneration technology, belongs to the field of plant genetic engineering and transgenic breeding, and relates to a method for establishing a high-efficiency regeneration system of the corn inbred line Agricultural Line 531. Background technique [0002] Corn is one of the main food and feed crops in my country, and it is also an important industrial raw material. With the continuous development of animal husbandry and processing industry, the demand for corn is bound to increase further. According to statistics, at the beginning of this century, my country's grain production demand reached 5×10 8 t, of which corn reaches 1.25×10 8 t, it is estimated that by 2030, the demand for corn in my country will reach 1.6-1.75×10 8 t. If the planting area remains unchanged, the current 4387kg / hm 2 The unit yield level is increased to 5000kg / hm 2 Left and right, this is the arduous task of maize breeding in my...

AI Technical Summary

Problems solved by technology

Although the induction efficiency of the model inbred line A188 is high, its agronomic value is very low, and it takes a long time of hybridization and selection to form new transgenic germplasm after the introduction of foreign genes; The regeneration ability of inbred lines is generally low, which cannot meet the needs of maize transgenic breeding

However, there is no research on the establishment of high-efficiency regeneration system of the excellent inbred line Nonglin 531

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