Tissue culture method of Limonium aureum

A technology of tissue culture of Limonium chrysalis, which is applied in the field of tissue culture of Limonium chrysalis, can solve the problems of environmental factors, low seedling raising efficiency, limited soil salinity, etc. The effect of improving the efficiency of asexual reproduction

Active Publication Date: 2016-07-27
GANSU DESERT CONTROL RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the preservation of Limonium chrysalis seeds requires moderate ultra-drying treatment to ensure that its vitality will not be lost; in addition, because its seeds are very small, and the thousand-grain weight is only about 0.68g, the sowing depth and water conditions must be strictly controlled during sowing; and Seed germination is also limited by soil salinity
In summary, the existing seedlings of Limonium chrysalis are easily affected by environmental factors, resulting in low seedling efficiency

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A method for tissue culture of Limonium chrysalis, which obtains regenerated shoots through a callus induction pathway, comprising the steps of:

[0022] (1) Seed disinfection: Rinse Limonium chrysalis seeds with running water for 30 minutes, then disinfect them with 75% alcohol for 10 minutes; then use 0.1% HgCl 2 Deeply sterilize the seeds for 10 minutes, and finally rinse them with sterile water for 5 times, each time for 5 minutes. After rinsing with sterile water, dry the moisture on the surface of the seeds with sterile filter paper under sterile conditions;

[0023] (2) Cultivation of sterile seedlings: inoculate the sterilized Limonium hemorrhoids seeds on the surface of MS solid medium, and culture them under light at 25°C for 25 days;

[0024] (3) Callus induction: use the cotyledons of Limonium chrysalis aseptic seedlings as explants, inoculate in callus induction medium MS+6-BA1mg L -1 +2-4D1mg L -1 , cultured at 25°C and in the dark to obtain callus tissu...

Embodiment 2

[0028] A method for tissue culture of Limonium chrysalis, which obtains regenerated shoots through a callus induction pathway, comprising the steps of:

[0029] (1) Seed disinfection: Rinse Limonium chrysalis seeds with running water for 30 minutes, then disinfect them with 75% alcohol for 10 minutes; then use 0.1% HgCl 2 Deeply sterilize the seeds for 10 minutes, and finally rinse them with sterile water for 5 times, each time for 5 minutes. After rinsing with sterile water, dry the moisture on the surface of the seeds with sterile filter paper under sterile conditions;

[0030] (2) Cultivation of sterile seedlings: inoculate the sterilized Limonium hemorrhoids seeds on the surface of MS solid medium, and culture them under light at 25°C for 25 days;

[0031] (3) Callus induction: use the cotyledons of Limonium chrysalis aseptic seedlings as explants, inoculate in callus induction medium MS+6-BA1mg L -1 +2-4D1mg L -1 , cultured at 25°C and in the dark to obtain callus tissu...

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PUM

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Abstract

The invention relates to a tissue culture method of Limonium aureum. The tissue culture method includes: obtaining regenerated seedlings through a callus inducing path and particularly includes following steps: (1), disinfecting seeds; (2), culturing sterile seedlings; (3), performing callus inducing; (4), differentiating adventive buds; (5), performing rooting culture. The tissue culture method has the advantages that the problem that Limonium aureum seedling culturing is low in efficiency and less prone to being influenced by environment factors is solved, and vegetative reproduction efficiency of high-quality germplasm resources of Limonium aureum is improved.

Description

technical field [0001] The invention relates to the technical field of plant culture, in particular to a method for tissue culture of Limonium chrysalis. Background technique [0002] Limonium japonica, also known as pine pine, golden limonium, golden spoon leaf grass, etc., is a perennial herb of the genus Limonium genus Plumbaceae, distributed in Inner Mongolia, Xinjiang, Qinghai, Gansu, Shaanxi, Shanxi and other places. It mostly grows in fixed and semi-fixed sand dunes, saline-alkali flats, lake basins, Gobi, loess slopes and the lower part of stony hillsides, and has strong drought and salt-alkali tolerance. Its calyx is golden yellow, funnel-shaped, and membranous, and it can remain unfallen for a long time in the windless season from June to September. It is one of the few wild flowers in arid desert areas and can be used as an accessory and lining flower in flower arrangements. , can also be used to make dried flowers. Moreover, its flowers can be used as medicine,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 张莹花徐先英尉秋实王方琳李德禄
Owner GANSU DESERT CONTROL RES INST
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