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Haloxylon ammodendron regenerated seedling and callus acquisition method

An acquisition method and callus technology, applied in the field of Haloxylon regenerated seedlings and callus acquisition, can solve the problems of short lifespan of Haloxylon seeds, affect the cultivation efficiency of Haloxylon seedlings, decrease of seed viability, etc., and achieve improvement of asexuality The effect on reproductive efficiency

Inactive Publication Date: 2016-08-17
GANSU DESERT CONTROL RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] But the cultivation method of Haloxylon seedlings in the prior art is seed breeding, and the life-span of Haloxylon seed is very short, and the preservation period is only about 1 year, and its seed viability will decline significantly in the second year; There is the problem of difficult rooting of Haloxylon in multi-mode propagation, which has seriously affected the cultivation efficiency of Haloxylon seedlings

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A method for obtaining regenerated seedlings of Haloxylon ammodendron, obtaining regenerated seedlings through axillary bud induction, includes the following steps:

[0030] (1) Seed disinfection: Wash the Haloxylon ammodendron seeds with running water for 30 minutes, then disinfect with 75% alcohol for 10 minutes; then use 0.1% HgCl 2 Deeply sterilize the seeds for 10 minutes, and finally rinse them with sterile water 5 times for 5 minutes each time. After rinsing with sterile water, use sterile filter paper to absorb the surface moisture of the seeds under aseptic conditions;

[0031] (2) Sterile seedling culture: inoculate sterilized Haloxylon ammodendron seeds on the surface of MS solid medium, and cultivate for 10 days at 25°C under light conditions;

[0032] (3) Axillary bud induction: The stem of a sterile Haloxylon ammodendron seedling is used as explants and inoculated into the axillary bud induction medium MS+6-BA 1.5mg·L -1 +IBA 0.5mg·L -1 , Cultivated under 25℃ and ...

Embodiment 2

[0036] A method for obtaining regenerated seedlings of Haloxylon ammodendron, obtaining regenerated seedlings through axillary bud induction, includes the following steps:

[0037] (1) Seed disinfection: Wash the Haloxylon ammodendron seeds with running water for 30 minutes, then disinfect with 75% alcohol for 10 minutes; then use 0.1% HgCl 2 Deeply sterilize the seeds for 10 minutes, and finally rinse them with sterile water 5 times for 5 minutes each time. After rinsing with sterile water, use sterile filter paper to absorb the surface moisture of the seeds under aseptic conditions;

[0038] (2) Aseptic seedling culture: inoculate the sterilized Haloxylon ammodendron seeds on the surface of the MS solid medium, and culture them for 20 days at 25°C under light conditions;

[0039] (3) Axillary bud induction: The stem of a sterile Haloxylon ammodendron seedling is used as explants and inoculated into the axillary bud induction medium MS+6-BA 1.5mg·L -1 +IBA 0.5mg·L -1 , Cultivated und...

Embodiment 3

[0043] A method for obtaining callus of Haloxylon ammodendron includes the following steps:

[0044] (1) Seed disinfection: Wash the Haloxylon ammodendron seeds with running water for 30 minutes, then disinfect with 75% alcohol for 10 minutes; then use 0.1% HgCl 2 Deeply sterilize the seeds for 10 minutes, and finally rinse them with sterile water 5 times for 5 minutes each time. After rinsing with sterile water, use sterile filter paper to absorb the surface moisture of the seeds under aseptic conditions;

[0045] (2) Sterile seedling culture: inoculate sterilized Haloxylon ammodendron seeds on the surface of MS solid medium, and cultivate for 10 days at 25°C under light conditions;

[0046] (3) Callus induction: use the cotyledon, stem tip or stem segment of a sterile Haloxylon ammodendron seedling as explants and inoculate it in callus induction medium MS+2,4-D1.0 mg·L -1 +6-BA1.0mg·L -1 , Placed in 25 ℃, dark conditions to obtain callus; induction rate of more than 90%.

[0047] (4...

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Abstract

The invention relates to a haloxylon ammodendron regenerated seedling and callus acquisition method. The method adopts an axillary bud induction method to obtain a regenerated seedling and comprises the following steps: (1) seed disinfection; (2) aseptic seedling culturing; (3) axillary bud induction; (4) strong seedling culturing; and (5) rooting culturing. The invention further discloses a haloxylon ammodendron callus acquisition method, comprising the following steps: (1) seed disinfection; (2) aseptic seedling culturing; (3) callus induction; and (4) callus proliferation. The method has the advantages that the haloxylon ammodendron vegetative propagation rooting problem is solved, the vegetative propagation efficiency of the high-quality haloxylon ammodendron seed resource is increased, and the acquisition of the haloxylon ammodendron callus can lay the early foundation for study of screening of stress resistant mutants.

Description

Technical field [0001] The present invention relates to the technical field of plant culture, in particular to a method for obtaining regenerated seedlings and callus of Haloxylon ammodendron. Background technique [0002] Haloxylon ammodendron is a sand-fixing plant that grows on sand and can also be used as fodder for livestock. The rare Chinese medicine Cistanche is parasitic on the roots of Haloxylon ammodendron. Haloxylon ammodendron belongs to the national second-level protected plant and is one of the vegetation types with the highest biological yield in the temperate desert. It is not only drought-tolerant, cold-resistant, salt-alkali resistant, wind-proof and sand-fixing, preventing land desertification, improving soil, and restoring vegetation, but also protecting the surrounding desertified grasslands and playing an incomparable role in maintaining ecological balance. [0003] However, the method for cultivating Haloxylon seedlings in the prior art is seedling cultivati...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张莹花纪永福李德禄王方琳
Owner GANSU DESERT CONTROL RES INST
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