180 results about "Actinidia arguta" patented technology

The invention discloses a variety breeding and early-season high-yield cultivation technology for actinidia arguta. The variety breeding and early-season high-yield cultivation technology includes procedures of 1 variety selection, 2 seedling propagation, 3 field planting, 4 underground management, 5 pest control and 6 pruning. Seedling propagation carried out in a step 2 includes collecting and storing cuttings and performing root cutting on the cuttings in sunlight greenhouses, particularly, root cutting is performed on the cuttings in the nutrient pots, and greenwood cutting is performed on the cuttings. Compared with the prior art, the variety breeding and early-season high-yield cultivation technology has the advantages that seedlings are raised in the sunlight greenhouses by means of root cutting, so that the seedling growth time can be prolonged, the seedlings are healthy and are high in growth potential after being planted, and a foundation is laid for early and high yields; frame surfaces are replaced by small canopy frames, and accordingly operation and management can be facilitated.

The actinidia arguta is a specific precious wild resource. The meat of the actinidia arguta is jade green; the actinidia arguta tastes fine, juicy, fragrant and delicious; the sweetness and the sourness are proper; the nutrition is very rich; the actinidia arguta is a fruit well received by customers and is known as the king of fruits. Wild actinidia arguta resources are readily available in Changbai Mountains, but products of the actinidia arguta are few and cannot meet the consumption demands in different seasons, so that the demands of the customers for wild actinidia arguta products are suppressed. The invention discloses a preparation technology for Changbai-mountain pure natural wild actinidia arguta fruit powder. According to the technology, the freeze-dried fruit powder is prepared by selecting fresh and intact actinidia arguta, washing, smashing, pulping and concentrating the actinidia arguta, adding maltodextrin and performing vacuum freeze-drying on mixed fruit paste. The actinidia arguta freeze-dried fruit powder is high in nutrient value and is convenient food suitable for various groups of people.

A composite fruit juice and its processing method belong to the deep processing field of fruits. The invention aims to solve the technical problem that lipid in present fruit juice drinks is not easy to absorb. The composite fruit juice is mainly prepared by processing blackcurrant, raspberry, cherry, sea-buckthorn, Actinidia arguta, blueberry, blackberry, lingonberry, strawberry, pomegranate, mulberry, Rosa davurica, lonicera edulis and Acanthopanax Sessiliflorus. The processing method is to adopt technologies of fermentation, alcohol extraction, crushing, mixing, ultra-high temperature sterilization and the like. The composite fruit juice has a good capability of removing hydroxyl radicals and the clearance reaches up to 96.9%.

The invention discloses a kiwi fruit InDel molecular marker and a screening method and application thereof. Two different cold-proof Actinidia arguta genome DNAs are used as research subjects, resequencing of four variety genes is carried out, InDel primers are designed according to resequencing data, and PCR (polymerase chain reaction) amplification, agarose electrophoresis and non-denaturing polyacrylamide gel electrophoresis are carried out; polymorphism detection is carried in Actinidia arguta to screen out 14 pairs of InDel primers, the 14 pairs of InDel primers are applicable to the genetic diversity analysis for Actinidia chinensis, Actinidia deliciosa and the like and are also applicable to the researches, such as hybrid offspring authenticity identification, genetic map construction, and molecule-assisted breeding. The kiwi fruit InDel primers according to the embodiment of the invention has good mutation stability and low detection difficulty, allow InDel insertion/loss of large fragments, and allows agarose analysis, with steps that may be simplified.

The invention provides a fermentation method of a taravine fruit wine. The fermentation method comprises the following steps: treating fresh taravines; pulping; clarifying the obtained juice: mixing the juice with cellulase and pectinase, namely, dissolving the cellulase and the pectinase with a small amount of the juice, adding the dissolved cellulase and pectinase to all the juice, evenly mixing, and keeping standing at the temperature of 20-25 DEG C for 4 hours, wherein, based on the weight of the juice, 30-40mg of the cellulose and 40-50mg of the pectinase are added to each kilogram of the juice; adding water and sucrose for adjustment; carrying out primary fermentation and secondary fermentation; and preserving and aging for 1 year to finally obtain the taravine wine. The fermentation method provided by the invention has the positive effects that the taravines are taken as raw materials, and the cellulase and the pectinase are mixed with the juice to obtain the fermented fruit wine, which solves the difficulties of taravines such as high pectin content and low possibility of juice extraction, opens up a new field for utilization of taravine resources in China and enriches the variety of fruit wine products in the market; and the finished wine is yellow green, clarified and transparent, has unique taravine fragrance and good palatability without abnormal taste.

The invention belongs to the fields of artificial cultivation and molecular biology of A. arguta, and particularly relates to a molecular marker for early identification of sex of A. arguta young seedlings and an application of the molecular marker. Most of A. arguta belongs to dioecious plants, the economic value of female plants is apparently higher than that of male plants, in the breeding course, the juvenile stage of tree seedlings is excessively long, and male plants and female plants can be distinguished out by naked eyes after 5-7 years, so that if the sex of the young seedlings can beidentified at the early stage, massive breeding cost can be saved. The invention provides the molecular marker for early sex identification of tree seedlings of the A. arguta, and based on the molecular marker, a reagent kit for auxiliary breeding for the sex of the A. arguta and an early identification method for the sex of the A. arguta are established. Through detection on 95 A. arguta singleplants of which the sex is known, the testing accuracy rate can reach 95.7%. The molecular marker and the method disclosed by the invention are good in generality, simple to operate, good in stabilityand good in application prospects.

The invention relates to a method for preparing polysaccharides from fruits, in particular to a method for preparing actinidia arguta polysaccharides from actinidia arguta. The actinidia arguta polysaccharides can be effectively extracted from actinidia arguta through the adoption of the method, and the method has great research significance on effect and clinical application of the actinidia arguta polysaccharides.

The invention relates to the field of plant pollen preservation and in-vitro culture vitality detection and particularly relates to a preservation and in-vitro culture vitality detection method for pollen of Actinidia arguta. The method comprises the steps: picking flower buds of the Actinidia arguta at a big bud stage and taking out anthers; drying the anthers, and carrying out screening, so as to collect pollen; sealing the collected pollen, and carrying out cryopreservation at the temperature of -85 DEG C to -75 DEG C; taking out the cryo-preserved pollen, and rewarming the cryo-preserved pollen at room temperature; uniformly spreading the rewarmed pollen onto a solid culture medium, carrying out culturing, and then, carrying out microscopy under a microscope, and counting the germinating rate of the pollen. According to the preservation method, the vitality of the pollen is still preserved good after the pollen is preserved for a long time, so that the pollen in-vitro culture detection method can be applied to the vitality detection on the pollen of the Actinidia arguta and is simple, rapid and accurate.

The invention discloses a method of cultivating Actinidia arguta, comprising the steps of selecting a variety, constructing a yard, improving soil, pruning, managing fertilizer and moisture, controlling disease and pest damage and the like; the method has the advantages that seedling cost is decreased, management cost is decreased, fertilizer utilization rate is increased, and the yield and quality of Actinidia arguta are significantly increased.

The present invention relates to a liquid compound biological preservative. The liquid compound biological preservative is characterized by consisting of the following raw materials in parts by weight: 5-25 parts of chitosan, 5-25 parts of epsilon-polylysine, 5-25 parts of lysozyme, 5-20 parts of streptomyces hygroscopicus liquid, 5-20 parts of streptomyces microflavus liquid, 5-20 parts of streptomyces aureochromogenes liquid, 5-20 parts of lactic streptococci liquid, 5-20 parts of bacillus subtilis liquid, 5-20 parts of bacillus licheniformis liquid, 5-20 parts of beer yeast liquid, 5-20 parts of paecilomyces lilacinus liquid and 5-20 parts of trichoderma harzianum liquid. The liquid compound biological preservative is high-efficient, non-toxic, healthy and nutritious, beneficial for food safety, and especially has significant effects on preservation of nanguo pears, actinidia arguta or strawberries.

The invention discloses a traditional Chinese medicine composition for treating diabetes. The traditional Chinese medicine composition is prepared from the following medicinal materials: astragalus membranaceus, codonopsis, Indian buead, Chinese yam, black beans, ginseng, soroseris umbrellata, caudate sweetleaf flower, dwarf lilyturf tuber, mongolian snakegourd root, root of rehmannia, pulp of dogwood fruit, polygonatum sibiricum, Chinese magnoliavine fruit, yam rhizome, figwort root, prepared rhizome of rehmannia, palmleaf raspberry fruit, persimmon exocarp, coptis chinensis, lalang grass rhizome, Japanese honeysuckle stem, actinidia arguta, honeysuckle flower, weeping forsythiae capsule, heartleaf houttuynia herb, sharpleaf galangal fruit, gypsum, dendrobium, conic gymnadenia tuber, trillium tschonoskii maxim, gardenia fruit, white mulberry root-bark, barbary wolfberry fruit, rhizoma alismatis, stevia rebaudiana, fragrant solomonseal rhizome, flatstem milkvetch seed, corn silk, scorched hawthorn fruit, rhizoma atractylodis, largehead atractylodes rhizome, pinellia tuber, dried orange peel, officinal magnolia bark, silkworm chrysalis, hyacinth bean, round cardamom fruit, fructus amomi, pseudo-ginseng flower, earthworm, Chinese angelica, red paeony root, szechuan lovage rhizome, safflower, peach seed, rhubarb, cassia twig, compound of glauber-salt and liquorice and giant knotweed rhizome. Proved by clinical trials, the traditional Chinese medicine composition disclosed by the invention can be used for safely and effectively treating diabetes.

The invention provides a method for detecting the maturity of actinidia arguta. The method comprises the steps of from 70-97 days after full-bloom stage, picking fruits optionally every day, measuring the basic indexes of the picked actinidia arguta in the same day, detecting the six physiological and biochemical indexes including fruit hardness, soluble solid, titratable acid, starch, tannin and fruit and seed degreening index, and correspondingly establishing four classification standards of the maturity; measuring classification standard maturity ranges of the four or more physiological and biochemical indexes of the sample to confirm grade of maturity of the fruits of the sample. After the method is adopted, the maturity of the actinidia arguta can be effectively detected, so that the best picking times of the actinidia arguta with different selling demands can be confirmed, the freshness, the color, the aroma and the taste of the fruits can be guaranteed in the subsequent storage or processing process, the demands of the market and the business can be well met, the economical benefit is increased, and powerful technical support is provided for the actinidia arguta industry.

The invention provides a method for cultivating actinidia arguta anther into haplobiont. The method comprises the following steps: (A) collecting flower buds developed by actinidia arguta microspores, carrying out low-temperature refrigeration treatment for 1-10 days, and peeling anther; and (B) putting the anther in a callus tissue induction medium for carrying out dark culture for 20 days or more, culturing the anther for 40 days or more in a plant differentiation medium, and finally transferring the anther into a rooting medium for culturing and rooting to obtain the haplobiont. Through the method for cultivating actinidia arguta anther into haplobiont of the embodiment, multiple homozygous breeding materials are provided for breeding of actinidia arguta; selection and breeding materials are provided; meanwhile, an ideal receptor material is provided for the basic theoretical research; compared with the manners such as an in-vitro microspore culturing manner and a somatic chromosome eliminating manner, the anther culturing method is simple and convenient to operate; a method of artificially culturing in-vitro anther is an effective method for obtaining haplobiont of actinidia arguta; the method is extremely worthy of extensive popularization and application.

The invention relates to a Northeast wild kiwi beverage and a preparation method thereof. The beverage comprises one or more than one of actinidia kolomikta (commonly known as kolomikta), actinidia arguta (commonly known as arguta) and actinidia polygama (commonly known as polygama). The preparation method comprises the following steps: extracting by methods of percolating, decocting, refluxing and the like through water or alcohol and other organic solvents allowed in a food production process to obtain an extracting solution; or squeezing by using a physical method to obtain raw juice; or uniformly mixing concentrated juice obtained by an enzymolysis juicing process with honey, white granulated sugar or auxiliary materials such as sucrose, fructose and a solubilizer allowed in the food production process to prepare various beverages and healthcare beverages suitable for being drunk. The beverage has the functions of resisting oxidation, ageing, fatigue, hypoxia and radiation, improving body immunity, resisting constipation, and protecting cardiocerebral vascular systems.

The present invention provides a pharmaceutical composition comprising the extract of hardy kiwifruit as an active ingredient in an effective amount to treat and prevent allergic disease and non-allergic inflammatory disease by reducing inflammation action, by inhibiting histamine release from mast cell, and by increasing the level of Th1 cytokines, IgG2a in serum and reducing the level of Th2 cytokines and IgE in serum. The present invention also provides a use of above extract for the preparation of pharmaceutical composition. The present invention also provides a health food or food additives, a cosmetic composition, a feed or feed additives comprising above extract for prevention or alleviation of allergic disease and non-allergic inflammatory disease by reducing inflammation action, by inhibiting histamine release from mast cell, and by increasing the level of Th1 cytokines, IgG2a in serum, and reducing the level of Th2 cytokines and IgE in serum.

The invention discloses Actinidia arguta jam for invigorating spleen and nourishing stomach. The jam is prepared by the following raw materials in parts by weight: 80-100 parts of Actinidia arguta, 10-20 parts of hawthorn, 3-8 parts of broccoli, 3-8 parts of carrots, 3-8 parts of cherries, 5-9 parts of Chinese chestnut powder, 5-9 parts of walnut powder, 3-7 parts of royal jelly, 8-18 parts of a traditional Chinese medicine concentrate, 2-5 parts of malic acid, 2-5 parts of xylitol, 1-5 parts of pectinase, and 1-2 parts of a thickening agent. The traditional Chinese medicine concentrate is prepared by the following raw materials in parts by weight: 3-8 parts of star anise, 1-5 parts of hawthorn, 2-4 parts of large-headed atractylodes rhizomes, 1-3 parts of white peony roots, 5-8 parts of dried orange peels, 3-5 parts of rhizoma atractylodis, 1-5 parts of codonopsis roots, 1-5 parts of sword beans, 3-7 parts of bergamot, 3-8 parts of pueraria roots, and 2-5 parts of poria cocos.

The invention discloses an actinidia arguta greenwood cutting seedling raising method, and relates to the technical field of actinidia arguta seedling raising. The method includes the following stepsthat peat and fine sand serve as a seedbed matrix, after the peat and the fine sand are mixed to be uniform, a seedbed with the height of 5-8 cm is made, and meanwhile the seedbed is completely watered with a prepared agent solution, wherein the agent solution is prepared from 3,000-4,000 ppm of IBA (indolebutyric acid), 1,000-2,000 ppm of NAA (naphthylacetic acid) and 1,000 folds of 50% carbendazim; branches with the thicknesses of 0.2-0.4 cm are collected, the collected branches are cut into cuttings with the lengths of 2-4 cm, upper notches are located at the positions 1-1.5 cm above bud eyes, one leaf is reserved, and the leaf is cut by half; the branches are inserted into the seedbed, the bud eyes are exposed out of the surface of the matrix, and the cutting density is 750-850 per square meter;after cutting is completed, the temperature is controlled at 20-28 DEG C, the air humidity is kept at 60-90%, and the photosynthetically active radiation (PAR) is kept at 10-50 umol m<-2>s<-1>.

The invention belongs to the technical field of plant extraction and analysis, and particularly relates to an actinidia arguta root extract as well as an extraction and separation method and application thereof. The extract comprises the following 21 chemical components: 21 chemical components; the invention discloses a preparation method of 7-O-[beta-D-xylose pyranosyl-(1-6)-beta-D-glucopyranoside]-1, 2, 4, 5, 6, 7-O-[beta-D-xylose pyranosyl-(1-6)-beta-D-glucopyranoside]-1. The preparation method comprises the following steps: preparing 2, 8-dihydroxy-3-methoxy-ketone; 3-O-[beta-D-rhamnose-(1-6)-beta-D-galactopyranoside]-5, 7, 3 ', 4'-tetrahydroxy flavone, catechin, beta-sterol, quercetin, isoquercitrin, oleanolic acid, ursolic acid, 2alpha, 3beta-dihydroxy oleanane-12 alkene-28 acid, 2alpha, 3alpha, 24-trihydroxy ursolic acid and the like. The extract provided by the invention has a good application prospect in preparation of anti-cancer drugs for improving the advanced living standard of cancer patients.

The invention discloses an extraction method for vitamin C in actinidia arguta, and belongs to the technical field of nutritional supplements. The method comprises the following steps: (1) cleaning actinidia arguta with boiled and cooled water; (2) adding a citric acid solution to the actinidia arguta, and then carrying out homogenate treatment by a tissue homogenizer to obtain actinidia arguta pulp; (3) adding a cellulose solution to the actinidia arguta pulp, stirring the actinidia arguta pulp in a vacuum condition at 4 DEG C for 8-10 hours to obtain a mixed solution; (4) carrying out ultra-filtration on the mixed solution by virtue of a hollow fiber filter membrane to obtain filtrate; and (5) carrying out vacuum decompression concentration on the filtrate, and then carrying out freeze drying lyophilization to obtain the vitamin C in the actinidia arguta. The extraction method for the vitamin C in the actinidia arguta is simple in technical scheme, low in cost and easy to popularize; the extraction rate is over 95%; and the purity of the final product Vc is over 48%.

The invention discloses a cultivation method for organic actinidia arguta. The cultivation method for organic actinidia arguta comprises the following steps: seed treatment, germination accelerating, culture of seedlings through a greenhouse, land selection, scientific soil preparation, transplanting in a field, field management and harvesting. In the cultivation method for organic actinidia arguta, a bio-organic fertilizer which contains quite abundant organic matters and is high in fertilizer efficiency is adopted, and has properties of increasing organic matters of soil, improving a soil structure, facilitating the activity of soil microorganisms, enhancing fertilizer retaining and fertilizer supply of soil and the like, the yield of crops is further increased obviously, and the quality of agricultural products is improved; during soil preparation, lime and a lime sulphur are fed, and have effects of preventing diseases and pests; when seedlings are transplanted into a field, an organic fertilizer with scientific formula is added and is sterilized, the growing speed of the actinidia arguta is greatly increased, pest and disease damage can further be reduced, and the yield is increased; and pests are repelled by Chinese herbal medicines, pesticide is avoided, the effect of preventing and controlling pest and diseases damage is good, furthermore, the Chinese herbal medicines are safe for natural enemies of the pests, the cultivation method has actual application value, and moreover, the purposes of high yield, safety and health of production of the actinidia arguta are achieved.