43 results about "ACTINIDIA POLYGAMA FRUIT" patented technology

This invention relates to a rapid induction culture medium and method for inducing bud formation from kiwifruit leaves to complete plants, belonging to the field of biotechnology. The callus induction culture medium for rapidly inducing bud formation from kiwifruit leaves includes MS medium, sucrose, 6-benzylaminopurine, naphthaleneacetic acid, thidiazuron, and agar, with the following weight ratios: MS medium 0.002%-0.006%, sucrose 0.01%-0.05%, 6-benzylaminopurine 0.000001%-0.000003%, naphthaleneacetic acid 0.0000002%-0.0000008%, thidiazuron 0.000001%-0.000003%, agar 0.006%-0.01%, and the balance being water. The formula for the callus induction bud culture medium is as follows: MS medium 0.002%-0.006%, sucrose 0.01%-0.05%, 6-benzylaminopurine 0.0000005%-0.000003%, naphthaleneacetic acid 0.00000005%-0.0000002%, agar 0.006%-0.01%, with the balance being water. This invention provides a rapid induction culture medium and method for kiwifruit leaves to complete plants, effectively inhibiting problems such as callus waterlogging, browning, and vitrification. It not only increases the number of buds but also improves the quality of buds, which is beneficial to the growth and differentiation of callus tissue, making it easier for it to differentiate into buds, thus laying a solid foundation for subsequent genetic transformation experiments.

The application provides a method for improving the germination rate and storage capacity of kiwi pollen, and belongs to the technical field of forest tree breeding. The method comprises the following steps: S1, placing the pollen of male kiwi flowers in 24-28 DEG C light treatment for 10-14 hours; S2, spreading the pollen treated in S1 on an in vitro germination medium for light culture; S3, observing the germination state of the pollen and calculating the survival rate of the pollen. According to the method, the pollen is first placed in 24-28 DEG C light pretreatment for 10-14 hours, so that the germination rate of the pollen is significantly higher than that of fresh pollen, and compared with the pollen without pretreatment, the method can further improve the pollen storage capacity of kiwi pollen under 4 DEG C and-20 DEG C storage conditions, and the decrease range of the pollen germination rate during storage is significantly reduced.

This invention provides a Psa disease resistance gene and its application. Specifically, this invention demonstrates through genetic and molecular experiments that the Psa disease resistance gene is effective against the hardy kiwifruit (Actinidia arguta). Actinidia arguta Wild germplasm Aa11251 containing the G932 protein sequence triggered an immune response upon injection of the HopAO2 effector protein from *Actinidia chinensis*, while materials lacking the G932 protein did not. Transient co-expression experiments on the model plant *Nicotiana benthamiana* showed that co-expression of the resistance protein G932 and the effector protein triggered an HR immune response in the plant. Simultaneously, in *Actinidia chinensis* (Chinese kiwifruit...),... A. chinensis The stable expression of G932 in the transgenic 'Donghong' variety significantly enhances its resistance to pathogens. This indicates that the G932 protein and its encoding gene can be used in agricultural production for the breeding of resistant kiwifruit varieties to bacterial canker. This invention is of great significance for the breeding of disease-resistant kiwifruit varieties and can be used in agricultural production.

A transcription factor for regulating resistance of kiwifruit to botrytis blight AcWRKY84 The application relates to a gene, and after the expression of the gene is silenced, the resistance of kiwifruit to botrytis blight is significantly reduced. AcWRKY84 The application clones the gene and proves the role of the gene in resisting botrytis blight in kiwifruit, and specifically, after the experimental group is inoculated with the virus, the disease symptoms of the experimental group are more serious, the infected area is obviously increased, and the SOD, POD and CAT enzyme activities in the fruit are significantly reduced compared with those of the control group, so that sufficient gene resources can be provided for a botrytis blight resistance gene library, and the possibility of realizing successful breeding of a high botrytis blight resistance variety is greatly increased.

The application discloses a ubiquitin transporter AcRAD23D1, a coding gene and application thereof, and belongs to the technical field of genetic engineering. The application clones an AcRAD23 family gene AcRAD23D1 from a kiwi fruit, and compares phenotypes and determines physiological parameters under transgenic and drought stress treatment, and identifies biological functions of the gene in positive regulation of drought resistance of the kiwi fruit in detail. The research result has important theoretical significance and practical application value for carrying out molecular directional breeding of the kiwi fruit. The overexpression transgenic kiwi fruit plant obtained by using the agrobacterium-mediated maceration method has significantly increased drought stress resistance, the transgenic kiwi fruit plant with silenced expression has significantly reduced drought stress resistance, and the application has important application value in molecular directional breeding of the kiwi fruit and reduction of cultivation environment limitation.

This invention relates to a Bacillus tequilensis microbial agent, its preparation method, and its application, belonging to the field of agricultural microbiology. A Bacillus tequilensis YP131 microbial agent, with accession number CGMCC No. 26340, was deposited at the China General Microbiological Culture Collection Center on December 27, 2022. The Bacillus tequilensis YP131 (CGMCC No. 26340) microbial agent of this invention can significantly reduce the occurrence of summer canker in kiwifruit. Spraying with the Bacillus tequilensis YP131 microbial agent showed a control efficacy of 75.01% against summer canker in kiwifruit.

The present application relates to the technical field of plant molecular biology, and proposes a kiwi drought-resistant gene AcNFXL1 and application thereof.A kiwi drought-resistant gene AcNFXL1 The nucleotide sequence of the kiwi drought-resistant gene AcNFXL1 is shown as SEQ ID NO.1.Through the above technical solution, the problem of poor drought resistance of kiwi in the prior art is solved.

The utility model relates to the technical field of kiwi fruit planting, and disclose a kind of climbing frame for kiwi fruit planting, including two support plates, the outside of two support plates is slidably provided with a plurality of mounting plates, the inside of a plurality of mounting plates is slidably penetrated with first fixed bolt and first fixed bolt penetrates support plate, the top of a plurality of mounting plates is fixedly provided with two clamping plates, same horizontal two mounting plate top is connected with fixed plate and fixed plate is between two clamping plates, second fixed bolt is slidably penetrated between two clamping plates and second fixed bolt penetrates fixed plate, when planting, by supporting structure, support plate is installed in suitable position, then according to actual demand, a plurality of mounting plates are slidably installed on the outside of support plate, fixed by first fixed bolt, then fixed plate is installed between the top of same horizontal two mounting plates, fixed plate is fixed, to adjust and increase fixed plate according to use requirement, facilitate kiwi fruit to climb.

This invention discloses the application of the natural product santalol as an agricultural fungicide in plant disease control, belonging to the field of plant disease prevention and control. It shows good inhibitory effects against bacterial diseases such as rice bacterial leaf blight, rice bacterial leaf streak, citrus canker, cabbage black rot, tomato bacterial spot, kiwifruit canker, tobacco bacterial wilt, cucumber bacterial angular leaf spot, and potato bacterial wilt. It also shows good inhibitory effects against viral diseases such as tobacco mosaic virus; and against fungal diseases such as rice rhizome blight, wheat scab, pepper wilt, *Botrytis cinerea*, or *Colletotrichum sorghum*. It can be used in the preparation of plant fungicides. Santalol is a natural plant extract and is environmentally friendly. This invention provides a new use for the natural product santalol, enabling its application in the agricultural field for plant disease control and providing a new avenue for the research and development of new pesticides.

1. The name of the design product: kiwi fruit packaging box. 2. The use of the design product: for packaging kiwi fruit. 3. The design points of the design product: in the combination of shape and pattern. 4. The picture or photo that best shows the design points: front view. 5. The bottom surface of the design product is the part that is not easy to see or cannot be seen during use, so the top view is omitted.

This invention discloses a pectin-nanolignin-citrus essential oil composite coating liquid, its preparation method, and its application. The preparation method includes the following steps: first, a nanolignin suspension is prepared; then, citrus essential oil is added and homogenized to obtain a nanolignin Pickering emulsion; finally, a pectin solution is added and homogenized again to obtain the pectin-nanolignin-citrus essential oil composite coating liquid. When used for food preservation, the pectin-nanolignin-citrus essential oil composite coating liquid of this invention forms a composite coating with excellent broad-spectrum antibacterial, antioxidant, UV-resistant, and long-term essential oil release properties. It also enhances physical properties such as mechanical properties, water vapor / oxygen barrier properties, and hydrophobicity, significantly extending the shelf life of climacteric fruits (kiwifruit) and non-climacteric fruits (strawberries), demonstrating great application potential in the field of fruit preservation.

A method for agricultural cultivation, domestication, or cultivation of truffle fungi, including a method for isolation and culture to produce pure truffle fungi or mini-truffles, typically species of the genus Tuber or Iamai. Examples of truffles include Tuber melanosporum, Tuber magnatum, Tuber aestivum, Tuber uncinatum, Tuber borchii, species of the genus Imaia, Tuber macrosporum, Tuber gibbosum, Tuber oregonense, and Tuberlyonii. The isolated pure truffles may then be cultured on a nutrient substrate, which may be, for example, fruits, nuts, grains, or parts thereof, to produce truffle-flavored foods. Non-limiting examples of such substrates include rye, barley, lentils, wheat, rice, soybeans, pecans, hazelnuts, pine nuts, walnuts, coffee beans, mustard, cocoa, sesame, sunflowers, grapes, blackberries, blueberries, cherries, kiwis, mangoes, raspberries, and huckleberries.

The present application belongs to the field of microbial technology, and discloses a compost bacillus DX-10 and application thereof. The present application provides a compost bacillus, which is preserved in China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 20251073. The strain has a good prevention and treatment effect on kiwi soft rot, and shows excellent inhibition on the pathogenic bacteria of kiwi soft rot, such as grape Coniothyrium and Diaporthe phaseolorum. The strain provides a strain resource for preparing a biocontrol agent, can alleviate the environmental hazards caused by excessive use of chemical agents, and is expected to become an important tool for developing green control drugs for kiwi soft rot.

The utility model discloses a kind of climbing frames for kiwi planting, it is related to kiwi field, including main support rod, the bottom end of main support rod is butt joint with inserting nail body, the side of main support rod is evenly provided with side screw hole, the top end of main support rod is butt joint with butt joint top block, the side of butt joint top block is symmetrically provided with mounting screw hole, the side of butt joint top block is butt joint with connection top rod horizontally, the side of main support rod is evenly butt joint with auxiliary sleeve, one end of auxiliary sleeve is inserted with extension end rod horizontally, the top end of main support rod is provided with top joint hole, the inside of top joint hole is symmetrically provided with buckling inner groove, the bottom end of main support rod is provided with bottom screw hole, the top end of inserting nail body is fixedly welded with mounting screw rod, using split structure makes it convenient to carry transport operation after disassembly, simultaneously in the fixed connection mode of buckle and thread let it be more convenient and efficient to assemble.

The application discloses a kind of active substances for crop protection and its composition and preparation method and application, the substance general formula as follows: Wherein: R1 It is hydrogen atom, halogen, cyano, methyl, methoxy, nitro, trifluoromethyl or the disubstituted of arbitrary combination of above halogen;R2 It is hydrogen atom, halogen or nitro substitution.This application derivative shows better inhibitory effect to rice bacterial leaf spot, rice bacterial leaf blight, kiwi bacterial canker, citrus canker.The inhibitory activity of compound II-18 to kiwi bacterial canker bacteria is best.Plant pathogenic fungi (sclerotinia sclerotiorum, phomopsis viticola, botryosphaeria dothidea and fusarium graminearum) inhibitory activity test shows that compound II-10 shows better inhibitory activity to fusarium graminearum.In addition, part of the derivatives of the application has significant contact toxicity to bactrocera dorsalis (Hendel).The composition of compound II-5 and methyl eugenol shows synergistic trapping effect in the process of preventing and treating the main pest of kiwi, bactrocera dorsalis (Hendel).

This utility model discloses a kiwifruit harvesting structure, including a telescopic support rod, a motor switch and a battery on the telescopic support rod, a ring-shaped mounting base fixed at the top, a fruit receiving hopper at the bottom of the ring-shaped mounting base with a notch at the top, a cutter movable at the notch via a bearing, a motor mounted at the top, the motor output shaft connected to a crank, the crank connected to the cutter via a pull rod, the cutter reciprocating at the notch with its trajectory passing above the inner opening. The telescopic support rod is a multi-stage sleeve type, the battery is a rechargeable lithium battery with a protective shell, the handle has an anti-slip sleeve, the inner wall of the fruit receiving hopper has a flexible buffer layer, and the notch angle is 60°-120°. This utility model has a pruning mechanism at the top of the receiving mechanism, which first covers the fruit and then cuts the branch, solving the problems of inconvenience and low efficiency caused by the separate setting of the pruning and receiving mechanisms in traditional equipment, improving the convenience and efficiency of harvesting, protecting the fruit from damage, and is suitable for agricultural production.

The present application belongs to the technical field of postharvest processing of fruits, and particularly relates to a method for brightening the surface of kiwifruit based on dynamic chlorine dioxide and a processing system. The method comprises placing postharvest kiwifruit in a closed lightproof processing space, carrying out gas phase chlorine dioxide fumigation under the condition of 20-30 DEG C, and making the gaseous chlorine dioxide concentration in the processing space change according to a three-stage dynamic concentration-time curve of "rising-stable-declining". In the method, the initial concentration Co gradually rises to the peak concentration Cmax in 0-5 h, is maintained at 0.8Cmax-1.2Cmax in 5-8 h, and falls to the terminal concentration Cend in 8-12 h, wherein Co is 0.3-0.7 mg / L, Cmax is 0.7-1.1 mg / L, and Cend is 0.3-0.7 mg / L. After the processing, ventilation is carried out until the residual chlorine dioxide concentration is not higher than 0.1 mg / L. The method can improve the brightness of the fruit skin, reduce the browning index, and delay the re-browning of the fruit surface during the shelf life, under the condition of keeping the microstructure of the fruit skin cells, pores and cuticle intact, and is suitable for improving the commercial fruit surface quality of postharvest kiwifruit.

The present application belongs to the field of kiwi fruit planting, and discloses a management method of kiwi fruit trees. The present application adopts a double-layer reverse U-shaped ring cutting method, which can slow down the downward transportation of photosynthetic products to a large extent and for a long time, effectively improves the yield and quality of kiwi fruit, reduces the risk of tree death, and balances the vegetative growth and reproductive growth of kiwi fruit. In addition, the present application provides a new type of composite control agent for brown spot disease and leafhoppers, which can more efficiently and scientifically control diseases and pests, thereby significantly improving the yield and quality of kiwi fruit.

This invention provides a method for rapid propagation of seedlings from drought-resistant kiwifruit rootstock seeds, comprising the following steps: S1, selecting individual plants that still exhibit excellent performance after 20-30 days without watering at 32-37℃, extracting the seeds from the fruit, and drying them for later use; S2, soaking the seeds in a 0.15mg / L gibberellin solution for 24-48 hours, then rinsing and disinfecting them; S3, culturing the disinfected seeds in a culture medium until seedlings emerge, followed by acclimatization and transplanting. Kiwifruit rootstocks cultivated using this method maintain well-developed root systems, vigorous growth, and do not wilt or die even after 20-30 days without watering during the hot summer months. This significantly promotes the kiwifruit industry in kiwifruit-growing areas with poor soil and irrigation conditions, thereby improving economic benefits.

This invention discloses an indentation-type soft and hardness measurement device and method for identifying the maturity of post-ripening fruits. The device includes a shell, a fixed ring, a drive mechanism, an indentation self-locking mechanism, a sensing module, and a control module. The drive mechanism is installed between the shell and the fixed ring, the indentation self-locking mechanism and the sensing module are placed below the fixed ring, and the control module is installed on the inner wall of the shell. The input end of the control module is connected to a five-channel bridge circuit of the sensing module, and the output end of the control module is connected to a two-phase four-wire motor of the drive mechanism. The indentation-type soft and hardness measurement device of this invention can quantify the soft and hardness information of post-ripening fruits and judge the maturity of post-ripening fruits at different stages of maturity. Moreover, the probe in the device of this invention is made by micron-level 3D printing, which helps to identify the maturity of fruits with slight and non-destructive indentation. It is expected to be applied to the intelligent identification of post-ripening fruits such as kiwifruit and green mango.

The application provides raw Xuan paper and a preparation method thereof. The raw Xuan paper is prepared by using broussonetia papyrifera pulp and reed canary grass pulp as main materials, and adding a small amount of artemisia argyi pulp, a small amount of aerogel silicon dioxide and actinidia kiwi juice, and further spraying a cationic quaternary ammonium cellulose material on the surface of the paper. The raw Xuan paper prepared by the application has excellent insect resistance and bacterial corrosion resistance, and good oxidation resistance, so that the paper can be stored for a long time and is not easy to discolor. Meanwhile, the paper has small deformation and good shape stability after being soaked in water, and is not easy to stretch and deform. The raw Xuan paper prepared by the application can be used for calligraphy, painting, stele rubbing and mounting, and meets the requirements of good ink wetting performance, uniform ink diffusion, clear ink color gradation and clear level, good toughness, folding resistance and tensile resistance, and the like. Moreover, the preparation method is simple, the conditions are mild, the raw materials are abundant and easy to obtain, and the application is more conducive to industrialized production and popularization and application.

The present application provides a kind of kiwi fish bone shape pruning method, belong to the technical field of kiwi cultivation management.The method includes the directional cutting of the blade edge cutting into the inclined angle of 30° to 60° on the permanent main vine of the mother branch to be updated during the dormant period;Remove redundant sprouts in spring;After the target new shoot enters the initial stage of lignification and color change, implement the angle binding, so that the base forms a horizontal angle of 60° to 120°.During the growing season, summer pruning and phased water and fertilizer management are carried out on the target new shoot.The present application forms process control in the direction regulation of callus growth, wound liquid water diversion and lignin induction deposition at the base of new shoots, eliminates the conditions for abnormal shedding of new shoots and longitudinal migration of pathogens, and ensures the long-term health of the plant and the continuous stability of the structure.

The application discloses a Trichoderma guepinii GUDJ16 and application thereof, and belongs to the technical field of microorganisms. The preservation number of the Trichoderma guepinii GUDJ16 screened by the application is CCTCC NO: M 20252004. The Trichoderma guepinii GUDJ16 has a prevention and treatment effect on kiwi soft rot, and the inhibition rate of the Trichoderma guepinii GUDJ16 on Botryosphaeria dothidea can reach 77.15%. The Trichoderma guepinii GUDJ16 also has an inhibition effect on Fusarium solani, Fusarium graminearum, Fusarium asiaticum, Rhizoctonia solani, Sclerotium rolfsii, Colletotrichum musae, Passalora fulva and Alternaria tenuissima. It is found through disease prevention and treatment effect verification on in-vitro fruits infected with kiwi soft rot that the prevention and treatment effect of the Trichoderma guepinii GUDJ16 on kiwi soft rot can reach 61.26% after treatment, and therefore the Trichoderma guepinii GUDJ16 has high application value in the field of kiwi soft rot prevention and treatment.

This invention discloses a *Rummeliibacillus* sp. and its application, relating to the field of microbial technology. The *Rummeliibacillus* sp., named *Rummeliibacillus* JY-2-4R, was deposited on February 11, 2026, at the Guangdong Provincial Microbial Culture Collection Center, located at 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou, with accession number GDMCC No: 67853. This invention applies the strain to the preparation of preparations antagonistic to *Actinidia chinensis* black spot, *Actinidia chinensis* soft rot, *Actinidia arvensis* white rot, *Actinidia arvensis* soft rot, or *Actinidia arvensis* root rot. The *Rummeliibacillus* JY-2-4R of this invention is a potential biological agent possessing stress resistance, growth promotion, and broad-spectrum antibacterial activity, with broad development potential and good application prospects, addressing the problem of a lack of functional microorganisms for the biological control of kiwifruit and *Actinidia arvensis* diseases.

The utility model discloses a melon and fruit paring knife which comprises a handle, a first adjusting assembly is arranged on the handle, a second adjusting assembly is arranged on the first adjusting assembly, and by arranging the first adjusting assembly and the second adjusting assembly, adjustment can be carried out according to the use habits of the left hand and the right hand of a user. The peeler is simple in structure and convenient to use, a left hand user and a right hand user can conveniently use the peeler, the holding mode does not need to be additionally adjusted, the use comfort and convenience are improved, meanwhile, the peeler can adapt to food materials with different hardness, larger force is provided when the peeler is used for peeling potato and other hard-skin food materials, pulp damage is avoided when kiwi fruit and other soft-skin food materials are treated, and the peeler is suitable for being used for peeler processing. Therefore, the application range of the paring knife is expanded, and diversified requirements of users in different scenes are met.

The application provides a Pantoea agglomerans KVBP1 strain, which is classified and named as Pantoea agglomerans KVBP1, and is preserved in the China Center for Type Culture Collection with a preservation number of CCTCC NO: M 20252888. The application also provides application of the KVBP1 strain in prevention and treatment of kiwi fruit canker disease and a biocontrol agent for preventing and treating kiwi fruit canker disease. The application has the advantages that a strain with a significant prevention and treatment effect on kiwi fruit canker disease is screened from kiwi fruit plant tissues and rhizosphere soil, is identified as the Pantoea agglomerans KVBP1 through multi-gene sequence analysis, and is verified in function through physiological and biochemical determination and in-vitro and in-vivo prevention and treatment effect tests, thereby providing a new high-quality biocontrol strain resource and technical support for healthy and sustainable development of the kiwi fruit industry.

This invention belongs to the field of food processing technology, specifically disclosing a sleep-improving active peptide complex, its preparation method, and its application. The preparation method includes the following steps: S1, mixing and grinding jujube seed and lily bulb, sieving, mixing with kale powder, adding deionized water, heating under reflux for extraction, combining the extracts, filtering, and concentrating under reduced pressure to obtain extract A; S2, mixing dried tangerine peel, hawthorn, and kiwi fruit powder, adding a 70% (w / w) ethanol solution, heating under reflux for extraction, combining the extracts, filtering, and concentrating under reduced pressure to obtain extract B; S3, mixing extract A obtained in S1 with extract B obtained in S2, inoculating with *Lactobacillus plantarum*, fermenting to obtain a reactant; S4, mixing the reactant obtained in S3 with collagen cyclic dipeptide, spray drying to obtain the active peptide complex. This active peptide complex has good stability and solubility, can significantly shorten sleep latency, prolong sleep duration, and improve sleep quality.

The application discloses a kiwi fruit storage period deterioration monitoring method based on gas component analysis, and particularly relates to the field of kiwi fruit storage period deterioration monitoring, which comprises the following steps: introducing a mixed gas flow path into a kiwi fruit storage space, guiding the mixed gas into the entrance of an electric field trapping cavity, and keeping the mixed gas entering the electric field trapping cavity in the original component proportion of the storage space; applying a preset electric potential in the electric field trapping cavity, forming a trapping area of polar molecules in the electric field trapping cavity, and adsorbing the deterioration precursor gas in the mixed gas in the trapping area to form an enrichment structure corresponding to the adsorption time. Through the electric field trapping and spatial enrichment of the extremely low concentration of the deterioration precursor gas in the mixed gas, and then inputting the enriched high local concentration gas into a tunable laser absorption link to realize the explicitness of the weak signal, the problem that trace precursor gas characteristic signals cannot be separated in a wide absorption background in the prior art is solved.

The utility model relates to a net format steel pipe columnar shed frame for kiwi fruit planting belongs to kiwi fruit planting technical field, the net format steel pipe columnar shed frame for kiwi fruit planting, including a plurality of stand, a plurality of stand are grid -like setting, support mechanism, support mechanism set up between multiple left and right stand, the support mechanism includes crosspiece and threading ring, crosspiece respectively set up between two groups of stand, the net format steel pipe columnar shed frame for kiwi fruit planting, through being provided with adjustable threading ring above crosspiece, when the worker installs threading ring, through bolt, the clamping piece outside crosspiece is folded to crosspiece and is clamped tightly, because the top side wall of clamping piece does not contact each other, the worker uses bolt to pass through fixed hole, makes two sets of clamping piece extrude crosspiece, realizes fixed, conversely then loosens bolt, moves the position of inner sliding block, namely realizes the demand of adjusting the position of threading ring.

This invention provides a method for cultivating grafted kiwifruit seedlings with two root systems, comprising the following steps: S1, Rootstock acquisition: Select one one-year-old *Actinidia cuspidatum* and one *Actinidia deliciosa* seedling as rootstocks, and prune the main roots of both rootstocks to a length of 10-15 cm, retaining 4-5 cm of stem; S2, Scion acquisition: Select branches of the *Actinidia deliciosa* variety as scions, with a length of 8-12 cm. Seal the upper end with wax or wound healing agent, and smooth the lower end with a flat cut; S3, Grafting: Using bark grafting or cleft grafting, graft the two rootstocks in opposite directions onto the lower end of the same scion, and wrap the grafting area with grafting film to form a grafted kiwifruit seedling. The grafted seedlings cultivated by this invention have two sets of root systems, one for *Actinidia cuspidatum* and one for *Actinidia deliciosa*, thus exhibiting strong resistance to waterlogging, drought, and pests and diseases, and absorbing nutrients comprehensively, resulting in high-quality and high-yield fruit with significant economic benefits.