801 results about "ACTINIDIA POLYGAMA FRUIT" patented technology

The invention belongs to the field of gene engineering, and relates to a kiwi fruit AcERF56 gene and application thereof in drought resistance and growth improvement. The nucleotide sequence of the AcERF56 gene of the kiwi fruit disclosed by the invention is as shown in SEQ ID NO: 1; the amino acid sequence of the protein coded by the AcERF56 gene is as shown in SEQ ID NO: 2. The drought resistance function of the gene is reported in the kiwi fruit for the first time, the photosynthetic performance, overground and underground biomass and active oxygen scavenging capacity of the kiwi fruit under the water stress condition can be remarkably improved by overexpressing the gene, the drought resistance of the kiwi fruit is improved, and a research basis is provided for cultivation of drought-resistant and high-yield plants.

The invention belongs to the technical field of microbial agents, and particularly relates to a preservative and fresh-keeping microbial composition as well as a microbial agent and application thereof. The microbial composition disclosed by the invention comprises bacillus subtilis LS and bacillus velezensis LX, and in the microbial composition, the concentration ratio of the bacillus subtilis LS to the bacillus velezensis LX is (0.1-1): (0.1-1); the preservation number of the bacillus subtilis LS is CGMCC (China General Microbiological Culture Collection Center) NO.30953; the preservation number of the bacillus velezensis LX is CGMCC (China General Microbiological Culture Collection Center) NO. 30954. In the microbial composition provided by the invention, the bacillus subtilis LS and the bacillus velezensis LX can generate a synergistic effect and can be used for preventing and treating the soft rot of the kiwi fruits, and a theoretical basis and technical guidance are provided for biological prevention and treatment and storage and fresh-keeping of the soft rot of the kiwi fruits.

The invention discloses Delftia tsuhuensis and an application of the Delftia tsuhuensis in prevention and treatment of plant diseases. The Delftia tsuhuensis CDJ2 is preserved in the Guangdong Microbial Culture Collection Center on October 18, 2024, and the preservation number is GDMCC (China General Microbiological Culture Collection Center) NO: 65290. The strain has a good prevention and treatment effect on citrus canker, and the prevention and treatment effect can reach 84%. Meanwhile, the strain can also effectively prevent and treat soft rot of taro, bacterial basal rot of rice, bacterial wilt of crops, kiwifruit canker, angular leaf spot of mango, black rot of brassicaceous vegetables, tomato scab, gummosis of sugarcane, bacterial wilt of walnuts and bacterial leaf spot of plums. Wide application prospects are realized in the aspects of preventing and treating diseases of crops and commercial crops and promoting healthy growth of plants.

The invention discloses a selenium-rich bimorphic actinidia arguta enzyme as well as a preparation method and application thereof, and belongs to the technical field of microbial fermentation. The preparation method of the selenium-rich bimorphic actinidia arguta enzyme comprises the following steps: (1) performing selenium-rich culture on saussurea involucrata to obtain selenium-rich saussurea involucrata; (2) pulping the whole actinidia arguta fruits, performing enzymolysis, inoculating the selenium-rich saussurea involucrata bacteria, and performing fermentation culture; and (3) after the fermentation culture is finished, carrying out solid-liquid separation to obtain the selenium-rich solid-state actinidia arguta enzyme and the selenium-rich liquid-state actinidia arguta enzyme. The selenium-rich saussurea involucrata is used for whole-fruit fermentation of actinidia arguta for the first time, a bimorphic enzyme product is developed, the selenium content in the actinidia arguta enzyme is increased, and the enzyme flavor and antioxidant activity are enhanced. Compared with the prior art, the method has originality in the aspects of fermentation strains, culture modes, product forms and biological functions, and a whole set of processing technology which is simple in process and high in operability is provided.

The invention discloses bacillus tequilensis P61 and application thereof in prevention and treatment of kiwi fruit soft rot, and belongs to the field of biological prevention and treatment. The bacillus tequilensis P61 provided by the invention is preserved in the China Center for Type Culture Collection on September 5, 2024, and the preservation number of the bacillus tequilensis P61 is CCTCC (China Center for Type Culture Collection) NO: M 20241916. According to the invention, the lipopeptide antibacterial substance is prepared according to the bacillus tequilensis P61. Experimental results show that the bacillus tequilensis P61 and lipopeptide antibacterial substances have an outstanding prevention and treatment effect on the soft rot of the kiwi fruits, have a remarkable bacteriostasis effect on pathogenic bacteria of the soft rot of the kiwi fruits such as botryosphaeria dothidea, alternaria alternata and phomopsis, remarkably reduce the damage rate of the kiwi fruits, are green and safe, are harmless to people and livestock, and are worthy of popularization and application. No pollution is caused to the environment. The invention provides a new biocontrol strain for biological prevention and control of kiwi fruit diseases, and has a wide application prospect.

The invention discloses a postharvest variable-temperature storage method for kiwi fruits, which relates to the technical field of fruit and vegetable preservation and comprises the following steps of fruit picking, warehousing and pre-cooling, variable-temperature storage and ventilation. According to the method, stable ripening of the kiwi fruits can be achieved through variable-temperature treatment, the problem of fruit quality easily caused by a traditional chemical ripening method is avoided, the kiwi fruits ripened through the method are uniform in after-ripening, bright in color and luster and excellent in taste, the fruit quality is remarkably improved, the fresh-keeping period of the kiwi fruits is further remarkably prolonged, and the kiwi fruits are suitable for large-scale popularization and application. According to the method, the loss of the kiwi fruits in the postharvest treatment and storage process can be remarkably reduced through scientific temperature-variable ripening and storage management, and the economic benefit is improved.

The invention discloses a preparation method of a fruit and vegetable juice beverage fermented by various probiotics in the field of food processing. The preparation method comprises the following steps: putting fruit and vegetable raw materials into auxiliary equipment, and performing high-pressure washing; the fruit and vegetable raw materials comprise cucumbers, dragon fruits, kiwi fruits and bananas; bifidobacterium, lactobacillus acidophilus, lactobacillus casei and lactobacillus plantarum are prepared and prepared into bacterial suspensions respectively, and then the bacterial suspensions are mixed in proportion to obtain a composite bacterial solution; cutting fruit and vegetable raw materials into blocks, performing low-pressure cooking, performing rapid cooling, performing juicing, and performing filtration so as to obtain fruit and vegetable juice raw materials; adding water and fructo-oligosaccharide into the fruit and vegetable juice raw material to form a fermentation base material, and then inoculating the composite bacteria liquid for fermentation to form a mixed raw material; and finally, storing to form the fruit and vegetable juice beverage. The preparation method of the fruit and vegetable juice beverage fermented by the various probiotics can adjust conditions and environments required by fermentation of the probiotics. Therefore, the fermented bean curd has the advantages of high nutritional value, good taste, sanitary preparation process, controllable fermentation process, reasonable storage mode and wide application range.

The invention provides a kiwi fruit transcription factor and application thereof in kiwi fruit canker, and belongs to the technical field of genetic engineering. The invention provides a transcription factor AcWRKY50, the nucleotide sequence of the transcription factor AcWRKY50 is shown as SEQ ID No.1, and the AcWRKY50 is positioned in a cell nucleus and has transcription activation capability. The AcWRKY50 disclosed by the invention can continuously respond to Psa infection, after Psa pathogenic bacteria infection, the expression of the AcWRKY50 gene is rapidly activated and continuously up-regulated, and after the AcWRKY50 gene is over-expressed, the resistance to Psa can be improved. Meanwhile, MeJA negatively regulates the resistance of the kiwi fruit to the Psa, and the kiwi fruit germplasm can be innovated based on the transcription factor disclosed by the invention.

The invention belongs to the technical field of genetic engineering, and relates to application of dihydroxy acid dehydratase AcDHAD in improvement of disease resistance of kiwi fruits. The invention discloses a dihydroxy acid dehydratase AcDHAD gene and a dihydroxy acid dehydratase AcDHAD protein coded by the dihydroxy acid dehydratase AcDHAD gene through excavation and functional verification, and confirms the broad-spectrum disease-resistant potential of the dihydroxy acid dehydratase AcDHAD in disease-resistant breeding of kiwi fruits. According to the invention, more gene resources are provided for molecular breeding of disease-resistant varieties of kiwi fruits, so that more opinions and ideas are provided for disease-resistant breeding of kiwi fruits and development of molecular markers.

The utility model discloses a kiwi fruit orchard meteorological disaster monitoring and processing device, and relates to the technical field of agricultural product planting. According to the technical key points, the device comprises a vertical rod, a mounting rack is arranged right above the vertical rod, a first weather shutter box is mounted on the mounting rack, and a temperature sensor is integrally mounted in the first weather shutter box; the mounting frame and the vertical rod are connected through a plurality of supporting rods; a first box body is arranged near the lower end of the vertical rod, a rotary spray head is installed at the upper end of the vertical rod, the first box body and the rotary spray head are connected through a pumping unit, and the pumping unit can adjust the working state of the pumping unit according to the environment temperature detected by a temperature sensor in the first weather louver box. When the environment temperature is monitored to be abnormal, the pumping unit can be controlled to perform temporary protection by utilizing the medicament in the first box body, so that the risk that kiwi fruits are damaged in abnormal weather is reduced, and the benefits of the kiwi fruits are ensured.

The invention discloses a special organic fertilizer for kiwi fruits and a preparation method thereof. The organic fertilizer comprises the following raw materials in parts by weight: 50-70 parts of livestock and poultry manure, 30-50 parts of kiwi fruit branch and leaf residues, 10-20 parts of soybean meal, 5-10 parts of bone meal, 3-8 parts of humic acid and 1-3 parts of a microbial agent. The microbial agent comprises bacillus subtilis, bacillus mucilaginosus, saccharomycetes and lactic acid bacteria in a ratio of 1: 1: 1: 1. The raw materials are pretreated and then subjected to a mixed fermentation process to prepare the finished product. The organic fertilizer is comprehensive in nutrition, can meet the nutritional requirements of kiwi fruit growth, improves the soil environment, improves the fruit quality and yield, and has remarkable economic benefits and environmental benefits.

The invention discloses a preparation method of a quinoa-fruit compound beverage, and belongs to the technical field of beverage processing. Comprising the following steps: S1, raw material pretreatment (in the quinoa pretreatment, high-viscosity enzymolysis modified quinoa pulp is prepared through double-enzyme synergistic hydrolysis of acid protease and natural kiwi fruit protease in kiwi fruit juice in combination with gradient heating and grinding; the preparation method comprises the following steps: pretreating raspberries, namely preparing suspended raspberry seed pulp by freeze-drying, superfine grinding and coating with enzymolysis modified quinoa pulp, and meanwhile, adding tea polyphenol, glycerol and a compound buffer agent to prepare color-protecting anti-crosslinking raspberry juice), S2, blending and homogenizing, and S3, sterilizing and filling. Through three stages of raw material pretreatment, blending and homogenization, and sterilization and filling, the nutritional conflict problems mentioned in the background technology, such as quinoa protein acid denaturation and aggregation, quinoa PPO-mediated raspberry anthocyanin oxidation browning, raspberry seed retention and suspension stability, and taste adaptation, are solved.

The invention belongs to the technical field of fruit and vegetable disease detection and identification, and relates to a kiwi fruit soft rot volatile biomarker identification method and a soft rot identification method, the identification method comprises the following steps: S1, selecting kiwi fruit for disinfection and sterilization; s2, inoculating pathogenic fungi: dividing the kiwi fruits into a healthy control group and a germ inoculating group; sterile water is inoculated to the healthy control group, and pathogenic fungi causing soft rot are inoculated to the germ inoculation group; healthy control samples and germ samples are randomly extracted every day during storage; s3, using a GC-IMS analyzer for the extracted sample to obtain a three-dimensional spectrogram of the volatile components; and S4, analyzing and identifying the volatile biomarker through VOCal software. According to the method, the volatile biomarker of the soft rot of the kiwi fruit is identified, and then whether the soft rot of the kiwi fruit is generated or not is identified through the identified volatile biomarker, so that the method has the characteristics of rapidness, accuracy, sensitivity and no damage, and monitoring and early warning of the soft rot of the kiwi fruit can be realized.

The present invention relates to a method of intensive and sustainable cultivation for cultivating fruit trees with high water consumption, such as avocado trees, through the multivariate management of: plant structure, nutrients, dynamic density, artificial pollination, and the use of specific wavelengths for each phenological state that allows the control of early transition to production, maximum yield per surface area unit, and fruit harvest time. A method for cultivating fruit trees with high water consumption (such as avocado, citrus, apple, mango, pear, peach, kiwi trees, among others) in containers for intensive fruit production comprises the steps of placing avocado tree plants with a high absolute density in aerated containers, placing in the containers a substrate for accelerated root growth, hydroponically cultivating the containers with a nutrient solution that provides early plant emergence, flowering, and fruiting, and placing the containers under dynamic density conditions that vary from a density of 1.0 x 1.5 meters to a density of 3.5 x 2.5 meters. the steps of: controlling the phenological cycle of the plant by illumination with intermittent wavelength pulses operating periodically over time and within the plant location space using an LED light system; artificially pollinating the flowers by applying pollen to the panicles of each flower using a spray system; controlling the size of the tree by training and pruning to form a multi-axis plant structure; controlling blight and disease by biological control using microorganisms; and cultivating large inner diameter fruits with small seed size.

The invention provides a hyperspectrum-based kiwi fruit disease identification method, device and equipment and a medium. The hyperspectrum-based kiwi fruit disease identification method comprises the following steps: acquiring and correcting a hyperspectral image of a to-be-detected kiwi fruit sample; performing region-of-interest segmentation and spectral data extraction on the corrected hyperspectral image; preprocessing the extracted spectral data; screening characteristic wavelengths of the preprocessed spectral data through a genetic algorithm; and completing kiwi fruit disease identification on the screened characteristic wavelengths through a constructed disease identification model. The method has the advantages of simplicity, wide range, no damage and the like, the occurrence degree of diseases can be accurately found as early as possible, corresponding prevention and control measures can be taken, the loss of fruit farmers is effectively reduced, and economic benefits are increased.

The present invention discloses Streptomyces SC-3 and its composition and use. The Streptomyces SC-3 strain is deposited in China Center for Type Culture Collection with the deposit number CCTCC NO:M 2024906. The present invention finds that the Streptomyces SC-3 strain itself has high efficiency and broad-spectrum antibacterial activity, and finds that the Streptomyces SC-3 strain has a significant antagonistic effect on kiwifruit soft rot bacteria. It is verified by indoor experiments that the speed of kiwifruit fruit decay caused by Phomopsis can be slowed down. Its fermentation liquid has good temperature stability, and a variety of antibacterial substances are identified from its sterile fermentation liquid, including antibiotics such as antimycin, tetramycin, candidacidin, streptomycin, etc., which are widely used in biological control. The speed of fruit decay caused by the infection of Botrytis cinerea after inoculation can be effectively inhibited.

The present disclosure refers to a method of intensive and sustainable cultivation of fruit trees with high water consumption, such as avocado, in confinement via multivariable management of: plant architecture, nutrition, dynamic densities, artificial pollination, and use of specific wavelengths for each phenological state that allow early entry into production, maximization of yield per unit area, and control of the time of fruit harvest. The method of growing fruit trees with high water consumption (for example, avocado, citrus, apple, mango, pear, peach, kiwi, among others), in containers for intensive fruit production, includes the stages of: having plants avocado with high root density in aerated containers; arrange in containers in a substrate for accelerated root growth; fertigating the containers with a nutrient solution that provides precocity in the vegetative development, flowering and fruiting of the plant; arranging the containers in conditions of dynamic density, which varies from a density of 1.0×1.5 meters to a density of 3.5×2.5 meters; controlling the phenological cycle of the plant by lighting with a system of LED lights with intermittent pulses of wavelengths that act cyclically over time and within the location space of the plants; artificially pollinating the flowers by applying pollen by a spray system to each floral panicle; controlling the size of the tree by driving and pruning to form a multi-axis type plant architecture; controlling pests and diseases through biological control with microorganisms; and cultivating the large-caliber fruit with a small seed size.

The invention provides a composition with a soothing effect, a preparation scheme of the composition and application of cosmetics. The composition comprises the following components in percentage by mass: 35-50% of actinidia chinensis fruit water, 2-10% of a radix gentianae extract, 1-9% of a citron fruit extract, 1-4% of a dendrobium stem extract, 1-5% of an oat extract and the balance of butanediol. All the components in the composition cooperate with one another, the multi-dimensional synergistic effect of resisting inflammation, resisting histamine, repairing a barrier, resisting oxidation and adjusting micro-ecology can be achieved, and the composition has the excellent relieving effect.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker for early identification of the cold resistance of an actinidia chinensis tree body in the dormancy period, and the SNP molecular marker is located at the Chr69297479 position on the No.6 chromosome of the actinidia chinensis and is closely associated with the cold-resistant phenotype median lethal temperature (LT50) of the actinidia chinensis. The method can be used for identifying the tree body dormancy period cold resistance of natural population and hybrid filial generation of actinidia chinensis. The homozygous genotype (CC) of the SNP locus corresponds to a high-cold-resistance individual in the dormancy period of an actinidia chinensis tree body, and the heterozygous genotype (TC) of the SNP locus corresponds to a low-cold-resistance individual. The cold resistance prediction accuracy in the F1-generation hybrid population reaches 94.94%, and the prediction accuracy in the natural population reaches 96.15%. The molecular marker detection method disclosed by the invention is convenient and rapid, has good specificity and high accuracy, is not influenced by climate, environment and the like, can rapidly and effectively evaluate the cold resistance of natural resources and hybrid filial generations of the actinidia chinensis, and provides powerful technical support for breeding cold-resistant varieties of the actinidia chinensis.

The invention discloses application of a natural inducer in fruit preservation, and particularly relates to the technical field of fruit preservation. According to the application of the natural inducer in fruit preservation, the natural inducer is used as an active ingredient to inhibit growth of botrytis cinerea on fruits and induce immune systems of the fruits to enhance active oxygen metabolism; the natural inducer is theanine; the fruits are kiwi fruits. When the natural inducer provided by the invention is applied to fruit preservation, a relatively good preservation effect can be exerted on kiwi fruits.

The invention belongs to the technical field of fruit tree planting, discloses a kiwi fruit multi-span greenhouse integrating rain sheltering and traction functions, and is mainly used for solving the problem that an existing kiwi fruit rain sheltering greenhouse cannot be used for traction of updated branches and tendrils of kiwi fruits. A rain sheltering shed frame structure is formed by the stand columns, the longitudinal beams, the upper cross beams, the main arch bars, the purlines and the shed film, meanwhile, the lower cross beams connected to the lower portions of the stand columns are connected with the multiple low-position bearing cables, the low-position bearing cables are used for laying and hanging fruiting mother branches on the low-position horizontal plane, fruits are exposed more easily, mechanical picking is facilitated, and the fruit picking efficiency is improved. In addition, a high-position inhaul cable is arranged above the low-position bearing cable, the high-position inhaul cable is connected with a pulling rope and is connected to the low-position bearing cable below in an inclined pulling mode, so that an inclined upward vine climbing route is formed, and new branches can grow upwards through the pulling rope, so that the new branches and fruiting mother branches below grow in a layered mode and do not interfere with each other. The layering of the vines can enhance the ventilation and lighting effects, improve the orchard environment and facilitate the improvement of the kiwi fruit quality.

The invention provides a gene AcHAIR for regulating and controlling development of epidermal hairs of kiwi fruits, the nucleotide sequence of the gene AcHAIR is shown as SEQ. ID. NO.1, and the coded amino acid sequence is shown as SEQ. ID. NO.2. The gene AcHAIR can be used for regulating and controlling development of epidermal hairs of kiwi fruits. The invention also provides a recombinant expression vector containing the AcHAIR gene, and an agrobacterium strain containing the AcHAIR gene. In addition, the invention also provides an application of the AcHAIR gene in cultivation of high-quality kiwi fruits. Overexpression of the AcHAIR gene can obviously promote growth of epidermal hair on leaves and stems in plants, and the AcHAIR gene is of great significance in exploring regulation and control mechanisms of development of the epidermal hair of kiwi fruits and cultivating high-quality kiwi fruit varieties.

The invention discloses lactococcus lactis M6 and application thereof, and belongs to the technical field of microorganisms. The lactococcus lactis M6 provided by the invention is obtained by separating and purifying a kiwi fruit sample picked in Sichuan of China, has multiple functions of high yield of gamma-aminobutyric acid (GABA), high yield of butyric acid, oxidation resistance, antibacterial performance and the like, and has great industrial application value.

Methods of farming, domesticating, or cultivating truffle fungi, including isolation and cultivation methods for producing pure truffle fungi, or mini-truffles, typically a Tuber spp. or an Iamai spp. Examples of truffle include Tuber melanosporum, Tuber magnatum, Tuber aestivum, Tuber uncinatum, Tuber borchii, Imaia spp., Tuber macrosporum, Tuber gibbosum, Tuber oregonense, and Tuber lyonii. The isolated pure truffle may then be cultivated on a nutrient substrate, which may be, for example, a fruit, nut, grain, or a portion thereof, resulting in a truffle-flavored food product. Non-limiting examples of such substrates include rye, barley, lentil, wheat, rice, soybeans, pecan, hazelnut, pine nut, English walnut, coffee beans, mustard, cacao, sesame, sunflower, grapes, blackberries, blueberries, cherries, kiwi, mango, raspberries, and huckleberries.

The invention provides a probiotic composition for inhibiting helicobacter pylori as well as a probiotic agent and application thereof, and belongs to the technical field of probiotic functional products. The probiotic composition is prepared from the following components in parts by weight: 0.05 to 2 parts of a pediococcus pentosaceus strain A21358, 0.01 to 1.5 parts of a plant lactobacillus strain A21136, 0.05 to 1.5 parts of a lactobacillus rhamnosus strain A21149 and 0.03 to 1.2 parts of a lactobacillus reuteri strain A21325. The probiotic agent comprises the probiotic composition, resistant dextrin, xylooligosaccharide, erythritol, inulin, fructo-oligosaccharide, hericium erinaceus powder, fucoidin and at least one fruit powder selected from grape powder, passion fruit powder and kiwi fruit powder, wherein the specific weight parts of the resistant dextrin, the xylooligosaccharide, the erythritol, the inulin, the fructo-oligosaccharide, the hericium erinaceus powder, the fucoidin and the at least one fruit powder are selected from grape powder, passion fruit powder and kiwi fruit powder. The probiotic agent has the effect of inhibiting helicobacter pylori, and a new thought is provided for clinical related auxiliary treatment of helicobacter pylori infection.

The invention discloses burkholderia gladioli BG-GF5 as well as a composition and application thereof, the burkholderia gladioli BG-GF5 is preserved in the China Center for Type Culture Collection with the preservation number of CCTCC NO: M20242573, and the preservation date is November 25, 2024. The application of the BG-GF5 is mainly reflected in the inhibition effect of the BG-GF5 on pathogenic bacteria and the potential of the BG-GF5 in crop disease control. The strain or the fermentation product and the metabolite thereof can be prepared into a composition for inhibiting pathogenic bacteria and crop diseases caused by the pathogenic bacteria. Particularly, the application to crops such as bee sugar plums, chilies and kiwi fruits shows a wide application prospect in the field of agricultural biological control.

The invention belongs to the technical field of genetic engineering, and relates to application of a transcription factor AcWRKY20 in resisting kiwifruit bacterial canker. Through gene cloning, phylogenetic analysis, subcellular localization, transgenic function verification and other technologies, it is proved for the first time that the transcription factor AcWRKY20 has the molecular characteristic of remarkably enhancing the disease resistance of kiwi fruits. According to the invention, the AcWRKY20 gene is respectively silenced and overexpressed and then is inoculated with Psa, and phenotypic observation shows that the gene is a disease-resistant gene and is suitable for canker-resistant breeding of kiwi fruit.

The invention discloses a traditional Chinese medicine compound extracted plant beverage which comprises the following raw materials in parts by weight: 20-30 parts of a traditional Chinese medicine compound extracting solution, 40-60 parts of composite concentrated fruit juice, 2-4 parts of a preservative, 0.2-0.5 part of a stabilizer and the balance of water, totaling 500 parts, the solid content of the traditional Chinese medicine compound extracting solution is 25-35%, and the traditional Chinese medicine compound extracting solution is prepared by extracting, filtering and concentrating radix astragali, fructus lycii, rhizoma dioscoreae, poria cocos, fructus crataegi and flos chrysanthemi; the solid content of the composite concentrated fruit juice is 30-40%, the composite concentrated fruit juice is prepared by pulping, filtering and concentrating blueberries, lemons, kiwi fruits and grapefruits, and the composite concentrated fruit juice contains a natural antioxidant. In addition, the invention further discloses a preparation method of the traditional Chinese medicine compound extracted plant beverage, and the traditional Chinese medicine compound extracted plant beverage not only is good in taste, but also has a remarkable fatigue relieving function and has a wide market application prospect.

The present invention discloses the application of isoquinoline-3-carboxylic acid in preventing and controlling plant bacterial diseases. The isoquinoline-3-carboxylic acid provided by the present invention has an obvious inhibitory effect on plant bacterial diseases, and the plant bacterial diseases are Ralstonia solanacearum, Acidovorax avenae subsp. Citrulli, Pseudomonas syringae pv. actinidiae, Pectobacterium carotovorum subsp. carotovorum, Xanthomonas oryzae pv. oryzae, Xanthomonas oryzae pv. oryzicola, Alternaria brassicae, Xanthomonas citri subsp. citri, Pectobacterium carotovorum subsp. carotovorum, Xanthomonas fragariae, Pseudomonas syringae pv. lachrymans, Dickeya dadantii, Xanthomonas axonopodis pv. mangiferaeindicae. The isoquinoline-3-carboxylic acid of the present invention has protective and therapeutic effects on Acidovorax avenae subsp. Citrulli.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to an AcAAT1 gene and application thereof in regulation and control of formation of fruit ester aroma. The invention provides a kiwi fruit AcAAT1 gene related to ester aroma formation and a protein coded by the kiwi fruit AcAAT1 gene, and constructs a corresponding overexpression vector and a transient silence vector which are transferred into kiwi fruits. Results show that the content of ester aroma in kiwi fruits can be increased by overexpressing the AcAAT1 gene, and the content of ester aroma in kiwi fruits can be reduced by silencing the AcAAT1 gene. The actinidia chinensis AcAAT1 gene disclosed by the invention can be used for promoting the formation of ester aroma. The method disclosed by the invention has very important application value in the direction of accurately creating new germplasm of the kiwi fruit with strong fragrance through a genetic improvement mode.