Method for easily authenticating flooding tolerance of kiwi fruit seedlings

Abstract

The invention discloses a method for easily authenticating flooding tolerance of kiwi fruit seedlings. The method comprises the following steps: a plastic box is buried in soil of a rain sheltering greenhouse, the edge of a box body and surrounding soil are arranged on the same horizontal plane, and nutrition bowls planted with the kiwi fruit seedlings are placed in the plastic box randomly; water is irrigated in the plastic box to enable root systems of the kiwi fruit seedlings to be completely immersed in the water; the situations of top tips and leaves of the kiwi fruit seedlings in the flooding process are recorded, the flooding time or the number of wilt leaves is used as an index, and flooding tolerance authenticating evaluation is carried out on various test materials. According to the method, the flooding time or the number of the wilt leaves can replace an existing complex evaluation system, the needed time is short, the operation is convenient, the result is obtained by testing several indexes of the survival rate, chlorophyll, peroxidase activity and root activity so as to comprehensively evaluate the stress resistance verification, the result is consistent with the visual flooding time or the number of the wilt leaves, and therefore the method is stable and reliable in result, easy to operate, and suitable for rapidly screening flooding tolerance seedling kiwi fruit seedling plants in a mass mode.

Description

technical field [0001] The invention relates to a simple identification method for waterlogging tolerance of kiwifruit seedlings, which belongs to the field of agricultural technology. Background technique [0002] Kiwifruit is an emerging fruit with great characteristics in my country. The industry is developing rapidly, and the planting area is widely distributed, ranking first in the world, so it is called "the hometown of kiwifruit". Although my country is the center of origin of Actinidia plants, the industry started relatively late. In the current germplasm resource identification method and system, the identification standards for three major traits of Actinidia botany, biology, and fruit have been determined, while waterlogging and stress resistance On the one hand, there is no mature technology and general identification methods and means, which brings blind spots to scientific research and production. In the process of breeding fine varieties, it is necessary to co...

AI Technical Summary

Problems solved by technology

The intuitive method is to control soil moisture through natural precipitation or irrigation, and record the impact of crop morphology and yield. This method is simple and subject to seasonal restrictions, too long time, heavy workload, slow speed, poor comparability, and difficult to repeat.

The indirect method is to simulate the plant environment through artificial water control, anti-waterlogging ponds, growth chambers, etc., to study the effects of plant growth and development, physiological processes, and biomass in different growth periods, so as to further evaluate the waterlogging resistance of varieties. The identification results of this method are compared. Reliable, easy to compare and repeat, but requires certain equipment, heavy workload, high cost, cannot be carried out in large quantities, and at the same time, differences with the actual field environment may cause errors

[0003] It is particularly necessary to establish a general, simple and rapid waterlogging tolerance identification method for kiwifruit seedlings. The invention patent of an evaluation and identification method for chrysanthemum waterlogging tolerance (application number CN200810124756.1) discloses a waterlogging tolerance identification method. Potted flooding identification method evaluates the test plants by selecting four indicators: leaf shape, leaf color, and stem color and shape at the flooded place. However, the evaluation system of this identification method is complicated and cumbersome, and it takes a long time (6 days), and it is different from the natural environment. Larger, and it is not conducive to rapid screening of waterlogging-tolerant plants in large quantities