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Kiwi fruit tissue culture method and application thereof

A tissue culture and kiwifruit technology, applied in the field of plant tissue culture, can solve the problems of tissue culture regeneration without Qionglu kiwifruit, and achieve the effects of improving induction rate, high survival rate and reducing pollution rate.

Inactive Publication Date: 2017-10-24
ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on kiwifruit tissue culture at home and abroad has been relatively mature, but so far there is no research on tissue culture regeneration of kiwifruit

Method used

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  • Kiwi fruit tissue culture method and application thereof
  • Kiwi fruit tissue culture method and application thereof
  • Kiwi fruit tissue culture method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] The selection of embodiment 1 explant

[0067] The invention adopts the newly germinated petioles, leaves and stems of Actinidia kiwifruit in spring as explant materials for disinfection and callus induction.

[0068] 1. Selection and sterilization of explants

[0069] Collect new shoots of "Qionglu" in the same year, use a small soft brush to scrub in detergent solution for 5-10 minutes, rinse with water for 3-4 hours, cut off the leaves and petioles, and cut the branches into 2-3cm stems segments (excluding axillary buds). Use 75% alcohol to sterilize leaves, petioles, and stems for 30 seconds in an ultra-clean workbench, rinse with sterile water once, then sterilize with 0.1% mercuric chloride for 40 seconds to 1 minute, and rinse with sterile water for 4 to 5 times. Dry the water with sterile filter paper, cut the leaves into 0.2 cm wide slender strips along the transverse veins, cut the petioles into 0.5 cm long, and cut the stems into 0.5-1 cm long for inoculati...

Embodiment 2

[0081] Comparison of different sterilization methods of embodiment 2

[0082] In order to overcome the incomplete sterilization of kiwifruit petiole by conventional disinfection methods, this application uses different methods to treat the petiole of Qionglu kiwifruit, and evaluates the pollution rate of petiole in the tissue culture process and the callus and variable number of petioles under different treatment methods. bud-inducing effect.

[0083] Treatment method 1: collect the young branches of "Qionglu" in the same year, use a small soft brush to scrub in the aqueous solution of detergent for 5-10 minutes, rinse with clean water for 3-4 hours, cut off the petiole, and use 75% water on the ultra-clean workbench Sterilize the petiole with alcohol for 30 seconds, rinse it once with sterile water, then sterilize it with 0.1% mercuric chloride for 2-3 minutes, and rinse it with sterile water 4-5 times. Dry the water with sterile filter paper, cut the petiole into 0.5 cm lon...

Embodiment 3

[0091] The optimization of embodiment 3 axillary bud germination medium

[0092] In order to improve the germination rate of axillary buds, the present application contains 30g·l -1 Sucrose and 7g·l -1 The MS medium of agar is used as the basic medium, and the types and contents of plant hormones are optimized in order to obtain the axillary bud germination medium with the best induction effect. The specific optimization method is as follows:

[0093] (1) Add the hormone combination prepared according to Table 3 into the basal medium to prepare different axillary bud germination medium; (2) Sterilize the axillary bud stem section of "Qionglu" kiwifruit and transfer them to each medium for Aseptic culture, disinfection mode and culture condition refer to embodiment 2 treatment mode 2; (3) record the germination situation of axillary bud.

[0094] Hormone combination formula of table 3 axillary bud germination medium

[0095]

[0096]

[0097] Table 4 Germination of ax...

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Abstract

The invention provides a kiwi fruit tissue culture method and an application thereof. According to the method, axillary buds are germinated under an aseptic condition to form a leaf stalk serving as an explant, formation of callus tissues is induced, and then the callus tissues are cultured into kiwi fruit seedlings. According to the culture method, the contamination rate of a tissue culture process can be reduced, and the induction rate, the germination rate and the rooting rate of the callus tissues are increased. In addition, the kiwi fruit seedlings cultured by the method have the advantages of strong growth, developed root systems and high transplantation survival rate.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a kiwi fruit tissue culture method and application thereof. Background technique [0002] Kiwi (Actinidia Chinensis) is a woody vine with high economic value. Its fruit is thick and juicy, moderately sweet and sour, and rich in nutrition. It has become a treasure among current fruits because of its high nutritional value of vitamin C. Emerging cultivated fruit trees in today's world. [0003] Qionglu kiwifruit is an excellent strain selected from the wild resources of Xixia, Henan Province in 1978 by the Zhengzhou Institute of Fruit Trees, Chinese Academy of Agricultural Sciences. The weight of a single fruit is 70-105 grams, the skin is yellowish brown, the flesh is light yellow, juicy, sweet and sour, slightly fragrant , the content of vitamin C is 241-318 mg / 100 grams of fresh pulp, and the soluble solids are 19%. Cooked varieties. [0004] With the rise of kiwifruit cul...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 刘丽
Owner ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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