Multiploid bulb induction method with firtillaria cirrhosa scale leaf as explant

Abstract

The invention discloses a multiploid bulb induction method with a firtillaria cirrhosa scale leaf as an explant. The innovation of the invention lies in that a used material is a close callus with a certain differentiation characteristic, which is obtained through a predifferentiation culture process on the basis of generating a callus through induction of the scale leaf and has. The material is cut into small blocks and is soaked into a colchicine solution to carry out multiploid mutagenesis treatment, thereby greatly lightening damage of the colchicine solution to a processing material and enhancing the inductivity of the firtillaria cirrhosa multiploid bulb.

Description

technical field

[0001] The invention relates to a method for inducing polyploid bulbs, in particular to a method for inducing polyploid bulbs using scale leaves of fritillary fritillaria as explants. Background technique

[0002] The medicinal materials of Fritillaria sichuan are the dried bulbs of F. cirrhosa, F. unibracteata, F. przew alskii Maxim. and F. delavayi Franch. of Liliaceae plants. One of the native medicinal materials, the bulb is used as medicine. Fritillaria fritillata plants have strict requirements on the growth environment. It likes cold and cloudy climate conditions, and has the characteristics of cold resistance, humidity, shade, and high temperature. When the temperature reaches 30°C or the ground temperature exceeds 25°C, the plants will wither and cannot survive in areas with low altitude and high temperature. Therefore, fritillary fritillaria can grow normally at an altitude of 3500m-4500m. Due to the limitation of growth conditions and unplanned a...

Examples

Embodiment 1

[0017] (1) Obtain the compact callus of Fritillaria volata

[0018] a. Select the fresh bulbs of Fritillaria convolata planted in Zheduoshan wild tending base in Kangding, Sichuan, peel off the scale leaves with no disease and no mechanical damage in the inner layer, wash them with washing powder, rinse them under running water for 30 minutes, and then use Rinse with 2% sodium hypochlorite solution for 15 minutes, wash with clean water and blot dry, put the treated scale leaves in 0.1% mercuric solution on the ultra-clean workbench for 9 minutes, rinse with sterile water for 3 times, and then use sterile Blot the surface water with filter paper, and finally insert it into MS+6-BA1mg·L -1 +IAA0.5mg·L -1 + sucrose 30g·L -1 +Agar 6.5g·L -1 Under the culture conditions of temperature 18-22°C, 8-16 hours of light per day, and light intensity of 1200lx, the induction culture was carried out in the culture medium of 18-22°C, and the dark culture was carried out for 14 days at the ...

technical field

[0001] The invention relates to a method for inducing polyploid bulbs, in particular to a method for inducing polyploid bulbs using scale leaves of fritillary fritillaria as explants. Background technique

[0002] The medicinal materials of Fritillaria sichuan are the dried bulbs of F. cirrhosa, F. unibracteata, F. przew alskii Maxim. and F. delavayi Franch. of Liliaceae plants. One of the native medicinal materials, the bulb is used as medicine. Fritillaria fritillata plants have strict requirements on the growth environment. It likes cold and cloudy climate conditions, and has the characteristics of cold resistance, humidity, shade, and high temperature. When the temperature reaches 30°C or the ground temperature exceeds 25°C, the plants will wither and cannot survive in areas with low altitude and high temperature. Therefore, fritillary fritillaria can grow normally at an altitude of 3500m-4500m. Due to the limitation of growth conditions and unplanned a...

Examples

Embodiment 1

[0017] (1) Obtain the compact callus of Fritillaria volata

[0018] a. Select the fresh bulbs of Fritillaria convolata planted in Zheduoshan wild tending base in Kangding, Sichuan, peel off the scale leaves with no disease and no mechanical damage in the inner layer, wash them with washing powder, rinse them under running water for 30 minutes, and then use Rinse with 2% sodium hypochlorite solution for 15 minutes, wash with clean water and blot dry, put the treated scale leaves in 0.1% mercuric solution on the ultra-clean workbench for 9 minutes, rinse with sterile water for 3 times, and then use sterile Blot the surface water with filter paper, and finally insert it into MS+6-BA1mg·L -1 +IAA0.5mg·L -1 + sucrose 30g·L -1 +Agar 6.5g·L -1 Under the culture conditions of temperature 18-22°C, 8-16 hours of light per day, and light intensity of 1200lx, the induction culture was carried out in the culture medium of 18-22°C, and the dark culture was carried out for 14 days at the ...