21 results about "Stratification (seeds)" patented technology

The invention discloses a real-time detection method for bulbus fritillariae propagating activity, and relates to the field of seedling detection, and the method comprises the following steps: step 1, seed stratification pretreatment: mixing bulbus fritillariae seeds and fine sand according to a ratio of 1: 3 for stratification, and maintaining the humidity of a substrate to be 50-60%; 2, dynamically monitoring enzyme activity, namely grinding the sample prepared in the step 1, sampling and detecting the seeds every 10 days, extracting enzyme liquid, and respectively measuring the activity of catalase, peroxidase and amylase in real time by adopting an ultraviolet spectrophotometric method; a full-cycle real-time data flow is constructed, a continuous dynamic process of activity change is captured, then a machine learning model is used for fusion analysis and prediction of seed propagation potential, activity evaluation is converted into a quantifiable propagation activity index, a clear judgment threshold value is set, and the machine learning model is trained on the basis of a large amount of data, so that the propagation activity of seeds is improved. And the accuracy and efficiency of high-quality seed source screening are improved.

The invention belongs to the technical field of agricultural breeding, and discloses a method for improving the germination rate of glehnia littoralis seeds, which comprises the following steps: (1) seed pretreatment: screening the glehnia littoralis seeds harvested in the current year, removing impurities, and removing malformed, worm-damaged and empty seeds; (2) pre-absorbing water by the seeds: soaking the screened seeds in clear water, and draining water on the surfaces of the seeds after the seeds are fully absorbed and swollen; (3) hormone solution preparation and seed soaking: preparing an indolebutyric acid solution, soaking the cleaned seeds in the indolebutyric acid solution, and performing heat preservation and seed soaking; and (4) low-temperature lamination: mixing the soaked seeds with sterile wet sand, and laminating in a low-temperature environment. According to the method, the germination rate and germination uniformity of the glehnia littoralis seeds can be remarkably improved, and the requirement for large-scale planting of the glehnia littoralis

The invention relates to the technical field of plant tissue culture and endangered tree seed breeding, in particular to an efficient embryo germination culture method for breaking through the deep dormancy characteristic of tilia amurensis, and aims at solving the problems that existing tilia amurensis seed breeding is low in germination rate, long in period, high in pollution rate and poor in embryo seedling quality. The method comprises the following steps: selecting robust tilia amurensis seed trees in specific areas such as 129 degrees, 19 '+ / -0.5 degrees and the like of the east longitude, and harvesting seeds with the water content of 45.25%-59.06% from August 29 to September 20; stratification is conducted at the low temperature of 4 DEG C for 20 days, 50 mg / L GA3 is added, soaking is conducted for 12 h, and pretreatment Precisely disinfecting with 75% alcohol and 2% sodium hypochlorite; cutting a 1-2mm notch on the seed coat, and then carrying out antioxidant treatment by using a vitamin C-humic acid solution; inoculating to an optimized MS culture medium containing hormones such as 0.1 mg / L 6-BA, carrying out dark culture at 22 DEG C for 5 days, and then carrying out 2000lx illumination culture at 24 DEG C; according to the method, deep dormancy is efficiently broken, the pollution rate is low, embryo seedlings are robust, and large-scale breeding and population recovery of tilia amurensis can be supported.

The invention discloses a malania oleifera seed germination accelerating method and application thereof, and the method comprises the following steps: (1) applying mechanical force to malania oleifera seeds, so as to generate cracks on seed shells of malania oleifera to conduct the inner space of the seed shells and the outer part of the seed shells; the crack longitudinally extends on the seed shell and penetrates through the top of the seed shell; (2) soaking the seeds treated in the step (1) in an aqueous solution containing a dormancy breaking agent; the dormancy relieving agent comprises gibberellin and a cytokinin analogue; and (3) sowing the seeds treated in the step (2) in a breathable and water-containing culture medium to perform stratification and germination acceleration, and performing temperature control and regular irrigation in the period until the seeds germinate. According to the application, the method is applied to germination acceleration of malania oleifera seeds collected from wild malania oleifera plants in Guangxi Zhuang Autonomous Region and / or Yunnan.

The invention discloses a method for increasing the sowing survival rate of fritillaria taipaiensis seeds, and belongs to the technical field of fritillaria taipaiensis breeding. Fritillaria taipaiensis seeds are embedded with an embedding material and then subjected to variable-temperature stratification treatment, all components in the embedding material act together, and the survival rate of the fritillaria taipaiensis seeds is increased; the morphological after-ripening and physiological after-ripening of the seeds are sequentially promoted by combining temperature-variable stratification treatment, so that the fritillaria taipaiensis seeds can better reach a germination state, the seed vitality is improved, the sowing survival rate of the fritillaria taipaiensis seeds is improved, and the fritillaria taipaiensis seed sowing method has important significance in promoting the industrial development of fritillaria taipaiensis.

This invention relates to the field of agricultural biotechnology and discloses a ternary hormone complex solution for promoting the germination of tetraploid Polygonatum sibiricum seeds. By mass percentage, it consists of the following raw materials: gibberellin 0.005%–0.02%, 6-benzyladenine 0.0005%–0.002%, salicylic acid 0.0005%–0.002%, with the balance being water. The germination method includes the following steps: S1: seed pretreatment; S2: hormone soaking; S3: substrate sterilization; S4: sand stratification for germination. This ternary hormone complex solution and germination method can significantly improve the germination rate of tetraploid Polygonatum sibiricum seeds and effectively reduce the mold rate during the seed germination period.

The invention discloses a root promoting nutrient solution and a herba epimedii root strengthening and bud increasing seedling raising method, and the method comprises the following steps: performing seed initiation on herba epimedii seeds, performing sand storage and stratification for 60-90 days, and accelerating germination until white buds appear; sowing the white and sprouted seeds on the seedbed, covering the seeds with a substrate, and growing seedlings; when 1-2 true leaves of each seed seedling expand and stem nodes extend to 3-5 cm, terminal buds are completely covered with humic fine soil with the pH being 5.5-6.5, and the soil covering thickness is 1.0-2.0 cm; 2-4 L of a root-promoting nutrient solution is used in each square meter of the seedbed after the seedbed is covered with the soil, and the root-promoting nutrient solution is prepared by adding 150-200 mg of algal polysaccharides, 1.1-1.66 mg of salicylic acid and 800-1200 mg of monopotassium phosphate into each 1 L of water; 30-60 days after the seedbed is covered with soil, when axillary seedlings exist, after more than or equal to 5 secondary roots are formed at the base parts of the axillary seedlings, stem nodes connecting the mother seedlings and the axillary seedlings are transversely cut off in the middle, and the distance between notches and the base parts of new seedlings is 2 + / -0.5 cm; a wound healing nutrient solution is sprayed to notches of the mother seedlings and the new seedlings, and the mother seedlings and the new seedlings are cultured to adult seedlings respectively. The problems that an existing epimedium breeding method is low in breeding coefficient, low in seedling quality and the like are solved.

The present invention belongs to the field of plant seedling cultivation, and specifically relates to a method for sowing and raising seedlings of Hamamelis chinensis, which specifically includes: collecting seeds from mid-September to early October; removing impurities from the seeds and drying them in the shade until the moisture content of the seeds is 8% to 10%; storing the seeds in sand over the winter by dynamically controlling the humidity and adopting the "sand-seed-sand" stratification method, and turning and disinfecting the seeds multiple times during the sand storage period; sowing the seeds when the germination rate reaches 20% to 30% in the following year, and covering them with fine soil and hay; gradually removing the hay during the period when the cotyledons of 20% to 30% emerge and unfold, and carrying out conventional seedling management. The emergence rate of Hamamelis chinensis cultivated using the method provided by the present invention can reach 80% to 85%, and the seedling survival rate can reach 95% to 98%. ， The seedlings are robust and have strong adaptability to the environment. At the same time, the method is simple to operate, easy to master and has low cost, and has important practical significance for the resource protection, scientific research and development and utilization of the Hamamelis species.

The invention discloses an epimedium seed stratification treatment method, and belongs to the technical field of traditional Chinese medicinal material cultivation, and the method comprises the following steps: S1, seed initial physiological status evaluation: collecting Wushan epimedium seeds of the same batch, selecting a part of seed samples for respiration intensity determination, placing the part of seeds in a closed respiration chamber, and carrying out primary physiological status evaluation on the Wushan epimedium seeds; a high-precision gas analyzer is used for continuously monitoring the change of gas components in the breathing chamber, and the release amount of CO2 and the consumption amount of O2 in unit time are calculated; and after the respiration intensity measurement is completed, continuously using the hyperspectral imaging technology to obtain spectral reflectivity images of the part of seed samples, obtaining spectral reflectivity images of the seeds under different wavelengths, processing the spectral reflectivity images under different wavelengths, and extracting the spectral reflectivity under the alpha-amylase absorption wavelength. The seed deposition treatment effect can be improved, and the stability and consistency of the seed deposition treatment effect of each batch can be improved according to the physiological state.

The invention relates to a posture-adjusting and filling-promoting centrifugal corn high-speed precision seed-metering device and a seed-metering method, and belongs to the technical field of harvesting equipment in agricultural machinery, the posture-adjusting and filling-promoting centrifugal corn high-speed precision seed-metering device comprises a rear shell, a bearing, a seed-metering disc, a flow guide turbine, a transmission shaft, a seed protection plate, a hole insert, a layered annular partition plate and a front shell; the seed metering disc comprises a posture adjusting groove; the layered annular partition plate comprises sieve holes. According to the seed-metering device, single-grain ordered seed metering of corn seeds is achieved through the centrifugal effect, population layering and posture adjustment are achieved through the synergistic effect of a flow guide turbine and a layered annular partition plate, seeds are filled in the thickness direction by combining the cooperation of a posture adjusting groove and screen holes, and the problems that due to the fact that the shapes of the corn seeds are irregular, inter-seed resistance is large, seed filling time is short, and seed filling efficiency is high are solved. And invalid seed filling such as seed blocking and blockage of the seed metering device in high-speed operation is easy to occur. Compared with a pneumatic high-speed seed-metering device, the structure of the seed-metering device is simplified under the condition of ensuring high-speed precise seed metering, and meanwhile, the operation energy consumption is greatly reduced.

The invention discloses a boschniakia rossica symbiotic cultivation method with alnus japonica as hosts, and belongs to the technical field of boschniakia rossica cultivation The problem that boschniakia rossica symbiotic cultivation is low in survival rate and unstable in yield is solved. The method comprises the following steps: selecting a healthy alnus japonica middle-age forest with the tree age of 10-30 years as a host forest; oblique cuts are cut in the lateral roots of the alnus japonica at the positions 20-40 cm away from the trunk of the alnus japonica; mixing the boschniakia rossica seeds subjected to cold stratification treatment and hormone combined treatment with a biological adhesive to prepare seed paste; coating a root nodule symbiotic nitrogen-fixing actinomycete inoculant at a host root notch, filling the seed paste into the host root notch, covering the host root notch with wet humus soil, and compacting; the humidity of the covered wet humus soil is kept to be 15%-25%, low-concentration compound fertilizer and microbial agents are dressed in May, inoculation points are checked in June and September every year, and inoculation is supplemented if the parasitic rate is lower than 80%; after the boschniakia rossica germinates in the spring of the second year, collecting flower stems.

The invention relates to the technical field of plant breeding, and discloses a breeding method of endangered angiosperm saruma henryi, which comprises the following steps: sexual propagation treatment: treating saruma henryi seeds to obtain saruma henryi seedling groups, the treatment comprising soaking the saruma henryi seeds for 36-60 hours by using a gibberellin solution with the concentration of 150-250mg / L; single plant screening: screening excellent single plants with preset excellent characters from the saruma henryi seedling population; and cutting propagation treatment: treating the excellent single plant to realize amplification. By adopting the sexual propagation treatment step of combining stratification treatment and gibberellin solution soaking in a specific parameter range, the deep dormancy problem of the saruma henryi seeds is specifically solved, the dormancy state of the saruma henryi seeds can be effectively relieved, and therefore the high seed germination rate is obtained.

The invention relates to an efficient comprehensive propagation method suitable for carpinus zenii, and belongs to the technical field of forest tree breeding. The method is characterized in that two propagation systems of asexual cutting and seed seedling raising are integrated in the method, the asexual cutting propagation system is characterized by mainly comprising the steps of cutting preparation, matrix preparation and cutting, rooting, transplanting management and the like, and the cutting is from carpinus zenii seedlings which are 3-5 years old and have high C / N leaves; the seed seedling breeding system is characterized in that the seed seedling breeding system mainly comprises the steps of seed collection and screening, variable temperature stratification treatment, sowing and germination management, seedling maintenance and the like, and the variable temperature stratification treatment refers to the process that sand-stored seeds are placed under the natural conditions of shading and ventilation for overwintering so as to complete variable temperature stratification. According to the comprehensive complete set of technical scheme, nursery stocks with genetic consistency and genetic variation can be obtained, and artificial populations with rich genetic diversity can be cultivated. The C / N of seedling leaves is increased through sufficient illumination and fertilizer control, cutting slips are collected in the morning and the like, a large number of cutting slips successfully take roots and grow seedlings, and the seedling rate of the cutting slips reaches 73.11%; the seedling rate of the full seeds can reach 83.95% through measures of variable-temperature stratification of the sand-stored seeds under natural conditions, delayed seedling transplanting and the like.

The invention provides a bock greenbrier rhizome seed seedling raising method, and belongs to the technical field of seed seedling raising. The method comprises the following steps: disinfecting bock greenbrier rhizome seeds, placing the disinfected bock greenbrier rhizome seeds and wet sand in a layered manner, carrying out stratification germination acceleration for 5-7 months, laying a seed layer with the thickness of 0.3-0.8 cm on each wet sand layer with the thickness of 0.5-1.5 cm, then sowing the germinated seeds, carrying out seedling culture to obtain seedlings, and transplanting the seedlings to a field. According to the method, the germination rate of the seeds can be increased, the obtained seedlings are high in vigor and transplanting survival rate, and the seedling raising time of the bock greenbrier rhizome seeds can be greatly shortened from 3-4 years of a traditional seedling raising method to 1 and a half years. The technical details involved in the method are simple to operate and easy to master, and the ecological environment cannot be greatly influenced.

The invention discloses a switchgrass sowing propagation method, and belongs to the technical field of switchgrass propagation, a composite embedding agent is prepared to embed switchgrass seeds, then low-temperature sand storage stratification treatment is carried out, fluridone is dynamically released through the composite embedding agent, low-temperature treatment is combined to promote switchgrass seed dormancy breaking, and the switchgrass seed dormancy breaking rate is increased. The composite embedding agent can continuously provide an appropriate humidity environment for the seeds after the stratification treatment is completed, so that the secondary dormancy of the seeds is inhibited, the germination rate of the seeds is improved, the sowing and propagation efficiency of switchgrass is improved, and the method has a good application prospect in the field of switchgrass propagation.

The invention discloses a seed warehouse stratified sampling device, and relates to the field of grain seed storage, the seed warehouse stratified sampling device comprises a sampling pipe, the sampling pipe adopts a hollow tubular structure, a plurality of sampling ports are equidistantly formed in the surface of the sampling pipe along the length direction, and a partition plate is arranged above each sampling port; the interior of the sampling pipe is divided into a plurality of independent sampling partition cavities at equal intervals through partition plates, a main shaft is installed in the center of the interior of the sampling pipe in the axial direction in a penetrating mode, and a sealing assembly used for opening a sampling opening and an auxiliary sampling assembly used for auxiliary sampling are installed at the position, located at each sampling opening, of the main shaft. The multiple independent sampling separation cavities distributed in the length direction of the sampling pipe at equal intervals are matched with the sealing assemblies and the auxiliary sampling assemblies which can be synchronously opened and closed at the sampling openings, so that the layered sampling accuracy is effectively ensured, mutual doping of seeds of different depths can be thoroughly avoided, and the specific state of the seeds of each layer is truly reflected.

This invention belongs to the field of ecological environment restoration technology and discloses a method for ecological restoration of grassland vegetation in photovoltaic power station areas in Gobi deserts. The method uses planted semi-shrubs as the core framework, employing micro-topography modification and seedling technology to effectively stabilize and improve gravelly soil, enhancing the stability of the topsoil structure. A specific ratio of mixed grass seeds is sown within a 15-30cm radius around the planted plants, forming a semi-shrub-herbaceous multi-layered structure. The complementary functions and vertical stratification of these two types of grasses promote stable community succession. Furthermore, the application of a specific ratio of organic fertilizer and water-retaining agent improves the soil at the bottom of the planting pit. This method not only avoids the waste associated with large-scale seed sowing but also significantly improves seedling emergence and survival rates, rapidly increasing vegetation coverage, reducing maintenance frequency and costs, and has broad application prospects in the ecological restoration of gravelly land.

The present application relates to the technical field of plant breeding, and discloses a breeding method of endangered angiosperm Alipelta, comprising the following steps: sexual reproduction treatment: treating Alipelta seeds to obtain a group of Alipelta seedlings, wherein the treatment comprises soaking the Alipelta seeds in a gibberellin solution with a concentration of 150-250 mg / L for 36-60 hours; single plant screening: screening an excellent single plant with preset excellent traits from the group of Alipelta seedlings; cutting propagation treatment: treating the excellent single plant to realize amplification. Through the sexual reproduction treatment step of combining stratification treatment with soaking in a gibberellin solution with a specific parameter range, the deep dormancy problem of the Alipelta seeds is solved, and the dormancy state of the Alipelta seeds is effectively released, so that a high seed germination rate is obtained.

This invention relates to the field of Chinese medicinal herb cultivation technology and discloses a method for large-scale seed propagation of Polygonatum multiflorum. The method includes the following steps: (1) selecting mother plants to harvest fruits, softening them by composting, and then removing the pulp by mechanical rubbing to obtain clean seeds; (2) subjecting the clean seeds to low-temperature stratification treatment at 0-5℃ for 20 days, and then soaking them in a plant growth regulator obtained by diluting 4wt% gibberellin 800 times for 12-24 hours; (3) placing the soaked seeds in moist sand in a wooden box in an arched shed for sand stratification and germination at 1-10℃; (4) after the seed whitening rate reaches 15%, sowing them in a seedling substrate and carrying out seedling management. This invention effectively overcomes the technical bottlenecks of low germination rate, uneven emergence, and frequent diseases in traditional methods, and realizes the overall optimization of seedling quality and standardized control of the production process, and has good prospects for industrialization and promotion.

A method for breaking dormancy of Thesium chinense Turcz. seeds, which includes: immersing collected seeds of Thesium chinense Turcz. in water, selecting seeds that sink in the water followed by air-drying, and storing at 2-4° C. to obtain to-be-used seeds; immersing the to-be-used seeds in a gibberellin solution, taking out the seeds and mixing with river sand under −4-2° C. for a first stratification treatment; rinsing off the river sand, removing green skins and stalks of the seeds after the first stratification treatment, keeping the seeds moist and placing the seeds under 2-4° C. for a second stratification treatment to obtain ruptured seeds; and using the ruptured seeds in a three-slit stage as a germination material, and culturing the ruptured seeds in a three-slit stage in a dark environment at 17-22° C. for seed germination. The method can significantly improve the germination rate of Thesium chinense Turcz. seeds.

The invention discloses a safe idesia seed germination induction method. The method comprises the following steps: (1) removing wax on the surfaces of idesia seeds, washing and draining to obtain wax-removed idesia seeds; (2) putting the idesia seeds subjected to wax removal into a gibberellin GA3 solution with the concentration of 0-200mg / L, shaking and soaking for 24-36 hours, washing and draining to obtain the idesia seeds treated by GA3; (3) after sterilizing and disinfecting the idesia seeds, transferring the idesia seeds into a metaphosphoric acid aqueous solution with the mass concentration of 10-50%, shaking and soaking for 25-45 minutes, carrying out germination promoting treatment, washing and draining to obtain the idesia seeds subjected to germination promoting treatment; and (4) spreading the idesia polycarpa seeds subjected to germination promoting treatment into a germination induction culture medium for germination induction. According to the method, a controlled dangerous chemical reagent is not used, new or old seeds are not picked out, the seeds do not need to be laminated, the seeds can germinate in 5 days and enter a germination peak in 15 days, the germination rate can reach 92.59%, and the method is an extremely efficient and safe idesia polycarpa seed germination induction method.