Root promoting nutrient solution and epimedium root strengthening and bud increasing seedling raising method

Abstract

The invention discloses a root promoting nutrient solution and a herba epimedii root strengthening and bud increasing seedling raising method, and the method comprises the following steps: performing seed initiation on herba epimedii seeds, performing sand storage and stratification for 60-90 days, and accelerating germination until white buds appear; sowing the white and sprouted seeds on the seedbed, covering the seeds with a substrate, and growing seedlings; when 1-2 true leaves of each seed seedling expand and stem nodes extend to 3-5 cm, terminal buds are completely covered with humic fine soil with the pH being 5.5-6.5, and the soil covering thickness is 1.0-2.0 cm; 2-4 L of a root-promoting nutrient solution is used in each square meter of the seedbed after the seedbed is covered with the soil, and the root-promoting nutrient solution is prepared by adding 150-200 mg of algal polysaccharides, 1.1-1.66 mg of salicylic acid and 800-1200 mg of monopotassium phosphate into each 1 L of water; 30-60 days after the seedbed is covered with soil, when axillary seedlings exist, after more than or equal to 5 secondary roots are formed at the base parts of the axillary seedlings, stem nodes connecting the mother seedlings and the axillary seedlings are transversely cut off in the middle, and the distance between notches and the base parts of new seedlings is 2 + / -0.5 cm; a wound healing nutrient solution is sprayed to notches of the mother seedlings and the new seedlings, and the mother seedlings and the new seedlings are cultured to adult seedlings respectively. The problems that an existing epimedium breeding method is low in breeding coefficient, low in seedling quality and the like are solved.

Description

A root-promoting nutrient solution and a method for promoting root growth and bud proliferation of Epimedium. Technical Field

[0001] This invention relates to the field of plant cultivation technology, and in particular to a root-promoting nutrient solution and a method for promoting root growth and bud proliferation of Epimedium. Background Technology

[0002] Epimedium plants are a precious traditional Chinese medicine resource, and their dried leaves are a core ingredient in many prepared Chinese medicines. With rapidly increasing market demand, wild Epimedium resources are nearing depletion due to over-harvesting, making large-scale artificial cultivation the fundamental way for the industry's sustainable development. However, significant bottlenecks exist in the artificial propagation of Epimedium, severely restricting its industrial development.

[0003] Currently, production mainly employs division propagation, tissue culture technology, and seed propagation. While division propagation can shorten the seedling cycle and maintain the superior traits of the mother plant, its propagation coefficient is extremely low (only 3-5 times per year), and it consumes a huge amount of perennial mother plant resources, making it difficult to provide a large number of seedlings in a short period of time. Furthermore, the quality of the seedlings varies, affecting yield.

[0004] While tissue culture technology offers a new approach to solving the problem of propagation efficiency, it is complex to operate, costly, relies on exogenous hormones, has strict requirements for facilities and professional technicians, and has an unstable transplant survival rate, making it difficult to promote among ordinary growers.

[0005] The seed propagation process, as shown in patent invention number CN201910327511.7, mainly involves germinating dormant seeds to achieve seedling cultivation. However, due to their deep dormancy, the natural germination rate of seeds is extremely low (usually below 30%), and the seedling cultivation cycle is long (more than 12 months). After one year of planting, the number of buds is also small (more than 3). Since this cannot meet the needs of rapid propagation, the survival rate of seedlings after transplanting is also low. Summary of the Invention

[0006] To address the shortcomings of existing technologies, this invention provides a root-promoting nutrient solution and a method for cultivating Epimedium with strong roots and increased buds, which solves the problems of low propagation coefficient, poor seedling quality, and low survival rate of seedlings after transplanting in existing Epimedium propagation methods.

[0007] To achieve the above objectives, the present invention provides a root-promoting nutrient solution with the following formulation: 150-200 mg of seaweed polysaccharide, 1.1-1.66 mg of salicylic acid, and 800-1200 mg of potassium dihydrogen phosphate are added to every 1 L of water.

[0008] Compared with existing technologies, this invention has the following beneficial effects: This invention creates a unique root-promoting nutrient solution containing seaweed polysaccharides and salicylic acid. This root-promoting nutrient solution, through a three-dimensional synergistic design of "polysaccharide water retention - salicylic acid signal regulation - phosphorus and potassium nutrient supply", breaks through the limitations of traditional single-component or simple compound nutrient solutions. It has a certain root-promoting effect on different species of Epimedium (Epimedium coarse hair, Epimedium sagittatum, Epimedium soft hair), shortening the rooting time of new Epimedium seedlings and increasing biomass. Especially after one year of transplanting, the increase in biomass is more obvious. At the same time, it can also improve the survival rate after transplanting. The three components in the root-promoting nutrient solution function synergistically, and the innovative mechanism provides a comprehensive root cultivation system for seedling cultivation. It provides an efficient, safe, and stable solution for modern agricultural precision seedling cultivation, and is especially suitable for large-scale production scenarios such as facility seedling cultivation and stress seedling cultivation.

[0009] Furthermore, the EC value of the root-promoting nutrient solution is 0.8-1.2 mS / cm.

[0010] On the other hand, the present invention also provides a method for promoting root growth and seedling development of Epimedium, comprising the following steps: (S1) Seed pretreatment: Epimedium seeds are subjected to seed priming, then stratified in sand for 60-90 days until germination occurs; (S2) Sowing and seedling raising: The germinating seeds from step (S1) are evenly sown on a seedbed, covered with substrate, and raised as seedlings; (S3) Soil covering and induction: When the seedlings from step (S2) have developed 1-2 true leaves and the stem nodes have elongated to 3-5 cm, the terminal buds are completely covered with fine humus soil with a pH of 5.5-6.5, with a soil covering thickness of 1.0-2.0 cm. (S4) Rooting induction: Irrigate the seedbed after covering with soil in step (S3) with 2-4L of the root-promoting nutrient solution described in claim 1 or 2 per square meter of seedbed; (S5) Separation of axillary seedlings: 30-60 days after covering with soil in step (S4), when axillary seedlings are present, after ≥5 secondary roots have formed at the base of the axillary seedlings, cut the stem node connecting the mother seedling and the axillary seedling in the middle horizontally, with the cut 2±0.5cm away from the base of the new seedling; (S6) Independent cultivation: Spray the cut of the mother seedling and the axillary seedling in step (S5) with wound healing nutrient solution, and cultivate the mother seedling and the axillary seedling separately until they become seedlings.

[0011] Compared with existing technologies, this invention has the following beneficial effects: 1. This invention innovatively proposes precise soil covering treatment (1.0-2.0cm thick) during the seedling leaf expansion stage (when 1-2 true leaves have unfolded). By eliminating apical dominance, it activates the axillary buds of the stem nodes to develop into independent new seedlings, promoting axillary bud development and increasing the potential for single-plant seedling growth. Furthermore, due to the soil covering treatment, the number of roots, buds, and root weight of the seedlings can be significantly increased, improving seedling quality, increasing the survival rate after transplanting, and also increasing the yield of Epimedium.

[0012] 2. Covering the seeds with soil can promote the formation of axillary roots, thus enabling one seed to produce two seedlings, with a double seedling probability of over 20%, which can meet the needs of rapid propagation.

[0013] 3. When separating axillary seedlings, after the axillary seedlings have formed more than 5 secondary roots, the stem nodes are cut horizontally (in the middle of the stem node) to make the two seedlings completely independent. Combined with wound healing nutrient solution treatment, it can realize the potential for multiple seedlings and realize the operation of large-scale seedling production in a short time.

[0014] 4. By using a newly designed root-promoting nutrient solution, the rooting time of new seedlings is shortened and the biomass is increased. Combined with precise soil covering during the leaf expansion period of Epimedium seedlings, the propagation efficiency is greatly improved compared with traditional methods. At the same time, the number of seedling roots, the number of buds and the root weight are significantly increased. Moreover, the operation is simple and the cost is low, making it suitable for large-scale industrial application and providing an innovative solution to the problem of Epimedium resource shortage.

[0015] Further, in step (S1): the seed initiation is as follows: soak the Epimedium seeds in 0.05%-0.15% KNO3 solution for 20-28 hours, then soak them in 500 times carbendazim solution for 1-1.5 hours, take them out, clean them, and then dry them.

[0016] Further, in step (S2), the seedbed treatment steps are as follows: Select a relatively flat, well-drained, irrigated, and easily managed sparse woodland or cultivated land as a nursery. The nursery is built on cultivated land with a shading net with a shading degree of 60%-70%. After the nursery is leveled, build a bed 1.2m wide and 0.2m high. After the bed surface is leveled, spread 8-10cm of coconut coir to obtain the seedbed.

[0017] Furthermore, in step (S2), the substrate formula is coconut coir: humus: perlite = 3:1:1.

[0018] Furthermore, in step (S3), the moisture content of the humus soil is 40%-50%, and it needs to be sterilized before covering with soil.

[0019] Furthermore, in step (S3), after covering with soil, cover with a shading film for 5-7 days.

[0020] Furthermore, in step (S4), after the seedbed is irrigated with root-promoting nutrient solution, it should be protected from rain for 6 hours.

[0021] Further, in step (S6), the wound healing nutrient solution is prepared as follows: 10-20g of Bletilla striata water extract, 10-20g of Phellodendron chinense bark water extract, 800-1200mg of potassium dihydrogen phosphate, and 80-120mg of indolebutyric acid are added to every 1L of water. Attached Figure Description

[0022] Figure 1 shows the appearance of a seedling obtained using the method in Example 1; Figure 2 shows the appearance of a mature seedling obtained using the seedling raising method in Comparative Example 1; Figure 3 shows the appearance of an axillary seedling separated using the method in Example 2; Figure 4 shows a comparison of the appearances of two seedlings obtained using the methods in Example 1 and Comparative Example 4 respectively; Figure 5 shows a comparison of the appearances of two seedlings obtained using the methods in Example 2 and Comparative Example 5 respectively; Figure 6 shows a comparison of the appearances of two seedlings obtained using the methods in Example 3 and Comparative Example 6 respectively; Figure 7 shows a comparison of seedlings raised using the methods in Example 1 and Comparative Example 1 and transplanted one year later; Figure 8 shows a comparison of nurseries raised using the methods in Example 1 and Comparative Example 1 respectively. Detailed Implementation

[0023] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments.

[0024] This invention discloses a root-promoting nutrient solution, formulated as follows: 150-200 mg of seaweed polysaccharide, 1.1-1.66 mg of salicylic acid, and 800-1200 mg of potassium dihydrogen phosphate are added per 1 L of water. This root-promoting nutrient solution can be applied in the seedling cultivation of Epimedium. Through a three-dimensional synergistic design of "polysaccharide water retention - salicylic acid signal regulation - phosphorus and potassium nutrient supply," this nutrient solution overcomes the limitations of traditional single-component or simply compounded nutrient solutions, shortening the rooting time of Epimedium seedlings by 30% and increasing biomass by 36.8%. The synergistic function of the three components and the innovative mechanism provide a comprehensive root cultivation system for seedling cultivation, offering an efficient, safe, and stable solution for modern agricultural precision seedling cultivation, especially suitable for large-scale production scenarios such as facility seedling cultivation and stress-based seedling cultivation.

[0025] The EC value of the root-promoting nutrient solution is 0.8-1.2 mS / cm. A root-promoting nutrient solution within this range can precisely match the physiological needs of the root system during the seedling stage, avoiding both the "damage risk" of high EC and the "nutrient deficiency" of low EC, while achieving multiple goals such as "efficient nutrient absorption," "environmental compatibility," and "stress resistance reserves." For the "seaweed polysaccharide + salicylic acid + potassium dihydrogen phosphate" compound root-promoting nutrient solution of this invention, 0.8-1.2 mS / cm... S The EC value of / cm further maximizes the synergistic effect of the three components—ensuring that the signal regulation of salicylic acid, the supply of phosphorus and potassium nutrients, and the water-retaining and bacteria-promoting effects of seaweed polysaccharides do not interfere with each other, and work together to serve the "rapid germination, robust growth, and enhanced stress resistance" of the root system, laying a key foundation for subsequent transplant survival and yield formation.

[0026] Based on the above-mentioned root-promoting nutrient solution, the present invention also provides a method for promoting root growth and seedling development of Epimedium, including the following steps: (S1) Seed pretreatment: soak Epimedium seeds in 0.05%-0.15% KNO3 solution for 20-28 hours, then soak them in 500 times carbendazim solution for 1-1.5 hours, take them out, clean them, and dry them. Stratify them in sand for 60-90 days (2-6℃), keep them moist, and germinate them at 25±1℃ until they show white sprouts.

[0027] (S2) Sowing and seedling raising: Select sparse woodland and cultivated land with relatively flat terrain, good drainage, convenient irrigation, and easy management and transportation as nursery. The nursery is built on cultivated land with a shade net with a shade degree of 60%-70%. After the nursery is leveled, make beds 1.2m wide and 0.2m high. After the bed surface is leveled, spread 8-10cm of coconut coir to obtain the seedbed.

[0028] The seeds that have sprouted and turned white in step (S1) are evenly sown on the seedbed, covered with substrate, and used for seedling cultivation; wherein, the substrate formula is coconut coir: humus fine soil: perlite = 3:1:1.

[0029] (S3) Soil induction: When the seedlings in step (S2) have unfolded 1-2 true leaves and the stem nodes have elongated to 3-5cm, completely cover the terminal bud with fine humus soil with a pH of 5.5-6.5. The soil covering thickness is 1.0-2.0cm. After covering with soil, cover with a shading film for 5-7 days.

[0030] The humus soil has a moisture content of 40%-50% and needs to be sterilized before covering. Sterilization methods include high-temperature sterilization (baking at temperatures above 100℃, etc.), chemical sterilization (using 50% carbendazim wettable powder, 70% thiophanate-methyl wettable powder, 30% hymexazol aqueous solution, etc.), and biological sterilization (composting treatment).

[0031] (S4) Rooting induction: After covering the seedbed with soil in step (S3), apply 2-4L of the above-mentioned root-promoting nutrient solution per square meter of seedbed; after applying the root-promoting nutrient solution to the seedbed, protect it from rain for 6 hours.

[0032] (S5) Separation of axillary seedlings: 30-60 days after covering the seedbed with soil in step (S4), when axillary seedlings are present, after ≥5 secondary roots have formed at the base of the axillary seedlings, transversely cut the stem node connecting the mother seedling and the new seedling at the middle, with the cut 2±0.5cm from the base of the new seedling. A sterilized blade must be used during the cutting operation, and the blade should be disinfected with 75% ethanol after every 20 seedlings are treated for subsequent processing.

[0033] (S6) Independent Culture: Spray the wound healing nutrient solution onto the incision sites of the mother seedling and new seedlings in step (S5), and culture the mother seedling and new seedlings separately until they reach maturity. The wound healing nutrient solution is prepared as follows: add 10-20g of Bletilla striata water extract, 10-20g of Phellodendron chinense bark water extract, 800-1200mg of potassium dihydrogen phosphate, and 80-120mg of indolebutyric acid per 1L of water.

[0034] The present invention has the following beneficial effects: 1. The present invention innovatively proposes to carry out precise soil covering treatment (thickness 1.0-2.0cm) during the seedling leaf unfolding stage (when 1-2 true leaves unfold), thereby activating the axillary buds of the stem nodes to develop into independent new seedlings by relieving apical dominance, promoting axillary bud development, and improving the seedling potential of a single plant.

[0035] 2. When the new seedlings have formed more than 5 secondary roots, the technique of cutting the stem node horizontally (in the middle of the stem node) can be used to make the two seedlings completely independent. Combined with the treatment of wound healing nutrient solution, double seedling cultivation can be achieved, realizing the operation of large-scale seedling cultivation in a short time.

[0036] 3. By using a newly designed root-promoting nutrient solution, the rooting time of new seedlings is shortened and the biomass is increased. Combined with precise soil covering during the leaf expansion period of Epimedium seedlings, the propagation efficiency is greatly improved compared with traditional methods. At the same time, the number of seedling roots, the number of buds and the root weight are significantly increased. Moreover, the operation is simple and the cost is low, making it suitable for large-scale industrial application and providing an innovative solution to the problem of Epimedium resource shortage.

[0037] The effectiveness of the seedling cultivation method of the present invention will be verified through the following multiple embodiments and comparative examples. Example 1

[0038] A method for promoting root growth and seedling development of Epimedium includes the following steps: (S1) Seed pretreatment: soak Epimedium seeds in 0.15% KNO3 solution for 20 hours, then soak them in 500 times carbendazim solution for 1.5 hours, take them out, clean them, and dry them. Store them in sand at a temperature of 2-6℃ and keep them moist. After stratification in sand for 80 days, germinate them at a temperature of 25±1℃ until the seeds show white sprouts.

[0039] (S2) Sowing and seedling raising: Select sparse woodland and cultivated land with relatively flat terrain, good drainage, convenient irrigation, and easy management and transportation as nursery. The nursery is built on cultivated land with a shade net with a shade degree of 60%-70%. After the nursery is leveled, make beds 1.2m wide and 0.2m high. After the bed surface is leveled, spread 8-10cm of coconut coir to obtain the seedbed.

[0040] The seeds that have sprouted and turned white in step (S1) are evenly sown on the seedbed, covered with substrate, and used for seedling cultivation; wherein, the substrate formula is coconut coir: humus fine soil: perlite = 3:1:1.

[0041] (S3) Soil induction: When the seedlings in step (S2) unfold 1-2 true leaves and the stem nodes elongate to 3-5cm, completely cover the terminal bud with fine humus soil with a pH of 5.5-6.5, with a soil covering thickness of 1.0cm, and cover with a shading film for 5 days after covering.

[0042] The humus soil has a moisture content of about 40%, and it needs to be sterilized before covering with soil. Sterilization methods include high-temperature sterilization (baking at temperatures above 50°C, etc.), chemical sterilization (using 50% carbendazim wettable powder, 70% thiophanate-methyl wettable powder, 30% hymexazol aqueous solution, etc.), and biological sterilization (composting treatment).

[0043] (S4) Rooting Induction: After covering the seedbed with soil in step (S3), irrigate it with 2L of the above-mentioned root-promoting nutrient solution per square meter of seedbed; after irrigating the seedbed with the root-promoting nutrient solution, protect it from rain for 6 hours. The preparation ratio of the root-promoting nutrient solution is as follows: add 200mg of seaweed polysaccharide, 1.2mg of salicylic acid, and 1000mg of potassium dihydrogen phosphate to every 1L of water. The EC value of the root-promoting nutrient solution is 1mS / cm.

[0044] (S5) Separation of axillary seedlings: 40 days after covering the seedbed with soil in step (S4), when axillary seedlings are present, after ≥5 secondary roots have formed at the base of the axillary seedling, transversely cut the stem node connecting the mother seedling and the axillary seedling at the middle, with the cut 2±0.5cm from the base of the new seedling. A sterilized blade must be used during the cutting operation, and the blade should be disinfected with 75% ethanol after every 20 seedlings are treated for subsequent processing.

[0045] (S6) Independent Culture: Spray the wound healing nutrient solution onto the incision sites of the mother plantlet and axillary seedlings from step (S5), and culture the mother plantlet and axillary seedlings separately until they reach maturity. The wound healing nutrient solution is prepared as follows: add 15g of Bletilla striata water extract, 15g of Phellodendron chinense bark water extract, 800mg of potassium dihydrogen phosphate, and 120mg of indolebutyric acid per 1L of water. Example 2

[0046] The difference between this embodiment and Example 1 is as follows: (S1) Seed pretreatment: Epimedium seeds were soaked in 0.05% KNO3 solution for 28 hours, then soaked in 500 times diluted carbendazim solution for 1 hour. After being taken out, cleaned, and dried, the seeds were stratified in sand at a temperature of 2-6℃ and kept moist. After stratification in sand for 60 days, the seeds were germinated at a temperature of 25±1℃ until they showed white sprouts. Example 3

[0047] The difference between this embodiment and Embodiment 1 is as follows: (S1) Seed pretreatment: Epimedium seeds were soaked in 0.1% KNO3 solution for 25 hours, and then soaked in 500 times carbendazim solution for 1 hour. After being taken out, they were cleaned and dried. The sand storage temperature was 2-6℃ and kept moist. After sand storage for 90 days, the seeds were germinated at 25±1℃ until they showed white sprouts.

[0048] (S3) Soil Covering Induction: When the seedlings from step (S2) have developed 1-2 true leaves and the stem nodes have elongated to 3-5 cm, completely cover the terminal buds with fine humus soil with a pH of 5.5-6.5, with a soil covering thickness of 1.0 cm. After covering with soil, cover with a shade film for 7 days. Example 4

[0049] The difference between this embodiment and Embodiment 1 is as follows: (S4) Rooting induction: After covering the seedbed with soil in step (S3), irrigate it with 3L of the above-mentioned root-promoting nutrient solution per square meter of seedbed; after irrigating the seedbed with the root-promoting nutrient solution, protect it from rain for 6 hours. The preparation ratio of the root-promoting nutrient solution is as follows: add 150mg of seaweed polysaccharide, 1.6mg of salicylic acid, and 1200mg of potassium dihydrogen phosphate to every 1L of water. The EC value of the root-promoting nutrient solution is 0.8mS / cm.

[0050] (S6) Independent Culture: Spray the wound healing nutrient solution onto the incision sites of the mother plantlet and axillary seedlings from step (S5), and culture the mother plantlet and axillary seedlings separately until they reach maturity. The wound healing nutrient solution is prepared as follows: add 10g of Bletilla striata water extract, 10g of Phellodendron chinense bark water extract, 1000mg of potassium dihydrogen phosphate, and 100mg of indolebutyric acid per 1L of water. Example 5

[0051] The difference between this embodiment and Embodiment 1 is as follows: (S4) Rooting induction: After covering the seedbed with soil in step (S3), irrigate it with 4L of the above-mentioned root-promoting nutrient solution per square meter of seedbed; after irrigating the seedbed with the root-promoting nutrient solution, protect it from rain for 6 hours. The preparation ratio of the root-promoting nutrient solution is as follows: add 180mg of seaweed polysaccharide, 1.1mg of salicylic acid, and 800mg of potassium dihydrogen phosphate to every 1L of water. The EC value of the root-promoting nutrient solution is 1.2mS / cm.

[0052] (S6) Independent cultivation: Spray the wound healing nutrient solution on the incisions of the mother seedling and axillary seedlings in step (S5), and cultivate the mother seedling and axillary seedlings separately until they reach maturity. The wound healing nutrient solution is prepared as follows: add 20g of Bletilla striata water extract, 20g of Phellodendron chinense bark water extract, 1200mg of potassium dihydrogen phosphate, and 80mg of indolebutyric acid to every 1L of water.

[0053] Comparative Example 1 differs from Example 1 as follows: (S3) Soil induction: When the seedlings in step (S2) have unfolded 1-2 true leaves and the stem nodes have elongated to 3-5 cm, they are covered with a shading film for 5 days. In other words, Comparative Example 1, based on Example 1, does not perform soil induction treatment on the seedlings with long true leaves.

[0054] Comparative Example 2 differs from Example 2 as follows: (S3) Soil induction: When the seedlings in step (S2) have unfolded 1-2 true leaves and the stem nodes have elongated to 3-5 cm, they are covered with a shading film for 5 days. In other words, Comparative Example 2, based on Example 2, does not perform soil induction treatment on the seedlings with long true leaves.

[0055] Comparative Example 3 differs from Example 3 as follows: (S3) Soil Covering Induction: When the seedlings in step (S2) have unfolded 1-2 true leaves and the stem nodes have elongated to 3-5 cm, they are covered with soil and then covered with a shading film for 5 days. In other words, Comparative Example 3, based on Example 3, does not perform soil covering treatment on the seedlings with long true leaves.

[0056] Comparative Example 4 differs from Example 1 as follows: (S4) Rooting Induction: After covering the seedbed with soil in step (S3), 2L of rooting nutrient solution was applied per square meter of seedbed for irrigation. The seedbed was protected from rain for 6 hours after irrigation. The rooting nutrient solution formula (1L water) consisted of: indolebutyric acid: 12mg; potassium dihydrogen phosphate: 1000mg; boric acid: 20mg.

[0057] Comparative Example 5 differs from Example 2 as follows: (S4) Rooting Induction: After covering the seedbed with soil in step (S3), 2L of rooting nutrient solution was applied per square meter of seedbed for irrigation. The seedbed was protected from rain for 6 hours after irrigation. The rooting nutrient solution formula (1L water) consisted of: indolebutyric acid: 12mg; potassium dihydrogen phosphate: 1000mg; boric acid: 20mg.

[0058] Comparative Example 6 differs from Example 3 as follows: (S4) Rooting Induction: After covering the seedbed with soil in step (S3), 2L of rooting nutrient solution was applied per square meter of seedbed for irrigation. The seedbed was protected from rain for 6 hours after irrigation. The rooting nutrient solution formula (1L water) consisted of: indolebutyric acid: 12mg; potassium dihydrogen phosphate: 1000mg; boric acid: 20mg.

[0059] The results show that the Epimedium seeds used in Examples 1, 4, 5, Comparative Examples 1 and 4 were Epimedium sagittatum seeds; the Epimedium seeds used in Examples 2, 2 and 5 were Epimedium coarse hair seeds; and the Epimedium seeds used in Examples 3, 3 and 6 were Epimedium soft hair seeds.

[0060] Data from the seedlings in Examples 1-5 and Comparative Examples 1-6 were statistically analyzed, and the summarized data are shown in Table 1: Table 1:

[0061] Note: The seedbeds in each embodiment and comparative example were approximately 0.85m long, making each seedbed about 1 square meter. The number of seedlings was recorded. Then, 10 mature seedlings were randomly picked, weighed, and the number of roots and buds was recorded. The roots were separated and weighed, and the average number of roots was calculated. Note that the number of roots mainly refers to the large root system that grows from the seed. Small branch roots on the large root system belong to the same large root system and are counted as 1. Survival rate one year after transplanting: One year after transplanting, approximately 3 square meters were randomly designated, and the number of surviving seedlings was recorded, and the survival rate was calculated. Number of buds and biomass one year after transplanting: One year after transplanting, three seedlings were randomly selected, their buds were counted, washed, dried, and weighed.

[0062] The data recorded in Table 1 show that, by comparing the data of Example 1 and Comparative Example 1, it can be seen that the soil covering operation can increase the number of seedlings by 30.8%, biomass by 11.3%, number of roots by 41.1%, number of buds by 90%, and root weight by 46.9%.

[0063] A comparison of the data from Example 2 and Comparative Example 2 shows that using soil covering can increase the number of seedlings by 16.3%, biomass by 6.1%, number of roots by 35%, number of buds by 52.4%, and root weight by 41.6%.

[0064] A comparison of the data from Example 3 and Comparative Example 3 shows that using soil covering can increase the number of seedlings by 23.8%, biomass by 6.3%, number of roots by 36.4%, number of buds by 77.8%, and root weight by 52.1%.

[0065] As can be seen from the data in Example 1 and Comparative Example 4, compared with general rooting nutrients, the root-promoting nutrient of the present invention can increase the number of seedlings by 54.5%, biomass by 12.4%, number of roots by 63.2%, bud growth by 153%, and root weight by 83.2%.

[0066] As can be seen from the data in Example 2 and Comparative Example 5, compared with general rooting nutrients, the root-promoting nutrient of the present invention can increase the number of seedlings by 67.6%, biomass by 12.3%, number of roots by 111.6%, bud growth by 166.7%, and root weight by 78.2%.

[0067] As can be seen from the data of Example 3 and Comparative Example 6, compared with general rooting nutrients, the root-promoting nutrient of the present invention can increase the number of seedlings by 44.4%, biomass by 9.6%, number of roots by 87.5%, bud growth by 146.2%, and root weight by 73.5%.

[0068] In summary, it can be seen that planting different species of Epimedium without covering them with soil has a particularly significant impact on the number of buds. This indicates that the method described in the attached diagram can significantly increase the number of buds, facilitating subsequent transplanting and enabling large-scale seedling production in a short time. Furthermore, when using general rooting nutrient solutions, the data for different Epimedium species are significantly lower than those using the root-promoting nutrient agent described in this application, especially in terms of biomass, root number, and bud count. Using the root-promoting nutrient agent described in this application has a superior effect on Epimedium cultivation, resulting in better seedling quality and increased yield.

[0069] Furthermore, through the data comparison of Example 1, Comparative Example 1, Comparative Example 4, Example 2, Comparative Example 2, Comparative Example 5, and Example 3, Comparative Example 3, Comparative Example 6, it can be seen that the operation of not covering the soil also has a slight impact compared to not using the root-promoting nutrient agent. However, the root-promoting nutrient agent has a greater impact on the seedlings. Therefore, it can also be used to illustrate the importance of the root-promoting nutrient agent of this application in the cultivation process of Epimedium.

[0070] Table 1 shows the number and biomass of buds in Example 1. Before transplanting, the number of buds increased from 3.8 to 20.68, and the biomass increased from 7.75 to 129.44 g one year after transplanting. Currently, Epimedium is used medicinally approximately two years after transplanting. Based on the growth data one year after transplanting, it can be inferred that from one year to two years after transplanting (at the time of medicinal use), the number of buds increased from 20 to 30-40. Proportionally, the biomass could increase to 194-259 g / plant (weight of stems and leaves per plant, deducting the weight of roots; stems and leaves account for approximately 60%-80% of the total biomass, and the actual medicinal weight of stems and leaves is approximately 116-207 g / plant). It can be seen that using soil covering + root-promoting nutrient solution can significantly increase the yield of Epimedium. By analogy, the planting methods of the embodiments in this application all have the characteristic of higher yield.

[0071] By comparing the data from Example 1, Comparative Example 1, and Comparative Example 4 in Table 1, it can be seen that the examples have the following advantages: 1. The yield (biomass one year after transplanting) is significantly higher. Example 1 (129.44g) > Comparative Example 1 (57.88g) and Comparative Example 4 (37.17g); Example 2 (118.49g) > Comparative Example 2 (68.47g) and Comparative Example 5 (49.43g); Example 3 (125.40g) > Comparative Example 3 (54.63g) and Comparative Example 6 (41.33g). The biomass of the examples is 1.7-3.5 times that of the corresponding examples, demonstrating a strong yield advantage.

[0072] 2. Superior seedling quality and more buds: The number of buds in Examples 1 (20.67), 2 (19.33), and 3 (22.00) is 1.8-2.1 times that of the corresponding ratio (mostly 9-12), indicating stronger reproductive potential; More stable / higher survival rate: The survival rates of Examples 1 (98.77%), 2 (100.00%), and 3 (98.77%) are generally higher than those of the corresponding ratio (mostly 96.30%-98.77%), ensuring better seedling survival.

[0073] 3. More solid foundation in the early stage: The initial biomass, root weight and other indicators at the time of planting (such as the initial biomass of 7.75g and root weight of 2.79g in Example 1) are better than the corresponding ratio, laying a better foundation for later growth.

[0074] In summary, regarding the survival rate one year after transplanting, the presence or absence of soil covering and the use of root-promoting nutrients had little impact. However, the number of buds after one year showed a significant reduction in bud count for seedlings without soil covering and without the use of root-promoting nutrients. Therefore, in terms of Epimedium yield, using a combination of soil covering and root-promoting nutrient solution can significantly increase the yield. The number of seedlings emerging per square meter in the nursery also indicates that the absence of soil covering and the lack of root-promoting nutrient solution had a substantial impact on the seed germination rate.

[0075] Note that the above growth rate is calculated as (example value - comparative value) / comparative value * 100%.

[0076] It can be seen that the core objective of this invention is to systematically improve the overall quality and robustness of Epimedium seedlings through the synergistic application of precise soil covering and induction technology and special root-promoting nutrient solution, laying a solid foundation for achieving high and stable yields.

[0077] The specific effects are reflected in the following: significantly developed root system, laying the foundation for strong seedlings: seedlings induced by this root-promoting nutrient solution have more secondary roots, grow faster, have thicker and denser root systems, and significantly increased root weight, which is 46.8% higher than the control group. The developed root structure greatly enhances the seedlings' nutrient absorption capacity and stress resistance, which is the core characteristic for forming high-quality seedlings.

[0078] Robust plants with significantly increased biomass: Compared with traditional methods, the seedlings cultivated by this method are more robust, with vigorous stem and leaf growth and a more developed root system. Compared with the control, the number of roots increased by 41.05% and the root weight increased by 46.8%, which can provide stable nutritional needs for later growth and provide a reliable guarantee for subsequent transplanting and rapid growth.

[0079] Effectively improving the double seedling rate and doubling the yield of high-quality seedlings: This method, through precise soil covering during the critical growth period of seedlings, not only optimizes seedling quality but also effectively stimulates axillary bud development, thus increasing the probability of cultivating double seedlings from a single seed. This significantly improves the production efficiency of high-quality seedlings without increasing the sowing quantity.

[0080] Plump buds and great germination potential: Seedlings cultivated by this method have plump buds and increased number. After covering with soil, the number of buds increases, showing vigorous growth potential and higher seedling value.

[0081] High uniformity and stable quality of seedlings: The seedlings cultivated by this method are uniform and of stable and controllable quality. The seedling cultivation facilities are simple, which effectively solves the pain points of traditional seedling cultivation, such as weak seedlings, uneven quality and high environmental requirements. It is very suitable for large-scale and standardized industrial applications.

[0082] In summary, this invention provides a highly efficient seedling cultivation scheme with the core objective of comprehensively improving seedling quality, while also employing effective means to increase seedling survival rate. This significantly improves propagation efficiency while ensuring seedling robustness, providing reliable technical support for solving the problem of shortage of high-quality Epimedium seedlings.

[0083] A typical case is illustrated in Figure 1, which shows a seedling (Epimedium sagittatum seedling) obtained using the method in Example 1 of this embodiment. It can be seen that the seedling has a well-developed root system and an axillary seedling structure, which has the potential for separation. Subsequent cutting and separation have been achieved. The subsequent growth of the new seedling after separation is as shown in Figure 3.

[0084] As shown in Figure 2, a seedling (Epimedium sagittatum seedling) was obtained using the seedling cultivation method in Comparative Example 1. During the planting process, the soil covering operation is not performed, which easily causes the seedling to lack the axillary seedling structure and cannot achieve subsequent cutting and separation, thus making it difficult to achieve a large number of seedling cultivation operations in a short period of time.

[0085] Figure 3 shows the appearance of axillary roots obtained using the method in Example 2. It can be seen that even axillary seedlings have enough buds and enough roots to ensure the survival rate and growth rate of the axillary seedlings after division.

[0086] As shown in Figure 4, two seedlings (Epimedium sagittatum seedlings) were obtained after being cultivated using Example 1 (right) and Comparative Example 4 (left). It can be seen that the root-promoting nutrient solution of the present invention can promote the growth of the seedling roots and produce more buds compared to general root-promoting nutrient solutions. The root-promoting nutrient solution can also promote the growth of the seedling leaves, resulting in a higher overall survival rate of the seedlings.

[0087] As shown in Figure 5, two seedlings (Epimedium coarse hair) were obtained after seedling cultivation in Example 2 (left) and Comparative Example 5 (right). It can be seen that the root-promoting nutrient solution of the present invention can promote the growth of seedling roots and produce more buds compared with general root-promoting nutrient solutions. The root-promoting nutrient solution can also promote the growth of seedling leaves, resulting in a higher overall survival rate of seedlings.

[0088] As shown in Figure 6, two seedlings (Epimedium pubescens) were obtained after seedling cultivation in Example 3 (left) and Comparative Example 6 (right). It can be seen that the root-promoting nutrient solution of the present invention can promote the growth of seedling roots and produce more buds compared with general root-promoting nutrient solutions. The root-promoting nutrient solution can also promote the growth of seedling leaves, resulting in a higher overall survival rate of seedlings.

[0089] Figure 7 shows a comparison of two seedlings (Epimedium sagittatum seedlings) obtained by raising seedlings using Example 1 (left) and Comparative Example 1 (right) one year after transplanting. It can be seen that the seedlings without soil covering have significantly inferior buds, roots, and stems after one year of transplanting compared to the seedlings transplanted with soil covering. Soil covering is beneficial for increasing the yield of Epimedium.

[0090] Figure 8 shows a comparison of nurseries cultivated using the methods of Example 1 (left) and Comparative Example 1 (right). It can be seen that the overall growth status and trend of Epimedium without soil covering are not as good as those with soil covering. The nurseries without soil covering are sparser and grow more slowly. Their overall condition is not as good as the seedlings with soil covering. This shows that soil covering is very necessary in the cultivation of Epimedium and is an effective means to increase yield.

[0091] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the invention can be implemented in other specific forms without departing from its spirit or essential characteristics. Therefore, the embodiments should be considered in all respects as exemplary and non-limiting, and the scope of the invention is defined by the appended claims rather than the foregoing description. Thus, all variations falling within the meaning and scope of equivalents of the claims are intended to be included within the present invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

Claims

1. A root-promoting nutrient solution, characterized in that: The root-promoting nutrient solution is prepared as follows: add 150-200mg of seaweed polysaccharide, 1.1-1.66mg of salicylic acid, and 800-1200mg of potassium dihydrogen phosphate to every 1L of water.

2. The root-promoting nutrient solution according to claim 1, characterized in that: The EC value of the root-promoting nutrient solution is 0.8-1.2 mS / cm.

3. A method for promoting root development and shoot growth of Epimedium, characterized in that: The process includes the following steps: (S1) Seed pretreatment: After seed priming, Epimedium seeds are stratified in sand for 60-90 days until germination occurs; (S2) Sowing and seedling raising: The germinating seeds from step (S1) are evenly sown on a seedbed, covered with substrate, and raised as seedlings; (S3) Soil covering and induction: When the seedlings from step (S2) have developed 1-2 true leaves and the stem nodes have elongated to 3-5 cm, the terminal buds are completely covered with fine humus soil with a pH of 5.5-6.5, with a soil covering thickness of 1.0-2.0 cm; (S4) Rooting induction: The seeds from step (S2) are then stratified in sand until germination occurs. (S3) After the seedbed is covered with soil, irrigate it with 2-4L of the root-promoting nutrient solution described in claim 1 or 2 per square meter of seedbed; (S5) Separation of axillary seedlings: 30-60 days after the seedbed is covered with soil in step (S4), when axillary seedlings exist, after ≥5 secondary roots are formed at the base of the axillary seedlings, cut the stem node connecting the mother seedling and the axillary seedling in the middle horizontally, with the cut 2±0.5cm away from the base of the new seedling; (S6) Independent cultivation: spray the cut of the mother seedling and the axillary seedling in step (S5) with wound healing nutrient solution, and cultivate the mother seedling and the axillary seedling separately until they become seedlings.

4. The method for promoting root development and bud growth of Epimedium seedlings according to claim 3, characterized in that: In step (S1): Seed initiation is as follows: soak Epimedium seeds in 0.05%-0.15% KNO3 solution for 20-28 hours, then soak in 500 times carbendazim solution for 1-1.5 hours, take them out, wash them clean, and then dry them.

5. The method for promoting root development and bud growth of Epimedium seedlings according to claim 3, characterized in that: In step (S2), the seedbed preparation steps are as follows: Select a relatively flat, well-drained, irrigated, and easily managed sparse woodland or cultivated land as the nursery. The nursery is built on cultivated land with a shading net that provides 60%-70% shade. After leveling the nursery, build beds that are 1.2m wide and 0.2m high. After leveling the bed surface, lay 8-10cm of coconut coir to obtain the seedbed.

6. The method for promoting root development and bud growth of Epimedium according to claim 3, characterized in that: In step (S2), the substrate formula is coconut coir: humus: perlite = 3:1:

1.

7. The method for promoting root development and bud growth of Epimedium according to claim 3, characterized in that: In step (S3), the moisture content of the humus soil is 40%-50%, and it needs to be sterilized before covering with soil.

8. The method for promoting root development and bud growth of Epimedium according to claim 3, characterized in that: In step (S3), after covering with soil, cover with a shading film for 5-7 days.

9. The method for promoting root development and bud growth of Epimedium according to claim 3, characterized in that: In step (S4), after the seedbed is irrigated with root-promoting nutrient solution, it should be kept dry for 6 hours.

10. The method for promoting root development and shoot growth of Epimedium according to any one of claims 3-9, characterized in that: In step (S6), the wound healing nutrient solution is prepared as follows: add 10-20g of Bletilla striata water extract, 10-20g of Phellodendron chinense bark water extract, 800-1200mg of potassium dihydrogen phosphate, and 80-120mg of indolebutyric acid to every 1L of water.

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