Breeding method of endangered angiosperm saruma henryi

Abstract

The invention relates to the technical field of plant breeding, and discloses a breeding method of endangered angiosperm saruma henryi, which comprises the following steps: sexual propagation treatment: treating saruma henryi seeds to obtain saruma henryi seedling groups, the treatment comprising soaking the saruma henryi seeds for 36-60 hours by using a gibberellin solution with the concentration of 150-250mg / L; single plant screening: screening excellent single plants with preset excellent characters from the saruma henryi seedling population; and cutting propagation treatment: treating the excellent single plant to realize amplification. By adopting the sexual propagation treatment step of combining stratification treatment and gibberellin solution soaking in a specific parameter range, the deep dormancy problem of the saruma henryi seeds is specifically solved, the dormancy state of the saruma henryi seeds can be effectively relieved, and therefore the high seed germination rate is obtained.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of plant breeding, in particular to a breeding method of endangered angiosperm Saruma henryi. BACKGROUND

[0002] Saruma henryi is a plant of the genus Saruma in the Aristolochiaceae family, which is a Chinese endemic genus and has only one species. This endangered angiosperm has specific scientific value in plant system evolution research, and its plant also has ornamental value and medicinal value. Due to habitat destruction and self-breeding obstacles, the number of wild populations is decreasing day by day, and effective artificial breeding is of great significance for the protection and development of germplasm resources.

[0003] In the field of plant breeding, sexual reproduction is the basis for maintaining genetic diversity of species. However, the seeds of Saruma henryi have the characteristics of deep dormancy, which leads to extremely low germination rate under natural conditions. The existing conventional seed treatment method cannot effectively break the deep dormancy, so that a sufficient number of seedlings representing the genetic diversity of the population cannot be obtained, which limits the subsequent germplasm resource screening and utilization.

[0004] The cutting technology in asexual reproduction is a common means of plant expansion, which has the advantage of stably maintaining the excellent traits of the mother plant. However, in the breeding practice of Saruma henryi, it is difficult to root by cutting. This directly affects the survival rate and later growth performance of the cutting seedlings, and limits the rapid expansion and large-scale production of excellent single plants. SUMMARY

[0005] In view of the shortcomings of the prior art, the present application provides a breeding method of endangered angiosperm Saruma henryi, which solves the problems of difficult seed germination and low rooting rate of cutting in the prior art.

[0006] To achieve the above purpose, the present application is realized by the following technical scheme: a breeding method of endangered angiosperm Saruma henryi, comprising the following steps:

[0007] Sexual reproduction treatment: treating the seeds of Saruma henryi to obtain a seedling population of Saruma henryi, wherein the treatment comprises soaking the seeds of Saruma henryi in a gibberellin solution with a concentration of 150-250 mg / L for 36-60 hours;

[0008] Single plant screening: screening excellent single plants with preset excellent traits from the seedling population of Saruma henryi;

[0009] Cutting propagation treatment: the superior single plant is treated to achieve expansion, wherein the treatment comprises soaking the cutting base of the cutting taken from the superior single plant in a GGR rooting powder solution with a concentration of 400-600 mg / L for 20-40 minutes.

[0010] According to the above technical solution: by sequentially performing the warm stratification treatment and the cold stratification treatment, the natural seasonal change condition necessary for the seed to complete after-ripening is simulated. The warm stratification treatment provides a suitable temperature and humidity environment for the embryo development and physiological after-ripening of the seed, so as to remove the physiological dormancy; the subsequent cold stratification treatment meets the low-temperature vernalization requirement necessary for the seed to break dormancy.

[0011] After the stratification treatment is completed, the seed is further treated by using a gibberellin solution with specific parameter ranges to exogenously supplement the insufficient content in the seed, so as to start the endogenous hormone necessary for germination. Gibberellin can trigger a series of physiological and biochemical reactions in the seed, and finally promote the radicle to break through the seed coat and complete the germination process.

[0012] Therefore, the technical solution of the present application does not use a single treatment method, but organically combines the physical stratification treatment and the chemical hormone treatment, and specifically and stagewise removes the double dormancy mechanism that limits the germination of the seed of Radix Curcumae, so as to obtain a high seed germination rate.

[0013] Preferably, before the sexual reproduction treatment is performed, the seed of Radix Curcumae is sequentially subjected to warm stratification treatment and cold stratification treatment.

[0014] Preferably, the warm stratification treatment is performed at 18-28℃ for 50-70 days; and the cold stratification treatment is performed at 1-8℃ for 80-100 days.

[0015] Preferably, in the cutting propagation treatment, the cutting is a semi-lignified stem segment with a length of 10-18 cm and at least one node, which is cut during the vigorous growth period of the plant.

[0016] Preferably, after the cutting propagation treatment is performed, the step of inserting the cutting into a cutting substrate for rooting management is further included.

[0017] Preferably, the step of rooting management comprises maintaining the relative humidity of the ambient air at more than 90% in the early stage after the cutting is cut to promote rooting; and gradually reducing the relative humidity of the ambient air to 70-85% after the cutting is rooted to perform hardening.

[0018] Preferably, in the single plant screening, the preset superior traits include at least one of strong plant growth vigor, strong tillering ability, or strong disease and pest resistance.

[0019] Preferably, the warm stratification treatment and the cold stratification treatment are both carried out in a stratification medium mainly composed of river sand or vermiculite.

[0020] Preferably, the cutting medium is a medium mainly composed of sand or perlite.

[0021] The present application provides a breeding method of endangered angiosperm Alstonia constricta.

[0022] 1. The present application solves the deep dormancy problem of A. constricta seeds by combining stratification treatment with gibberellin solution soaking in a specific parameter range, effectively breaking the dormancy state and obtaining a high seed germination rate.

[0023] 2. The present application effectively induces the formation of adventitious roots of cuttings by cutting semi-lignified stem segments of a specific specification at a specific period and treating them with GGR rooting powder solution in a specific parameter range, solving the technical problem of low rooting rate of conventional cuttings and obtaining a high rooting rate and strong root system.

[0024] 3. The present application adds a rooting step including high humidity promotion of rooting in the early stage and low humidity seedling raising in the later stage in the cutting propagation process, which provides a transition process for the tender root system formed after GGR treatment from formation to adaptation to the external environment, solves the technical problem of low survival rate of cuttings after rooting due to dramatic environmental changes, and thus obtains high survival rate and high health of seedlings. BRIEF DESCRIPTION OF DRAWINGS

[0025] Figure 1 The present application provides a breeding method of endangered angiosperm Alstonia constricta. DETAILED DESCRIPTION

[0026] The technical solutions of the present application will be described clearly and completely below with reference to the drawings of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0027] Please refer to the drawings of the present application Figure 1 The present application provides a breeding method of endangered angiosperm Alstonia constricta, comprising the following steps:

[0028] Sexual reproduction treatment: the seeds of Alipima sinensis are treated to obtain a seedling population of Alipima sinensis, wherein the treatment comprises soaking the seeds of Alipima sinensis in a gibberellin solution with a concentration of 150-250 mg / L for 36-60 hours; the sexual reproduction treatment further comprises sequentially performing warm stratification treatment and cold stratification treatment on the seeds of Alipima sinensis before the gibberellin solution soaking. The warm stratification treatment is performed at 18-28℃ for 50-70 days; the cold stratification treatment is performed at 1-8℃ for 80-100 days. Both the warm stratification treatment and the cold stratification treatment are performed in a stratification substrate mainly composed of river sand or vermiculite.

[0029] Single plant screening: from the seedling population of Alipima sinensis, an excellent single plant with preset excellent traits is screened; the preset excellent traits in the single plant screening include at least one of strong plant growth vigor, strong tillering ability, or strong disease and pest resistance.

[0030] Cutting propagation treatment: the excellent single plant is treated to achieve amplification, wherein the treatment comprises soaking the base of cuttings taken from the excellent single plant in a GGR rooting powder solution with a concentration of 400-600 mg / L for 20-40 minutes. The cuttings in the cutting propagation treatment are semi-lignified stem segments with a length of 10-18 cm and at least one node, which are cut during the vigorous growth period of the plant. After the GGR rooting powder solution soaking in the cutting propagation treatment, the step of inserting the cuttings into a cutting substrate for rooting management is further included. The step of rooting management comprises maintaining the relative humidity of the ambient air at more than 90% in the early stage after the cutting to promote rooting; and gradually reducing the relative humidity of the ambient air to 70-85% for seedling hardening after the cuttings root. The cutting substrate is a substrate mainly composed of sand or perlite.

[0031] Specifically, the application provides a breeding method of the endangered angiosperm Alipima sinensis, which comprises three steps of sexual reproduction treatment, single plant screening, and cutting propagation treatment.

[0032] Firstly, the sexual reproduction treatment is performed, the fruits of Alipima sinensis are collected when the fruits are mature but have not yet cracked, and the seeds are obtained and washed. After the seeds are mixed with wet river sand at a volume ratio of 1:3, the mixture is placed in a constant temperature environment at 25℃ under light-proof conditions for warm stratification treatment for 60 days to complete the physiological after-ripening. Subsequently, the mixture is transferred to an environment at 4℃ for cold stratification treatment for 90 days. After the treatment, the seeds are soaked in a gibberellin solution with a concentration of 200 mg / L for 48 hours.

[0033] The experimental results show that this step is necessary to remove the seed dormancy, in a set of comparative experiments, using an equal amount of water instead of gibberellin solution for treatment, its seed germination rate is only 12.1%; while using the gibberellin solution described in the method after treatment, the seed germination rate can reach 83.7%. Further experimental data confirm that when the concentration and soaking time of gibberellin solution vary within the range of 150-250 mg / L and 36-60 hours, for example, 150 mg / L for 36 hours or 250 mg / L for 60 hours, the seed germination rate can still reach 76.2% and 80.5% respectively. This shows that the combination of sequential warm and cold stratification treatment and gibberellin solution treatment with specific parameters can systematically solve the dual dormancy problem of physiological after-ripening and endogenous hormone deficiency of the seed of Valvata vivipara, so as to obtain a sufficient number of seedling population.

[0034] After obtaining the Valvata vivipara seedling population, single plant screening is carried out. During the growth of the seedlings, the performance of each single plant is continuously observed and recorded, and according to the indexes of plant growth rate, tillering number and whether there are disease and pest symptoms, the single plants with strong growth vigor, strong tillering ability and no disease and pests are selected as excellent single plants, and are marked for subsequent expansion.

[0035] Subsequently, the excellent single plants selected are subjected to cutting propagation treatment, and in the middle of July, semi-lignified stem segments with two complete nodes and a length of 12 cm are cut from the excellent single plants as cuttings. The base of the cutting is immersed in a GGR rooting powder solution with a concentration of 500 mg / L for 30 minutes. The GGR rooting powder solution with specific parameters in this step is the key to induce the cuttings to produce adventitious roots efficiently. Experimental data show that the rooting rate of the cuttings treated by this step reaches 92.3%, the average number of roots per single plant is 6.8, and the average longest root length is 5.4 cm; while in the comparative group using only water treatment, the rooting rate of the cuttings is only 21.6%, and the average number of roots per single plant and the average longest root length are only 1.7 and 1.3 cm respectively.

[0036] The plant growth regulators in the GGR rooting powder solution act on the cambium cells at the base of the cutting, inducing them to differentiate and form adventitious root primordia, and when the concentration and time of the GGR rooting powder solution are adjusted within the range of 400-600 mg / L and 20-40 minutes, high rooting rate and good root system development state can also be obtained.

[0037] The treated cuttings are inserted into clean perlite substrate, and the relative humidity of the environment is maintained above 90% at the initial stage of cutting, which can reduce the transpiration of the seedling leaves, maintain the water balance in the plant body, and provide the necessary conditions for root development.

[0038] After the cuttings are rooted, gradually reduce the relative humidity of the environment to 70-85% for acclimation. This process gradually adapts the root water absorption capacity and leaf water retention capacity of the seedlings to a lower humidity environment. This complete rooting management step is crucial for ensuring the final survival of the cuttings.

[0039] The experimental results confirm that the survival rate of the cuttings using this rooting management step can reach 91.4%; while in the control group lacking this specific humidity management, due to physiological water loss in the seedlings, the survival rate is only 35.7%. This cutting propagation process, through specific parameter hormone treatment and fine rooting environment management, solves the technical problems of difficult induction of adventitious roots and low survival rate after rooting, and achieves efficient expansion of excellent single plants.

[0040] Example 1:

[0041] This embodiment provides a propagation method for the endangered angiosperm Alstonia constricta.

[0042] First, perform sexual reproduction. Select healthy Alstonia constricta mother plants without pests and diseases, collect fruits when they are mature but have not yet cracked, remove impurities such as fruit skins, obtain seeds, and rinse with clean water to remove empty seeds. Mix the treated seeds with wet river sand in a volume ratio of 3:1, place them in a constant temperature of 25°C in the dark, and perform warm stratification for 60 days. After warm stratification, transfer the mixture to a 4°C environment for cold stratification for 90 days. Prepare a gibberellin solution with a concentration of 200 mg / L, immerse the stratified seeds in the solution for 48 hours. Spread the treated seeds evenly in a seedling substrate composed of grass charcoal, perlite, and vermiculite, cover with a thin layer of substrate about 0.5-1.0 cm thick, and cultivate in an environment with scattered light at a temperature of 20-25°C to obtain Alstonia constricta seedlings.

[0043] During the growth of the seedlings, continuously observe and record the growth vigor, tillering ability, and disease resistance of each individual, select individuals with fast growth, high tillering number, and no disease symptoms as excellent individuals, and mark them.

[0044] The excellent single plant is then subjected to cutting propagation treatment. In mid-July, semi-lignified stems of the current year are cut from the excellent single plant to prepare cuttings with a length of 12 cm and 2 complete nodes. A GGR rooting powder solution with a concentration of 500 mg / L is prepared, and the cuttings are immersed in the solution at a position about 3-5 cm from the base for 30 minutes. The treated cuttings are inserted into clean perlite cutting substrate. In the early stage of cutting, the relative humidity of the ambient air is maintained at more than 90% through an automatic spraying system. After the cuttings are rooted, the spraying frequency is gradually reduced, and the relative humidity of the ambient air is reduced to 70-85% for seedling hardening. After the seedling hardening is completed, the rooted and healthy seedlings are transplanted into culture soil.

[0045] Example 2

[0046] This example provides a breeding method for the endangered angiosperm Alstonia constricta. Most of the steps are the same as those in Example 1, except for some treatment parameters.

[0047] In the sexual reproduction treatment, the conditions for warm stratification treatment are 18℃ for 50 days; the conditions for cold stratification treatment are 8℃ for 80 days; and the concentration of the gibberellin solution is 150 mg / L, with a soaking time of 36 hours.

[0048] In the cutting propagation treatment, the length of the cuttings is prepared to be 10 cm; the concentration of the GGR rooting powder solution is 400 mg / L, with a soaking time of 20 minutes.

[0049] All other steps, including seed collection, substrate selection, screening criteria, rooting management, and transplantation, are the same as those described in Example 1.

[0050] Example 3

[0051] This example provides a breeding method for the endangered angiosperm Alstonia constricta. Most of the steps are the same as those in Example 1, except for some treatment parameters.

[0052] In the sexual reproduction treatment, the conditions for warm stratification treatment are 28℃ for 70 days; the conditions for cold stratification treatment are 1℃ for 100 days; and the concentration of the gibberellin solution is 250 mg / L, with a soaking time of 60 hours.

[0053] In the cutting propagation treatment, the length of the cuttings is prepared to be 18 cm; the concentration of the GGR rooting powder solution is 600 mg / L, with a soaking time of 40 minutes.

[0054] All other steps, including seed collection, substrate selection, screening criteria, rooting management, and transplantation, are the same as those described in Example 1.

[0055] Comparative Example 1

[0056] The difference compared with Example 1 is that, in the sexual reproduction treatment, instead of using gibberellin solution with a concentration of 200 mg / L to soak the stratification-treated seeds for 48 hours, an equal amount of water is used, and the remaining steps are the same.

[0057] Comparative Example 2:

[0058] The difference compared with Example 1 is that, in the cutting propagation treatment, instead of using GGR rooting powder solution with a concentration of 500 mg / L to soak the base of the cuttings for 30 minutes, an equal amount of water is used, and the remaining steps are the same.

[0059] Comparative Example 3:

[0060] The difference compared with Example 1 is that, in the cutting propagation treatment, after the cuttings are inserted into the cutting substrate, instead of performing specific high-humidity and low-humidity hardening management, they are placed in a conventional natural environment humidity for maintenance, and the remaining steps are the same.

[0061] Test Example 1: Seed Germination Rate Test

[0062] To determine the effect of the treatments described in Examples 1-3 and Comparative Example 1 on the seed germination rate of Alipelta, this test is performed.

[0063] The experimental steps are as follows:

[0064] Randomly sample from the batches of seeds completed after treatment in Example 1, Example 2, Example 3, and Comparative Example 1, respectively. Set up three replicates for each batch, and each replicate contains 200 seeds.

[0065] Uniformly spread the seeds of each replicate in the seedling trays filled with the same seedling substrate (consistent with Example 1), and cover them with 0.5-1.0 centimeters of fine substrate.

[0066] Place all the seedling trays in the same environment for cultivation, with the environmental parameters set as follows: temperature 20-25°C, air relative humidity 75-85%, and scattered light.

[0067] From the sowing day, count the number of germinated seeds in each seedling tray on the 45th day. The standard for seed germination is that the radicle breaks through the seed coat and reaches a length of 1.0 centimeter.

[0068] Calculate the seed germination rate of each replicate according to the following formula, and take the average of the three replicates as the final seed germination rate of the experimental group. Seed germination rate (%) = (number of germinated seeds / total number of sown seeds) x 100%.

[0069] The experimental results are recorded in Table 1.

[0070] Experimental group Seed germination rate (%) Example 1 83.7 Example 2 76.2 Example 3 80.5

[0071] The data in Table 1 show that the seed germination rates of Examples 1-3 were all higher than those of Comparative Example 1. Comparative Example 1, which was not treated with gibberellin solution, had a seed germination rate of only 12.1%. This indicates that although the physiological after-ripening process of *Houttuynia cordata* seeds was completed after warm-cold stratification treatment, a dormancy state caused by insufficient endogenous hormones still existed. Exogenous supplementation with a specific concentration of gibberellin solution can effectively break the dormancy state, thereby improving the seed germination rate.

[0072] Examples 1-3 involved treatment with gibberellin concentrations of 150-250 mg / L and treatment times ranging from 36-60 hours, achieving seed germination rates of 83.7%, 76.2%, and 80.5%, respectively. These data demonstrate that gibberellin treatment within this parameter range consistently yields high seed germination rates. The parameter ranges in this method provide effective technical conditions for obtaining high seed germination rates.

[0073] The sexual reproduction treatment in this technical solution systematically solves the dual dormancy problem of physiological after-ripening and insufficient endogenous hormones in *Heliotropium indicum* seeds by combining sequential warm and cold stratification treatments with subsequent gibberellin solution treatment with specific parameters. This sexual reproduction treatment step ensures that a sufficient number of genetically diverse seedlings can be obtained before the subsequent screening steps, providing a foundation for the implementation of the entire breeding method.

[0074] Test Example 2: Rooting Rate and Root Quality Test of Cuttings

[0075] This test was conducted to determine the effect of the treatments described in Examples 1-3 and Comparative Example 2 on the rooting of *Cinnamomum camphora* cuttings.

[0076] The experimental steps are as follows:

[0077] Random samples were taken from the batches of scions treated in Examples 1, 2, 3, and Comparative Example 2. Each batch was set up with three replicates, and each replicate contained 50 scions.

[0078] Each batch of cuttings was inserted into a cutting tray containing the same cutting substrate (as in Example 1) and placed in the same environment for rooting management, with environmental parameters consistent with the rooting management steps in Example 1.

[0079] Thirty days after cutting, remove each group of cuttings from the substrate and wash the base with clean water.

[0080] Count the number of cuttings that produced adventitious roots in each replicate. The standard for rooting is: at least one adventitious root with a length of not less than 1.0 cm is produced at the base of the cutting.

[0081] For rooted cuttings, count the total number of adventitious roots and measure the length of the longest adventitious root.

[0082] The rooting rate, average rooting number per plant and average longest root length of each replicate were calculated according to the following formula, and the average value of three replicates was taken as the final result of the experimental group.

[0083] Rooting rate of cuttings (%) = (number of rooted cuttings / total number of cuttings) x 100%.

[0084] Average rooting number per plant (cm) = total number of adventitious roots of all rooted cuttings in the replicate / number of rooted cuttings in the replicate.

[0085] Average longest root length (cm) = sum of the longest root length of all rooted cuttings in the replicate / number of rooted cuttings in the replicate.

[0086] The experimental results are recorded in Table 2.

[0087] Table 2: Test results of cutting rooting of each experimental group:

[0088]

[0089]

[0090] The data results of Table 2 show that the rooting rate, average rooting number per plant and average longest root length of Examples 1-3 are higher than those of Comparative Example 2. Comparative Example 2 only uses water to treat cuttings, and its rooting rate is only 21.6%, and the number of roots is small and the length is short. This indicates that for the semi-lignified stem segments of Alpinia diospyrifolia, using GGR rooting powder solution for treatment is a necessary technical condition for inducing efficient production of adventitious roots.

[0091] The plant growth regulators contained in the GGR rooting powder solution act on the cambium and parenchyma cells at the base of the cuttings, inducing cell division and differentiation, thereby forming root primordia and developing into root systems. The data of Comparative Example 2 shows that without the application of exogenous plant growth regulators, the endogenous hormone level of the cuttings is not sufficient to initiate an effective rooting process.

[0092] Examples 1-3 were treated in the range of 400-600 mg / L GGR rooting powder solution concentration and 20-40 minutes treatment time, and the rooting rate of cuttings reached more than 84%, and the number and length of roots showed good development. This indicates that the defined cutting propagation treatment parameter range in this method provides effective technical conditions for expanding the selected superior single plants, ensuring that the superior traits can be fixed and scaled up through asexual reproduction.

[0093] Test Example 3: Test of transplanting survival rate of cuttings:

[0094] To determine the effect of the rooting management methods described in Examples 1-3 and Comparative Example 3 on the transplant survival rate of the cuttings of Alpinia speciosa, this test was performed.

[0095] The experimental procedure was as follows:

[0096] From the cutting batches of Example 1, Example 2, Example 3 and Comparative Example 3, rooted seedlings of the same size were selected. Three replicates were set up for each batch, and each replicate contained 50 rooted seedlings.

[0097] All selected seedlings were transplanted into separate culture pots containing the same culture soil.

[0098] All culture pots were placed in the same greenhouse environment for maintenance, with the environmental parameters set as follows: temperature 20-25°C, air relative humidity 65-75%, and scattered light.

[0099] After 30 days of transplanting, the number of surviving plants in each replicate was counted. The standard for plant survival was that the plant did not have permanent wilting, and new leaf growth was visible at the top.

[0100] The transplant survival rate of each replicate was calculated according to the following formula, and the average of the three replicates was taken as the final transplant survival rate of the experimental group.

[0101] Transplant survival rate (%) = (number of surviving plants / total number of transplanted plants) x 100%.

[0102] The experimental results are recorded in Table 3.

[0103] Table 3: Test results of the transplant survival rate of cuttings in each experimental group:

[0104] Experimental group Transplanting survival rate (%) Example 1 91.4 Example 2 86.8 Example 3 89.1

[0105] The data results in Table 3 show that the transplant survival rates of the cuttings in Examples 1-3 are higher than that of Comparative Example 3. The cuttings in Comparative Example 3 were directly placed in the conventional natural environment humidity after rooting, and their transplant survival rate was only 35.7%. This indicates that, in the cutting propagation process, specific rooting management is a necessary technical condition for achieving high transplant survival rates.

[0106] During the initial rooting period, the newly formed root system of the cuttings has limited water absorption capacity. The rooting management steps in the examples maintain the air relative humidity above 90% at the initial stage, which reduces the transpiration of the seedling leaves and maintains the water balance in the plant body, providing time for the further development of the root system. The seedlings in Comparative Example 3 lack this high humidity environment, and their transpiration and water loss rate exceeds the water absorption rate of the root system, leading to physiological water loss and plant death.

[0107] The late-stage moisture reduction in the rooting management step allows the root system and leaf of the seedling to gradually adapt to a lower humidity environment, and completes the physiological transition from the cutting substrate to the culture soil environment. Therefore, the seedlings of Examples 1-3 treated by this step show a high survival rate after transplanting. This rooting management step solves the technical problem of low survival rate of cuttings due to environmental changes after rooting, and ensures that the rooting effect obtained by GGR solution treatment can be converted into the final survival plant.

[0108] While embodiments of the present application have been shown and described, it is to be understood that the embodiments described are merely divergences, modifications, replacements and variations of the embodiments, and the scope of the present application is defined by the appended claims and their equivalents.

Claims

1. A method for propagating the endangered angiosperm *Cinnamomum camphora*, characterized in that, Includes the following steps: Sexual reproduction treatment: The seeds of *Hylocereus undatus* were treated to obtain a population of *Hylocereus undatus* seedlings. The treatment included soaking the *Hylocereus undatus* seeds in a gibberellin solution with a concentration of 150-250 mg / L for 36-60 hours. Individual plant selection: Select superior individual plants with preset excellent traits from the above-mentioned *Houttuynia cordata* seedling population; Propagation by cuttings: The superior single plants are treated to achieve amplification, wherein the treatment includes soaking the base of the cuttings taken from the superior single plants in a GGR rooting powder solution with a concentration of 400-600 mg / L for 20-40 minutes.

2. The method for propagating the endangered angiosperm *Heliotropium indicum* according to claim 1, characterized in that: Before the sexual reproduction treatment involves soaking the seeds in gibberellin solution, the seeds are subjected to warm stratification and cold stratification treatments in sequence.

3. The method for propagating the endangered angiosperm *Heliotropium indicum* according to claim 2, characterized in that: The warm lamination treatment is performed at 18-28℃ for 50-70 days; the cold lamination treatment is performed at 1-8℃ for 80-100 days.

4. The method for propagating the endangered angiosperm *Heliotropium indicum* according to claim 1, characterized in that: The cuttings used in the propagation process are semi-lignified stem segments, 10-18 cm in length, with at least one node, taken during the vigorous growth period of the plant.

5. The method for propagating the endangered angiosperm *Heliotropium indicum* according to claim 1, characterized in that: After soaking the cuttings in GGR rooting powder solution, the propagation treatment also includes the step of inserting the cuttings into the rooting substrate for rooting management.

6. The method for propagating the endangered angiosperm *Heliotropium indicum* according to claim 5, characterized in that: The rooting management steps include maintaining the relative humidity of the ambient air above 90% in the early stage after the cuttings are inserted to promote rooting; and gradually reducing the relative humidity of the ambient air to 70-85% after the cuttings have rooted to harden off the seedlings.

7. The method for propagating the endangered angiosperm *Heliotropium indicum* according to claim 1, characterized in that: The pre-defined desirable traits in the single-plant screening include at least one of the following: strong plant growth vigor, strong tillering ability, or strong resistance to diseases and pests.

8. A method for propagating the endangered angiosperm *Heliotropium indicum* according to claim 3, characterized in that: Both the warm and cold lamination processes are carried out in a lamination matrix with river sand or vermiculite as the main components.

9. A method for propagating the endangered angiosperm *Heliotropium indicum* according to claim 6, characterized in that: The cutting substrate is a substrate with sand or perlite as the main component.

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