The invention discloses passion fruit peptide wine with high peptide content and a preparation method thereof. The passion fruit peptide wine is prepared from the following raw materials in parts by weight: 90-120 parts of fresh passion fruits, 10-20 parts of honey, 35-50 parts of brown sugar, 20-30 parts of protein powder, 0.1-0.2 part of fruit wine saccharomycetes, 0.05-0.1 part of schizosaccharomyces pombe, 0.05-0.1 part of fat yeast, 0.01-0.05 part of proteolytic enzyme and 0.05-0.1 part of flavor enzyme. The preparation method comprises the steps: cleaning and airing the fresh passion fruits, peeling, crushing to take juice, adding honey, brown sugar, fruit wine saccharomycetes, schizosaccharomyces pombe and fat yeast, fermenting, filtering to obtain filtrate and filter residues, adding protein powder decomposing enzyme and flavor enzyme into the filtrate, heating for enzymolysis, adding into the fermented filter residues, distilling to prepare wine, filtering, and aging in a jar to obtain the passion fruit peptide wine. The passion fruit peptide wine prepared by the method is excellent in flavor, contains the fragrance of passion fruits, is high in peptide content and good in nutrition, has the effects of maintaining beauty and keeping young, strengthening physique, improving immunity and the like, is suitable for people who like drinking but are afraid of drinking to injure bodies, and is relatively high in economic value.