Method for producing fuel ethanol by secondary fermentation after kudzu root steam explosion pretreatment

A fuel ethanol and pretreatment technology, applied in the direction of microorganism-based methods, biofuels, biochemical equipment and methods, etc., can solve the problems of high consumption, cooking, liquefaction, etc., and achieve the goal of reducing energy consumption, shortening the cycle, and reducing production costs Effect

Inactive Publication Date: 2010-07-14
INST OF PROCESS ENG CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The kudzu root after the optimized low-pressure steam explosion treatment can be directly fermented to produce ethanol, which solves the problem that a large amount of energy needs to be consumed for kudzu root crushing and starch cooking and liquefaction before the kudzu starch is fermented for ethanol.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: get Radix Puerariae 50g, cut into small sections, drop into steam explosion tank, process 2.5 minutes under steam pressure 0.5Mpa; In the material after processing, add glucoamylase (50U / g steam explosion dry material), at 60 After saccharification in a water bath for 30 minutes at ℃, cool to 30 ℃ and sterilize with ultraviolet light for 20 minutes. Under sterile conditions, add 0.1% (NH 4 ) 2 SO 4 , 0.1% KH 2 PO 4 and 0.10% activated yeast, and then carry out anaerobic fermentation at 30° C. for 60 h, and collect ethanol by distillation. Obtain 0.2217g ethanol / g steam explosion dry material, 4.7689g ethanol / 100g fermentation product.

Embodiment 2

[0020] Embodiment 2: Get Radix Puerariae 50g, cut into small sections, drop into steam explosion tank, process 2.5 minutes under steam pressure 0.6Mpa; In the material after processing, add glucoamylase (80U / g steam explosion dry material), at 55 After saccharification in a water bath for 60 minutes at ℃, cool to 32 ℃ and sterilize with ultraviolet light for 15 minutes. Under sterile conditions, add 0.05% (NH 4 ) 2 SO 4 , 0.1% KH 2 PO 4 And 0.25% of the activated yeast, followed by anaerobic fermentation at 32° C. for 54 hours, and ethanol was collected by distillation. Obtain 0.4057g ethanol / g steam explosion dry material, 6.6423g ethanol / 100g fermentation product.

Embodiment 3

[0021] Embodiment 3: get Radix Puerariae 50g, cut into small sections, drop into steam explosion tank, process 2.5 minutes under steam pressure 0.7Mpa; After saccharification in a water bath for 40 minutes at ℃, cool to 35 ℃ and sterilize with ultraviolet light for 18 minutes. Under sterile conditions, add 0.1% (NH 4 ) 2 SO 4 , 0.05% KH 2 PO 4 and 0.30% activated yeast, and then carry out anaerobic fermentation at 35° C. for 48 hours, and collect ethanol by distillation. Obtain 0.4205g ethanol / g steam explosion dry material, 7.6039g ethanol / 100g fermentation product.

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Abstract

The invention relates to a method for production of fuel ethanol through steam explosion pretreatment and secondary fermentation, which comprises the following steps: Kudzu roots are processed by means of steam explosion for 2 to 4 minutes under steam pressure of 0.5 to 1.0MPa; maltogenic amylase is added into the Kudzu roots after steam explosion treatment for saccharification, and (NH4)2SO4, KH2PO4 and yeasts after activation are simultaneously added into the Kudzu roots under aseptic condition for anaerobic fermentation by 48 to 60 hours; ethanol generated by fermentation is distilled and collected; steam explosion technology with short time (2 to 4 minutes) and low pressure (0.5 to 0.8) is adopted for pretreatment of the Kudzu roots, thereby the long-term cooking process (30 to 120 minutes) of starchy materials is left out and energy consumption of fermentation production of the ethanol is reduced. The Kudzu roots after steam explosion treatment are directly fermented after saccharification, and no water is needed to add otherwise, thereby the invention has the advantages of capability of improving ethanol content in beers, reduction of energy consumption of distillation, reduction of treatment of subsequent waste water, reduction of production cost, and favorability for comprehensive utilization of the Kudzu roots.

Description

technical field [0001] The process belongs to the utilization of kudzu root resources, and particularly relates to a method for producing fuel ethanol by fermentation of kudzu root after steam explosion pretreatment. Background technique [0002] Radix Pueraria (Radix Pueraria) is the underground root of Pueraria, which is a perennial leguminous vine. There are more than 30 species of Pueraria in the world, distributed in China, Japan, Southeast Asia and other countries. There are about 9 species and 2 varieties in my country, which is the distribution center of Pueraria. Pueraria resources are distributed all over the country, such as Pueraria (P.Lobata (will) Ohwi), Pueraria (P.Thomsoni Berth), Pueraria (P.edulis Pamp), Pueraria (P.Omeiensis Wang), Yunnan kudzu (P.peduncularis Crah.ex Benth), among which kudzu and kudzu are the most common and have the largest amount of resources. Pueraria is rich in starch, and the starch content of fresh pueraria reaches 18.5-27.5%, wh...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/06C10L1/02C12R1/85
CPCY02E50/17Y02E50/16Y02E50/10
Inventor 陈洪章汪卫东付小果
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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