Method for fermentative production of alcohol
A technology of alcohol and clear liquid, applied in fermentation, biofuel, etc., can solve the problems of high cost and low production efficiency, and achieve the effects of reducing the inhibition of yeast, saving costs and increasing ethanol content
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Embodiment 1
[0040] This embodiment is used to illustrate the method for producing alcohol by fermentation of the present invention.
[0041] (1) Grind 1 ton of degerminated corn with a particle size of 70% and pass it through a 20-mesh sieve, then mix it with water and 85°C, a clear liquid with a dry matter concentration of 6% by weight (weight ratio 1:0.3) to prepare a dry matter concentration of 30% The slurry (pH value is 5.2) by weight %;
[0042] (2) Add 0.07kg of Novozymes high-temperature-resistant amylase to the slurry and liquefy at a temperature of 80°C for 2 hours to obtain liquefied mash;
[0043] (3) Regulating the pH value of the liquefied mash with a concentration of 40% sulfuric acid is 4.6, and then inoculates Angelica Saccharomyces (the inoculum size is 0.1% of the volume of the liquefied mash);
[0044] (4) Add 0.12kg of acid protease (Novozymes protease), 0.002kg of yeast promoter and 1.1kg of glucoamylase to the liquefied mash after yeast inoculation and ferment for ...
Embodiment 2
[0046] This embodiment is used to illustrate the method for producing alcohol by fermentation of the present invention.
[0047] (1) Grind 1 ton of degerminated corn with a particle size of 76% and pass it through a 20-mesh sieve, then mix it with water and 85°C, a clear liquid with a dry matter concentration of 4% by weight (weight ratio 1:0.4) to prepare a dry matter concentration of 31 The slurry (pH value is 5.4) by weight %;
[0048] (2) Add 0.08kg of Novozymes high-temperature-resistant amylase to the slurry and liquefy at a temperature of 82°C for 3 hours to obtain liquefied mash;
[0049] (3) Regulate the pH value of the liquefied mash with a concentration of 40% to be 4.6, then inoculate Angelica super wine yeast (the inoculum size is 0.2% of the volume of the liquefied mash);
[0050] (4) Add 0.15kg of acid protease (Novozymes protease), 0.005kg of yeast promoter and 0.9kg of glucoamylase to the liquefied mash after inoculating yeast, and ferment for 68 hours at a t...
Embodiment 3
[0052] This embodiment is used to illustrate the method for producing alcohol by fermentation of the present invention.
[0053](1) Grind 1 ton of degerminated corn with a particle size of 85% and pass it through a 20-mesh sieve, then mix it with water and 85°C, a clear liquid with a dry matter concentration of 6% by weight (weight ratio 1:0.3) to prepare a dry matter concentration of 32 The slurry (pH value is 5.0) by weight %;
[0054] (2) Add 0.09kg of Novozymes high-temperature-resistant amylase to the slurry and liquefy at a temperature of 85°C for 3.5 hours to obtain liquefied mash;
[0055] (3) Regulating the pH value of the liquefied mash with a concentration of 40% sulfuric acid is 4.8, and then inoculates Angelica super wine yeast (the inoculum size is 0.1% of the volume of the liquefied mash);
[0056] (4) Add 0.17kg of acid protease (Novozymes protease), 0.0035kg of yeast promoter and 0.85kg of glucoamylase to the liquefied mash after inoculating the yeast, and fe...
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