A method for co-producing bioethanol and pullulan from lignocellulosic substances

The technology of lignocellulose and pullulan is applied in the field of utilizing lignocellulosic substances to jointly produce bioethanol and pullulan, and can solve the problems of ineffective utilization of xylose and high production cost of pullulan, Achieve the effect of saving resources, reducing production costs, and efficient biotransformation

Active Publication Date: 2016-08-24
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to solve the problems that xylose cannot be effectively utilized and the production cost of pullulan is too high in the current biotransformation process of cellulosic substances, and provides a method for co-producing ethanol and pullulan from lignocellulosic substances. method

Method used

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  • A method for co-producing bioethanol and pullulan from lignocellulosic substances
  • A method for co-producing bioethanol and pullulan from lignocellulosic substances
  • A method for co-producing bioethanol and pullulan from lignocellulosic substances

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preparation example Construction

[0033] The preparation method of the corn flour hydrolyzate is as follows: add 1500 g of corn flour to 4500 mL of tap water at 65-70° C., and leave it for 20 minutes to make the corn flour particles fully absorb water and swell. Add 0.9mL of liquefying enzyme (a-amylase) and liquefy at 85-90°C for 1.5h. After the liquefaction is completed, lower the temperature to 60°C, and add 3 mL of glucoamylase. Saccharification at 55-60°C for 20h. Filter the saccharification solution with a filter cloth to obtain a clear filtrate, which is the corn hydrolyzate, boil it for 10 minutes and cool it for later use.

[0034] (2) Genome rearrangement

[0035] All the cell populations of the mutant library were inoculated into YPD liquid medium, cultured to the logarithmic phase at 30° C. and 180 rpm, and the cells were collected by centrifugation. Wash the bacteria with sterile water, then put the bacteria sludge into the pre-sporulation medium (10g / L KAc, 10g / L yeast powder, 20g / L peptone), ...

Embodiment 1

[0051] Embodiment 1: the preparation of bagasse hemicellulose hydrolyzate

[0052] Remove and crush the impurities in the bagasse to 20-30 mesh, add 0.7% NaOH solution according to the solid-to-liquid ratio of 1:12 (g / mL), pretreat in the reactor at 40°C for 1.5h . At this time, the retention rate of each component of bagasse is respectively: 96.37% of cellulose, 81.02% of hemicellulose, 80.62% of lignin, and 62.93% of xylose yield. Wash the pretreated bagasse until neutral, put the raw materials into the enzymolysis buffer solution with pH 4.5-5.5 according to the solid-to-liquid ratio of 1:18 (g / mL), and add commercially available xylan at 150IU / g raw materials Enzyme, add surfactant RH (rhamnolipid) 0.005%, Tween-800.25%, PEG60000.25%, TritonX-100 (TritonX-100) 0.15%, and enzymolysis at 40 ℃ for 48h . The enzymatic hydrolysis yield of hemicellulose was 98.52%, which was 26.38% higher than that without adding surfactant. In the obtained hemicellulose hydrolyzate, the con...

Embodiment 2

[0053] Example 2: Extraction of lignin in bagasse and preparation of cellulose residue

[0054] After orthogonal optimization of the lignin extraction conditions, the optimal lignin extraction process is obtained as follows: the solid phase residue obtained in Example 1 is added to the lignin extraction mixture, the solid-liquid ratio is 1:15 (g / mL), formic acid : The ratio of ethanol is 9:1 (v / v), the temperature is 90°C, and the reaction time is 2.5h. At this time, the lignin extraction rate was 68.44%, and the lignin yield rate was 49.61%. The cellulose content in the residue after lignin extraction is 68.16%, and the others are 22.22%.

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Abstract

The invention discloses a method for combined production of bioethanol and pullulan from lignocellulose substances. The method comprises the following steps: subjecting the lignocellulose substances to alkaline-process pretreatment and xylanase enzymatic hydrolysis and then carrying out solid-liquid separation so as to respectively obtain a hemicellulose hydrolysate (xylose liquid) and a solid-phase residue; subjecting the solid-phase residue to lignin extraction so as to obtain a cellulose residue; directly applying the xylose liquid to fermentation production of pullulan (wherein yield is no less than 0.29 g of pullulan per g of xylose) and subjecting the cellulose residue to synchronous saccharification and fermentation to produce ethanol; subjecting fermentation broth containing pullulan to fermentation and distillation with fiber residue, carrying out alcohol precipitation on obtained ethanol, carrying out extraction and refining so as to obtain a pullulan product, combining ethanol-containing waste liquid produced during purifying with cellulose residue ethanol fermentation broth and carrying out distillation so as to realize recovery and reuse of ethanol. The method can realize full utilization and high-value conversion of hemicellulose and cellulose, saves resources and achieves the purposes of environmental protection and economic benefit improvement.

Description

Technical field: [0001] The invention belongs to the technical field of biotransformation of fibrous substances, and in particular relates to a method for co-producing bioethanol and pullulan by using lignocellulosic substances. Background technique: [0002] Lignocellulosic substances are composed of three parts: cellulose, hemicellulose, and lignin. It is the largest and most widely distributed renewable resource on the earth. In recent years, with the increasing depletion of mineral fuels, the utilization of lignocellulosic substances has received widespread attention. my country is a traditional agricultural country, and the content of cellulose substances is quite rich. However, for a long time, these resources have not been rationally utilized. Agricultural wastes rich in cellulose such as straw, bagasse, corncobs, and straw are usually directly incinerated or discarded. This primitive treatment method not only caused a huge waste of resources , and will bring great ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/08C12P19/14C12P19/10C12R1/645C12R1/865
Inventor 陈叶福肖冬光郭建董健李月强郭学武
Owner TIANJIN UNIV OF SCI & TECH
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