Aspergillus tubingensis L-27 strain, fermentation broth, herbicide and active component enrichment method

Abstract

The invention belongs to the technical field of herbicides, and discloses an aspergillus tubingensis L-27 strain of which a fermentation broth has herbicidal activity, the fermentation broth and an herbicide which are prepared from the strain, and an enrichment method and an identification method of herbicidal active components in the aspergillus tubingensis L-27 strain fermentation broth. By screening herbicidal activities of fermentation broths of 30 soil fungus strains, phenomena are found that inhibition rates of the aspergillus tubingensis L-27 strain fermentation broth on roots and stemsof amaranthus retroflexus, descurainia sophia and portulaca oleracea reach 100%; and regarding pharbitis purpurea, only stems grow weakly, and an inhibition rate still reaches 77.8%. A weak-base anion-exchange resin D301 is screened out to serve as an enrichment resin of the herbicidal active components, and after adsorption, growth inhibition rates of a residual liquid on roots and stems of wheat are only 1% and 7% respectively; and finally, a 100% ethanol solution of 0.1 mol / L HCL is determined to serve as a desorption agent of the active components enriched by the D301 resin. The herbicidal activity of the fermentation broth of the L-27 strain has potential development value.

Description

technical field [0001] The invention belongs to the technical field of herbicides, and in particular relates to a bacterial strain with herbicidal activity in a fermented liquid and a method for enriching the herbicidally active components in the fermented liquid. Background technique [0002] In the global pesticide market, herbicides account for about 50% of the market. It is estimated that the world herbicide market will reach 30.137 billion US dollars in 2019. However, with the progress of society and the improvement of public health awareness, the impact of extensive, long-term and large-scale use of chemical herbicides has attracted global attention. On the one hand, the application of a large number of chemical herbicides has brought environmental pollution, especially the application of long-lasting herbicides has caused residual toxicity and phytotoxicity, resulting in reduced crop production and even human and animal poisoning. At present, more than 100 chemical h...

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Problems solved by technology

However, there is no report on the herbicidal effect of Aspergillus tubingensis

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