17 results about "Amaranthus deflexus" patented technology

The invention belongs to the technical field of agricultural biology, and discloses myrothecium verrucaria with herbicidal activity and application of the myrothecium verrucaria. The strain is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC M 20251228. Experiments show that the bacterial strain has remarkable pathogenic activity on various weeds such as atrazine, ageratina adenophora, barnyard grass, amaranthus retroflexus and digitaria sanguinalis, the fatality rate of part of weeds under high spore concentration can reach 100%, and the maximum fresh weight inhibition rate can reach 87.47%. More importantly, the strain is safe to the growth of tomato crops and has no obvious adverse effect. The invention also provides application of the strain in preparation of microbial herbicides. The strain and the product thereof provide important strain resources and technical support for developing efficient, safe and environment-friendly novel biological herbicides, and are particularly suitable for green weed control in specific scenes such as tomato fields.

The application provides a pyrrolidone-containing 2-phenylpyridine derivative and a synthesis method, application and herbicide thereof, and belongs to the technical field of compound synthesis. The structural general formula of the pyrrolidone-containing 2-phenylpyridine derivative is shown in formula I or formula II. The pyrrolidone-containing 2-phenylpyridine derivative has a novel molecular skeleton, has excellent herbicidal activity on three broadleaf weeds of amaranthus retroflexus, abutilon theophrasti and medicago sativa and three gramineous weeds of euphorbia heterophylla, digitaria sanguinalis and lolium multiflorum, and has good crop selectivity on wheat, corn and rice.

The invention discloses a polyketone-like compound Japonidiene E as well as a preparation method and application thereof, and belongs to the technical field of microbial metabolites. According to the present invention, the Aspergillus japonicus TE-739D is found, the polyketone-like compound Japonidiene E is successfully separated, extracted and prepared from the metabolic fermentation product of the Aspergillus japonicus TE-739D, and through the SciFindern database retrieval and compared with the currently reported compound, the polyketone-like compound Japonidiene E is the new structure compound reported for the first time, and has the advantages of simple preparation process, low cost, low cost and the like. Functional studies find that the polyketone-like compound Japonidiene E has a strong inhibition effect on germination of amaranthus retroflexus and eleusine indica seeds, the effect of the polyketone-like compound Japonidiene E is superior to that of a positive drug glyphosate, and a technical support is provided for further development of biopesticides.

The present application relates to the technical field of soybean field amaranth resistance identification, and discloses a soybean field amaranth indoor resistance identification method.The method comprises the following steps: performing composite time sequence breaking sleep pretreatment on amaranth seeds collected from a soybean field; cultivating the pretreated seeds to the 2-4 leaf stage according to standardized parameters and screening uniform seedlings; performing gradient dose herbicide treatment on the seedlings and setting a sensitive control; calculating GR 50 values by combining images with NDVI chlorophyll spectrum, and synchronously detecting resistance related indexes; calculating corrected resistance indexes by combining correction coefficients, and outputting identification results and resistance types according to customized standards.The present application is standard in operation, lossless and efficient, can accurately determine the resistance grade and type of amaranth, and provides technical support for soybean field amaranth resistance prevention and control.

The invention provides an amaranthus retroflexus ArUGT75L6 gene, the nucleotide sequence of the amaranthus retroflexus ArUGT75L6 gene is as shown in SEQ ID NO: 1, and the coded amino acid sequence of the amaranthus retroflexus ArUGT75L6 gene is as shown in SEQ ID NO: 2. The invention also provides application of the gene. The amaranthus retroflexus ArUGT75L6 gene is used for metabolic detoxification light system II (PS) II inhibitor herbicides in plants. The relationship between the amaranthus retroflexus ArUGT75L6 gene and the photosystem II inhibitor herbicide resistance is disclosed for the first time, whether amaranthus retroflexus generates metabolic resistance to herbicides or not can be identified by detecting the expression quantity of the amaranthus retroflexus ArUGT75L6 gene, and the method has guiding significance on timely discovery of resistance, guidance of scientific pesticide application in farmland and alleviation of further spreading of pesticide resistance. The method has an important application prospect in detection and research of non-target resistance of amaranthus retroflexus to PS II inhibitor herbicides.

The invention belongs to the technical field of plant biology, and particularly relates to an amaranthus retroflexus ArCYP81Q32 gene and application thereof. The invention discovers that amaranthus retroflexus ArCYP81Q32 participates in the degradation of a metabolic detoxification protoporphyrinogen oxidase (PPO) inhibitor herbicide fluoroglycofen-ethyl in a plant body. The relationship between the amaranthus retroflexus ArCYP81Q32 gene and fluoroglycofen-ethyl resistance is disclosed for the first time, whether a plant material generates metabolic resistance to herbicides or not can be identified by detecting the expression quantity of the amaranthus retroflexus ArCYP81Q32 gene, and the method has guiding significance on timely discovery of resistance, guidance of scientific pesticide application in farmland and alleviation of further spreading of pesticide resistance. The amaranthus retroflexus ArCYP81Q32 gene detoxifies protoporphyrinogen oxidase inhibitor herbicides through metabolism, and also provides a new gene resource for developing transgenic crops resisting the herbicides.

The invention relates to a pre-emergence weeding composition and application thereof, and belongs to the technical field of pesticide compositions. The weeding composition takes pyroxasulfone and bisamide as effective components, and the mass ratio of pyroxasulfone to bisamide is (1-20): (20-1). The weeding composition can be prepared into a pesticide preparation, and the pesticide preparation comprises wettable powder, a suspending agent, a dispersible oil suspending agent, a microemulsion, an emulsion in water, granules and water dispersible granules. The pre-emergence weeding composition provided by the invention can effectively prevent and eliminate annual gramineous weeds and annual broadleaf weeds, can be safely used in wheat fields, soybean fields, peanut fields and vegetable fields, and is safe to crops and also safe to the environment. The weeding composition can be widely used for preventing and killing weeds such as lolium multiflorum, alopecurus aequalis, wild oats, bromegrass, chenopodium album, amaranthus retroflexus and shepherd's purse, and still has a relatively good prevention effect on intractable weeds.

The invention belongs to the technical field of compound synthesis, and particularly relates to a pyrazol-quinoxaline derivative and a preparation method and application thereof.The pyrazol-quinoxaline derivative is based on combination of a pyrazol structure and quinoxaline ketone, scientific and reasonable connection is carried out by screening connecting bonds, connecting sites and the like, and the pyrazol-quinoxaline derivative is obtained. The pyrazole-quinoxaline weeding drug micromolecules with relatively stable physicochemical properties and excellent druggability are prepared; the derivative especially has a remarkable post-emergence inhibition effect on barnyard grass, eleusine indica, green bristlegrass, piemarker, amaranthus retroflexus and purslane.

The invention discloses a polyketone compound japondiene F as well as a preparation method and application thereof, and belongs to the technical field of microbial metabolites. According to the present invention, the Aspergillus japonicus TE-739D is found, the polyketone compound japondiene F is successfully separated, extracted and prepared from the metabolic fermentation product of the Aspergillus japonicus TE-739D, and through the SciFindern database retrieval and compared with the currently reported compound, the polyketone compound japondiene F is the new structure compound reported for the first time, and has the following advantages that: the new structure is the new structure compound reported for the first time; functional studies find that the polyketone compound japondiene F has a strong inhibition effect on germination of amaranthus retroflexus and eleusine indica seeds, the effect of the polyketone compound japondiene F is superior to that of a positive drug glyphosate, and a technical support is provided for further development of biopesticides.

The invention discloses a polyketone compound japondiene D as well as a preparation method and application thereof, and belongs to the technical field of microbial metabolites. According to the present invention, the Aspergillus japonicus TE-739D is found, the polyketone compound japondiene D is successfully separated, extracted and prepared from the metabolic fermentation product of the Aspergillus japonicus TE-739D, and through the SciFindern database retrieval and compared with the currently reported compound, the polyketone compound japondiene D is the new structure compound reported for the first time, and has the following advantages that: the new structure is the new structure compound reported for the first time; functional studies find that the polyketone compound japondiene D has a strong inhibition effect on germination of amaranthus retroflexus seeds, the effect is better than that of a positive drug glyphosate, and technical support is provided for further development of biopesticides.

The present application relates to a kind of benzene ring alkoxy substitution sulfonylurea compound and its purposes in preparation herbicide.The compound of the present application has good prevention and removal effect on barnyard grass, elephant's ear grass, green bristlegrass, Japanese green bristlegrass, crabgrass, rape and amaranthus in 1 g / m2 dosage, soil treatment or stem-leaf treatment, and has good crop safety to wheat and rice in 2 g / m2 dosage, stem-leaf treatment or soil treatment.The compound can be used as selective herbicide in wheat field and rice field, and can also be used as herbicide in non-crop field such as forest or wasteland.The chemical structural formula of the benzene ring alkoxy substitution sulfonylurea compound is as follows:

The invention belongs to the technical field of phytochemistry, and particularly discloses a compound containing a polyhydronaphthalene skeleton and a preparation method and application thereof. The compound disclosed by the invention is prepared from a fermentation crude extract of a strain of tobacco endophytic fungus Aspergillus japonicus TE-739D, and the compound disclosed by the invention is prepared from the fermentation crude extract of the tobacco endophytic fungus Aspergillus japonicus TE-739D. Detailed structural identification is carried out on the compound through a modern spectroscopy technology, such as high-resolution mass spectrum HRESIMS and superconductive nuclear magnetic resonance spectrum NMR, and it is found that the compound contains a polyhydronaphthalene skeleton. Through SciFinderu database retrieval, it is found that the compound is a compound of a new structure, and the compound is named as japonidiene B. The compound japonidiene B has obvious herbicidal activity on amaranthus retroflexus and eleusine indica.

The invention discloses an application of a camptothecin derivative as a herbicide. The research shows that the camptothecin derivatives hydroxycamptothecin, 7-ethyl-10-hydroxycamptothecin and 9-aminocamptothecin have obvious inhibition effects on root growth and plant growth of various common typical weeds, have relatively broad-spectrum herbicidal activity, comprise dicotyledonous weeds such as amaranthus retroflexus, bidens pilosa, mikania micrantha, ageratum conyzoides, feather cockscomb and mimosa pudica, and have broad-spectrum herbicidal activity, such as amaranthus retroflexus, bidens pilosa, mikania micrantha, ageratum conyzoides, feather cockscomb and mimosa pudica. Moreover, the weeding effect is remarkable, and the weeding composition is superior to the weeding agent glyphosate which is most commonly used in agriculture; and the compound is derived from natural plants, is high in ecological safety and environment-friendly, and has very good application value and prospect in the aspect of developing herbicides. Furthermore, the camptothecin derivatives can be applied to field production through dosage form processing, and have the advantages of environmental protection and efficient utilization of resources. The application of the invention can promote diversified development of plant resources and assist sustainable agricultural development.

This invention belongs to the field of molecular biology detection technology, and provides a primer composition, gDNA, probe, and method for detecting amino acid mutation sites in ALS of Amaranthus retroflexus. This invention is the first to construct a method for detecting multiple resistance mutations at multiple sites based on the synergistic effect of multiplex PCR and PfAgo. Through multiplex PCR amplification and PfAgo specific recognition and cleavage, it achieves simultaneous detection and mutation type identification of multiple resistance target sites in the ALS gene of Amaranthus retroflexus. Based on the multiple constraint mechanism of multiplex PCR amplification, PfAgo guided sequence recognition, and fluorescent probe cleavage, it achieves stepwise identification and verification of target sites, exhibiting excellent specificity, sensitivity, and robustness, reducing the risk of false positives, ensuring accurate and reliable results, and requiring no standards for interpretation. It is suitable for rapid and simultaneous multi-site detection of resistance to ALS inhibitors in Amaranthus retroflexus in the field, is simple to operate, and has low cost, providing technical support for resistance monitoring and precision herbicide management.

The invention discloses an N-phenyl phthalimide compound containing a nicotinic acid fragment and an ester group structure as well as a preparation method and application of the N-phenyl phthalimide compound, and belongs to the field of herbicides. The technical problem that existing herbicides cannot give consideration to both herbicidal activity and use safety is solved. The structural general formula of the compound is shown as a formula (I) or a formula (II), wherein the formula (I) and the formula (II) are shown in the specification. The N-phenyl phthalimide compound containing the nicotinic acid fragment and the ester group structure provided by the invention has good inhibitory activity on PPO. The herbicidal composition has especially excellent herbicidal activity on morning glory, digitaria sanguinalis, amaranthus retroflexus, abutilon fiber and other weeds, especially has an excellent effect on dicotyledonous weeds, and the control effect is even superior to that of some commercially available commercial herbicides. The compound can be used as a PPO inhibitor. The weeding composition can also be prepared into a weeding agent for preventing and controlling crop weeds.

The present application relates to a kind of five-membered heterocyclic pyrimidine diketones compound in the preparation of herbicide purposes, especially its in the preparation of herbicide purposes for preventing and controlling dicotyledonous weed rape and amaranthus retroflexus.The compound of the present application has good prevention and control effect on dicotyledonous weed at the dosage of 100 g / acre, and the herbicidal effect on amaranthus retroflexus is 100% when stem and leaf are treated.The chemical structural formula of the five-membered heterocyclic pyrimidine diketones compound is as follows:

The application provides a 2-chloro-4-nitrophenol soluble liquid agent, which comprises the following raw materials in percentage by mass: 2-chloro-4-nitrophenol 10-20%, solvent 70-90%, emulsifier 1-15%, and cosolvent 2-10%. Based on the same inventive concept, the application further provides a preparation method of the 2-chloro-4-nitrophenol soluble liquid agent. Based on the same inventive concept, the application further provides an application of the 2-chloro-4-nitrophenol soluble liquid agent, and the weeds applied include: amaranthus retroflexus, gypsophila oldhamii, chenopodium album, erigeron bonariensis, brome, and littleseed canarygrass. The soluble liquid agent has the advantages of easy processing, easy dilution, safe and convenient use, good biological effect and the like, and the prevention effect is significantly better than that of a commercial herbicide 10% glufosinate ammonium water agent.