55 results about "Als gene" patented technology

The invention discloses a rice ALS (acetolactate synthase) mutant type protein, a mutant type gene and applications of the rice ALS mutant type protein and the mutant type gene. The amino acid sequence of the ALS mutant type protein has following mutation: the 628-poisiton amino acid corresponding to the amino acid sequence of rice ALS has the mutation. The invention further discloses a breeding method of creating herbicide-resistant rice by gene editing. The CRISPR / Cas9 gene editing technology is used for editing the ALS gene for the first time, a T-DNA-knockout new material with the stably inherited herbicide resistant characteristic can be obtained in the T2 generation through offspring screening, and basic agronomic traits of the new material have no obvious change. Compared with breeding based on chemical mutagenesis, hybrid transform breeding and the like, the orderly improved molecular breeding technology based on gene editing has the advantages of being rapid, accurate, efficient and the like, and by means of combination with gene function marked genotype selection, breeding efficiency can be greatly increased and breeding progress is substantially accelerated.

The invention discloses an ALS mutant type gene and further discloses ALS protein coded by the ALS mutant type gene and application of the ALS protein. In a rice ALS gene sequence, 75 nucleotide G changes into nucleotide C, and 339 nucleotide A changes into nucleotide C. The protein is derived from a rice mutant plant resisting an ALS inhibitor herbicide, and compared with a rice wild type ALS sequence, the protein sequence of the ALS protein mutates at the site of Gln25 or Gln113 or Ala237. If green plants express the protein sequence, the green plants can resist an acetolactate synthetase inhibitor herbicide and especially an imidazolone and sulfonylurea herbicide.

A Candida albicans bloodstream infections cause significant morbidity and mortality in hospitalized patients. Filament formation and adherence to host cells are critical virulence factors of C. albicans. Multiple filamentation regulatory pathways have been discovered, however the downstream effectors of these regulatory pathways remain unknown. The cell surface proteins in the ALS group are downstream effectors of the filamentation regulatory pathway. Particularly, Als1p mediates adherence to endothelial cells in vitro and is required for virulence. The blocking of adherence by the organism is described resulting from the use of a composition and method disclosed herein. Specifically, a pharmaceutical composition comprised of a gene, gene product, or specific antibody to the ALS gene family is administered as a vaccine to generate an immune response capable of blocking adherence of the organism.

The invention provides an escherichia coli genetically engineered bacterium for the synchronous high yield of L-tryptophan and L-valine, and application thereof. The genetically engineered bacterium is obtained through the steps that on a genome of escherichia coli, trpE (S40F) mutation is introduced while a promoter of tryptophan operon is replaced with a Ptrc promoter; an aroG (S180F) gene controlled by the Ptrc promoter is integrated to a tyrR locus; a serA (H344A, N364A) gene controlled by a Plac promoter is integrated to a yjiV pseudo gene locus; a glnA gene controlled by the Plac promoter is integrated to a ycjV pseudo gene locus; and then a bacillus subtilis alsS gene controlled by the Ptrc promoter is integrated to a yghx pseudo gene locus. Shaking flask fermentation is conducted through a strain to be able to accumulate the L-tryptophan within 22-28 h to 10-14 g / L, meanwhile the accumulation amount of valine reaches 5-7 g / L, and the total acid-producing ability is improved by50% or so compared with that of a tryptophan producing strain; and meanwhile, strain OD600 is different slightly, growth problems are avoided, but the acid-producing ability per strain is obviously improved by 120%, and effective utilization of carbon sources and cells is improved greatly.

The invention discloses a brassica napus mutator gene for resisting imidazolone weedicide and application thereof, relating to the field of plant molecule breeding. Primer pairs are utilized to carry out PCR (Polymerase Chain Reaction) proliferation by using a mutant M9 of brassica napus for resisting imidazolone weedicide as a material to obtain a mutator gene of the mutant with the sequence number of SEQ ID NO.1; and proteins are acquired by translation of the gene with an amino acid sequence of SEQ ID No.2. By applying the conventional breeding method of crossbreeding plants, backcrossing plants and the like, the gene is introduced into other napus varieties or variety series having no resistance to the imidazolone weedicide, so as to improve the durability on the imidazolone weedicide by introducing ALS gene mutation nucleotide sequence target varieties or variety series.

Transformed cells are efficiently selected using a mutant ALS gene having high specificity to PC herbicides. The transformation method comprises the steps of: transforming a host cell with a recombination vector containing a gene of interest and a gene coding for a mutant acetolactate synthase having mutation of glycine corresponds to position 95 of the amino acid sequence of a wild-type acetolactate synthase derived from rice to alanine; culturing the transformed cell obtained in the former step in the presence of a pyrimidinyl carboxy herbicide; and wherein the gene coding for the mutant acetolactate synthase is used as a selection marker.

The invention provides a specific polymerase chain reaction (PCR) primer combination for detecting an anti-amyotrophic lateral sclerosis (ALS) inhibitor herbicide beckmannia syzigachne. The combination comprises primer pairs (Seq ID No.1-4) for specific amplification of a beckmannia syzigachne ALS gene. The PCR detection method of adopting the primer combination has excellent specificity and sensitivity. The specificity test proves that the ALS gene of the beckmannia syzigachne can be amplified. The PCR method is simple and fast to operate, sampling detection can be carried out in the season on the basis of detection of nucleotide, the time from sampling to result just is 2-4 days, and rapid identification of a suspected anti-ALS inhibitor beckmannia syzigachne in a field and verification of a mutation site can be achieved by the sensitivity and the specificity, so as to guide scientific management of resistant weeds in production practice.

The invention discloses a wheat ALS mutation gene. The 331th nucleotide of an ALS gene sequence of 6BL chromosome of a wheat B genome is changed into nucleotide A from G. The invention further discloses wheat ALS mutation protein encoded by the wheat ALS mutation gene and application of the wheat ALS mutation protein. The protein is from a wheat mutant plant of an anti-ALS inhibitor herbicide. Compared with a wheat mild ALS sequence, the protein sequence mutates at a Val111 site. A green plant expresses that the mutation protein sequence can be resistant and tolerant to an acetolactate synthase inhibitor herbicide, especially an imidazolone herbicide. After 3mL of imazameth / L water (9-fold recommended concentration) is applied to a wheat seedling with one leaf and one core of the wheat ALS mutation protein, the plant can still normally grow, develop and fruit.

The invention discloses a wheat ALS mutant gene. According to the mutant gene, the 1180th nucleotide of the ALS gene sequence of the wheat B genome 6BL chromosome is changed from nucleotide G to nucleotide A. The present invention further provides a wheat ALS mutant protein encoded by the wheat ALS mutant gene and an application of the mutant protein. The mutant protein originates from wheat mutant plants capable of resisting ALS inhibition herbicide. Compared with the wheat wild type ALS order, the mutant protein order is mutated in the Ala 394 site. The expression of the protein order through a green plant can resist (endure) acetyl lactate synthase inhibitor herbicide, particularly imidazolidinone herbicide. The 1-leave-1-core wheat seedlings with the wheat ALS mutant protein can still grow, develop and bear fruits normally after an imazameth aqueous solution with a concentration of 3mL imazameth per liter of water (nine times of the recommended concentration) is applied to the seedlings.

A multiplex-polymerase chain reaction (PCR) primer is designed by a sugarcane endogenous gene ALS and an exogenous gene element (including Ubiquitin promoter, NOS promoter, NOS terminator, a Bar gene and a Bt gene). After being optimized, a multiplex-PCR reaction system is established, wherein a template which has 60% Premixtaq<TM> volume, 0.3muM primer concentration and 150ng DNA is added into the reaction system, and the annealing temperature of the reaction system is set to be 56 DEG C; a sugarcane internal standard gene (ALS gene) and five exogenous transgenic elements (including the Ubi promoter, the Bt gene, the Bar gene, the NOS promoter and the NOS terminator) can be detected at the same time by one-time PCR reaction, and the detection limit reaches 1% of the DNA content of the template. The multiplex-PCR reaction system established by the experiment not only is capable of detecting the exogenous gene composition of transgenic sugarcane, but also is capable of effectively detecting other transgenic crops.

The invention discloses a wheat ALS mutant protein. The sequences of an ALS gene of a wheat A genome 6AL chromosome, an ALS gene of a B genome 6BL chromosome and an ALS gene of a wheat D genome 6DL chromosome are mutated. The invention further discloses nucleic acid of the wheat ALS mutant protein and further discloses application of the wheat ALS mutant protein. By expressing the protein sequence, green plants can resist (tolerate) acetolactate synthetase inhibitor type herbicides, especially imidazolone type herbicides. Wheat one-leaf one-core seedlings with the wheat ALS mutant protein still can normally grow, develop and fruit after a herbicide is applied according to the concentration of 1 mL of imazameth per L of water (three times of recommended use concentration).

The invention discloses an ALS mutant type gene of japonica rice. The 536th nucleotide on an ALS gene sequence of the japonica rice is mutated into a nucleotide T from C. The invention further discloses an ALS mutant protein encoded by the ALS mutant type gene of the japonica rice and application of the ALS mutant protein. The mutant protein comes from a japonica rice mutant plant capable of resisting ALS inhibitor herbicides. Compared with a wild type ALS sequence, the protein sequence in the invention is mutated at the locus Ala179. Due to green plant expression, the protein sequence can resist (tolerate) the ALS inhibitor herbicides, particularly imidazolone herbicides.

The invention discloses an ALS mutation gene. Nucleotide, at site 75 of an ALS gene sequence of rice, of the ALS mutation gene is changed into nucleotide C from G; and / or nucleotide at side 339 is changed into nucleotide C from A; and / or nucleotide at site 710 is mutated into nucleotide T from C. The invention further discloses an ALS protein coded by the ALS mutation gene and an application thereof. The protein is from a rice mutant plant of an anti-ALS inhibitor herbicide; and compared with a rice wild type ALS sequence, the protein sequence of the ALS mutation gene generates mutation at site Gln25, Gln113 or Ala237. A green plant expresses that the protein sequence can resist acetolactic acid to synthesize the enzyme inhibitor herbicide, especially imidazolone and sulfonylurea herbicides.

The invention provides a specific polymerase chain reaction (PCR) primer combination for detecting an anti-amyotrophic lateral sclerosis (ALS) inhibitor herbicide beckmannia syzigachne. The combination comprises primer pairs (Seq ID No.1-4) for specific amplification of a beckmannia syzigachne ALS gene. The PCR detection method of adopting the primer combination has excellent specificity and sensitivity. The specificity test proves that the ALS gene of the beckmannia syzigachne can be amplified. The PCR method is simple and fast to operate, sampling detection can be carried out in the season on the basis of detection of nucleotide, the time from sampling to result just is 2-4 days, and rapid identification of a suspected anti-ALS inhibitor beckmannia syzigachne in a field and verification of a mutation site can be achieved by the sensitivity and the specificity, so as to guide scientific management of resistant weeds in production practice.

This invention relates to sunflower lines and hybrids bred to contain a highly heritable trait conferring tolerance to sulfonylurea herbicides wherein said trait is conferred by an ALS gene encoding a sulfonylurea herbicide tolerant ALS enzyme and wherein the said sulfonylurea tolerant ALS enzyme is the sulfonylurea tolerant ALS enzyme encoded within the genome of certain designated sunflower lines.