Method for endowing paddy rice with herbicide-resistant function through mutation breeding

Abstract

The invention discloses a method for endowing paddy rice with an herbicide-resistant function through mutation breeding. The method comprises the following steps: (S1) carrying out chemical mutagenesis by virtue of a chemical mutagenic agent containing 4mmol / L of sodium azide, 0.3% of nitrosomethyl urethane and 0.2% of 5-bromouracil; (S2) carrying out CO2 laser radiation induced mutation; (S3) inducing embryonic callusing; (S4) plasma radiation induced mutation: carrying out plasma radiation induced mutation on paddy rice embryonic calluses for 90s-120s, wherein the radiation power is 20W / cm<2>-30W / cm<2>, and the discharging interval is 40mm-50mm; and (S5-S7) screening M1-M3-generation ALS gene mutation type herbicide-resistant paddy rice. According to the method, by carrying out compoundinduced mutation on to-be-modified paddy rice, the mutation efficiency is improved, so that missense mutation occurs in conserved regions in ALS genomes of the to-be-modified, dislocation distributionsites are formed, an enzymatic structure and / or spatial conformation are / or changed, and then the affinity of the paddy rice to an ALS restrained herbicide is reduced.

Description

technical field [0001] The invention belongs to the technical field of mutation breeding, and in particular relates to a method for making rice have a herbicide-resistant function through mutation breeding. Background technique [0002] In recent years, various herbicides targeting acetolactate synthase (ALS) have been widely used to control weeds in rice fields due to their advantages of broad herbicidal spectrum, low toxicity, high efficiency, and strong selectivity, such as imidazole Linones, sulfonic acid ureas, triazolopyrimidines, pyrimidine thiobenzoates, sulfonamide carbonyl triazolinone herbicides, etc. [0003] ALS inhibitor herbicides form a complex by binding to the action site of acetolactate synthase in plants, blocking the substrate from entering the enzyme active site pathway, thereby inhibiting ALS activity, making branched chain amino acids such as leucine , Isoleucine, and valine synthesis are hindered, causing plant death. Although ALS inhibitor herbici...

AI Technical Summary

Problems solved by technology

Although ALS inhibitor herbicides have an efficient control effect on rice field weeds, improper application will also cause different degrees of phytotoxicity to rice, especially for sensitive rice varieties with low resistance to ALS inhibitor herbicides. Therefore, making rice resistant to ALS-inhibiting herbicides is a key factor for efficiently controlling weeds in rice fields and ensuring high and stable rice yields

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