KASP marker primers for detecting SNP mutations in rice ALS genes and application thereof

A technology of labeling primers and rice, applied in the field of molecular genetics, can solve the problems of low efficiency, long cultivation cycle, low accuracy, etc., and achieve the effect of high-throughput identification

Inactive Publication Date: 2018-02-02
HUNAN HYBRID RICE RES CENT
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, plants with herbicide resistance and their decisive substances (such as proteins, genes, etc.) rely on long-term screening, and can only be obtained with some luck. The cultivation period is long, the cost is high, the efficiency is low, and the accuracy rate is low.

Method used

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  • KASP marker primers for detecting SNP mutations in rice ALS genes and application thereof
  • KASP marker primers for detecting SNP mutations in rice ALS genes and application thereof
  • KASP marker primers for detecting SNP mutations in rice ALS genes and application thereof

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Embodiment

[0053] A kind of KASP primer of the present invention for the double SNP mutation (resulting in the change of the amino acid sequence Ser627Asn and Val643Met) in the rice ALS gene is shown in Table 1.

[0054] Table 1: KASP primers of the present invention

[0055]

[0056] In the above markers K-ALS627 and K-ALS643, PrimerX is connected to the FAM fluorescent tag sequence (the underlined part is the FAM fluorescent tag sequence), PrimerY is connected to the HEX fluorescent tag sequence (the underlined part is the HEX fluorescent tag sequence), and PrimerC is a universal primer.

[0057] The primer 1 includes the nucleotide sequence shown in SEQ ID No: 1, the primer 2 includes the nucleotide sequence shown in SEQ ID No: 2, and the primer 3 includes the nucleic acid sequence shown in SEQ ID No: 3 Nucleotide sequence; said primer 4 includes the nucleotide sequence shown in SEQ ID No: 4, said primer 5 includes the nucleotide sequence shown in SEQ ID No: 5, and said primer 6 in...

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Abstract

The invention discloses KASP marker primers for detecting simultaneous SNP mutations in Ser627Asn and Val643Met of rice ALS genes. In terms of the simultaneously generated SNP mutations in the Ser627Asn and the Val643Met of the rice ALS genes, when the phenotype of the double mutant ALS genes is labeled, the sequence of the double mutant ALS genes is a new unreported gene sequence which can primarily determine the labeled material, and then accurately determine the labeled rice material throughdouble KASP markers in the genes so as to carry out quick, highly accurate and high-throughput identification of anti-acetyl lactate synthase inhibitor herbicides of the rice. When the KASP marker primers are used for molecular marker-assisted breeding of hybrid rice varieties with herbicide resistance, the results of two pairs of KASP markers are validated mutually in order to effectively remove sterile line seedlings obtained via 'Tako' selfing by spraying herbicides.

Description

technical field [0001] The invention belongs to the technical field of molecular genetics, and in particular relates to a KASP marker primer for detecting SNP mutations in rice ALS genes and applications thereof. Background technique [0002] With the development of my country's society and economy, large rice growers generally adopt light and simple cultivation techniques, while live broadcasting technology is more popular because of saving labor and cost and increasing efficiency, and the application area is increasing year by year. Two-line hybrid rice is an original innovative technology in my country. Due to the advantages of free grouping, easy selection and strong advantages, and simplified breeding procedures, it has developed rapidly in the middle and lower reaches of the Yangtze River in my country, and its sowing area has exceeded that of "three-line" hybrid rice; but Two-line sterile lines often have problems of seed production failure or reduced seed purity cause...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 韶也赵炳然毛毕刚彭彦唐丽胡远益李曜魁张丹
Owner HUNAN HYBRID RICE RES CENT
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