SSR molecular marker A002 for identifying males and females of kiwi fruit hybrid populations
A molecular marker, kiwifruit technology, applied in the direction of DNA/RNA fragment, recombinant DNA technology, microbial determination/inspection, etc., can solve the problems of difficulty in carrying out early sex identification of kiwifruit plants, waste of breeding work, and lack of it.
Active Publication Date: 2015-01-28
WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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Problems solved by technology
Kiwifruit seedlings often flower after being planted in the ground for several years. The difference in morphological structure of kiwifruit flowers can be used to identify the sex of the plant. Therefore, a large number of fruitless male plants in the years before flowering have caused a great waste of breeding work.
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[0043] Below in conjunction with accompanying drawing and embodiment describe in detail:
[0044] Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art.
[0045] 1. Acquisition of molecular marker A002
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Abstract
The invention discloses an SSR molecular marker A002 for identifying males and females of kiwi fruit hybrid populations, relating to the technical field of molecular genetic breeding. The male plant specific fragment nucleotide sequence of the molecular marker A002 is shown in SEQ.ID No.1; the female plant specific fragment nucleotide sequence is shown in SEQ.ID No.2; and the primer sequence is shown in SEQ.ID No.3 and SEQ.ID No.4. The molecular marker A002 can be used for the early identification detection of males and females of kiwi fruits so as to avoid the great waste caused by abundant male plants in the breeding and production; by utilizing a high-density genetic map, sex related molecular markers can be rapidly explored to assist the breeding and important practical experience is provided for the development of the functional markers; and being used for marker assisted selection of males and females, the molecular marker A002 is capable of greatly improving the selection efficiency of the female plants in the breeding and shortening the breeding period, and has great application potential and relatively high economic value.
Description
technical field [0001] The invention relates to the technical field of molecular genetic breeding, in particular to an SSR molecular marker A002 used for sex identification of kiwifruit hybrid populations (A002 is named for distinguishing other SSR molecular markers, and A is the Latin name of kiwifruit Actinidia The initial letter of ), which is used for early molecular assisted selection of male and female sex traits of kiwifruit seedlings, in order to improve breeding efficiency and reduce land and labor costs. Background technique [0002] Kiwi( Actinidia chinensis Planch.) is rich in fruit vitamin C, dietary fiber and various mineral nutrients. Known as the "King of Fruits", it is a new high-grade fruit that is beneficial to health. With the rapid improvement of people's living standards, the demand for high-quality and new varieties of kiwifruit has increased significantly, and the efficiency of kiwifruit breeding needs to be improved urgently. Actinidia is a funct...
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IPC IPC(8): C12N15/11C12Q1/68
Inventor 黄宏文张琼钟彩虹龚俊杰刘春燕
Owner WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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