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Tissue-culturing quick propagation method of wild rhizoma panacis japonici

A technology of tissue culture and bamboo ginseng, which is applied in the field of plant tissue culture, can solve the problems of slow propagation speed of bamboo ginseng seeds, achieve the effects of saving rare and endangered plants, short production cycle, and overcoming generational variation

Inactive Publication Date: 2006-12-27
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

And bamboo ginseng seeds reproduce slowly

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: the tissue culture rapid propagation method of this wild bamboo ginseng, concrete steps are as follows:

[0031] 1) Selection and sterilization of explants: take the stems of wild bamboo ginseng, and use them as explants for tissue culture after surface sterilization;

[0032] 2) Callus induction culture: under sterile conditions, inoculate the cut stems (1cm long) on ​​the induced callus medium of MS+2,4-D 1.0mg / L+sucrose 30g / L, and form by induction embryogenic callus;

[0033] 3) Callus proliferation culture: embryogenic callus is cultured on the proliferation medium of 1 / 2MS+2,4-D 1.0mg / L+KT 0.2mg / L+sucrose 20g / L to form embryogenic callus ;

[0034] 4) Embryoid body differentiation culture: embryogenic callus was cultured on the differentiation medium of 1 / 2MS+2,4-D 1.0mg / L+KT 1.0mg / L+sucrose 20g / L, and the embryogenic callus Surface differentiation of embryoid bodies;

[0035] 5) Embryoid body proliferation culture: the embryoid body with embryog...

Embodiment 2

[0040] Embodiment 2: the tissue culture rapid propagation method of this wild bamboo ginseng, concrete steps are as follows:

[0041] 1) Selection and sterilization of explants: take the leaves of wild bamboo ginseng, and use them as explants for tissue culture after surface sterilization;

[0042] 2) Callus induction and proliferation culture: under sterile conditions, inoculate leaf slices (1cm×1cm) in MS+2,4-D 1.0mg / L+KT 0.1mg / L+sucrose 30g / L to induce callus On tissue culture medium, embryogenic callus is formed by induction;

[0043] 3) Callus proliferation culture: embryogenic callus is cultured on the proliferation medium of 1 / 2MS+2,4-D 1.0mg / L+KT 1.0mg / L+sucrose 20g / L to form embryogenic callus ;

[0044] 4) Embryoid body differentiation culture: embryogenic callus was cultured on the differentiation medium of 1 / 2MS+2,4-D 0.2mg / L+KT 0.2mg / L+sucrose 20g / L, and the embryogenic callus Surface differentiation of embryoid bodies;

[0045] 5) Embryoid body proliferation ...

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Abstract

The invention relates to a method for tissue culture and rapid reproduction of wild P.japonicus, comprising (1)selection and sterilization of explants: selecting stems and leaves of wild P.japonicus, surfacely sterilizing same, as explants for tissue culture; (2)callus culture; (3)callus proliferation to form embryogenic callus; (4)somatic embryos differentiation: somatic embryos differentiate from the surface of embryogenic callus; (5)somatic embryo proliferation to differentiate somatic embryos further; (6)plants regeneration culture to form full plants with roots, stems and leaves; (7)strong seedlings culture; (8)plants transplantation. The invention is provided with high frequency of regeneration plants, fast speed of proloferation, and no shortcoming of easy variation of offspring reproduced by normal seeds.

Description

technical field [0001] The invention relates to plant tissue culture technology, and mainly relates to a tissue culture rapid propagation method of wild bamboo ginseng. Background technique [0002] Bamboo ginseng (Panax japonicus C.A.Mey.) is a plant of the genus Panax japonicus of Araliaceae, also known as bamboo ginseng, northern notoginseng, and white notoginseng. It is a species recorded in the Pharmacopoeia of the People's Republic of China (2000 edition). It is used as medicine with rhizomes and fleshy roots. It is warm in nature, sweet in taste and slightly bitter. It has the effects of nourishing and strengthening, dissipating blood stasis and relieving pain, stopping bleeding and eliminating phlegm. The rhizome is used for promoting blood circulation and relieving pain, and its medicinal effect is better than that of Panax notoginseng; the fleshy root is nourishing and strong, and can replace ginseng. It is often used to treat weakness after illness, tuberculosis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N5/04
Inventor 徐刚汪一婷牟豪杰吕永平陈剑平计东风洪林
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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