Tissue culture method of Hosta plantiginea Ascherson
A technique of tissue culture and hosta, applied in the field of plant tissue culture, can solve the problems of unreported white flower hosta tissue culture technology, and achieve the effects of reducing the probability of variation, simple operation, and high reproduction coefficient
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Embodiment 1
[0019] 1. In the spring, cut out the young shoots with a length of about 1 cm that were newly grown in that year, rinse them with washing powder water for 5 minutes, and then rinse them with running water for 30 minutes, then treat them with 70% alcohol for 10 seconds on the ultra-clean workbench, and then rinse them with 0.1% liters of alcohol. Soak in mercury and shake for 6 minutes, wash with sterile water 6 times, blot dry with filter paper, insert induction medium: MS+BA 0.2mg / L +NAA 0.01mg / L , additional agar powder 0.6%, 3% sucrose. The culture temperature is 20°C, the light intensity is 2000 Lux, and the light is 14 hours a day. After 35 days, a sterile vaccine was basically formed. The pollution rate is 28%, and the seedling rate is 100%.
[0020] 2. Peel off the outer expanded leaves of the test-tube plantlets and inoculate them in MS+BA 2mg / L +NAA 0.1mg / L New axillary buds germinated from bare leaf scars in about 15 days. After 35 days, when most of the axilla...
Embodiment 2
[0023] Repeat the process of Example 1, and add hormones BA0.5mg / L and NAA0.05mg / L in the induction medium. The seedling rate is 100%.
[0024] Proliferation medium is MS+BA 3mg / L +NAA 0.3mg / L , bud differentiation number 3.67.
Embodiment 3
[0026] The process of Example 1 was repeated, and the culture temperature was 23° C. during the day and 20° C. at night. Adding hormones BA0.8mg / L and NAA0.05mg / L in the induction medium, the seedling rate was 100%.
[0027] Proliferation medium is MS+BA 3mg / L +NAA 0.5mg / L , can be transferred once in 30 days, and the number of bud differentiation is 3.58.
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