Tissue culture method of Hosta plantiginea Ascherson

A technique of tissue culture and hosta, applied in the field of plant tissue culture, can solve the problems of unreported white flower hosta tissue culture technology, and achieve the effects of reducing the probability of variation, simple operation, and high reproduction coefficient

Inactive Publication Date: 2007-05-23
沈阳市农业科学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are only a small amount of reports on the germplasm resources of the genus Hosta and garden applications, tissue culture of ornamental hosta species in various domestic literatures, but there is no report on the tissue culture technology of the white flower hosta

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1. In the spring, cut out the young shoots with a length of about 1 cm that were newly grown in that year, rinse them with washing powder water for 5 minutes, and then rinse them with running water for 30 minutes, then treat them with 70% alcohol for 10 seconds on the ultra-clean workbench, and then rinse them with 0.1% liters of alcohol. Soak in mercury and shake for 6 minutes, wash with sterile water 6 times, blot dry with filter paper, insert induction medium: MS+BA 0.2mg / L +NAA 0.01mg / L , additional agar powder 0.6%, 3% sucrose. The culture temperature is 20°C, the light intensity is 2000 Lux, and the light is 14 hours a day. After 35 days, a sterile vaccine was basically formed. The pollution rate is 28%, and the seedling rate is 100%.

[0020] 2. Peel off the outer expanded leaves of the test-tube plantlets and inoculate them in MS+BA 2mg / L +NAA 0.1mg / L New axillary buds germinated from bare leaf scars in about 15 days. After 35 days, when most of the axilla...

Embodiment 2

[0023] Repeat the process of Example 1, and add hormones BA0.5mg / L and NAA0.05mg / L in the induction medium. The seedling rate is 100%.

[0024] Proliferation medium is MS+BA 3mg / L +NAA 0.3mg / L , bud differentiation number 3.67.

Embodiment 3

[0026] The process of Example 1 was repeated, and the culture temperature was 23° C. during the day and 20° C. at night. Adding hormones BA0.8mg / L and NAA0.05mg / L in the induction medium, the seedling rate was 100%.

[0027] Proliferation medium is MS+BA 3mg / L +NAA 0.5mg / L , can be transferred once in 30 days, and the number of bud differentiation is 3.58.

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PUM

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Abstract

The invention relates to a method for cultivating jade needle organism, wherein it comprises that: using fresh bud, via initial cultivation to form germ-free seed, without cure organism to induce the bud to increment, to root and cultivate. The invention has simple process and high yield.

Description

technical field [0001] The invention relates to a hosta flower tissue culture method, belonging to the technical field of plant tissue culture. Background technique [0002] Hosta is native to the East and is widely used around the world as a garden ornamental plant and medicinal plant. Hosta plantiginea (Hosta plantiginea Ascherson), also known as Yuchun stick, Baihexian, and Baicalyx, is the only type of hosta plants with white flowers and a strong aroma. The whole plant is slightly poisonous, and the flowers have the effect of clearing away heat and nourishing the lungs. But white hosta is propagated by ramets in nature, and the growth and reproduction speed is very slow, which restricts its wide application. If it can be propagated by tissue culture, it is expected to produce a large amount of white hosta test-tube seedlings with the same character in a short period of time. [0003] At present, there are more systematic and in-depth researches on the classification, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N5/04
Inventor 赵江雷潘菊张同臣卢文经马文宏张健朴凯伟甄广田张曼丽
Owner 沈阳市农业科学院
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