44results about How to "Reduce the chance of mutation" patented technology

The invention relates to a high-yield planting method of salix integra. The method comprises the steps of (1) treating cutting planting and seedling soil; (2) selecting cutting branches; (3) treating the cutting branches; (4) cutting; (5) managing after cutting; (6) transplanting; (7) daily managing. With the adoption of the method, the survive rate of the treated salix integra is high; diseases and insect pests are reduced; a large amount of seedlings per unit area can be cultured; the land can be saved; the economic benefit is high.

The invention relates to a high-survival-rate azalea seedling cultivating method. The method comprises the step one of cuttage planting soil treatment, the step two of cuttage branch pretreatment, the step three of cuttage branch treatment, the step four of cuttage and the step five of management after cuttage. Before planting, liquid traditional Chinese medicine is used for treatment, the disease resistance ability of azalea seedlings is improved, and branches can be promoted for germinating and surviving; as the length of the cuttage branches is reduced, the number of bacteria on the seed branches is reduced, the mutant probability of the seedlings is reduced, the strain genuineness of azalea can be kept, the applicability of the azalea is enhanced, and the survival rate of the seedlings is increased.

The invention discloses a method for producing avilamycin by fermenting. The method includes steps of preparing streptomycesolivaceoviridis with inclined surface with spores, connecting spores on the inclined surface to a seed bottle to culture to obtain seed solution, preserving the seed solution in hydraulic hydrogen; inoculating the frozen seed solution to a seed culture medium to culture to obtain the seed solution; inoculating the seed solution to a fermenting culture medium to be subjected to fermenting culture by the inoculation quantity of 5wt%; and further treating the fermented liquid obtained after fermentation to obtain crystals of avilamycin. The method is easy to carry out, completed seed making process is omitted, degeneration is reduced, contamination risk is lowered, inoculation quantity is easy to control, repeatability is good, fermenting results are good, and production cost is low. Further, the fermenting effect is higher than that of the prior art, and the outputof avilamycin can be over 6500 microgram/microliter.

The invention discloses a method for prolonging the subculturing interval time of tissue culture seedlings of apple. A culture medium comprises the following components: 0.5-0.75 mg/L of MS+BA, 0.05 mg/L of NAA, 40.0 g/L of white granulated sugar and 6.0 g/L of agar; the packing quantity is 40-50% more than that in conventional breeding, and a bottleneck is sealed by using a plastic sealing film; tissue culture seedlings of apple are transferred to the culture medium and slowly grow about 2 weeks under the culture conditions that the temperature is 22-28 DEG C, the illumination intensity is 2000 lx, and the illumination time is 10-14 h/d, and then the tissue culture seedlings of apple are placed in a chemical agent storage box with a temperature of 2-8 DEG C so that the growth is slowed down; and when the culture medium is approximately dried, the tissue culture seedlings of apple are taken out of the refrigerated container to grow normally for 1-2 weeks or are directly transferred to a new culture medium. In such a way, the subculturing interval time of tissue culture seedlings of apple can be successfully prolonged from 2-3 months to 15-18 months, the survival rate of stem tips is more than 90%, the workload of subinoculation is greatly reduced, and the mutation probability is also reduced. The technique is applied to the germplasm preservation of plantlets of apples and other parts of orchardfruits and flowers, is simple, convenient, saving, and high in efficiency, and provides a new way for the preservation of germplasm resources.

The invention relates to a culture and cryopreservation method of amniotic mesenchymal stem cells. The culture method comprises amniotic tissue separation, amniotic mesenchymal stem cell separation, P0 generation amniotic mesenchymal stem cell culture and amplification culture. According to the culture method of the amniotic mesenchymal stem cells, at a separating stage of the amniotic mesenchymalstem cells, a special mixed enzyme digestive juice system (the final concentrations of components in the digestive juice are as follows: 1.5-2U/mL of neutral protease, 0.5mg/mL of deoxyribonuclease Iand 1mg/mL of collagenase IV) is adopted for digestion, so that the amniotic mesenchymal stem cells can be more effectively separated from amniotic tissues, and then the yield of P0 generation cellsis obviously improved. Compared with conventional two-step digestion, the culture method provided by the invention has the advantages that a one-step digestion method is adopted, operation is more convenient, and the cultured amniotic mesenchymal stem cells have good activity, high yield, high purity and good repeatability.

The invention discloses a preservation method of soybean virus. The preservation method provided by the invention comprises the following steps: a virus preserving fluid is adopted for preserving soybean virus; the virus preserving fluid comprises a polymer in the formula (I) shown in the specification and ascorbic acid; and n represents a natural number ranging from 20 to 70. Experiments prove that the soybean virus preserved by using the preservation method provided by the invention can completely maintain activities within three years under an extra low temperature of -80 DEG C, and can be taken as a drug source for being used directly at any time without need of excitation, the efficiency for identifying the soybean virus is improved greatly, unnecessary subcultures during the multiplication process of the mosaic virus are reduced, mutual cross infection of virus seeds can be avoided, the probability of virus variation is lowered, and the genetic stability of viruses can be kept. Therefore, the preservation method provided by the invention has a significant application value.

The invention discloses a method for preserving and taking potato germplasm resources. The method comprises the following steps that S1, single sections of potato tissue culture seedlings are shearedand cut on a culture medium in a culture bottle, and a bottle opening is sealed by using a sealing film; S2, subculture is performed on the potato tissue culture seedlings treated in step S1 at the temperature of 20-22 DEG C, the illumination intensity of 3,200-3,500 lx and the illumination time of 15-18 hours per day; after two months, the illumination intensity is reduced to 2,300-2,500 lx, andculture is continued; after two months, the illumination intensity is increased to 2,700-3,000 lx; and after two months, microtuber is naturally fruited, the illumination intensity is reduced to 0, and continuous culturing is carried out until the microtuber passes away dormancy and starts to germinate; and S3, the microtuber starting to germinate obtained in step S2 is inoculated to a culture medium in a culture bottle, a bottle opening is sealed by using a sealing film, and a new round of germplasm resource preservation is carried out according to the operation in step S2 when the microtubergrows into a new test-tube plantlet. The method for preserving and taking potato germplasm resources is simple, convenient, easy to operate, low in cost and good in effect.

The invention relates to a mixed protease system used for gene cloning and assembling, a kit containing the mixed protease system and a use method of the kit. By utilizing the mixed protease system, specificity recombinant reaction of two or more DNA can be completed at the high temperature of 40-70 DEG C. The invention also relates to the kit containing the mixed protease system. Compared with enzyme or enzyme system with the same function, the mixed protease system keeps high activity at the high temperature of 40-70 DEG C, then the specificity of recombinant reaction is improved, generation of meaningless mutation is effectively avoided, high fidelity cloning and assembling of two or more DNA can be realized, the stability is good, the reaction precision is high, and operation is simple, convenient and efficient.

The invention belongs to the technical field of medicines, and particularly relates to application of glutamine in preparation of a drug for inhibiting escherichia coli from generating gene mutation.According to the application, the glutamine and ampicillin are combined for use, and after multiple passages, the escherichia coli can be inhibited from generating drug-resistant gene mutation, so that mutation probability of drug-resistant genes is remarkably reduced, the increase times of bacteria on the lowest inhibitory concentration of the ampicillin after passage is reduced, and the survivalrate is reduced. Animal experiments also prove that passage bacteria added with the glutamine and the ampicillin are easier to remove by the body, so that the effect of slowing down the drug-resistant gene mutation of the escherichia coli on the ampicillin is achieved.