Method of prolonging preservation time of grape tissue culture seedling with mineral oil coverage

Abstract

The invention discloses a method of prolonging the preservation time of a grape tissue culture seedling with mineral oil coverage. The method includes the steps of removing leaves from tender shoots of the grape tissue culture seedling, cutting the grape tissue culture seedling into single-bud stem segments in about 1.5 cm, planting the single-bud stem segments into a subculture culture medium, covering sterilized mineral oil on the culture medium with the thickness being about 5-6 cm, sealing the culture medium with a sealing film, and culturing the single-bud stem segments under common culture conditions (temperature: 25 + / - 3 DEG C, illumination intensity: about 2000 lx, and illumination time: 10-14 h / d). By means of the method, the preservation time of the grape tissue culture seedling can be prolonged from 4 months to longer than 15 months, thereby greatly reducing subculture planting work load and reducing mutation rate. After the mineral oil is removed, the growth of the preserved tissue culture seedlings can be recovered without washing the tissue culture seedling. The method is used for germplasm preservation of the grape tissue culture seedling, is wide in available varieties, is long in preservation time, is low in cost since the mineral oil can be reused, is simple in operation, allows growth to be recovered easily and is an excellent method of in-vitro preservation of grape germplasm.

Description

Technical field [0001] The invention relates to a method for in vitro preservation of grape germplasm resources by using tissue culture technology. Background technique [0002] Due to population growth, industrial pollution, environmental degradation and other reasons, some rare species are facing extinction or endangered, coupled with artificial selection and the promotion of improved varieties, planting varieties become simplistic, many valuable and precious germplasm resources are lost, and plant germplasm resources have been preserved. Become a topic of global concern. [0003] In vitro preservation of germplasm resources is the preservation of plant explants in tissue culture under a sterile environment. It has a high reproduction coefficient and can reproduce the preservation materials in large quantities when needed; it can overcome the shortcomings of the field preservation method and does not occupy land. Elimination of field management; protection from natural disasters...

AI Technical Summary

Problems solved by technology

The tissue culture seedlings of woody plants such as apples are placed in a refrigerator, and the effect of delaying growth after lowering the cultivation temperature is very good, but the cold resistance of grapes is poor, and most varieties cannot adapt to the conditions of refrigerators below 10°C for a long time

Adding growth retardants such as PP333 to the medium or adding mannitol to increase the osmotic pressure of the medium can effectively inhibit the growth of grape tissue culture seedlings. Only about 3 months of storage time can be extended

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